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The map label for this gene is ansB [H]

Identifier: 52784348

GI number: 52784348

Start: 533953

End: 535374

Strand: Reverse

Name: ansB [H]

Synonym: BLi00528

Alternate gene names: 52784348

Gene position: 535374-533953 (Counterclockwise)

Preceding gene: 52784350

Following gene: 52784347

Centisome position: 12.68

GC content: 49.51

Gene sequence:

>1422_bases
ATGGATACGAAAGCAACCAGGATCGAGAAGGATTTTCTAGGTGAAAAAGAAGTTCCCGTTGACGCCTATTACGGCGTACA
AACCATGAGAGCCGTGGAGAACTTTCCGATTACAGGCTATCAGATTGATGAAGAGCTGATTAAAGCTTTGGCAATGGTCA
AAAAAGCGGCGGCTCTCGCCAACATGGATATTAAACGTTTATATAAAGAGCTTGGCGAAGTCATCGTAAAAGCTGCCGGC
GAAATAGCGGAAGGGCTCTGGCACGAGCATTTTATTGTCGATCCCATTCAAGGCGGCGCCGGCACTTCAATGAATATGAA
TACGAATGAAGTTATCGCAAACAGAGGTTTAGAACTTTTAGGGAAAGAAAAAGGGGATTACTTCCATTTAAGTCCGAACA
CACATGTCAATATGGCCCAATCAACGAATGATGTGTTTCCGACAGCCATTCATCTGGCCACATTGAATCACTTAAAACGG
CTCTTATCGACGATGGAGCATATGTTGGATGCCTTCCGCAAAAAAGCGGCGGAATTCGATTCAGTCATTAAAATGGGCCG
CACCCATCTTCAGGATGCCGTCCCGATCAGGCTCGGCCAGGAATTCGAAGCCTACTCCCGGGTGATCGAACGCGATATTC
AGCGGATCAGGCAGTCGCAGCATCATCTCTATGAAGTCAATATGGGGGCCACGGCCGTCGGCACAGGTTTGAACGCCGAT
CCGAGATATATTCAAAACGTTGTCGCGCACCTCTCAGATATTAGCGGCTATCAGCTGACCGGCGCAGACCATCTTGTCGA
TGCCACACAAAACACGGATGCCTACACAGAAGTATCGGCTGCTTTGAAAGTCGGCATGATGAACATGTCCAAGATCGCCA
ACGATTTACGTTTAATGGCGTCTGGCCCGAGGGCAGGTCTAGCCGAGATCCGGCTGCCGCCCCGCCAGCCCGGCTCCTCC
ATCATGCCCGGCAAAGTAAATCCGGTGATGCCTGAGCTGATGAACCAGATCGCATTTCAGGTGATCGGCAACGATAATAC
CATCTGCCTTGCATCAGAGGCGGGTCAGCTTGAGTTAAACGTGATGGAGCCCGTGCTTGTCTTTAATTTGCTGCAGTCGA
TTAAAATCATGAACAACGGATTTCGCGTATTTACCGATTACTGCATCACCGGGATTGAAGCGAATGAGATCAGGCTGAAG
GAATTCGTTGAAAAAAGCGTAGGGACCATTACGGCTGTCAATCCGCACCTTGGCTATGAAGCCGCCGCACGAATTGCGAA
AGAAGCGATCGCCACAGGACGATCAGTGCGCGAGCTGTGTTTGCTCCATGATGTTTTAACAGAGGAAGAATTGGATCTCA
TTCTGGATCCTTATGAAATGACGCATCCGGGCATCGCAGGCGCTTCGCTTCTCGAGGAATAG

Upstream 100 bases:

>100_bases
CCCCATGTCGGAAGACGATTTTCCTTTTTTTAAAAGCAAATTAAAACATGGCGGATCGAGGCACAATACATCATAAGAAA
CAACCTACGGAGGTTTTGCC

Downstream 100 bases:

>100_bases
CCATCCGTCTTTCTCAATATGCTTTCAAGCGTTTATAGACGCCCGAGACGGGCTAAACATTTAATATAAATCAATTGAAA
GAGGGTGGAAAGACAATGTT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MDTKATRIEKDFLGEKEVPVDAYYGVQTMRAVENFPITGYQIDEELIKALAMVKKAAALANMDIKRLYKELGEVIVKAAG
EIAEGLWHEHFIVDPIQGGAGTSMNMNTNEVIANRGLELLGKEKGDYFHLSPNTHVNMAQSTNDVFPTAIHLATLNHLKR
LLSTMEHMLDAFRKKAAEFDSVIKMGRTHLQDAVPIRLGQEFEAYSRVIERDIQRIRQSQHHLYEVNMGATAVGTGLNAD
PRYIQNVVAHLSDISGYQLTGADHLVDATQNTDAYTEVSAALKVGMMNMSKIANDLRLMASGPRAGLAEIRLPPRQPGSS
IMPGKVNPVMPELMNQIAFQVIGNDNTICLASEAGQLELNVMEPVLVFNLLQSIKIMNNGFRVFTDYCITGIEANEIRLK
EFVEKSVGTITAVNPHLGYEAAARIAKEAIATGRSVRELCLLHDVLTEEELDLILDPYEMTHPGIAGASLLEE

Sequences:

>Translated_473_residues
MDTKATRIEKDFLGEKEVPVDAYYGVQTMRAVENFPITGYQIDEELIKALAMVKKAAALANMDIKRLYKELGEVIVKAAG
EIAEGLWHEHFIVDPIQGGAGTSMNMNTNEVIANRGLELLGKEKGDYFHLSPNTHVNMAQSTNDVFPTAIHLATLNHLKR
LLSTMEHMLDAFRKKAAEFDSVIKMGRTHLQDAVPIRLGQEFEAYSRVIERDIQRIRQSQHHLYEVNMGATAVGTGLNAD
PRYIQNVVAHLSDISGYQLTGADHLVDATQNTDAYTEVSAALKVGMMNMSKIANDLRLMASGPRAGLAEIRLPPRQPGSS
IMPGKVNPVMPELMNQIAFQVIGNDNTICLASEAGQLELNVMEPVLVFNLLQSIKIMNNGFRVFTDYCITGIEANEIRLK
EFVEKSVGTITAVNPHLGYEAAARIAKEAIATGRSVRELCLLHDVLTEEELDLILDPYEMTHPGIAGASLLEE
>Mature_473_residues
MDTKATRIEKDFLGEKEVPVDAYYGVQTMRAVENFPITGYQIDEELIKALAMVKKAAALANMDIKRLYKELGEVIVKAAG
EIAEGLWHEHFIVDPIQGGAGTSMNMNTNEVIANRGLELLGKEKGDYFHLSPNTHVNMAQSTNDVFPTAIHLATLNHLKR
LLSTMEHMLDAFRKKAAEFDSVIKMGRTHLQDAVPIRLGQEFEAYSRVIERDIQRIRQSQHHLYEVNMGATAVGTGLNAD
PRYIQNVVAHLSDISGYQLTGADHLVDATQNTDAYTEVSAALKVGMMNMSKIANDLRLMASGPRAGLAEIRLPPRQPGSS
IMPGKVNPVMPELMNQIAFQVIGNDNTICLASEAGQLELNVMEPVLVFNLLQSIKIMNNGFRVFTDYCITGIEANEIRLK
EFVEKSVGTITAVNPHLGYEAAARIAKEAIATGRSVRELCLLHDVLTEEELDLILDPYEMTHPGIAGASLLEE

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=460, Percent_Identity=41.9565217391304, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI87082375, Length=470, Percent_Identity=47.2340425531915, Blast_Score=425, Evalue=1e-120,
Organism=Escherichia coli, GI1787896, Length=462, Percent_Identity=40.6926406926407, Blast_Score=352, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=42.8260869565217, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=41.2844036697248, Blast_Score=268, Evalue=6e-72,
Organism=Saccharomyces cerevisiae, GI6324993, Length=460, Percent_Identity=41.7391304347826, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640179, Length=470, Percent_Identity=40.8510638297872, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24640177, Length=464, Percent_Identity=41.1637931034483, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=40.4761904761905, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=41.036717062635, Blast_Score=348, Evalue=6e-96,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=36.9330453563715, Blast_Score=319, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52208; Mature: 52208

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTKATRIEKDFLGEKEVPVDAYYGVQTMRAVENFPITGYQIDEELIKALAMVKKAAALA
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHH
NMDIKRLYKELGEVIVKAAGEIAEGLWHEHFIVDPIQGGAGTSMNMNTNEVIANRGLELL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCHHHHHHHCCHHHH
GKEKGDYFHLSPNTHVNMAQSTNDVFPTAIHLATLNHLKRLLSTMEHMLDAFRKKAAEFD
CCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVIKMGRTHLQDAVPIRLGQEFEAYSRVIERDIQRIRQSQHHLYEVNMGATAVGTGLNAD
HHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEECCCCCCC
PRYIQNVVAHLSDISGYQLTGADHLVDATQNTDAYTEVSAALKVGMMNMSKIANDLRLMA
HHHHHHHHHHHHHCCCCEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGPRAGLAEIRLPPRQPGSSIMPGKVNPVMPELMNQIAFQVIGNDNTICLASEAGQLELN
CCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCEEEEECCCCCEEEE
VMEPVLVFNLLQSIKIMNNGFRVFTDYCITGIEANEIRLKEFVEKSVGTITAVNPHLGYE
HHHHHHHHHHHHHHHHHCCCHHEEHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCHH
AAARIAKEAIATGRSVRELCLLHDVLTEEELDLILDPYEMTHPGIAGASLLEE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHEECCHHHCCCCCCCHHHHCC
>Mature Secondary Structure
MDTKATRIEKDFLGEKEVPVDAYYGVQTMRAVENFPITGYQIDEELIKALAMVKKAAALA
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHH
NMDIKRLYKELGEVIVKAAGEIAEGLWHEHFIVDPIQGGAGTSMNMNTNEVIANRGLELL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCHHHHHHHCCHHHH
GKEKGDYFHLSPNTHVNMAQSTNDVFPTAIHLATLNHLKRLLSTMEHMLDAFRKKAAEFD
CCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVIKMGRTHLQDAVPIRLGQEFEAYSRVIERDIQRIRQSQHHLYEVNMGATAVGTGLNAD
HHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEECCCCCCC
PRYIQNVVAHLSDISGYQLTGADHLVDATQNTDAYTEVSAALKVGMMNMSKIANDLRLMA
HHHHHHHHHHHHHCCCCEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
SGPRAGLAEIRLPPRQPGSSIMPGKVNPVMPELMNQIAFQVIGNDNTICLASEAGQLELN
CCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCEEEEECCCCCEEEE
VMEPVLVFNLLQSIKIMNNGFRVFTDYCITGIEANEIRLKEFVEKSVGTITAVNPHLGYE
HHHHHHHHHHHHHHHHHCCCHHEEHHHHHCCCCCCHHHHHHHHHHHCCEEEEECCCCCHH
AAARIAKEAIATGRSVRELCLLHDVLTEEELDLILDPYEMTHPGIAGASLLEE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHEECCHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]