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The map label for this gene is yfjO [H]

Identifier: 52784631

GI number: 52784631

Start: 851101

End: 852507

Strand: Direct

Name: yfjO [H]

Synonym: BLi00832

Alternate gene names: 52784631

Gene position: 851101-852507 (Clockwise)

Preceding gene: 52784630

Following gene: 52784632

Centisome position: 20.16

GC content: 52.52

Gene sequence:

>1407_bases
ATGGAGAGAACACCAGTGAAACGTGACAAAAAAGGATCATCAGCAGAATTAAAAGTCGGACAGCAATTTCCGCTGACGAT
CAAGCGCCTCGGCATCAATGGCGAAGGCGTCGGCTACTTCAAGAAAAAAGTCGTCTTCGTCCCCGGCGCGCTGCCGGGAG
AAGAAGTCGTCGTCGAGGCGACAAAGGTTCATCCGAAATTTTCCGAAGGGCGGGTCAAAAAGGTGCGCAAACGTTCAGAG
CACCGCGTCAAGCCGCCCTGCATCATCTATGAGCAATGCGGGGGCTGCCAGCTCCAGCATCTGGCTTATGAAGAGCAGCT
GAAAGGAAAACGGGACATCGTCATTCAATCGCTCGAACGGCACACCCGTTTTGACATCGGCAAAATGGACATCAGGCCGA
CGATCGGCATGGAAAACCCGTGGCACTATCGGAATAAAAGCCAGTTTCAGCTCGGGCTTTCGAAAAACGGAAAGATGGTG
GCCGGGCTGTACGGCCTTGACTCGCACCGTCTTGTCCCGATTACGGAATGCATTGTTCAGCATCCGGCGACGAACAAAAC
GACGCAAACCGTCAAGCAGGTTCTTGAAGACTTCGGCATTTCCGTCTACAACGAACGGAAGCGCACGGGCGATGTCCGCA
CGATCGTGACGAGGGTCGGCTTCGAGACGGGGGAAGTGCAGGTTGTCCTTGTCACGTCAAAAGAAGACCTGCCGCGCAAG
GAGCTCATTGCAGAAGAAATCAAGAAGCGTCTGCCTGAAGTGACATCTGTGATTCAAAACGTCAACAAAGCCAAAACATC
GGTTGTCTTCGGCGATGATACAAGGCTCTTAGCCGGCAATATGGTCATTCAGGAATTTCTCGGAGAGGTTTCATTTGAGC
TGAGCGCCCGCGCTTTTTTTCAGCTCAATCCGATCCAGACAGTCAAGCTGTATGACGAAGTGAAAAAAGCGGCAAAACTG
ACCGGGCGCGAAAAAGTCGTCGACGCATATTGCGGCGTCGGCACGATCGGAATGTGGATCGCCGACGGGGCAAAAGAAAT
CCGCGGCATGGACGTCATCAAAGAATCGATTGATGACGCCAACCAAAACGCCAAAAAGCACGGAATCGAAAATGCGCGCT
ATGTAACAGGAACGGCCGAGCACTGGCTGCCGAAATGGACAAAAGAAGGCTTCAGGCCCGACGTCGTCATCGTCGATCCG
CCAAGAACAGGCTGTGACCGCACGTTCCTTGATACGATCAGGCAGGTTAAGCCGAAGCGGTTCGTTTATGTCTCATGCAA
TCCGTCAACACTTGCGAAAGATTTGCAGTACTTGGCGAAGGATTACCATGTAGAATACATTCAGCCTGTCGATATGTTTC
CGCAGACGGCGCACGTCGAATCGGTCGTATCGCTGAAGCTGAAATAA

Upstream 100 bases:

>100_bases
ACGATAAACCGACAAAAGAACAAATGCTTGAAATGAGCAAAGAATGGCACCCTTATTTAAGCTATGCTTCCTTGTATTTA
TGGAGGAGCATCGAGTAGAA

Downstream 100 bases:

