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The map label for this gene is cspR [H]

Identifier: 52784729

GI number: 52784729

Start: 956518

End: 956991

Strand: Direct

Name: cspR [H]

Synonym: BLi00957

Alternate gene names: 52784729

Gene position: 956518-956991 (Clockwise)

Preceding gene: 52784728

Following gene: 52784730

Centisome position: 22.65

GC content: 44.73

Gene sequence:

>474_bases
TTGGCATTGCATGTCGTACTATATCAACCAGAAATTCCTGCAAACACAGGAAATATCGCACGGACTTGTGCAGCAACAGA
TACAACACTTCATTTAATCAGACCGCTCGGCTTTTCAACCGACGATAAAATGCTCAAGCGGGCAGGTCTTGATTATTGGG
AATTCGTCAATGTCGTCTACCACGACTCATTGGATGAATTGTTTGATGCCCATCCTGAAGGCCGTTTTTTCTTCATTACA
AAGTTCGGTCAAAAACCGCACACTTCATTTGATTATTCAGATCAAAACGAAGATTACTTCTTTGTCTTCGGCAGAGAGAC
GAACGGCTTGCCGAAAGAGCTGATCGAACAAAACATGGATCGCTGTCTGCGCCTGCCGATGACAGAGCACGTCAGATCAC
TGAACTTATCAAACACTGCGGCAATCCTCGTGTATGAGGCGCTTCGCCAGCAGAATTACAGAGATTTGACTTAA

Upstream 100 bases:

>100_bases
TCATCGATTCGATATGCGTTTTTTCATATATCAGATGATACGACAAAATCGAAATGAGTGGTATAATAAACAGCGGATTA
GAAAAACAGAGGTGAATACT

Downstream 100 bases:

>100_bases
TAGGTAAAGAGCCCTTTTTCAAAAGGGGCTCTTTTTTTGTTTTCCAATGTAACAGTGTTATGTTACAATGTGTGTGAAGG
AGCTGAAACAGATGGTTCAG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MALHVVLYQPEIPANTGNIARTCAATDTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHDSLDELFDAHPEGRFFFIT
KFGQKPHTSFDYSDQNEDYFFVFGRETNGLPKELIEQNMDRCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLT

Sequences:

>Translated_157_residues
MALHVVLYQPEIPANTGNIARTCAATDTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHDSLDELFDAHPEGRFFFIT
KFGQKPHTSFDYSDQNEDYFFVFGRETNGLPKELIEQNMDRCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLT
>Mature_156_residues
ALHVVLYQPEIPANTGNIARTCAATDTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVYHDSLDELFDAHPEGRFFFITK
FGQKPHTSFDYSDQNEDYFFVFGRETNGLPKELIEQNMDRCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLT

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=152, Percent_Identity=44.7368421052632, Blast_Score=136, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18225; Mature: 18094

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALHVVLYQPEIPANTGNIARTCAATDTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVY
CEEEEEEECCCCCCCCCCCEEEECCCCHHHEEHHCCCCCCHHHHHHHHCCCHHHHHHHHH
HDSLDELFDAHPEGRFFFITKFGQKPHTSFDYSDQNEDYFFVFGRETNGLPKELIEQNMD
HCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
RCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLT
HHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ALHVVLYQPEIPANTGNIARTCAATDTTLHLIRPLGFSTDDKMLKRAGLDYWEFVNVVY
EEEEEEECCCCCCCCCCCEEEECCCCHHHEEHHCCCCCCHHHHHHHHCCCHHHHHHHHH
HDSLDELFDAHPEGRFFFITKFGQKPHTSFDYSDQNEDYFFVFGRETNGLPKELIEQNMD
HCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
RCLRLPMTEHVRSLNLSNTAAILVYEALRQQNYRDLT
HHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]