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The map label for this gene is yflG [H]

Identifier: 52784591

GI number: 52784591

Start: 801284

End: 802036

Strand: Reverse

Name: yflG [H]

Synonym: BLi00792

Alternate gene names: 52784591

Gene position: 802036-801284 (Counterclockwise)

Preceding gene: 52784592

Following gene: 52784590

Centisome position: 18.99

GC content: 48.87

Gene sequence:

>753_bases
GTGATTGTAACGAATGACCAAGAGCTGGAAGGGCTCAAGAAAATCGGCAGAATCGTCGCTTTAGCCAGGGAGGAAATGAA
GGCAAAAGCAGCTCCCGGCATGAGCACAAAAGAGCTGGATCTCATCGGAAAAGAAGTGCTTGAAGCCCATGGCGCCGCAT
CGGCTCCGGAAAAAGAATACGACTTCCCGGGAACGACGTGCATCAGTGTGAACGATGAAGTCGCCCACGGCATCCCAAGC
GAATCCAGAATTTTAAAAGAAGGGGATTTGATCAACATCGACATCTCCGCTGAATTTGGCGGCTACTATTCTGATACGGG
CATTTCTTTTGTACTCGGAAAAGGCGATGAAATGCTGCACAAGCTTTGCCAAAGTGCGGAAAGCGCTTTTCAAAAGGGGC
TTTTGCAGGCGCGCGCCGGCAAGAAACAAAACCAAATCGGCAGAGCCGTATACAATGAAGCAAAAGCAAACGGATTTACC
GTCATTAAAAACCTGACGGGACACGGGATTGGAAGGAACCTCCATGAAGCGCCCAACCATATCTTAAATTACTATGACCC
GTTTGATAATGCGCTCTTTAAAAACGGAACCGTCATCGCCCTTGAGCCGTTTGTTTCCACAAAAGCAGAACATACCATCG
AAGCCGGAGACGGCTGGACATTCAAAACGCCTGACGGCAGCTTTGTGGCGCAGATCGAGCATACGATCGTCATCACGAAA
AACGAACCGATCATTTTAACACAGCTTGACTGA

Upstream 100 bases:

>100_bases
AGCGGGTGCTTTTTTTGTCTTGAAGCCGCTCTGTTCCTTTTTCGCAAACAAATATGGTAAGCTTGAAATGGAATATAAAA
AAAGAAAGCGGGAATGAATT

Downstream 100 bases:

>100_bases
TTACGCATAAAAAAGCCCGGCTCATAGAAGCGCGGGCTTTTATGCTAAAATAAAAGACAAACTGTGCCGAAAGGAGCTTT
ACCGCATGCTGTTTGCCATC

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP 2 [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MIVTNDQELEGLKKIGRIVALAREEMKAKAAPGMSTKELDLIGKEVLEAHGAASAPEKEYDFPGTTCISVNDEVAHGIPS
ESRILKEGDLINIDISAEFGGYYSDTGISFVLGKGDEMLHKLCQSAESAFQKGLLQARAGKKQNQIGRAVYNEAKANGFT
VIKNLTGHGIGRNLHEAPNHILNYYDPFDNALFKNGTVIALEPFVSTKAEHTIEAGDGWTFKTPDGSFVAQIEHTIVITK
NEPIILTQLD

Sequences:

>Translated_250_residues
MIVTNDQELEGLKKIGRIVALAREEMKAKAAPGMSTKELDLIGKEVLEAHGAASAPEKEYDFPGTTCISVNDEVAHGIPS
ESRILKEGDLINIDISAEFGGYYSDTGISFVLGKGDEMLHKLCQSAESAFQKGLLQARAGKKQNQIGRAVYNEAKANGFT
VIKNLTGHGIGRNLHEAPNHILNYYDPFDNALFKNGTVIALEPFVSTKAEHTIEAGDGWTFKTPDGSFVAQIEHTIVITK
NEPIILTQLD
>Mature_250_residues
MIVTNDQELEGLKKIGRIVALAREEMKAKAAPGMSTKELDLIGKEVLEAHGAASAPEKEYDFPGTTCISVNDEVAHGIPS
ESRILKEGDLINIDISAEFGGYYSDTGISFVLGKGDEMLHKLCQSAESAFQKGLLQARAGKKQNQIGRAVYNEAKANGFT
VIKNLTGHGIGRNLHEAPNHILNYYDPFDNALFKNGTVIALEPFVSTKAEHTIEAGDGWTFKTPDGSFVAQIEHTIVITK
NEPIILTQLD

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=247, Percent_Identity=32.7935222672065, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI40385867, Length=252, Percent_Identity=32.5396825396825, Blast_Score=131, Evalue=7e-31,
Organism=Escherichia coli, GI1786364, Length=246, Percent_Identity=35.7723577235772, Blast_Score=168, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI71996291, Length=249, Percent_Identity=33.7349397590361, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323273, Length=241, Percent_Identity=31.5352697095436, Blast_Score=132, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21355531, Length=246, Percent_Identity=32.1138211382114, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24583427, Length=246, Percent_Identity=30.0813008130081, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 27154; Mature: 27154

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTNDQELEGLKKIGRIVALAREEMKAKAAPGMSTKELDLIGKEVLEAHGAASAPEKEY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
DFPGTTCISVNDEVAHGIPSESRILKEGDLINIDISAEFGGYYSDTGISFVLGKGDEMLH
CCCCCEEEEECHHHHHCCCCHHHEECCCCEEEEEEEECCCCEECCCCCEEEECCCHHHHH
KLCQSAESAFQKGLLQARAGKKQNQIGRAVYNEAKANGFTVIKNLTGHGIGRNLHEAPNH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHH
ILNYYDPFDNALFKNGTVIALEPFVSTKAEHTIEAGDGWTFKTPDGSFVAQIEHTIVITK
HHCCCCCCCHHHHCCCCEEEECCCCCCCCCCEEECCCCEEEECCCCCEEEEEEEEEEEEC
NEPIILTQLD
CCCEEEEECC
>Mature Secondary Structure
MIVTNDQELEGLKKIGRIVALAREEMKAKAAPGMSTKELDLIGKEVLEAHGAASAPEKEY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
DFPGTTCISVNDEVAHGIPSESRILKEGDLINIDISAEFGGYYSDTGISFVLGKGDEMLH
CCCCCEEEEECHHHHHCCCCHHHEECCCCEEEEEEEECCCCEECCCCCEEEECCCHHHHH
KLCQSAESAFQKGLLQARAGKKQNQIGRAVYNEAKANGFTVIKNLTGHGIGRNLHEAPNH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHH
ILNYYDPFDNALFKNGTVIALEPFVSTKAEHTIEAGDGWTFKTPDGSFVAQIEHTIVITK
HHCCCCCCCHHHHCCCCEEEECCCCCCCCCCEEECCCCEEEECCCCCEEEEEEEEEEEEC
NEPIILTQLD
CCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]