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The map label for this gene is ywfK [H]

Identifier: 52785069

GI number: 52785069

Start: 1302214

End: 1303104

Strand: Reverse

Name: ywfK [H]

Synonym: BLi01300

Alternate gene names: 52785069

Gene position: 1303104-1302214 (Counterclockwise)

Preceding gene: 52785072

Following gene: 52785068

Centisome position: 30.86

GC content: 44.67

Gene sequence:

>891_bases
GTGCATTATGATGCGCTGAAGACATTCGTTACCCTTGCAGAAGTCAAAAATTTCACGAAAACGGCCGAGCTTCTTCTCAT
GTCGCAGCCGAGCGTAAGCATGCACATTAAAAATCTTGAAAAAGAATTCCAGACGAAATTATTTGAACGCTCTCCGAAAT
ATTTAAAAATCACGCCGACCGGTGAAATTTTATACGACCGCGCCAAACAAATGATCACCATCTATGAACAAACGAGGCAG
GACATTCTCGATCACCACAACTCGATCAAAGGCGAGCTGAAAATCGGCGCAAGCTTCACAATCGGTGAATATATTTTGCC
TTCGCTCCTCTTCGACCTTCAAAAGGAATATCCTGAACTCGAGCTCAAGGCCGTCATTGGAAACACGGAAGAAATCGTGC
AATCCGTCCGCCTGTACAAAGTGGACATCGGCTTGATCGAAGGACAGACAAATGAAAAAGAGCTTTCTGTACACGCCTTT
ATGCAGGACGAATTATTCATCGCTGCTTCAAGCGATCATCCGCTTGCGGCCAAACAGGACGTGACAATCTCCGATCTGCA
AAATCAAACGTGGGTAACGCGCGAAGTCGGCTCAGGCACACGCGAATACTTAAACCATGTCATCCGTTCAAACGGTTTAA
AAGTGAAATCGTTTCTGACGATCAGCAGCAACCAGGGGATCAAAGAAACGCTCATGACGGGCATCGGACTGTCTCTCCTG
TCTGGAAGCGTCATTGAAAGAGATGTACAGCACGGAAACCTTTCCGTTATCCGGCTGAAAAATCAAACCTTTAACAGGAC
GCTTTCCTATATATATTCACCTGTCATGCAGGACAAGAAAAATGTGAAAACCTTTATTAACGCTTTGAACAAAAGAACAT
CTTCGAAATAA

Upstream 100 bases:

>100_bases
AAAAAAGATTCTAATTAAAAGTTTACTGGAATACTCAACATTAGTAAAATATATATTAATTATCACAGCTATTAAGATTT
TTAATAAAGGAGAGATTCGT

Downstream 100 bases:

>100_bases
TGAGTCGTTTTTCCTGCCGCTTCACAAAATGTTTTTTTTCGTATGTCCAATGTGTGAATCATCATTGGAGCCAATTGACT
GCCGCCCAACAATCCCATAT

Product: YwfK

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MHYDALKTFVTLAEVKNFTKTAELLLMSQPSVSMHIKNLEKEFQTKLFERSPKYLKITPTGEILYDRAKQMITIYEQTRQ
DILDHHNSIKGELKIGASFTIGEYILPSLLFDLQKEYPELELKAVIGNTEEIVQSVRLYKVDIGLIEGQTNEKELSVHAF
MQDELFIAASSDHPLAAKQDVTISDLQNQTWVTREVGSGTREYLNHVIRSNGLKVKSFLTISSNQGIKETLMTGIGLSLL
SGSVIERDVQHGNLSVIRLKNQTFNRTLSYIYSPVMQDKKNVKTFINALNKRTSSK

Sequences:

