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The map label for this gene is yflA [H]

Identifier: 52784594

GI number: 52784594

Start: 804832

End: 806244

Strand: Direct

Name: yflA [H]

Synonym: BLi00795

Alternate gene names: 52784594

Gene position: 804832-806244 (Clockwise)

Preceding gene: 52784593

Following gene: 52784595

Centisome position: 19.06

GC content: 51.52

Gene sequence:

>1413_bases
ATGGAACGATTATTGAATTGGGTTGAATACATATCAGATTGGCTGTGGGGGCCACCGCTGATCATACTGCTGACAGGGAC
AGGAGTCTACTTGACGATCCTGCTGAAATTTTTTCAATTTCGCTATCCTCTCTATATCTTTAAGCAGACGATCGGAAGTG
TCGGCAAAAAGCCGAAAGGAGAAGGGACGGTTACACCGCTGCAGGCTTTGACATCGGCTCTCAGCTCAACAGTCGGCGCC
GCCAATATCGTCGGTGTGCCTGCGGCCATCATGTTCGGCGGTCCGGGAGCCGTTTTTTGGATGTGGCTGGTCGCGCTGTT
TGCCATGGCGATCAAGTTTTCGGAAAGCGTCCTCGCCGTCCACTACAGAGAAAAAAATGAAGAAGGCGAATATGTCGGTG
GACCGATGTATTACATTACAAAAGGGCTCTCCATGAAATGGCTCGGCGTCTTTTTTTCAGTAGCGCTGATGATCGAACTT
GTACCGAGCATCATGGTGCAGGGAAATTCGGTTTCCGCATCGCTGGCTGAGACGTTCTCGTTCAATCGTTTATACACCGG
AATCGGGATTGCTTTTTTGATCGGTTTAGTGGTGATCGGAGGGATTAAACGGATTGGAAAAGTGACGGAGCTCGCAGTTC
CGCTTATGGCAGGGGCGTACATCGGGGCCGGTTTCCTGATTGTCGTCACAAACCTTTCCTCTGTACCCGCTTTTTTCTCT
CTCATCTTTTCGAATGCGTTTACATCTTCTTCGGCATTCGGCGGCTTTGCAGGTGCGGCAGTTGCCGAAACCGTTCGCTG
GGGGTTTGCACGAGGCCTTTATTCCAACGAAGCCGGGATGGGGACGGCGCCGATTGCCCACGCAGCGGCGATGACCGACC
ACCCTGTGAGACAGGGGTTCTGGGCGGTGATCGGCATCGTGGTCGATACGCTGCTGATTTGCACGACCACCGCCTTCGTC
GTGCTCGCATCAGGCGTCTGGACCGGCGAAAACGCATCAAGCGATCCGGGGGCGCTGGCCGCAGCCGCATTTCGCAGCTA
TTTTGGCCCTGTGGGCGGATACTTCGTCTCTGTCTCGCTCGTTTTCTTTGTCTTATCAACGATCACAGTGGTCATTTTTT
ACGGTGTAAAACAAGCTGAATTTCTGTTCGGACGGCTCGCGGGACATGTGATTAAATTCATCTACCTGGCCGCCGTCGTC
ATCGGTGCTGCCGGAGGAGCAAAGGCGATATGGGGATTCCTCGATCTCGCGCTCGTTTTCATTCTTCTTCCGAATATTTT
GGCGGTGCTGCTCTTGAGCAGAAAAGTAAGGTCTTTATATGATGAGTTTTTTACATCAGAAAAGTATTATTTGAAAGACA
TCGGTCAGCAGGACGGCGTTTATGAAGGTGACGAAAAAGTGAACAATTTATGA

Upstream 100 bases:

>100_bases
AAAGTGTACCGAAGGCTGAGCGGAAATAAAAAGCGCTAAAAGCAGGTTTATTTCTCCCCTGTAGTGGTATTGACCCGTAA
ACGAACAAAAGGGTGATAGG

Downstream 100 bases:

>100_bases
TCACAGCCGGTTTGTTCCCGGCTCATAAATCGGAGGAAGCCTTGCTCTCCTACGTTTTATGAAGGGAACATCCGGCTTTT
TTAATTTTTGAAAGCGCTAC

Product: YflA

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MERLLNWVEYISDWLWGPPLIILLTGTGVYLTILLKFFQFRYPLYIFKQTIGSVGKKPKGEGTVTPLQALTSALSSTVGA
ANIVGVPAAIMFGGPGAVFWMWLVALFAMAIKFSESVLAVHYREKNEEGEYVGGPMYYITKGLSMKWLGVFFSVALMIEL
VPSIMVQGNSVSASLAETFSFNRLYTGIGIAFLIGLVVIGGIKRIGKVTELAVPLMAGAYIGAGFLIVVTNLSSVPAFFS
LIFSNAFTSSSAFGGFAGAAVAETVRWGFARGLYSNEAGMGTAPIAHAAAMTDHPVRQGFWAVIGIVVDTLLICTTTAFV
VLASGVWTGENASSDPGALAAAAFRSYFGPVGGYFVSVSLVFFVLSTITVVIFYGVKQAEFLFGRLAGHVIKFIYLAAVV
IGAAGGAKAIWGFLDLALVFILLPNILAVLLLSRKVRSLYDEFFTSEKYYLKDIGQQDGVYEGDEKVNNL

