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The map label for this gene is hmp [H]

Identifier: 52784778

GI number: 52784778

Start: 1007386

End: 1008600

Strand: Direct

Name: hmp [H]

Synonym: BLi01006

Alternate gene names: 52784778

Gene position: 1007386-1008600 (Clockwise)

Preceding gene: 52784776

Following gene: 52784779

Centisome position: 23.86

GC content: 47.74

Gene sequence:

>1215_bases
ATGCTATCTGAAAAAACCATGCAAATTGTCAAATCGACCGCCCCTGTTTTAAAAGAAAAGGGAACAGAGATCACCACTTG
TTTTTACAAGCGGATGTTTGATGCCCACCCTGAATTGAAAAACATTTTCAACATGTCCCGACAGCAAACCGGCGGACAGC
CGAAAGCTTTGGCTTTTACTGTGCTGCAGGCGGCTGAAAACATCGACCGGCTCGAAGACCTGCTGCCGGTTGTCAAGCAG
ATCGGACATAAACACAAAAGTTTGCATGTGAAACCAGAGCACTATCCTATTGTCGGTCAGCACCTTTTGGAAGCAATTGA
AATCGTGCTCGGCGAAGCTGCGACAGAGGACATTCTCCAGGCATGGGCAGAAGCCTATGAAGAGATCGCCCGTGTATTTA
TTGAAGTCGAAAAACAAATGTATGAGGAAGACAAAAAGCAGGATGGAAATTGGGAAGGGTTTAAACCGTTTGTCATCATG
GATAAACGAGTCGAATCCGATACGATAACCTCCTTTTATTTGAAGCCCGCCGATGGAACGGCTTTGCCCGCTTTCAGCCC
CGGACAGTACGTATCTGTCTGCATTAAAATTCCGGGCGAACCATATTTCTTAACAAGGCAGTACAGCTTGTCAGATGCTT
GGAATAAAGACTACTACCGCATTTCAGTCAAATTGGAAGCGGAGGAAGGCCAACCGGTGGGAAAGGTATCAAGCTATCTG
CATGAGAACATGGAAATCGGCGGCAGCTTGGAAGTGAGCGCTCCCGCCGGAGATTTCACACTTTCCGAAGGAACGGATCG
TCCGGTTTATTTCATTAGCGCCGGATCAGGCATCACGCCTGTCATGAGCATGGTGCAAACGTTGGCTCAAAAAGAAAGCC
GCCGCGAGATCACATTCGTGCATGCCGCGAAAACTGAACGGCATCACGCTTTCAAGGAAGAAACGGAGAAGCTGCTCGGC
GCCAATCCGGCGAACCGTCTCCTGTTCGTCTACAGCAGAGGTGCTGAAGCGACGGAAGAGCATACTGTAAAAGGCCGGGT
GAATGAAGAGCTTTTGAAATCGGTCGTGATAGATCCAAATGGCGAATTTTATGTATGCGGTCCGCTTTCATTTATGAAAA
GCGTGATTGAAGGATTGCAAAACCTTGGCGTTTCGATGGAAAACATCCGCTATGAGAGCTTTGCTTCATCTCTTGATATG
CAAATAGCGAACTAA

Upstream 100 bases:

>100_bases
TTTAATCAAAAGTAACAGTCCTATTTAAAAAAGATCTTATTGCACCTTTAAAGATGTATTTCAAATACATTTTTAAAGTG
ACTGGAGAGGAGCAGGCGTT

Downstream 100 bases:

>100_bases
CCTCAAGAAGGCAGCTGTCAAACCGACAGCTGCCTCAGCGTGTCGACAAACCCTCGCATGCGTTGTCAGTCCTGCGCGTC
GGTGCTCACGAATTCCAACA

Product: Hmp

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MLSEKTMQIVKSTAPVLKEKGTEITTCFYKRMFDAHPELKNIFNMSRQQTGGQPKALAFTVLQAAENIDRLEDLLPVVKQ
IGHKHKSLHVKPEHYPIVGQHLLEAIEIVLGEAATEDILQAWAEAYEEIARVFIEVEKQMYEEDKKQDGNWEGFKPFVIM
DKRVESDTITSFYLKPADGTALPAFSPGQYVSVCIKIPGEPYFLTRQYSLSDAWNKDYYRISVKLEAEEGQPVGKVSSYL
HENMEIGGSLEVSAPAGDFTLSEGTDRPVYFISAGSGITPVMSMVQTLAQKESRREITFVHAAKTERHHAFKEETEKLLG
ANPANRLLFVYSRGAEATEEHTVKGRVNEELLKSVVIDPNGEFYVCGPLSFMKSVIEGLQNLGVSMENIRYESFASSLDM
QIAN

