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The map label for this gene is gldA [H]

Identifier: 52784627

GI number: 52784627

Start: 847200

End: 848303

Strand: Direct

Name: gldA [H]

Synonym: BLi00828

Alternate gene names: 52784627

Gene position: 847200-848303 (Clockwise)

Preceding gene: 52784626

Following gene: 52784630

Centisome position: 20.06

GC content: 50.54

Gene sequence:

>1104_bases
ATGTCAAAATCAGTAAAATCAGTCACATCACCTAAAAAATTTATTACAGGAAAACGACTGCTGGAGAACTTGAACGACTA
CATTGAAGATTTTGGCGACAACGCATATATCATTTGCGATGAATTCATTTTGGAACGCGCTCAAAAAGAAGCGGGGAATT
CGATTCAGAAAGCCGGCAATCAAGCCGTTTTTGAAAAATTCAATTACGAATGCACACAGGAAGAAATCGATCGCAACCGG
GAGCTTGCACGCAATGCAGGCGCTAATATCATCGTTGGGATCGGAGGCGGTAAAACGCTTGATACCGCAAAAGCCACCGC
TTATTACGAGAAGCTGCCGGTTGTGATTTTCCCGACAATTGCTTCTACGGATGCTCCATGTACGGCCCTTGCCGTCATTT
ATAAACACGACGGATCGTTTGACCGCTATCTGTTTTTGCCGACGAACCCAGATGTCGTTCTTGCGGACTCTGAGATTTTG
GCATCCGCGCCGCCGCGCTTTTTCGCAGCCGGTATCGGTGACGCCTTGGCGACGTATTTTGAAGCGCGTGCCTGCTTTAA
AGCAAACGGCGATAACCTCGTGCTGATGAAGCCTTCAACAACTGGATTGGGACTTGCCCGTCTTTGCTATGATACGCTGT
TGGAAAACGGTGTGAAAGCGATGCAGGCGGTTAAGCACGGCGTTTCCACACGAGCGGTCGAAGATACAATCGAGGCGACC
ATCTATTTAAGCGGCGTCGGTGCCGAATCAGGCGGTCTTGCCGCCGCACACGCGATCCACAACGGAATGACAGCCGTTCC
TTCTCTGCACAGGGCTCAGCACGGCGAAAAAGTCACGTTCGGCCTTTTGGCGCAGCTTGTTCTTGAAAACGCGCCGGCCG
AAGAATTGGAGACCGTTATTGACTTTATCAAAGGCGTCGGTCTTCCGTTGACATTAAAAGACCTCGGAGTCGACGAATTT
GTCGAAGAAGAATGGCGCCAAGTCGCTCAAAGCGCTTGCGCGGAAGGCGACACAATGGGCAACATGCCGTTCCCAGTCAC
CCCTGACGACGTCTACAATGCGATCGTCGCCGCCAACGCGATTGCAGAATCTTATCACGATTAA

Upstream 100 bases:

>100_bases
CCCTAGTCAATTCTATAGAATAAAAATCCATTTTATATACATATATTACTAGATTTAAAAAGAAAATAGGTATATCATTG
ATAGTGAAGGGGGAATTACC

Downstream 100 bases:

>100_bases
TAGACGAAAAGCCGTTTCCGTGAAGGAGCGGCTTTCTGCTTTGGGTTAGTATATTAGTTTGTGAATCGGCGGTCTGTCCG
TGCTTTTGAATATGAATTGT

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSKSVKSVTSPKKFITGKRLLENLNDYIEDFGDNAYIICDEFILERAQKEAGNSIQKAGNQAVFEKFNYECTQEEIDRNR
ELARNAGANIIVGIGGGKTLDTAKATAYYEKLPVVIFPTIASTDAPCTALAVIYKHDGSFDRYLFLPTNPDVVLADSEIL
ASAPPRFFAAGIGDALATYFEARACFKANGDNLVLMKPSTTGLGLARLCYDTLLENGVKAMQAVKHGVSTRAVEDTIEAT
IYLSGVGAESGGLAAAHAIHNGMTAVPSLHRAQHGEKVTFGLLAQLVLENAPAEELETVIDFIKGVGLPLTLKDLGVDEF
VEEEWRQVAQSACAEGDTMGNMPFPVTPDDVYNAIVAANAIAESYHD

Sequences:

>Translated_367_residues
MSKSVKSVTSPKKFITGKRLLENLNDYIEDFGDNAYIICDEFILERAQKEAGNSIQKAGNQAVFEKFNYECTQEEIDRNR
ELARNAGANIIVGIGGGKTLDTAKATAYYEKLPVVIFPTIASTDAPCTALAVIYKHDGSFDRYLFLPTNPDVVLADSEIL
ASAPPRFFAAGIGDALATYFEARACFKANGDNLVLMKPSTTGLGLARLCYDTLLENGVKAMQAVKHGVSTRAVEDTIEAT
IYLSGVGAESGGLAAAHAIHNGMTAVPSLHRAQHGEKVTFGLLAQLVLENAPAEELETVIDFIKGVGLPLTLKDLGVDEF
VEEEWRQVAQSACAEGDTMGNMPFPVTPDDVYNAIVAANAIAESYHD
>Mature_366_residues
SKSVKSVTSPKKFITGKRLLENLNDYIEDFGDNAYIICDEFILERAQKEAGNSIQKAGNQAVFEKFNYECTQEEIDRNRE
LARNAGANIIVGIGGGKTLDTAKATAYYEKLPVVIFPTIASTDAPCTALAVIYKHDGSFDRYLFLPTNPDVVLADSEILA
SAPPRFFAAGIGDALATYFEARACFKANGDNLVLMKPSTTGLGLARLCYDTLLENGVKAMQAVKHGVSTRAVEDTIEATI
YLSGVGAESGGLAAAHAIHNGMTAVPSLHRAQHGEKVTFGLLAQLVLENAPAEELETVIDFIKGVGLPLTLKDLGVDEFV
EEEWRQVAQSACAEGDTMGNMPFPVTPDDVYNAIVAANAIAESYHD

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions [H]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=360, Percent_Identity=51.9444444444444, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1786815, Length=359, Percent_Identity=29.2479108635098, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 39477; Mature: 39346

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSVKSVTSPKKFITGKRLLENLNDYIEDFGDNAYIICDEFILERAQKEAGNSIQKAGN
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCHHHHCCC
QAVFEKFNYECTQEEIDRNRELARNAGANIIVGIGGGKTLDTAKATAYYEKLPVVIFPTI
HHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCEEEEECC
ASTDAPCTALAVIYKHDGSFDRYLFLPTNPDVVLADSEILASAPPRFFAAGIGDALATYF
CCCCCCHHHEEEEEECCCCCCEEEEEECCCCEEEECCHHHHCCCCHHHHHHHHHHHHHHH
EARACFKANGDNLVLMKPSTTGLGLARLCYDTLLENGVKAMQAVKHGVSTRAVEDTIEAT
HHHHHEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEE
IYLSGVGAESGGLAAAHAIHNGMTAVPSLHRAQHGEKVTFGLLAQLVLENAPAEELETVI
EEEEECCCCCCCHHHHHHHHCCCHHCCHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHH
DFIKGVGLPLTLKDLGVDEFVEEEWRQVAQSACAEGDTMGNMPFPVTPDDVYNAIVAANA
HHHHCCCCCEEHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
IAESYHD
HHHHCCC
>Mature Secondary Structure 
SKSVKSVTSPKKFITGKRLLENLNDYIEDFGDNAYIICDEFILERAQKEAGNSIQKAGN
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCHHHHCCC
QAVFEKFNYECTQEEIDRNRELARNAGANIIVGIGGGKTLDTAKATAYYEKLPVVIFPTI
HHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCEEEEECC
ASTDAPCTALAVIYKHDGSFDRYLFLPTNPDVVLADSEILASAPPRFFAAGIGDALATYF
CCCCCCHHHEEEEEECCCCCCEEEEEECCCCEEEECCHHHHCCCCHHHHHHHHHHHHHHH
EARACFKANGDNLVLMKPSTTGLGLARLCYDTLLENGVKAMQAVKHGVSTRAVEDTIEAT
HHHHHEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEE
IYLSGVGAESGGLAAAHAIHNGMTAVPSLHRAQHGEKVTFGLLAQLVLENAPAEELETVI
EEEEECCCCCCCHHHHHHHHCCCHHCCHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHH
DFIKGVGLPLTLKDLGVDEFVEEEWRQVAQSACAEGDTMGNMPFPVTPDDVYNAIVAANA
HHHHCCCCCEEHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
IAESYHD
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]