Sequences:

>Translated_470_residues
MERLLNWVEYISDWLWGPPLIILLTGTGVYLTILLKFFQFRYPLYIFKQTIGSVGKKPKGEGTVTPLQALTSALSSTVGA
ANIVGVPAAIMFGGPGAVFWMWLVALFAMAIKFSESVLAVHYREKNEEGEYVGGPMYYITKGLSMKWLGVFFSVALMIEL
VPSIMVQGNSVSASLAETFSFNRLYTGIGIAFLIGLVVIGGIKRIGKVTELAVPLMAGAYIGAGFLIVVTNLSSVPAFFS
LIFSNAFTSSSAFGGFAGAAVAETVRWGFARGLYSNEAGMGTAPIAHAAAMTDHPVRQGFWAVIGIVVDTLLICTTTAFV
VLASGVWTGENASSDPGALAAAAFRSYFGPVGGYFVSVSLVFFVLSTITVVIFYGVKQAEFLFGRLAGHVIKFIYLAAVV
IGAAGGAKAIWGFLDLALVFILLPNILAVLLLSRKVRSLYDEFFTSEKYYLKDIGQQDGVYEGDEKVNNL
>Mature_470_residues
MERLLNWVEYISDWLWGPPLIILLTGTGVYLTILLKFFQFRYPLYIFKQTIGSVGKKPKGEGTVTPLQALTSALSSTVGA
ANIVGVPAAIMFGGPGAVFWMWLVALFAMAIKFSESVLAVHYREKNEEGEYVGGPMYYITKGLSMKWLGVFFSVALMIEL
VPSIMVQGNSVSASLAETFSFNRLYTGIGIAFLIGLVVIGGIKRIGKVTELAVPLMAGAYIGAGFLIVVTNLSSVPAFFS
LIFSNAFTSSSAFGGFAGAAVAETVRWGFARGLYSNEAGMGTAPIAHAAAMTDHPVRQGFWAVIGIVVDTLLICTTTAFV
VLASGVWTGENASSDPGALAAAAFRSYFGPVGGYFVSVSLVFFVLSTITVVIFYGVKQAEFLFGRLAGHVIKFIYLAAVV
IGAAGGAKAIWGFLDLALVFILLPNILAVLLLSRKVRSLYDEFFTSEKYYLKDIGQQDGVYEGDEKVNNL

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=469, Percent_Identity=34.1151385927505, Blast_Score=227, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 50446; Mature: 50446

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLLNWVEYISDWLWGPPLIILLTGTGVYLTILLKFFQFRYPLYIFKQTIGSVGKKPKG
CHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCC
EGTVTPLQALTSALSSTVGAANIVGVPAAIMFGGPGAVFWMWLVALFAMAIKFSESVLAV
CCCCCHHHHHHHHHHHHCCCHHHHCCCHHEEECCCHHHHHHHHHHHHHHHHHHCCHHHEE
HYREKNEEGEYVGGPMYYITKGLSMKWLGVFFSVALMIELVPSIMVQGNSVSASLAETFS
EECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FNRLYTGIGIAFLIGLVVIGGIKRIGKVTELAVPLMAGAYIGAGFLIVVTNLSSVPAFFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCHHHHHHHH
LIFSNAFTSSSAFGGFAGAAVAETVRWGFARGLYSNEAGMGTAPIAHAAAMTDHPVRQGF
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
WAVIGIVVDTLLICTTTAFVVLASGVWTGENASSDPGALAAAAFRSYFGPVGGYFVSVSL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
VFFVLSTITVVIFYGVKQAEFLFGRLAGHVIKFIYLAAVVIGAAGGAKAIWGFLDLALVF
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ILLPNILAVLLLSRKVRSLYDEFFTSEKYYLKDIGQQDGVYEGDEKVNNL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MERLLNWVEYISDWLWGPPLIILLTGTGVYLTILLKFFQFRYPLYIFKQTIGSVGKKPKG
CHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCC
EGTVTPLQALTSALSSTVGAANIVGVPAAIMFGGPGAVFWMWLVALFAMAIKFSESVLAV
CCCCCHHHHHHHHHHHHCCCHHHHCCCHHEEECCCHHHHHHHHHHHHHHHHHHCCHHHEE
HYREKNEEGEYVGGPMYYITKGLSMKWLGVFFSVALMIELVPSIMVQGNSVSASLAETFS
EECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FNRLYTGIGIAFLIGLVVIGGIKRIGKVTELAVPLMAGAYIGAGFLIVVTNLSSVPAFFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCHHHHHHHH
LIFSNAFTSSSAFGGFAGAAVAETVRWGFARGLYSNEAGMGTAPIAHAAAMTDHPVRQGF
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHH
WAVIGIVVDTLLICTTTAFVVLASGVWTGENASSDPGALAAAAFRSYFGPVGGYFVSVSL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
VFFVLSTITVVIFYGVKQAEFLFGRLAGHVIKFIYLAAVVIGAAGGAKAIWGFLDLALVF
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ILLPNILAVLLLSRKVRSLYDEFFTSEKYYLKDIGQQDGVYEGDEKVNNL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]