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The map label for this gene is cwlC [H]

Identifier: 52785731

GI number: 52785731

Start: 1928606

End: 1929367

Strand: Reverse

Name: cwlC [H]

Synonym: BLi01974

Alternate gene names: 52785731

Gene position: 1929367-1928606 (Counterclockwise)

Preceding gene: 52785732

Following gene: 52785726

Centisome position: 45.69

GC content: 48.03

Gene sequence:

>762_bases
GTGGTAAAAATTTTTATCGATCCCGGCCATGGCGGGACTGATCCAGGCGCTTCAGCCAATGGTTTGCAAGAAAAACAGTT
AACATTGCAAATCGCCTTGGCTTTAAGAAACATTCTCCTGAATGAATATCAAAATGTTACAGTCCAGCTGAGCCGGACAA
GCGACCAAACCGTCAGTCTGACGCAACGCACGAACGCCGCGAACAGCTGGGGGGCGGACTACTTTCTATCGATTCACATC
AATGCCGGGGGCGGGACAGGTTTCGAGGATTACATTTATCCCGGCGTCGGCGCACCTACCACCACCTATCGGGATATCAT
GCATGAAGAGATTTTAAAAGTGGTTGATTTCAGGGATCGCGGCAAGAAAACAGCCAATTTTCACGTGCTAAGGGAAACAG
CAATGCCAGCACTGCTTACGGAAAATGGCTTTGTAGACAATACAAGCGACGCCGAGAAACTGAAGTCATCCGCGTTTATT
CAATCCATTGCGAGAGGCCACGCCAACGGACTGGCAAGGGCATTTAACCTTTCAAAAAAATCCACAGCCCTTTTTAAAGT
GCAAATCGGCGCTTTCAGGGACAAAGCCAACGCCGATTCACTTGCCGCTCAAGCGAAAGCAAAAGGGTTTGATGCCATTG
TAATTGACAGGGACAGCCTTTACAAAGTGCAAATTGGCGCCTTTTCTTCGAAAGAGAATGCAGAAGCGCTTGTTCAGCAA
GCCAAAAACGCCGGATTTGATGCTTTCATTTACCAGGAATAG

Upstream 100 bases:

>100_bases
CTATAATAAGCTGAATATTTAAAAAATTCCGTTATCAGGTGACAATCGTACAACCCTTCCCATACATCAGTTCATAGACT
GTTTATTGAGGTGATGTCTT

Downstream 100 bases:

>100_bases
GGACAATCATCAAGAAAAGCCCGCGATCTGTCTCGTATAGATCGCAGGCTTTTTTTATGCTTTTGCAGCTTTACTTTGAA
CCGCCGGCTTTTGATTCGAT

Product: CwlC

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MVKIFIDPGHGGTDPGASANGLQEKQLTLQIALALRNILLNEYQNVTVQLSRTSDQTVSLTQRTNAANSWGADYFLSIHI
NAGGGTGFEDYIYPGVGAPTTTYRDIMHEEILKVVDFRDRGKKTANFHVLRETAMPALLTENGFVDNTSDAEKLKSSAFI
QSIARGHANGLARAFNLSKKSTALFKVQIGAFRDKANADSLAAQAKAKGFDAIVIDRDSLYKVQIGAFSSKENAEALVQQ
AKNAGFDAFIYQE

Sequences:

>Translated_253_residues
MVKIFIDPGHGGTDPGASANGLQEKQLTLQIALALRNILLNEYQNVTVQLSRTSDQTVSLTQRTNAANSWGADYFLSIHI
NAGGGTGFEDYIYPGVGAPTTTYRDIMHEEILKVVDFRDRGKKTANFHVLRETAMPALLTENGFVDNTSDAEKLKSSAFI
QSIARGHANGLARAFNLSKKSTALFKVQIGAFRDKANADSLAAQAKAKGFDAIVIDRDSLYKVQIGAFSSKENAEALVQQ
AKNAGFDAFIYQE
>Mature_253_residues
MVKIFIDPGHGGTDPGASANGLQEKQLTLQIALALRNILLNEYQNVTVQLSRTSDQTVSLTQRTNAANSWGADYFLSIHI
NAGGGTGFEDYIYPGVGAPTTTYRDIMHEEILKVVDFRDRGKKTANFHVLRETAMPALLTENGFVDNTSDAEKLKSSAFI
QSIARGHANGLARAFNLSKKSTALFKVQIGAFRDKANADSLAAQAKAKGFDAIVIDRDSLYKVQIGAFSSKENAEALVQQ
AKNAGFDAFIYQE

Specific function: Hydrolyzes the cell wall of M.luteus more efficiently than that of B.licheniformis and B.subtilis. The C-terminal region, including the repeats, determines substrate specificity [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Probable). Note=Accumulates in cells as inclusion bodies. May be secreted by a mechanism different from the normal export system [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=221, Percent_Identity=27.1493212669683, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR007730 [H]

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 27481; Mature: 27481

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIFIDPGHGGTDPGASANGLQEKQLTLQIALALRNILLNEYQNVTVQLSRTSDQTVSL
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEE
TQRTNAANSWGADYFLSIHINAGGGTGFEDYIYPGVGAPTTTYRDIMHEEILKVVDFRDR
EECCCCCCCCCCEEEEEEEEECCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GKKTANFHVLRETAMPALLTENGFVDNTSDAEKLKSSAFIQSIARGHANGLARAFNLSKK
CCCCCCEEEEHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
STALFKVQIGAFRDKANADSLAAQAKAKGFDAIVIDRDSLYKVQIGAFSSKENAEALVQQ
CEEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHH
AKNAGFDAFIYQE
HHCCCCEEEEECC
>Mature Secondary Structure
MVKIFIDPGHGGTDPGASANGLQEKQLTLQIALALRNILLNEYQNVTVQLSRTSDQTVSL
CEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEE
TQRTNAANSWGADYFLSIHINAGGGTGFEDYIYPGVGAPTTTYRDIMHEEILKVVDFRDR
EECCCCCCCCCCEEEEEEEEECCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GKKTANFHVLRETAMPALLTENGFVDNTSDAEKLKSSAFIQSIARGHANGLARAFNLSKK
CCCCCCEEEEHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
STALFKVQIGAFRDKANADSLAAQAKAKGFDAIVIDRDSLYKVQIGAFSSKENAEALVQQ
CEEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHH
AKNAGFDAFIYQE
HHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1495475 [H]