Sequences:

>Translated_404_residues
MLSEKTMQIVKSTAPVLKEKGTEITTCFYKRMFDAHPELKNIFNMSRQQTGGQPKALAFTVLQAAENIDRLEDLLPVVKQ
IGHKHKSLHVKPEHYPIVGQHLLEAIEIVLGEAATEDILQAWAEAYEEIARVFIEVEKQMYEEDKKQDGNWEGFKPFVIM
DKRVESDTITSFYLKPADGTALPAFSPGQYVSVCIKIPGEPYFLTRQYSLSDAWNKDYYRISVKLEAEEGQPVGKVSSYL
HENMEIGGSLEVSAPAGDFTLSEGTDRPVYFISAGSGITPVMSMVQTLAQKESRREITFVHAAKTERHHAFKEETEKLLG
ANPANRLLFVYSRGAEATEEHTVKGRVNEELLKSVVIDPNGEFYVCGPLSFMKSVIEGLQNLGVSMENIRYESFASSLDM
QIAN
>Mature_404_residues
MLSEKTMQIVKSTAPVLKEKGTEITTCFYKRMFDAHPELKNIFNMSRQQTGGQPKALAFTVLQAAENIDRLEDLLPVVKQ
IGHKHKSLHVKPEHYPIVGQHLLEAIEIVLGEAATEDILQAWAEAYEEIARVFIEVEKQMYEEDKKQDGNWEGFKPFVIM
DKRVESDTITSFYLKPADGTALPAFSPGQYVSVCIKIPGEPYFLTRQYSLSDAWNKDYYRISVKLEAEEGQPVGKVSSYL
HENMEIGGSLEVSAPAGDFTLSEGTDRPVYFISAGSGITPVMSMVQTLAQKESRREITFVHAAKTERHHAFKEETEKLLG
ANPANRLLFVYSRGAEATEEHTVKGRVNEELLKSVVIDPNGEFYVCGPLSFMKSVIEGLQNLGVSMENIRYESFASSLDM
QIAN

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=398, Percent_Identity=40.4522613065327, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI1787658, Length=218, Percent_Identity=29.8165137614679, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788104, Length=246, Percent_Identity=26.4227642276423, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321673, Length=411, Percent_Identity=41.6058394160584, Blast_Score=276, Evalue=6e-75,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR001834
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 45342; Mature: 45342

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSEKTMQIVKSTAPVLKEKGTEITTCFYKRMFDAHPELKNIFNMSRQQTGGQPKALAFT
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHH
VLQAAENIDRLEDLLPVVKQIGHKHKSLHVKPEHYPIVGQHLLEAIEIVLGEAATEDILQ
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
AWAEAYEEIARVFIEVEKQMYEEDKKQDGNWEGFKPFVIMDKRVESDTITSFYLKPADGT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCC
ALPAFSPGQYVSVCIKIPGEPYFLTRQYSLSDAWNKDYYRISVKLEAEEGQPVGKVSSYL
CCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCCCHHHHHHHH
HENMEIGGSLEVSAPAGDFTLSEGTDRPVYFISAGSGITPVMSMVQTLAQKESRREITFV
HHCCCCCCEEEECCCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEE
HAAKTERHHAFKEETEKLLGANPANRLLFVYSRGAEATEEHTVKGRVNEELLKSVVIDPN
EECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHCCCHHHHHHHHEEECCC
GEFYVCGPLSFMKSVIEGLQNLGVSMENIRYESFASSLDMQIAN
CCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECC
>Mature Secondary Structure
MLSEKTMQIVKSTAPVLKEKGTEITTCFYKRMFDAHPELKNIFNMSRQQTGGQPKALAFT
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHH
VLQAAENIDRLEDLLPVVKQIGHKHKSLHVKPEHYPIVGQHLLEAIEIVLGEAATEDILQ
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
AWAEAYEEIARVFIEVEKQMYEEDKKQDGNWEGFKPFVIMDKRVESDTITSFYLKPADGT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCC
ALPAFSPGQYVSVCIKIPGEPYFLTRQYSLSDAWNKDYYRISVKLEAEEGQPVGKVSSYL
CCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCCCHHHHHHHH
HENMEIGGSLEVSAPAGDFTLSEGTDRPVYFISAGSGITPVMSMVQTLAQKESRREITFV
HHCCCCCCEEEECCCCCCCEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEE
HAAKTERHHAFKEETEKLLGANPANRLLFVYSRGAEATEEHTVKGRVNEELLKSVVIDPN
EECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHCCCHHHHHHHHEEECCC
GEFYVCGPLSFMKSVIEGLQNLGVSMENIRYESFASSLDMQIAN
CCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]