Click here to switch to the map view.

The map label for this gene is cwlM [H]

Identifier: 52784803

GI number: 52784803

Start: 1032705

End: 1033382

Strand: Direct

Name: cwlM [H]

Synonym: BLi01031

Alternate gene names: 52784803

Gene position: 1032705-1033382 (Clockwise)

Preceding gene: 52784802

Following gene: 52784807

Centisome position: 24.46

GC content: 50.29

Gene sequence:

>678_bases
ATGAAGATTTATTTGGATCCGGGACACGGCGGAAGTGATCCGGGAGCATCGGGAAACGGTTTGCTGGAAAAGGACGTGAC
GCTTGCCATTGCTCTCAAAATCAATGACATTTTATTAAAAGAGTATGCAGGCGCAGAGACGAGGCTGAGCAGAACGACAG
ACAAAACGGTTACGCTTCAGGAGCGCACAGACGATGCGAATTCGTGGGGGGCCGATTTGTTTGTCTCCATTCATACAAAC
GCTTTTGACGGCACGGCGTACGGGTATGAGGATTACATCTACAGCGGCCTGTCCGATGAATCAGTTACCGCTCTTTATCA
ACGGATCATTCACGGCGAAGTAATCAAGCAGAACGGACTCAGGGACAGGGGAATGAAAAAGGCGAACTTTCATGTTCTTC
GGGAAACAGCGATGCACGCCGTTTTAACGGAGAACGGCTTTATCGATCACCCCGAAGATTCGGCAAAAATGAAAAGCGCC
GCCTGGATCGAACAGACTGCGCGGGGACATGCAAATGGCATCGCGCTCTGCCTCGGCCTGACGCAGCGCGAATTCCCCTT
GTATAAAGTGACGATGGACGGGGGGCAAATCGGGGCTTACCAAGAAAAAGACGACGTTTTGAAAGTTATTTCTGCGAATT
GGGACAGCTTTCAAAAAGCGGCTATTGAAAAAGGGTAG

Upstream 100 bases:

>100_bases
GAAGACATGATGATCGGAACTTGGCTTGGATTGTCATCAGTTGGATTTTACTCCGGTTCAAAAAACGTCTTGAAACCATT
ATCAAAGGAGGAAATTGAAC

Downstream 100 bases:

>100_bases
AAAGGGAAGAATCCATTTTGAAGCATTCAAAATGGATTCTTGACATTTAATGAGTCCGCATCTTTTTTCATTTGGGCGTA
ATGTTCCTTTCTGAACGCTC

Product: hypothetical protein

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MKIYLDPGHGGSDPGASGNGLLEKDVTLAIALKINDILLKEYAGAETRLSRTTDKTVTLQERTDDANSWGADLFVSIHTN
AFDGTAYGYEDYIYSGLSDESVTALYQRIIHGEVIKQNGLRDRGMKKANFHVLRETAMHAVLTENGFIDHPEDSAKMKSA
AWIEQTARGHANGIALCLGLTQREFPLYKVTMDGGQIGAYQEKDDVLKVISANWDSFQKAAIEKG

Sequences:

>Translated_225_residues
MKIYLDPGHGGSDPGASGNGLLEKDVTLAIALKINDILLKEYAGAETRLSRTTDKTVTLQERTDDANSWGADLFVSIHTN
AFDGTAYGYEDYIYSGLSDESVTALYQRIIHGEVIKQNGLRDRGMKKANFHVLRETAMHAVLTENGFIDHPEDSAKMKSA
AWIEQTARGHANGIALCLGLTQREFPLYKVTMDGGQIGAYQEKDDVLKVISANWDSFQKAAIEKG
>Mature_225_residues
MKIYLDPGHGGSDPGASGNGLLEKDVTLAIALKINDILLKEYAGAETRLSRTTDKTVTLQERTDDANSWGADLFVSIHTN
AFDGTAYGYEDYIYSGLSDESVTALYQRIIHGEVIKQNGLRDRGMKKANFHVLRETAMHAVLTENGFIDHPEDSAKMKSA
AWIEQTARGHANGIALCLGLTQREFPLYKVTMDGGQIGAYQEKDDVLKVISANWDSFQKAAIEKG

Specific function: Hydrolyzes the cell wall of M.luteus more efficiently than that of B.licheniformis and B.subtilis. The C-terminal region, including the repeats, determines substrate specificity [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Probable). Note=Accumulates in cells as inclusion bodies. May be secreted by a mechanism different from the normal export system [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=216, Percent_Identity=30.5555555555556, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87082163, Length=215, Percent_Identity=28.8372093023256, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR007730 [H]

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 24699; Mature: 24699

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLDPGHGGSDPGASGNGLLEKDVTLAIALKINDILLKEYAGAETRLSRTTDKTVTLQ
CEEEECCCCCCCCCCCCCCCEEECCEEEEEEEEEHHHHHHHHCCCCHHHHCCCCCEEEEE
ERTDDANSWGADLFVSIHTNAFDGTAYGYEDYIYSGLSDESVTALYQRIIHGEVIKQNGL
ECCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
RDRGMKKANFHVLRETAMHAVLTENGFIDHPEDSAKMKSAAWIEQTARGHANGIALCLGL
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEC
TQREFPLYKVTMDGGQIGAYQEKDDVLKVISANWDSFQKAAIEKG
CCCCCCEEEEEECCCEECCCCCHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MKIYLDPGHGGSDPGASGNGLLEKDVTLAIALKINDILLKEYAGAETRLSRTTDKTVTLQ
CEEEECCCCCCCCCCCCCCCEEECCEEEEEEEEEHHHHHHHHCCCCHHHHCCCCCEEEEE
ERTDDANSWGADLFVSIHTNAFDGTAYGYEDYIYSGLSDESVTALYQRIIHGEVIKQNGL
ECCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
RDRGMKKANFHVLRETAMHAVLTENGFIDHPEDSAKMKSAAWIEQTARGHANGIALCLGL
HHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEC
TQREFPLYKVTMDGGQIGAYQEKDDVLKVISANWDSFQKAAIEKG
CCCCCCEEEEEECCCEECCCCCHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1495475 [H]