>Translated_296_residues
MHYDALKTFVTLAEVKNFTKTAELLLMSQPSVSMHIKNLEKEFQTKLFERSPKYLKITPTGEILYDRAKQMITIYEQTRQ
DILDHHNSIKGELKIGASFTIGEYILPSLLFDLQKEYPELELKAVIGNTEEIVQSVRLYKVDIGLIEGQTNEKELSVHAF
MQDELFIAASSDHPLAAKQDVTISDLQNQTWVTREVGSGTREYLNHVIRSNGLKVKSFLTISSNQGIKETLMTGIGLSLL
SGSVIERDVQHGNLSVIRLKNQTFNRTLSYIYSPVMQDKKNVKTFINALNKRTSSK
>Mature_296_residues
MHYDALKTFVTLAEVKNFTKTAELLLMSQPSVSMHIKNLEKEFQTKLFERSPKYLKITPTGEILYDRAKQMITIYEQTRQ
DILDHHNSIKGELKIGASFTIGEYILPSLLFDLQKEYPELELKAVIGNTEEIVQSVRLYKVDIGLIEGQTNEKELSVHAF
MQDELFIAASSDHPLAAKQDVTISDLQNQTWVTREVGSGTREYLNHVIRSNGLKVKSFLTISSNQGIKETLMTGIGLSLL
SGSVIERDVQHGNLSVIRLKNQTFNRTLSYIYSPVMQDKKNVKTFINALNKRTSSK

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=271, Percent_Identity=32.8413284132841, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI157672245, Length=284, Percent_Identity=26.056338028169, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788748, Length=288, Percent_Identity=24.3055555555556, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI145693105, Length=270, Percent_Identity=25.1851851851852, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790399, Length=288, Percent_Identity=23.2638888888889, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1787530, Length=270, Percent_Identity=25.5555555555556, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787879, Length=247, Percent_Identity=25.5060728744939, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788296, Length=227, Percent_Identity=25.1101321585903, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788887, Length=276, Percent_Identity=26.8115942028986, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788297, Length=250, Percent_Identity=23.6, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1790208, Length=245, Percent_Identity=26.530612244898, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87082132, Length=257, Percent_Identity=23.7354085603113, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789639, Length=122, Percent_Identity=31.9672131147541, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87081904, Length=299, Percent_Identity=21.4046822742475, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787601, Length=189, Percent_Identity=21.1640211640212, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=244, Percent_Identity=25, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790262, Length=242, Percent_Identity=22.7272727272727, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33615; Mature: 33615

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYDALKTFVTLAEVKNFTKTAELLLMSQPSVSMHIKNLEKEFQTKLFERSPKYLKITPT
CCHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCC
GEILYDRAKQMITIYEQTRQDILDHHNSIKGELKIGASFTIGEYILPSLLFDLQKEYPEL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCE
ELKAVIGNTEEIVQSVRLYKVDIGLIEGQTNEKELSVHAFMQDELFIAASSDHPLAAKQD
EEEEECCCHHHHHHHHHHEEEEEEEEECCCCCHHEEEEEEEECCEEEEECCCCCCCCCCC
VTISDLQNQTWVTREVGSGTREYLNHVIRSNGLKVKSFLTISSNQGIKETLMTGIGLSLL
CCHHHCCCCCEEEHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
SGSVIERDVQHGNLSVIRLKNQTFNRTLSYIYSPVMQDKKNVKTFINALNKRTSSK
CCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHYDALKTFVTLAEVKNFTKTAELLLMSQPSVSMHIKNLEKEFQTKLFERSPKYLKITPT
CCHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCC
GEILYDRAKQMITIYEQTRQDILDHHNSIKGELKIGASFTIGEYILPSLLFDLQKEYPEL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCE
ELKAVIGNTEEIVQSVRLYKVDIGLIEGQTNEKELSVHAFMQDELFIAASSDHPLAAKQD
EEEEECCCHHHHHHHHHHEEEEEEEEECCCCCHHEEEEEEEECCEEEEECCCCCCCCCCC
VTISDLQNQTWVTREVGSGTREYLNHVIRSNGLKVKSFLTISSNQGIKETLMTGIGLSLL
CCHHHCCCCCEEEHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
SGSVIERDVQHGNLSVIRLKNQTFNRTLSYIYSPVMQDKKNVKTFINALNKRTSSK
CCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]