>100_bases
AACGCCTTTTTAAAGGCGTTTTTTAGCGGAAACAAAAGGGGGATACATGATGAAAAGACTTTGCATCATCCCGTGCGGGG
CGAAAAAAATCTGGGATGTT

Product: YfjO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MERTPVKRDKKGSSAELKVGQQFPLTIKRLGINGEGVGYFKKKVVFVPGALPGEEVVVEATKVHPKFSEGRVKKVRKRSE
HRVKPPCIIYEQCGGCQLQHLAYEEQLKGKRDIVIQSLERHTRFDIGKMDIRPTIGMENPWHYRNKSQFQLGLSKNGKMV
AGLYGLDSHRLVPITECIVQHPATNKTTQTVKQVLEDFGISVYNERKRTGDVRTIVTRVGFETGEVQVVLVTSKEDLPRK
ELIAEEIKKRLPEVTSVIQNVNKAKTSVVFGDDTRLLAGNMVIQEFLGEVSFELSARAFFQLNPIQTVKLYDEVKKAAKL
TGREKVVDAYCGVGTIGMWIADGAKEIRGMDVIKESIDDANQNAKKHGIENARYVTGTAEHWLPKWTKEGFRPDVVIVDP
PRTGCDRTFLDTIRQVKPKRFVYVSCNPSTLAKDLQYLAKDYHVEYIQPVDMFPQTAHVESVVSLKLK

Sequences:

>Translated_468_residues
MERTPVKRDKKGSSAELKVGQQFPLTIKRLGINGEGVGYFKKKVVFVPGALPGEEVVVEATKVHPKFSEGRVKKVRKRSE
HRVKPPCIIYEQCGGCQLQHLAYEEQLKGKRDIVIQSLERHTRFDIGKMDIRPTIGMENPWHYRNKSQFQLGLSKNGKMV
AGLYGLDSHRLVPITECIVQHPATNKTTQTVKQVLEDFGISVYNERKRTGDVRTIVTRVGFETGEVQVVLVTSKEDLPRK
ELIAEEIKKRLPEVTSVIQNVNKAKTSVVFGDDTRLLAGNMVIQEFLGEVSFELSARAFFQLNPIQTVKLYDEVKKAAKL
TGREKVVDAYCGVGTIGMWIADGAKEIRGMDVIKESIDDANQNAKKHGIENARYVTGTAEHWLPKWTKEGFRPDVVIVDP
PRTGCDRTFLDTIRQVKPKRFVYVSCNPSTLAKDLQYLAKDYHVEYIQPVDMFPQTAHVESVVSLKLK
>Mature_468_residues
MERTPVKRDKKGSSAELKVGQQFPLTIKRLGINGEGVGYFKKKVVFVPGALPGEEVVVEATKVHPKFSEGRVKKVRKRSE
HRVKPPCIIYEQCGGCQLQHLAYEEQLKGKRDIVIQSLERHTRFDIGKMDIRPTIGMENPWHYRNKSQFQLGLSKNGKMV
AGLYGLDSHRLVPITECIVQHPATNKTTQTVKQVLEDFGISVYNERKRTGDVRTIVTRVGFETGEVQVVLVTSKEDLPRK
ELIAEEIKKRLPEVTSVIQNVNKAKTSVVFGDDTRLLAGNMVIQEFLGEVSFELSARAFFQLNPIQTVKLYDEVKKAAKL
TGREKVVDAYCGVGTIGMWIADGAKEIRGMDVIKESIDDANQNAKKHGIENARYVTGTAEHWLPKWTKEGFRPDVVIVDP
PRTGCDRTFLDTIRQVKPKRFVYVSCNPSTLAKDLQYLAKDYHVEYIQPVDMFPQTAHVESVVSLKLK

