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The map label for this gene is cotA [H]

Identifier: 52784481

GI number: 52784481

Start: 681421

End: 682962

Strand: Reverse

Name: cotA [H]

Synonym: BLi00679

Alternate gene names: 52784481

Gene position: 682962-681421 (Counterclockwise)

Preceding gene: 52784483

Following gene: 52784480

Centisome position: 16.17

GC content: 48.7

Gene sequence:

>1542_bases
ATGAAACTTGAAAAATTCGTTGACCGGCTCCCCATTCCGCAAGTGCTTCAACCCCAAAGCAAAAGCAAGGAAATGACCTA
TTATGAAGTCACCATGAAAGAATTTCAGCAGCAGCTTCACCGCGATCTGCCGCCGACTCGGCTGTTTGGATATAACGGAG
TTTATCCCGGCCCTACCTTCGAAGTGCAGAAACACGAAAAAGTCGCAGTCAAGTGGTTAAATAAGCTTCCGGATCGCCAT
TTTCTCCCCGTCGACCATACGCTTCACGATGACGGCCATCACGAACATGAAGTGAAGACGGTCGTTCATTTGCACGGAGG
CTGTACGCCTGCTGACAGCGACGGATATCCTGAGGCTTGGTACACAAAAGACTTCCATGCAAAAGGCCCTTTCTTTGAAA
GGGAGGTGTATGAATATCCGAATGAGCAGGATGCTACAGCTCTTTGGTATCATGACCATGCAATGGCCATCACAAGGCTG
AATGTATATGCGGGGCTTGTCGGTTTATATTTTATTCGCGACAGGGAAGAGCGTTCATTGAACTTGCCGAAGGGAGAATA
TGAAATCCCGCTTTTGATTCAGGATAAATCATTTCATGAAGATGGTTCATTGTTTTATCCGCGGCAGCCTGACAACCCTT
CGCCGGATCTTCCGGACCCGTCGATTGTTCCGGCTTTTTGCGGTGATACCATTTTAGTCAACGGCAAGGTATGGCCTTTC
GCTGAACTGGAACCCCGAAAATACCGTTTTCGGATACTGAACGCCTCCAATACGAGAATCTTTGAGCTGTATTTCGATCA
TGACATCACATGTCATCAAATCGGCACGGACGGCGGTCTTCTGCAGCATCCGGTCAAAGTCAATGAACTGGTGATCGCGC
CGGCTGAAAGGTGCGATATCATCGTTGATTTTTCACGAGCAGAAGGAAAAACCGTGACACTGAAAAAACGGATCGGCTGC
GGCGGACAAGACGCAGATCCCGATACTGATGCCGACATCATGCAATTCCGCATCTCAAAACCTTTGAAGCAAAAAGATAC
AAGTTCATTGCCGAGAATATTGAGAAAGCGCCCATTTTACCGGAGACACAAGATCAATGCCCTCAGAAATCTGTCATTGG
GCGCGGCCGTTGACCAATATGGAAGACCTGTTCTGCTTTTAAACAACACAAAGTGGCATGAACCGGTAACCGAAACACCC
GCACTCGGCAGCACTGAGATCTGGTCGATCATCAATGCCGGAAGGGCGATCCATCCGATCCATTTACATCTTGTTCAATT
TATGATTCTCGACCACCGGCCGTTTGATATCGAGCGGTATCAGGAAAACGGAGAACTTGTTTTTACCGGTCCGGCAGTTC
CTCCGGCACCGAATGAAAAGGGGCTGAAAGACACCGTCAAAGTACCCCCGGGTTCAGTGACGCGCATTATCGCCACCTTT
GCGCCGTACAGCGGCAGATATGTTTGGCACTGCCACATCCTTGAGCACGAAGATTACGATATGATGCGCCCTCTTGAAGT
GACAGATGTTCGTCATCAATAA

Upstream 100 bases:

>100_bases
ACATACTGCGTTTCTTATTCTCATCATGAGGAACCAGACCATGCAACCCGTCTTATAATGTAATGATCTTTTCATCAGAA
CAAAAGAGGACAGGTGAAAC

Downstream 100 bases:

>100_bases
GAAAAAGCCTCCGCATTTGGAGGCTTTTAAAAAAGCACAAATCATCAAAAAAACCATCGCTTGATCCTGTACAATAGGAT
CATCAAATCGTAAAGGCTGT

Product: CotA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MKLEKFVDRLPIPQVLQPQSKSKEMTYYEVTMKEFQQQLHRDLPPTRLFGYNGVYPGPTFEVQKHEKVAVKWLNKLPDRH
FLPVDHTLHDDGHHEHEVKTVVHLHGGCTPADSDGYPEAWYTKDFHAKGPFFEREVYEYPNEQDATALWYHDHAMAITRL
NVYAGLVGLYFIRDREERSLNLPKGEYEIPLLIQDKSFHEDGSLFYPRQPDNPSPDLPDPSIVPAFCGDTILVNGKVWPF
AELEPRKYRFRILNASNTRIFELYFDHDITCHQIGTDGGLLQHPVKVNELVIAPAERCDIIVDFSRAEGKTVTLKKRIGC
GGQDADPDTDADIMQFRISKPLKQKDTSSLPRILRKRPFYRRHKINALRNLSLGAAVDQYGRPVLLLNNTKWHEPVTETP
ALGSTEIWSIINAGRAIHPIHLHLVQFMILDHRPFDIERYQENGELVFTGPAVPPAPNEKGLKDTVKVPPGSVTRIIATF
APYSGRYVWHCHILEHEDYDMMRPLEVTDVRHQ

Sequences:

>Translated_513_residues
MKLEKFVDRLPIPQVLQPQSKSKEMTYYEVTMKEFQQQLHRDLPPTRLFGYNGVYPGPTFEVQKHEKVAVKWLNKLPDRH
FLPVDHTLHDDGHHEHEVKTVVHLHGGCTPADSDGYPEAWYTKDFHAKGPFFEREVYEYPNEQDATALWYHDHAMAITRL
NVYAGLVGLYFIRDREERSLNLPKGEYEIPLLIQDKSFHEDGSLFYPRQPDNPSPDLPDPSIVPAFCGDTILVNGKVWPF
AELEPRKYRFRILNASNTRIFELYFDHDITCHQIGTDGGLLQHPVKVNELVIAPAERCDIIVDFSRAEGKTVTLKKRIGC
GGQDADPDTDADIMQFRISKPLKQKDTSSLPRILRKRPFYRRHKINALRNLSLGAAVDQYGRPVLLLNNTKWHEPVTETP
ALGSTEIWSIINAGRAIHPIHLHLVQFMILDHRPFDIERYQENGELVFTGPAVPPAPNEKGLKDTVKVPPGSVTRIIATF
APYSGRYVWHCHILEHEDYDMMRPLEVTDVRHQ
>Mature_513_residues
MKLEKFVDRLPIPQVLQPQSKSKEMTYYEVTMKEFQQQLHRDLPPTRLFGYNGVYPGPTFEVQKHEKVAVKWLNKLPDRH
FLPVDHTLHDDGHHEHEVKTVVHLHGGCTPADSDGYPEAWYTKDFHAKGPFFEREVYEYPNEQDATALWYHDHAMAITRL
NVYAGLVGLYFIRDREERSLNLPKGEYEIPLLIQDKSFHEDGSLFYPRQPDNPSPDLPDPSIVPAFCGDTILVNGKVWPF
AELEPRKYRFRILNASNTRIFELYFDHDITCHQIGTDGGLLQHPVKVNELVIAPAERCDIIVDFSRAEGKTVTLKKRIGC
GGQDADPDTDADIMQFRISKPLKQKDTSSLPRILRKRPFYRRHKINALRNLSLGAAVDQYGRPVLLLNNTKWHEPVTETP
ALGSTEIWSIINAGRAIHPIHLHLVQFMILDHRPFDIERYQENGELVFTGPAVPPAPNEKGLKDTVKVPPGSVTRIIATF
APYSGRYVWHCHILEHEDYDMMRPLEVTDVRHQ

Specific function: Involved in brown pigmentation during sporogenesis [H]

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To S.antibioticus phenoxazinone synthase (phsA) [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=550, Percent_Identity=27.0909090909091, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1789394, Length=471, Percent_Identity=27.8131634819533, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR008972 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 59089; Mature: 59089

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEKFVDRLPIPQVLQPQSKSKEMTYYEVTMKEFQQQLHRDLPPTRLFGYNGVYPGPTF
CCHHHHHHHCCCCHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCEECCCCCCCCCCE
EVQKHEKVAVKWLNKLPDRHFLPVDHTLHDDGHHEHEVKTVVHLHGGCTPADSDGYPEAW
EECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCC
YTKDFHAKGPFFEREVYEYPNEQDATALWYHDHAMAITRLNVYAGLVGLYFIRDREERSL
CCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCEEEEEHHHHHHHHHHHEEEECCCCCCC
NLPKGEYEIPLLIQDKSFHEDGSLFYPRQPDNPSPDLPDPSIVPAFCGDTILVNGKVWPF
CCCCCCEEEEEEEECCCCCCCCCEECCCCCCCCCCCCCCCCEEHHHCCCEEEECCCCCCC
AELEPRKYRFRILNASNTRIFELYFDHDITCHQIGTDGGLLQHPVKVNELVIAPAERCDI
CCCCCCEEEEEEEECCCCEEEEEEECCCCEEEEECCCCCCEECCCEECEEEEECHHHCEE
IVDFSRAEGKTVTLKKRIGCGGQDADPDTDADIMQFRISKPLKQKDTSSLPRILRKRPFY
EEEECCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHCCHHHHHHHHHHCCCH
RRHKINALRNLSLGAAVDQYGRPVLLLNNTKWHEPVTETPALGSTEIWSIINAGRAIHPI
HHHHHHHHHCCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCEECHH
HLHLVQFMILDHRPFDIERYQENGELVFTGPAVPPAPNEKGLKDTVKVPPGSVTRIIATF
HHHHHHHHHHCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCEECCCCCHHHHHHHHH
APYSGRYVWHCHILEHEDYDMMRPLEVTDVRHQ
CCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLEKFVDRLPIPQVLQPQSKSKEMTYYEVTMKEFQQQLHRDLPPTRLFGYNGVYPGPTF
CCHHHHHHHCCCCHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCEECCCCCCCCCCE
EVQKHEKVAVKWLNKLPDRHFLPVDHTLHDDGHHEHEVKTVVHLHGGCTPADSDGYPEAW
EECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCC
YTKDFHAKGPFFEREVYEYPNEQDATALWYHDHAMAITRLNVYAGLVGLYFIRDREERSL
CCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCEEEEEHHHHHHHHHHHEEEECCCCCCC
NLPKGEYEIPLLIQDKSFHEDGSLFYPRQPDNPSPDLPDPSIVPAFCGDTILVNGKVWPF
CCCCCCEEEEEEEECCCCCCCCCEECCCCCCCCCCCCCCCCEEHHHCCCEEEECCCCCCC
AELEPRKYRFRILNASNTRIFELYFDHDITCHQIGTDGGLLQHPVKVNELVIAPAERCDI
CCCCCCEEEEEEEECCCCEEEEEEECCCCEEEEECCCCCCEECCCEECEEEEECHHHCEE
IVDFSRAEGKTVTLKKRIGCGGQDADPDTDADIMQFRISKPLKQKDTSSLPRILRKRPFY
EEEECCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHCCHHHHHHHHHHCCCH
RRHKINALRNLSLGAAVDQYGRPVLLLNNTKWHEPVTETPALGSTEIWSIINAGRAIHPI
HHHHHHHHHCCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCEECHH
HLHLVQFMILDHRPFDIERYQENGELVFTGPAVPPAPNEKGLKDTVKVPPGSVTRIIATF
HHHHHHHHHHCCCCCCHHHHCCCCCEEEECCCCCCCCCCCCCCCEECCCCCHHHHHHHHH
APYSGRYVWHCHILEHEDYDMMRPLEVTDVRHQ
CCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969499; 9455482; 9384377; 2821284; 3135411 [H]