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The map label for this gene is lexA

Identifier: 52785786

GI number: 52785786

Start: 1976777

End: 1977400

Strand: Reverse

Name: lexA

Synonym: BLi02032

Alternate gene names: 52785786

Gene position: 1977400-1976777 (Counterclockwise)

Preceding gene: 52785793

Following gene: 52785769

Centisome position: 46.83

GC content: 50.16

Gene sequence:

>624_bases
ATGACGAAGCTATCAAAAAGACAACTTGATATTTTGCGTTTCATTAAAGAAGAAGTGAAGCGCAAAGGCTATCCGCCTTC
CGTCAGGGAGATCGGAGAAGCGGTCGGCCTGGCGTCAAGTTCAACGGTACACGGCCACTTGGCAAGGCTTGAAACGAAGG
GATTGATCAGAAGGGATCCGACGAAGCCGAGAGCGATCGAAATTCTCGATACAGAGGAGGAAATTCATATTCCGAAAAAC
CAGGTTGTCAATGTCCCGGTCATCGGAAAGGTGACCGCCGGTACACCGATTACCGCAGTCGAAAACATCGAGGAATACTT
TCCGCTTCCCGAACGCCTGGCGCCGCCGGATGAGCACGTCTTCATGCTGGAGATCATGGGAGAAAGCATGATCGATGCCG
GAATTCTTGATCAAGATTATGTGATTGTCAAGCAGCAGAACACCGCAAACAACGGGGATATCGTCGTTGCGATGACCGAA
GACGACGAAGCGACTGTTAAGCGGTTTTTCAAGGAGGAAACCCATATCCGCCTTCAGCCGGAAAACCCGACGATGGAGCC
GATCATCCTTCAGAATGTGACGATTCTCGGAAAAGTCATCGGCGTTTTTCGCACTGTTCATTAA

Upstream 100 bases:

>100_bases
AACAAATGTTTCTGTCAATCGTTTTTTTCGAACCTATGTTTGTACTCAGAGGAAAAACATGCTATAATCTAGCTAAAATC
GACGTTTTGAGGTGCAAAAA

Downstream 100 bases:

>100_bases
AACACGGACTCATCAGCAAAACCGCCGGTCGGCGGTTTTTTTGCACGAAAAAACAGGCTCACATCCATTCGGCAATGAAG
GATGCGAACCTGAAACAGAG

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MTKLSKRQLDILRFIKEEVKRKGYPPSVREIGEAVGLASSSTVHGHLARLETKGLIRRDPTKPRAIEILDTEEEIHIPKN
QVVNVPVIGKVTAGTPITAVENIEEYFPLPERLAPPDEHVFMLEIMGESMIDAGILDQDYVIVKQQNTANNGDIVVAMTE
DDEATVKRFFKEETHIRLQPENPTMEPIILQNVTILGKVIGVFRTVH

Sequences:

>Translated_207_residues
MTKLSKRQLDILRFIKEEVKRKGYPPSVREIGEAVGLASSSTVHGHLARLETKGLIRRDPTKPRAIEILDTEEEIHIPKN
QVVNVPVIGKVTAGTPITAVENIEEYFPLPERLAPPDEHVFMLEIMGESMIDAGILDQDYVIVKQQNTANNGDIVVAMTE
DDEATVKRFFKEETHIRLQPENPTMEPIILQNVTILGKVIGVFRTVH
>Mature_206_residues
TKLSKRQLDILRFIKEEVKRKGYPPSVREIGEAVGLASSSTVHGHLARLETKGLIRRDPTKPRAIEILDTEEEIHIPKNQ
VVNVPVIGKVTAGTPITAVENIEEYFPLPERLAPPDEHVFMLEIMGESMIDAGILDQDYVIVKQQNTANNGDIVVAMTED
DEATVKRFFKEETHIRLQPENPTMEPIILQNVTILGKVIGVFRTVH

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=209, Percent_Identity=34.4497607655502, Blast_Score=117, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_BACLD (Q65J42)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079198.1
- RefSeq:   YP_091615.1
- ProteinModelPortal:   Q65J42
- STRING:   Q65J42
- MEROPS:   S24.001
- EnsemblBacteria:   EBBACT00000057792
- EnsemblBacteria:   EBBACT00000058650
- GeneID:   3031281
- GeneID:   3100705
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi02032
- KEGG:   bli:BL00127
- NMPDR:   fig|279010.5.peg.2730
- eggNOG:   COG1974
- GeneTree:   EBGT00050000002279
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- ProtClustDB:   PRK00215
- BioCyc:   BLIC279010-1:BLI02032-MONOMER
- BioCyc:   BLIC279010:BL00127-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 23264; Mature: 23133

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: ACT_SITE 129-129 ACT_SITE 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLSKRQLDILRFIKEEVKRKGYPPSVREIGEAVGLASSSTVHGHLARLETKGLIRRDP
CCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
TKPRAIEILDTEEEIHIPKNQVVNVPVIGKVTAGTPITAVENIEEYFPLPERLAPPDEHV
CCCCEEEEEECCCCEECCCCCEEECCEEEEECCCCCCHHHHHHHHHCCCHHHCCCCCCCE
FMLEIMGESMIDAGILDQDYVIVKQQNTANNGDIVVAMTEDDEATVKRFFKEETHIRLQP
EEEEECCCHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEECC
ENPTMEPIILQNVTILGKVIGVFRTVH
CCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKLSKRQLDILRFIKEEVKRKGYPPSVREIGEAVGLASSSTVHGHLARLETKGLIRRDP
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCC
TKPRAIEILDTEEEIHIPKNQVVNVPVIGKVTAGTPITAVENIEEYFPLPERLAPPDEHV
CCCCEEEEEECCCCEECCCCCEEECCEEEEECCCCCCHHHHHHHHHCCCHHHCCCCCCCE
FMLEIMGESMIDAGILDQDYVIVKQQNTANNGDIVVAMTEDDEATVKRFFKEETHIRLQP
EEEEECCCHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEECC
ENPTMEPIILQNVTILGKVIGVFRTVH
CCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA