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The map label for this gene is yflS [H]

Identifier: 52784579

GI number: 52784579

Start: 791456

End: 792892

Strand: Direct

Name: yflS [H]

Synonym: BLi00780

Alternate gene names: 52784579

Gene position: 791456-792892 (Clockwise)

Preceding gene: 52784578

Following gene: 52784580

Centisome position: 18.74

GC content: 49.55

Gene sequence:

>1437_bases
GTGGCTAAAGAAAAAGATGCAGAACAAAAATCGGCACTGAACATCATTCCTCTTGCGGTCACGATTTTAGTCGGAGCGGT
TATTTGGTTTATTCCGTCGCCCGCCGGATTGGACCCGAAAGCATGGCACTTATTTGCAATCTTTGTGGCAACGATTATCG
GCTTTATATCAAAACCGCTGCCGATGGGGGCAATCGCCGTATTCGCATTGGCGATCACGGCGCTCACTGGAACGCTGTCG
ATTGAAGAAACGCTCAGCGGGTTTGGAAACAAGACGATTTGGCTGATCGTCATTGCCTTTTTTATTTCGCGGGGATTTAT
TAAAACCGGTTTAGGTGCGCGAATCGCCTATTTATTTGTACGGATGTTCGGCAAAAAAACACTCGGACTCTCGTATTCAC
TCCTTGTCAGCGATTTGATTTTATCGCCGGCGATCCCGAGTAATACCGCACGTGCCGGGGGAATTATTTTTCCGATTATC
CGTTCGTTATCCGAAACTTTTCAGTCGACGCCCAAGGACGGCACGGAACGGCGCATCGGCGCTTTCTTGCTGAAGGTGGG
CTTTCAGGGGAACTTGATCACTTCTGCGATGTTCTTAACGGCTATGGCGGCAAACCCGCTGATTGCCAAACTTGCCCATG
ATGTGGCAGGTGTGAATCTGACCTGGACAAGCTGGGCTGTGGCGGCCATCGTCCCGGGACTTGTCAGCTTGATCATCACC
CCGCTTGTCATTTATAAACTGTATCCGCCTGAGGTGAAAAAAACGCCTGATGCGGCGAAAATCGCAACAGAGAAGCTCAA
AGAAATGGGCCCGTTCAAAAAATCGGAACTCTCTATGGTCGCGGTCTTTTTGCTCGTCCTTTTTTTATGGATTTTCGGAG
GCAACCTGAATATTGACGCGACAACAACGGCATTGATCGGTTTAGCCGTCCTGCTTTTGACACAGGTTCTGACGTGGGAC
GATATTAAGAAAGAGCAAGGCGCATGGGATACGCTGACATGGTTTGCCGCGCTCGTCATGCTGGCGAGCTTCTTGAATGA
ACTCGGCATGGTCGGCTGGTTCAGCGATGCGATGAAATCGTATGTTTCAGGATTCTCCTGGATCAGCGCATTTCTCATTT
TAATTGCGGTTTACTATTATTCGCATTATTTCTTTGCGAGTGCAACCGCTCATATCAGCGCGATGTATTCGGCCTTTCTC
GCCGTGATTATCGCTGCAGGCGCTCCGCCGCTTCTCGCAGCATTAAGCCTTGCGTTTTTCAGCAACCTTTTTGCTTCGAC
AACGCATTACGGAGCGGGAGCGGCTCCCGTCTTTTTCGGGGCCGGCTATATTCCGCAAAGCAAATGGTGGTCAATCGGCT
TCATCTTGTCGATTGTTCATATTGTGACATGGCTTGTTGTCGGAGGGTTATGGTGGAAATTACTGGGCATATGGTAA

Upstream 100 bases:

>100_bases
GCCGACATGGTCGGCAGTTTTTTTAATAAAATAAAATTTGAATGATAGCTTGAAAGGAGTAGAATAATAAACGAAAACGA
TATGAATAGGAGGAAAAGCT

Downstream 100 bases:

>100_bases
AATGAAGGGCGGGAGAGATCCTGTCCTTCTTTTTATGTTCATATCGACGCTGTTGATTTGAAATTCCACGATTTGTAGTC
TAGAAAGGGTTTGTTTAAAT

Product: YflS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MAKEKDAEQKSALNIIPLAVTILVGAVIWFIPSPAGLDPKAWHLFAIFVATIIGFISKPLPMGAIAVFALAITALTGTLS
IEETLSGFGNKTIWLIVIAFFISRGFIKTGLGARIAYLFVRMFGKKTLGLSYSLLVSDLILSPAIPSNTARAGGIIFPII
RSLSETFQSTPKDGTERRIGAFLLKVGFQGNLITSAMFLTAMAANPLIAKLAHDVAGVNLTWTSWAVAAIVPGLVSLIIT
PLVIYKLYPPEVKKTPDAAKIATEKLKEMGPFKKSELSMVAVFLLVLFLWIFGGNLNIDATTTALIGLAVLLLTQVLTWD
DIKKEQGAWDTLTWFAALVMLASFLNELGMVGWFSDAMKSYVSGFSWISAFLILIAVYYYSHYFFASATAHISAMYSAFL
AVIIAAGAPPLLAALSLAFFSNLFASTTHYGAGAAPVFFGAGYIPQSKWWSIGFILSIVHIVTWLVVGGLWWKLLGIW

Sequences:

>Translated_478_residues
MAKEKDAEQKSALNIIPLAVTILVGAVIWFIPSPAGLDPKAWHLFAIFVATIIGFISKPLPMGAIAVFALAITALTGTLS
IEETLSGFGNKTIWLIVIAFFISRGFIKTGLGARIAYLFVRMFGKKTLGLSYSLLVSDLILSPAIPSNTARAGGIIFPII
RSLSETFQSTPKDGTERRIGAFLLKVGFQGNLITSAMFLTAMAANPLIAKLAHDVAGVNLTWTSWAVAAIVPGLVSLIIT
PLVIYKLYPPEVKKTPDAAKIATEKLKEMGPFKKSELSMVAVFLLVLFLWIFGGNLNIDATTTALIGLAVLLLTQVLTWD
DIKKEQGAWDTLTWFAALVMLASFLNELGMVGWFSDAMKSYVSGFSWISAFLILIAVYYYSHYFFASATAHISAMYSAFL
AVIIAAGAPPLLAALSLAFFSNLFASTTHYGAGAAPVFFGAGYIPQSKWWSIGFILSIVHIVTWLVVGGLWWKLLGIW
>Mature_477_residues
AKEKDAEQKSALNIIPLAVTILVGAVIWFIPSPAGLDPKAWHLFAIFVATIIGFISKPLPMGAIAVFALAITALTGTLSI
EETLSGFGNKTIWLIVIAFFISRGFIKTGLGARIAYLFVRMFGKKTLGLSYSLLVSDLILSPAIPSNTARAGGIIFPIIR
SLSETFQSTPKDGTERRIGAFLLKVGFQGNLITSAMFLTAMAANPLIAKLAHDVAGVNLTWTSWAVAAIVPGLVSLIITP
LVIYKLYPPEVKKTPDAAKIATEKLKEMGPFKKSELSMVAVFLLVLFLWIFGGNLNIDATTTALIGLAVLLLTQVLTWDD
IKKEQGAWDTLTWFAALVMLASFLNELGMVGWFSDAMKSYVSGFSWISAFLILIAVYYYSHYFFASATAHISAMYSAFLA
VIIAAGAPPLLAALSLAFFSNLFASTTHYGAGAAPVFFGAGYIPQSKWWSIGFILSIVHIVTWLVVGGLWWKLLGIW

Specific function: Might be a malate transporter [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family [H]

Homologues:

Organism=Escherichia coli, GI1786986, Length=461, Percent_Identity=39.6963123644252, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI1786829, Length=484, Percent_Identity=34.297520661157, Blast_Score=301, Evalue=5e-83,
Organism=Escherichia coli, GI1789444, Length=474, Percent_Identity=36.0759493670886, Blast_Score=294, Evalue=7e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 51695; Mature: 51564

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKDAEQKSALNIIPLAVTILVGAVIWFIPSPAGLDPKAWHLFAIFVATIIGFISKPL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PMGAIAVFALAITALTGTLSIEETLSGFGNKTIWLIVIAFFISRGFIKTGLGARIAYLFV
CHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHCCHHHHCHHHHHHHHHH
RMFGKKTLGLSYSLLVSDLILSPAIPSNTARAGGIIFPIIRSLSETFQSTPKDGTERRIG
HHHCCHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
AFLLKVGFQGNLITSAMFLTAMAANPLIAKLAHDVAGVNLTWTSWAVAAIVPGLVSLIIT
HHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHH
PLVIYKLYPPEVKKTPDAAKIATEKLKEMGPFKKSELSMVAVFLLVLFLWIFGGNLNIDA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCH
TTTALIGLAVLLLTQVLTWDDIKKEQGAWDTLTWFAALVMLASFLNELGMVGWFSDAMKS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YVSGFSWISAFLILIAVYYYSHYFFASATAHISAMYSAFLAVIIAAGAPPLLAALSLAFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SNLFASTTHYGAGAAPVFFGAGYIPQSKWWSIGFILSIVHIVTWLVVGGLWWKLLGIW
HHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKEKDAEQKSALNIIPLAVTILVGAVIWFIPSPAGLDPKAWHLFAIFVATIIGFISKPL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PMGAIAVFALAITALTGTLSIEETLSGFGNKTIWLIVIAFFISRGFIKTGLGARIAYLFV
CHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHCCHHHHCHHHHHHHHHH
RMFGKKTLGLSYSLLVSDLILSPAIPSNTARAGGIIFPIIRSLSETFQSTPKDGTERRIG
HHHCCHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
AFLLKVGFQGNLITSAMFLTAMAANPLIAKLAHDVAGVNLTWTSWAVAAIVPGLVSLIIT
HHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHH
PLVIYKLYPPEVKKTPDAAKIATEKLKEMGPFKKSELSMVAVFLLVLFLWIFGGNLNIDA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCH
TTTALIGLAVLLLTQVLTWDDIKKEQGAWDTLTWFAALVMLASFLNELGMVGWFSDAMKS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YVSGFSWISAFLILIAVYYYSHYFFASATAHISAMYSAFLAVIIAAGAPPLLAALSLAFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SNLFASTTHYGAGAAPVFFGAGYIPQSKWWSIGFILSIVHIVTWLVVGGLWWKLLGIW
HHHHHHHCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 12949159 [H]