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=349, Percent_Identity=26.0744985673352, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI51173878, Length=349, Percent_Identity=26.0744985673352, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI269784680, Length=423, Percent_Identity=26.4775413711584, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI269784676, Length=423, Percent_Identity=26.4775413711584, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI20149688, Length=423, Percent_Identity=26.4775413711584, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI269784678, Length=399, Percent_Identity=26.3157894736842, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1789148, Length=450, Percent_Identity=25.1111111111111, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787083, Length=387, Percent_Identity=24.031007751938, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1790403, Length=183, Percent_Identity=26.775956284153, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71997438, Length=163, Percent_Identity=30.0613496932515, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322909, Length=436, Percent_Identity=26.605504587156, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24666579, Length=414, Percent_Identity=26.5700483091787, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 52861; Mature: 52861

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERTPVKRDKKGSSAELKVGQQFPLTIKRLGINGEGVGYFKKKVVFVPGALPGEEVVVEA
CCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCEEEEE
TKVHPKFSEGRVKKVRKRSEHRVKPPCIIYEQCGGCQLQHLAYEEQLKGKRDIVIQSLER
ECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHCCCHHHHHHHHHH
HTRFDIGKMDIRPTIGMENPWHYRNKSQFQLGLSKNGKMVAGLYGLDSHRLVPITECIVQ
HHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEHHHHHHHC
HPATNKTTQTVKQVLEDFGISVYNERKRTGDVRTIVTRVGFETGEVQVVLVTSKEDLPRK
CCCCCHHHHHHHHHHHHCCCEEECCHHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHH
ELIAEEIKKRLPEVTSVIQNVNKAKTSVVFGDDTRLLAGNMVIQEFLGEVSFELSARAFF
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEHHHHHHHHHHHHHCCEECEEEEE
QLNPIQTVKLYDEVKKAAKLTGREKVVDAYCGVGTIGMWIADGAKEIRGMDVIKESIDDA
EECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
NQNAKKHGIENARYVTGTAEHWLPKWTKEGFRPDVVIVDPPRTGCDRTFLDTIRQVKPKR
HCCHHHCCCCCCEEEECCHHHHCCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCE
FVYVSCNPSTLAKDLQYLAKDYHVEYIQPVDMFPQTAHVESVVSLKLK
EEEEECCHHHHHHHHHHHHHHCCEEEECCHHCCCCHHHHHHHHEEECC
>Mature Secondary Structure
MERTPVKRDKKGSSAELKVGQQFPLTIKRLGINGEGVGYFKKKVVFVPGALPGEEVVVEA
CCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCEEEEE
TKVHPKFSEGRVKKVRKRSEHRVKPPCIIYEQCGGCQLQHLAYEEQLKGKRDIVIQSLER
ECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHCCCHHHHHHHHHH
HTRFDIGKMDIRPTIGMENPWHYRNKSQFQLGLSKNGKMVAGLYGLDSHRLVPITECIVQ
HHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEHHHHHHHC
HPATNKTTQTVKQVLEDFGISVYNERKRTGDVRTIVTRVGFETGEVQVVLVTSKEDLPRK
CCCCCHHHHHHHHHHHHCCCEEECCHHHCCHHHHHHHHHCCCCCCEEEEEEECCCCCCHH
ELIAEEIKKRLPEVTSVIQNVNKAKTSVVFGDDTRLLAGNMVIQEFLGEVSFELSARAFF
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEHHHHHHHHHHHHHCCEECEEEEE
QLNPIQTVKLYDEVKKAAKLTGREKVVDAYCGVGTIGMWIADGAKEIRGMDVIKESIDDA
EECCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHH
NQNAKKHGIENARYVTGTAEHWLPKWTKEGFRPDVVIVDPPRTGCDRTFLDTIRQVKPKR
HCCHHHCCCCCCEEEECCHHHHCCHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCE
FVYVSCNPSTLAKDLQYLAKDYHVEYIQPVDMFPQTAHVESVVSLKLK
EEEEECCHHHHHHHHHHHHHHCCEEEECCHHCCCCHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]