Click here to switch to the map view.

The map label for this gene is tyrZ

Identifier: 52784468

GI number: 52784468

Start: 671278

End: 672531

Strand: Reverse

Name: tyrZ

Synonym: BLi00666

Alternate gene names: 52784468

Gene position: 672531-671278 (Counterclockwise)

Preceding gene: 52784469

Following gene: 52784453

Centisome position: 15.93

GC content: 48.88

Gene sequence:

>1254_bases
ATGCAGCAACAACAATTTGATCAACTGACAACTGCCGAAAAGCGGGAAGTCGAAAGGCAGCTTCACTTATATATGGACGG
AGCGGAGGAGGTCATTCCCCCGGAGGAACTGCGGGCAAAGCTGGCCAAATCGATTTTGACCGGCGAACCGCTGAAAATTA
AGCTCGGCCTCGATCCGTCTGCGCCTGACGTACATTTGGGCCACACCGTCGTCTTGAATAAGCTTCGTCAGTTTCAGGAA
AACGGACATATCGTCCAGCTCCTGATCGGCGATTTCACGGGGAAAATCGGTGATCCAACAGGAAAATCCGCCGCCAGGAA
ACAGCTGACGGACGAAGAGGTCCGCCGCAATGCCGAAACATACTTTCAACAGTTTGGAAAAGTGCTTGATCAGAACAAGG
TCGAGCTTCACTACAACTCAAAATGGCTTAAACGATTAACCTTGGAAGACGTCATTGAGCTCGCGGGCAAAATTACCGTC
GCCCGTTTAATGGAACGCGATGATTTTGAAGAAAGAATCGCCATGCAAAAGCCGATTTCGCTTCATGAGTTCTTTTACCC
GTTGATGCAGGGGTATGATTCAGTCGTTCTGGACAGCGACATCGAACTGGGCGGAACGGATCAGCATTTTAACGTGCTCA
TGGGCAGACACTTTCAGGAAAAGTACGGAAAAGAAAAACAGGTCGTCATTCTCATGCCGCTGCTTGAAGGGCTTGACGGC
ATTGAAAAAATGTCTAAATCAAAGAACAATTACATCGGCATCGATGAAGACCCTCATGAAATGTTCGGGAAAACGATGTC
TCTGCCGGACAGTTTGATGAAAAAGTATATCCATCTTGCGACCGACTTGGAGGCGGAAGAAAAAAAACGTCTTGTCAAGG
ATTTGGAAACGGGGGCCGTTCATCCGAGAGACGCGAAGATGCTTCTCGGGAAAACCATTGTCAACATGTATCATGGAAGC
AAGGCGGCAGAAGCGGCCGAACAGCAGTTTAAGAAAGTTTTTCAGCAAAACAGCGCGCCGGACAATATAACAGCCGTACA
CTGGCAAGGTGGCGGAACGGTGCCGGTCACCGATTTATTGGTGACGCTCGAACTCCTTCCTTCCAAAAGTGAAGCCCGCC
GCATGATCCAAAACGGCGGCGTTAAAATCAACGGCGCCAAAGCGGAATCGATTCATGCGGACATTGCACTCGAACACGGC
ATGATCATCCAAGTCGGCAAACGAAAATTTGTCAAATTGCAAATGCACCAATAA

Upstream 100 bases:

>100_bases
GAAGATGAAGGTCTTCAATCAGGGTGGTACCGCGTGCATTTGAGCCACGTCCCTTATCGGGATGAGGTTCTTTTTTTATA
CAAAAAAAGGAGGAGTTATC

Downstream 100 bases:

>100_bases
ACAAACAGCCTAATCATCCTTTTAAAGGGATCGATTAGGCTGTTTTTCTTGAAAAACGCAGGCGATAGACTCTGCAACAG
GACTGTATCCAACCGCCTGA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase 2; TyrRS 2

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MQQQQFDQLTTAEKREVERQLHLYMDGAEEVIPPEELRAKLAKSILTGEPLKIKLGLDPSAPDVHLGHTVVLNKLRQFQE
NGHIVQLLIGDFTGKIGDPTGKSAARKQLTDEEVRRNAETYFQQFGKVLDQNKVELHYNSKWLKRLTLEDVIELAGKITV
ARLMERDDFEERIAMQKPISLHEFFYPLMQGYDSVVLDSDIELGGTDQHFNVLMGRHFQEKYGKEKQVVILMPLLEGLDG
IEKMSKSKNNYIGIDEDPHEMFGKTMSLPDSLMKKYIHLATDLEAEEKKRLVKDLETGAVHPRDAKMLLGKTIVNMYHGS
KAAEAAEQQFKKVFQQNSAPDNITAVHWQGGGTVPVTDLLVTLELLPSKSEARRMIQNGGVKINGAKAESIHADIALEHG
MIIQVGKRKFVKLQMHQ

Sequences:

>Translated_417_residues
MQQQQFDQLTTAEKREVERQLHLYMDGAEEVIPPEELRAKLAKSILTGEPLKIKLGLDPSAPDVHLGHTVVLNKLRQFQE
NGHIVQLLIGDFTGKIGDPTGKSAARKQLTDEEVRRNAETYFQQFGKVLDQNKVELHYNSKWLKRLTLEDVIELAGKITV
ARLMERDDFEERIAMQKPISLHEFFYPLMQGYDSVVLDSDIELGGTDQHFNVLMGRHFQEKYGKEKQVVILMPLLEGLDG
IEKMSKSKNNYIGIDEDPHEMFGKTMSLPDSLMKKYIHLATDLEAEEKKRLVKDLETGAVHPRDAKMLLGKTIVNMYHGS
KAAEAAEQQFKKVFQQNSAPDNITAVHWQGGGTVPVTDLLVTLELLPSKSEARRMIQNGGVKINGAKAESIHADIALEHG
MIIQVGKRKFVKLQMHQ
>Mature_417_residues
MQQQQFDQLTTAEKREVERQLHLYMDGAEEVIPPEELRAKLAKSILTGEPLKIKLGLDPSAPDVHLGHTVVLNKLRQFQE
NGHIVQLLIGDFTGKIGDPTGKSAARKQLTDEEVRRNAETYFQQFGKVLDQNKVELHYNSKWLKRLTLEDVIELAGKITV
ARLMERDDFEERIAMQKPISLHEFFYPLMQGYDSVVLDSDIELGGTDQHFNVLMGRHFQEKYGKEKQVVILMPLLEGLDG
IEKMSKSKNNYIGIDEDPHEMFGKTMSLPDSLMKKYIHLATDLEAEEKKRLVKDLETGAVHPRDAKMLLGKTIVNMYHGS
KAAEAAEQQFKKVFQQNSAPDNITAVHWQGGGTVPVTDLLVTLELLPSKSEARRMIQNGGVKINGAKAESIHADIALEHG
MIIQVGKRKFVKLQMHQ

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=412, Percent_Identity=27.6699029126214, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1787925, Length=382, Percent_Identity=26.7015706806283, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25145802, Length=414, Percent_Identity=24.1545893719807, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325160, Length=444, Percent_Identity=25.6756756756757, Blast_Score=110, Evalue=6e-25,
Organism=Drosophila melanogaster, GI20130383, Length=411, Percent_Identity=24.5742092457421, Blast_Score=125, Evalue=7e-29,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY2_BACLD (Q65MW0)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077893.1
- RefSeq:   YP_090297.1
- ProteinModelPortal:   Q65MW0
- SMR:   Q65MW0
- STRING:   Q65MW0
- EnsemblBacteria:   EBBACT00000054858
- EnsemblBacteria:   EBBACT00000059664
- GeneID:   3031036
- GeneID:   3100653
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00666
- KEGG:   bli:BL00484
- NMPDR:   fig|279010.5.peg.925
- eggNOG:   COG0162
- GeneTree:   EBGT00050000000599
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- ProtClustDB:   PRK13354
- BioCyc:   BLIC279010-1:BLI00666-MONOMER
- BioCyc:   BLIC279010:BL00484-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 47193; Mature: 47193

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 246-246

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQQFDQLTTAEKREVERQLHLYMDGAEEVIPPEELRAKLAKSILTGEPLKIKLGLDPS
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCC
APDVHLGHTVVLNKLRQFQENGHIVQLLIGDFTGKIGDPTGKSAARKQLTDEEVRRNAET
CCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHH
YFQQFGKVLDQNKVELHYNSKWLKRLTLEDVIELAGKITVARLMERDDFEERIAMQKPIS
HHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCH
LHEFFYPLMQGYDSVVLDSDIELGGTDQHFNVLMGRHFQEKYGKEKQVVILMPLLEGLDG
HHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCHH
IEKMSKSKNNYIGIDEDPHEMFGKTMSLPDSLMKKYIHLATDLEAEEKKRLVKDLETGAV
HHHHHHCCCCEECCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
HPRDAKMLLGKTIVNMYHGSKAAEAAEQQFKKVFQQNSAPDNITAVHWQGGGTVPVTDLL
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHH
VTLELLPSKSEARRMIQNGGVKINGAKAESIHADIALEHGMIIQVGKRKFVKLQMHQ
HHHHHCCCHHHHHHHHHHCCEEEECCCCCHHHHHEEECCCEEEEECCCEEEEEEECC
>Mature Secondary Structure
MQQQQFDQLTTAEKREVERQLHLYMDGAEEVIPPEELRAKLAKSILTGEPLKIKLGLDPS
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCC
APDVHLGHTVVLNKLRQFQENGHIVQLLIGDFTGKIGDPTGKSAARKQLTDEEVRRNAET
CCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHH
YFQQFGKVLDQNKVELHYNSKWLKRLTLEDVIELAGKITVARLMERDDFEERIAMQKPIS
HHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCH
LHEFFYPLMQGYDSVVLDSDIELGGTDQHFNVLMGRHFQEKYGKEKQVVILMPLLEGLDG
HHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCHH
IEKMSKSKNNYIGIDEDPHEMFGKTMSLPDSLMKKYIHLATDLEAEEKKRLVKDLETGAV
HHHHHHCCCCEECCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
HPRDAKMLLGKTIVNMYHGSKAAEAAEQQFKKVFQQNSAPDNITAVHWQGGGTVPVTDLL
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHH
VTLELLPSKSEARRMIQNGGVKINGAKAESIHADIALEHGMIIQVGKRKFVKLQMHQ
HHHHHCCCHHHHHHHHHHCCEEEECCCCCHHHHHEEECCCEEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA