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The map label for this gene is citS [H]

Identifier: 52785016

GI number: 52785016

Start: 1257028

End: 1258623

Strand: Reverse

Name: citS [H]

Synonym: BLi01245

Alternate gene names: 52785016

Gene position: 1258623-1257028 (Counterclockwise)

Preceding gene: 52785019

Following gene: 52785015

Centisome position: 29.81

GC content: 46.18

Gene sequence:

>1596_bases
TTGAGAAAAGGAGTTTTTGACATCAAACGGAAGCTGACCACCAAGCTCACATTGCTGATTCTTACCCTGCTTTTGCTCTT
GCTTTCTGTCATGAGCGGCCTCTATTTAATTATTTTAGAAAATTCTTTAAAAACACAAATCCAGGCCAGAGCGCTCGATA
TGGCGAAAACGCTCGCCCACATGCCTTCCGTTCAGAAAGCGTTTGAAACGGACGATCCGTCTCGAACCCTTCAGCCAATT
GCAGAAAATGCGAGAAAACAGTCCGGTGCGCAATACATCGTGATCGGCTCGGCGGATGCGATTCGCTATGCTCATCCGGA
CCCTGGAAAAATCGGCAAACCAATGGTCGGCGGAGACAATCGGCGCGCCCTTGAAAAAGGTGAAGCATACGTTTCGGAAG
CCGTCGGCACGCTTGGCCCGGCCATCAGGGGCAAAGCGCCGATCCATAATGAAAACGGCGAAATCGTCGGCATCATCTCA
GTCGGTTTTTTAATAGAAAATATTCAGGACATCGTCTATCAATATCAGTCAAAATTCATATTTTTATTGGGATTTGTCGT
GCTGATGGGGATTTTGGGAGCCGTCTATATTTCCCGCAATGTCAAAAAGTCCATTTTCGGCCTTGAGCCTTATGAAATTA
ATCAATTATATACTGAAAAGCAGGCGATTACAGAATCTGTGCACGAGGCCTTGATCGCCATCAATAAACATGAAGAAATC
ACGCTTTTGAATAGAAAAGCGTGTGAGCTGCTTCGTGTCGAAAGCGAAGATCAGTGGCTCGGCAAACCGATTTCCGAGCT
TCTTCCGGATACGCTGCTCCCCGAAGTGCTGAAAACGAAAACGGCGCAAAAAGATCAGGAATTAACGATCGGAAACGAAG
AATTAATCGTCAACAGAGTGCCGATTTTCGAAAACGAAAAAATCCGCGGCGCCGTTGCGAGCTTCAGGAGAAAGACGGAT
ATTGACGAACTCTCCAAAAAGCTGGCGCAAACTTCGCAATACGCGGAAGGTCTGAGAGCCCAGACCCACGAATATTCAAA
CAAGCTGAATACAATCGCGGGACTGATTCAGCTGGAAAGCTATCCTGAAGCACTCGAGTTGATCCAAAAAGAGAATAAAA
GCCATCAGGATATGCTGCAATTTCTCATCCATTCGATTCCAGATCCGCTTCTTTCCGGGTTGCTGCTGGGTAAGGTCAAC
CGTGCCAAAGAGCTGCACATCAACCTGGAAATTGATGAAAGCTCAAAATTGAACGCTTTGCCGGACGAAATGGATAAGGG
AAAATTCATTACGATTTTAGGAAATTTGATTGAGAATGCCTTTGACGCCGTTTCCCACAAAAAAGACCGCAGCATCCTTG
TCTTTTTGTCAGACGGTGCAGATCACATATTAATTGAAGTCGAAGACTCGGGAGACGGGATTCCAAAGGGGGAACAGGAG
CGGATCTTTCAAAAGTTTTATTCGACAAAAACGGCAAACCACCGCGGCCTCGGCCTCTTTTTAGTTAAAGAAGCCGTCAA
TGCGTTAAACGGGCAAATTAAAGCGGACAAAAGCGACCTTGGCGGAGCATTGTTTACTGTCGTGATACCAAAATAG

Upstream 100 bases:

>100_bases
TTTGATCTAGTATTCAACATTGGCGGGAGCTTTCCTTTATTTGAAAACGCTTCAAATCCAGGCCGATTTTATATAAAGTA
GTATTATAGAACTCGAAATT

Downstream 100 bases:

>100_bases
AGAAACGCTGGGGGACTGACCTATGAAAGCATTACAAGTATTTATCGTAGAGGATGACATCAGAAACGCTGACATCCACC
AAAAACTGATCAGCAAAATT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MRKGVFDIKRKLTTKLTLLILTLLLLLLSVMSGLYLIILENSLKTQIQARALDMAKTLAHMPSVQKAFETDDPSRTLQPI
AENARKQSGAQYIVIGSADAIRYAHPDPGKIGKPMVGGDNRRALEKGEAYVSEAVGTLGPAIRGKAPIHNENGEIVGIIS
VGFLIENIQDIVYQYQSKFIFLLGFVVLMGILGAVYISRNVKKSIFGLEPYEINQLYTEKQAITESVHEALIAINKHEEI
TLLNRKACELLRVESEDQWLGKPISELLPDTLLPEVLKTKTAQKDQELTIGNEELIVNRVPIFENEKIRGAVASFRRKTD
IDELSKKLAQTSQYAEGLRAQTHEYSNKLNTIAGLIQLESYPEALELIQKENKSHQDMLQFLIHSIPDPLLSGLLLGKVN
RAKELHINLEIDESSKLNALPDEMDKGKFITILGNLIENAFDAVSHKKDRSILVFLSDGADHILIEVEDSGDGIPKGEQE
RIFQKFYSTKTANHRGLGLFLVKEAVNALNGQIKADKSDLGGALFTVVIPK

Sequences:

>Translated_531_residues
MRKGVFDIKRKLTTKLTLLILTLLLLLLSVMSGLYLIILENSLKTQIQARALDMAKTLAHMPSVQKAFETDDPSRTLQPI
AENARKQSGAQYIVIGSADAIRYAHPDPGKIGKPMVGGDNRRALEKGEAYVSEAVGTLGPAIRGKAPIHNENGEIVGIIS
VGFLIENIQDIVYQYQSKFIFLLGFVVLMGILGAVYISRNVKKSIFGLEPYEINQLYTEKQAITESVHEALIAINKHEEI
TLLNRKACELLRVESEDQWLGKPISELLPDTLLPEVLKTKTAQKDQELTIGNEELIVNRVPIFENEKIRGAVASFRRKTD
IDELSKKLAQTSQYAEGLRAQTHEYSNKLNTIAGLIQLESYPEALELIQKENKSHQDMLQFLIHSIPDPLLSGLLLGKVN
RAKELHINLEIDESSKLNALPDEMDKGKFITILGNLIENAFDAVSHKKDRSILVFLSDGADHILIEVEDSGDGIPKGEQE
RIFQKFYSTKTANHRGLGLFLVKEAVNALNGQIKADKSDLGGALFTVVIPK
>Mature_531_residues
MRKGVFDIKRKLTTKLTLLILTLLLLLLSVMSGLYLIILENSLKTQIQARALDMAKTLAHMPSVQKAFETDDPSRTLQPI
AENARKQSGAQYIVIGSADAIRYAHPDPGKIGKPMVGGDNRRALEKGEAYVSEAVGTLGPAIRGKAPIHNENGEIVGIIS
VGFLIENIQDIVYQYQSKFIFLLGFVVLMGILGAVYISRNVKKSIFGLEPYEINQLYTEKQAITESVHEALIAINKHEEI
TLLNRKACELLRVESEDQWLGKPISELLPDTLLPEVLKTKTAQKDQELTIGNEELIVNRVPIFENEKIRGAVASFRRKTD
IDELSKKLAQTSQYAEGLRAQTHEYSNKLNTIAGLIQLESYPEALELIQKENKSHQDMLQFLIHSIPDPLLSGLLLGKVN
RAKELHINLEIDESSKLNALPDEMDKGKFITILGNLIENAFDAVSHKKDRSILVFLSDGADHILIEVEDSGDGIPKGEQE
RIFQKFYSTKTANHRGLGLFLVKEAVNALNGQIKADKSDLGGALFTVVIPK

Specific function: Member of the two-component regulatory system CitT/CitS. Functions probably as a membrane-associated protein kinase that phosphorylates CitT in response to environmental citrate or Mg(2+)-citrate complex [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786837, Length=527, Percent_Identity=34.3453510436433, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1790567, Length=522, Percent_Identity=33.3333333333333, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1788549, Length=439, Percent_Identity=27.1070615034169, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58985; Mature: 58985

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKGVFDIKRKLTTKLTLLILTLLLLLLSVMSGLYLIILENSLKTQIQARALDMAKTLAH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHH
MPSVQKAFETDDPSRTLQPIAENARKQSGAQYIVIGSADAIRYAHPDPGKIGKPMVGGDN
CCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCC
RRALEKGEAYVSEAVGTLGPAIRGKAPIHNENGEIVGIISVGFLIENIQDIVYQYQSKFI
HHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
FLLGFVVLMGILGAVYISRNVKKSIFGLEPYEINQLYTEKQAITESVHEALIAINKHEEI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TLLNRKACELLRVESEDQWLGKPISELLPDTLLPEVLKTKTAQKDQELTIGNEELIVNRV
EEHHHHHHHHHHCCCCCHHHCCCHHHHCCHHHHHHHHHHHHCCCCCCEECCCCEEEEECC
PIFENEKIRGAVASFRRKTDIDELSKKLAQTSQYAEGLRAQTHEYSNKLNTIAGLIQLES
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YPEALELIQKENKSHQDMLQFLIHSIPDPLLSGLLLGKVNRAKELHINLEIDESSKLNAL
CHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCC
PDEMDKGKFITILGNLIENAFDAVSHKKDRSILVFLSDGADHILIEVEDSGDGIPKGEQE
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCHHH
RIFQKFYSTKTANHRGLGLFLVKEAVNALNGQIKADKSDLGGALFTVVIPK
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEECCHHHCCCEEEEEEECC
>Mature Secondary Structure
MRKGVFDIKRKLTTKLTLLILTLLLLLLSVMSGLYLIILENSLKTQIQARALDMAKTLAH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHH
MPSVQKAFETDDPSRTLQPIAENARKQSGAQYIVIGSADAIRYAHPDPGKIGKPMVGGDN
CCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCC
RRALEKGEAYVSEAVGTLGPAIRGKAPIHNENGEIVGIISVGFLIENIQDIVYQYQSKFI
HHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
FLLGFVVLMGILGAVYISRNVKKSIFGLEPYEINQLYTEKQAITESVHEALIAINKHEEI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
TLLNRKACELLRVESEDQWLGKPISELLPDTLLPEVLKTKTAQKDQELTIGNEELIVNRV
EEHHHHHHHHHHCCCCCHHHCCCHHHHCCHHHHHHHHHHHHCCCCCCEECCCCEEEEECC
PIFENEKIRGAVASFRRKTDIDELSKKLAQTSQYAEGLRAQTHEYSNKLNTIAGLIQLES
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YPEALELIQKENKSHQDMLQFLIHSIPDPLLSGLLLGKVNRAKELHINLEIDESSKLNAL
CHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCC
PDEMDKGKFITILGNLIENAFDAVSHKKDRSILVFLSDGADHILIEVEDSGDGIPKGEQE
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCHHH
RIFQKFYSTKTANHRGLGLFLVKEAVNALNGQIKADKSDLGGALFTVVIPK
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEECCHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]