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The map label for this gene is pit [H]

Identifier: 52785130

GI number: 52785130

Start: 1352706

End: 1353704

Strand: Reverse

Name: pit [H]

Synonym: BLi01362

Alternate gene names: 52785130

Gene position: 1353704-1352706 (Counterclockwise)

Preceding gene: 52785131

Following gene: 52785129

Centisome position: 32.06

GC content: 48.15

Gene sequence:

>999_bases
ATGGATCTTTTATTTGTATTAACCATATTGATTGTCATCTTTGCTTTGGCATTTGACTTTATCAACGGCTTTCATGATAC
GGCAAATGCGATCGCCACCTCGGTATCGACAAAAGCGTTAAAACCAAGGCATGCCATTATTATGGCCGCTGTCATGAACT
TCGCCGGCGCCATGACGTTCACAGGCGTAGCCAAAACAATTACAAGCGACATCGCCGACCCTTTCAAGCTTGAAAACGGA
TCGGTCGTGATCCTTGCCGCTCTTATCGCCGGCATTACATGGAACCTAGTGACCTGGTATTTCGGGATTCCCAGCAGCTC
ATCCCATGCGATCATCGGAGCGATTGCCGGAGCTGTCATCGCATCGGAAGGATTTGCCGCGATTAAATACCACGGATTTA
TTAAAATTATTGAAGCACTTATATTATCGCCCATTTTGGCGTTTGTTTTAGGATATTTGATCTACAGCATCGTCAAAGTC
ATTTTTAAGAACAGCAATCTGGCAAAAACGAATAAGCGGTTTCGGCGCGTTCAAATCGCGACAGCTGCCATTCAGTCCTA
TACGCACGGCACGAACGATGCACAGAAAGCGATGGGTATCATTACGATGGCTTTAATCGCGGGTAACTTGCACAGTACAA
CCGACATCCCGTTCTGGGTGCAGTTTGCATGCGCCATGGCCATGGGACTCGGAACATCTGTCGGCGGCTGGAAAATCATC
AAAACCGTCGGCGGAAAAATCATGAAGATCCGTCCCGTCAACGGTGTCTCTGCAGATTTGACGGGAGCCCTCATCATCTT
TGGCGCGACGATCGTCCATTTGCCTGTCAGCACGACACACGTCATTTCTTCATCCATTTTAGGCGTGGGCGCTTCCCACC
GGGTGAAAGGCGTAAACTGGGGCACCGCAAAGCGGATGCTGATCACTTGGGTCATTACCCTTCCCATTTCGGCAACCATC
GGTGCATTTGCCTATTTCATACTTGATTTATTCTTTTAA

Upstream 100 bases:

>100_bases
AGAAATCTATGAGACGCTTGAAGAAATCGCAGATTCATGCCAAAGTGTAGCAAACAATTTAGAAACCATCATAATGAAAA
ATGCGTAACGAGGGATTATA

Downstream 100 bases:

>100_bases
TCAGCACACTCCCGTCCGAATCTAGGACGGGAGTTTTCATGTTTAAAAGAAAGCCGGCATAACAATAGAAATAGGAACTG
CACTATTTCTCGGGAAGAGG

Product: Pit

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MDLLFVLTILIVIFALAFDFINGFHDTANAIATSVSTKALKPRHAIIMAAVMNFAGAMTFTGVAKTITSDIADPFKLENG
SVVILAALIAGITWNLVTWYFGIPSSSSHAIIGAIAGAVIASEGFAAIKYHGFIKIIEALILSPILAFVLGYLIYSIVKV
IFKNSNLAKTNKRFRRVQIATAAIQSYTHGTNDAQKAMGIITMALIAGNLHSTTDIPFWVQFACAMAMGLGTSVGGWKII
KTVGGKIMKIRPVNGVSADLTGALIIFGATIVHLPVSTTHVISSSILGVGASHRVKGVNWGTAKRMLITWVITLPISATI
GAFAYFILDLFF

Sequences:

>Translated_332_residues
MDLLFVLTILIVIFALAFDFINGFHDTANAIATSVSTKALKPRHAIIMAAVMNFAGAMTFTGVAKTITSDIADPFKLENG
SVVILAALIAGITWNLVTWYFGIPSSSSHAIIGAIAGAVIASEGFAAIKYHGFIKIIEALILSPILAFVLGYLIYSIVKV
IFKNSNLAKTNKRFRRVQIATAAIQSYTHGTNDAQKAMGIITMALIAGNLHSTTDIPFWVQFACAMAMGLGTSVGGWKII
KTVGGKIMKIRPVNGVSADLTGALIIFGATIVHLPVSTTHVISSSILGVGASHRVKGVNWGTAKRMLITWVITLPISATI
GAFAYFILDLFF
>Mature_332_residues
MDLLFVLTILIVIFALAFDFINGFHDTANAIATSVSTKALKPRHAIIMAAVMNFAGAMTFTGVAKTITSDIADPFKLENG
SVVILAALIAGITWNLVTWYFGIPSSSSHAIIGAIAGAVIASEGFAAIKYHGFIKIIEALILSPILAFVLGYLIYSIVKV
IFKNSNLAKTNKRFRRVQIATAAIQSYTHGTNDAQKAMGIITMALIAGNLHSTTDIPFWVQFACAMAMGLGTSVGGWKII
KTVGGKIMKIRPVNGVSADLTGALIIFGATIVHLPVSTTHVISSSILGVGASHRVKGVNWGTAKRMLITWVITLPISATI
GAFAYFILDLFF

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=165, Percent_Identity=32.7272727272727, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI5803173, Length=152, Percent_Identity=30.2631578947368, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1789360, Length=221, Percent_Identity=31.6742081447964, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=31.7972350230415, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=30.8641975308642, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17539280, Length=166, Percent_Identity=28.3132530120482, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71981576, Length=161, Percent_Identity=31.6770186335404, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32566716, Length=164, Percent_Identity=28.0487804878049, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17536725, Length=172, Percent_Identity=26.7441860465116, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319773, Length=151, Percent_Identity=30.4635761589404, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35318; Mature: 35318

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLFVLTILIVIFALAFDFINGFHDTANAIATSVSTKALKPRHAIIMAAVMNFAGAMTF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TGVAKTITSDIADPFKLENGSVVILAALIAGITWNLVTWYFGIPSSSSHAIIGAIAGAVI
HHHHHHHHHHCCCCEEECCCCEEHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHH
ASEGFAAIKYHGFIKIIEALILSPILAFVLGYLIYSIVKVIFKNSNLAKTNKRFRRVQIA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TAAIQSYTHGTNDAQKAMGIITMALIAGNLHSTTDIPFWVQFACAMAMGLGTSVGGWKII
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
KTVGGKIMKIRPVNGVSADLTGALIIFGATIVHLPVSTTHVISSSILGVGASHRVKGVNW
HHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCCCCCCCCCCC
GTAKRMLITWVITLPISATIGAFAYFILDLFF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDLLFVLTILIVIFALAFDFINGFHDTANAIATSVSTKALKPRHAIIMAAVMNFAGAMTF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TGVAKTITSDIADPFKLENGSVVILAALIAGITWNLVTWYFGIPSSSSHAIIGAIAGAVI
HHHHHHHHHHCCCCEEECCCCEEHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHH
ASEGFAAIKYHGFIKIIEALILSPILAFVLGYLIYSIVKVIFKNSNLAKTNKRFRRVQIA
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TAAIQSYTHGTNDAQKAMGIITMALIAGNLHSTTDIPFWVQFACAMAMGLGTSVGGWKII
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHH
KTVGGKIMKIRPVNGVSADLTGALIIFGATIVHLPVSTTHVISSSILGVGASHRVKGVNW
HHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHEECCCHHHHHHHHHHCCCCCCCCCCCCC
GTAKRMLITWVITLPISATIGAFAYFILDLFF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]