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The map label for this gene is yhdA [H]

Identifier: 52784774

GI number: 52784774

Start: 1005347

End: 1005865

Strand: Direct

Name: yhdA [H]

Synonym: BLi01002

Alternate gene names: 52784774

Gene position: 1005347-1005865 (Clockwise)

Preceding gene: 52784773

Following gene: 52784776

Centisome position: 23.81

GC content: 48.17

Gene sequence:

>519_bases
ATGAAAATAACCGTAATTAGCGGAACTGCGAGAAAACAGGGCAGAACGCGGATCGCCGCTTCCTATATCGCCCGCGCTTA
TCAGGCTGAGCTTATCGATCTAAGCGAGTTCATCCTTCCGATTTTCAACGGAGACGAAGAGCAAAGCTCGCTGGAGAATG
TTCAAAAATTGAAAGCTTCGGTCAAAGAAGCAGACGCTGTCGTTTTATTATCTCCCGAATACCACAGCGGTATGAGCGGC
GCTTTGAAAAATGCACTCGATTTTTTAAGCAATGAGCAGTTCTCGGGCAAACCGGTGGCGCTTTTTGCTTGTGCAGGGGG
CGGCAAAGGCGGGATGAACGCGCTCGCCAATATGCGCATTGTCGCACGCGGAGTTTATGCGAACGTCATCACAAAGCAGC
TTGTGCTCGATCCGATTCATATTGATGAGGAGCACCAAACGGTTACTGAAGCAGCCAAAGCCAATATTAAAAATGTAATG
GAAGAGCTTCTGCTCTTTGCCGAATTTCAAAAAGCCTGA

Upstream 100 bases:

>100_bases
TCCAATCTGCTTTCAAAGCTTGGCTTATCCGATCGCACGCAAGCCGCGCTTTTTGCAGTAAAACACGGACTACAACAAAA
TGACGGGAGAGGATGACCAA

Downstream 100 bases:

>100_bases
CGCCGACAGAGCGCCAGGCTTATTTTATTTTACGTAAAGACAAGCACGCCGTTTTCGTGTTCATGTCCGGAAAATGAGGC
GTGATCAATATACTGCAGCA

Product: YhdA

Products: NA

Alternate protein names: Azobenzene reductase [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MKITVISGTARKQGRTRIAASYIARAYQAELIDLSEFILPIFNGDEEQSSLENVQKLKASVKEADAVVLLSPEYHSGMSG
ALKNALDFLSNEQFSGKPVALFACAGGGKGGMNALANMRIVARGVYANVITKQLVLDPIHIDEEHQTVTEAAKANIKNVM
EELLLFAEFQKA

Sequences:

>Translated_172_residues
MKITVISGTARKQGRTRIAASYIARAYQAELIDLSEFILPIFNGDEEQSSLENVQKLKASVKEADAVVLLSPEYHSGMSG
ALKNALDFLSNEQFSGKPVALFACAGGGKGGMNALANMRIVARGVYANVITKQLVLDPIHIDEEHQTVTEAAKANIKNVM
EELLLFAEFQKA
>Mature_172_residues
MKITVISGTARKQGRTRIAASYIARAYQAELIDLSEFILPIFNGDEEQSSLENVQKLKASVKEADAVVLLSPEYHSGMSG
ALKNALDFLSNEQFSGKPVALFACAGGGKGGMNALANMRIVARGVYANVITKQLVLDPIHIDEEHQTVTEAAKANIKNVM
EELLLFAEFQKA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADPH, but not NADH, as an electron donor for its activity [H]

COG id: COG0431

COG function: function code R; Predicted flavoprotein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005025 [H]

Pfam domain/function: PF03358 FMN_red [H]

EC number: NA

Molecular weight: Translated: 18641; Mature: 18641

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITVISGTARKQGRTRIAASYIARAYQAELIDLSEFILPIFNGDEEQSSLENVQKLKAS
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VKEADAVVLLSPEYHSGMSGALKNALDFLSNEQFSGKPVALFACAGGGKGGMNALANMRI
HHHCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
VARGVYANVITKQLVLDPIHIDEEHQTVTEAAKANIKNVMEELLLFAEFQKA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKITVISGTARKQGRTRIAASYIARAYQAELIDLSEFILPIFNGDEEQSSLENVQKLKAS
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VKEADAVVLLSPEYHSGMSGALKNALDFLSNEQFSGKPVALFACAGGGKGGMNALANMRI
HHHCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
VARGVYANVITKQLVLDPIHIDEEHQTVTEAAKANIKNVMEELLLFAEFQKA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]