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The map label for this gene is yclM [H]

Identifier: 52784282

GI number: 52784282

Start: 464415

End: 465776

Strand: Reverse

Name: yclM [H]

Synonym: BLi00462

Alternate gene names: 52784282

Gene position: 465776-464415 (Counterclockwise)

Preceding gene: 52784287

Following gene: 52784281

Centisome position: 11.03

GC content: 51.03

Gene sequence:

>1362_bases
ATGAAAGTCGTTAAATTTGGAGGCAGCTCGCTCGCATCAGGAGCCCAGCTAGAGAAAGTATTTCAAATCGTCACTTCCGA
TCCGGAAAGGAGGGCCGTCGTCGTATCGGCACCCGGAAAACGGCATCCCGAGGATACGAAAGTAACTGACCTGTTAATCG
AATGCGCCCAACAGTACCTATTATCGAAAGAAGCGACCGGCCTGGTTGAGGCGATTGTCGGACGGTATGCCGATATCGCG
CTAGAGCTCGGGCTTGCCGATGACGTGATCAACCGGATCCGGGCAGACCTGCTTCAGCTGCTCGAAGGTGATAAAACAAA
TCCGGAACGGTTTATTGATGCGATCAAAGCTAGCGGCGAAGATAACAATGCCAAACTGGTAGCGGCCTACTTCAGGCACC
GTGGAGTTGAAGCACATTATGTCAGTCCAAAAGACGCCGGGCTGTTCGTGACGAGCGAACCCGGCAACGCACAAGTGCTG
CCCGAGTCCTACGACAATCTGTTTCAGCTCAGACAGCAAAAGGGCATCATTATCTTTCCCGGCTTCTTCGGATTCAGCCA
GGAAGGAGAAGTGATGACGTTTTCGCGCAGCGGCTCTGACATTACGGGCTCCATTTTAGCGAATGGATTGAAAGCCGAGT
TGTATGAAAACTTCACTGACGTTGACGCCGTTTATTCTGTCAACCCGGCGATCGTATCCAACCCGAAAGAAATCCGCGAG
CTGACATACCGGGAAATGAGAGAGCTGTCGTATGCCGGCTTTTCCGTCTTTCATGATGAAGCATTGATCCCTGCTTTCCG
GGCGCGCATACCGGTTCAGATCAAAAATACAAACAACCCGGACGCAGAAGGGACAAGAGTTGTCGCCACACGCGACAACA
CGAACGGACCTGTCGTCGGGATCGCCGGCGACAGCGGATTTTGCAGCATTTACATCAGCAAATATTTGATGAACCGAGAA
ATCGGCTTCGGCCGCCGCGTCCTGCAAATCCTTGAAGATGAAGGGCTGACATATGAACACATTCCGTCCGGCATTGACGA
TATTACGATCATTTTAAGGCAGAATCAAATGGATCAAGCAGCAGAAAAACGGATGATCAAACGGATCAAGGAAGAGTTGG
ATGCTGATGAAGTAGCGATTGAACACAATATTGCCCTGATCATGGTCGTCGGCGAAGCCATGCGCCATAACGTCGGAACG
ACGGCAAGAGCGTCAAAAGCATTGTCTGAAGCCGGCGTCAACATCGAAATGATCAACCAGGGCTCCTCGGAAGTCAGCAT
GATGTTCGGCGTTAAGGAAGCTCAGGAAAAACAAGCGGTCCGCGCATTATACCAGGAGTTTTTCGCAAAAGTTCTCGTTT
AA

Upstream 100 bases:

>100_bases
TTGTTCTTTCCTGAAAGAAAAACCTTGATTCTTCTTGTAAAAAGCGCAATAATTAAAATTAAATTTATTTCGAAAATTTA
AACTTTAGGAGATGTAACAA

Downstream 100 bases:

>100_bases
AAAAGCCCCTTTTTTATGGGGTTTTTTTGTTGTCAGAAAATGTCCCCTTCCCTTAAATTGCCTGCCCTAAATTGATTCAC
AAAACCGTTTTGAAAGCCGA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKVVKFGGSSLASGAQLEKVFQIVTSDPERRAVVVSAPGKRHPEDTKVTDLLIECAQQYLLSKEATGLVEAIVGRYADIA
LELGLADDVINRIRADLLQLLEGDKTNPERFIDAIKASGEDNNAKLVAAYFRHRGVEAHYVSPKDAGLFVTSEPGNAQVL
PESYDNLFQLRQQKGIIIFPGFFGFSQEGEVMTFSRSGSDITGSILANGLKAELYENFTDVDAVYSVNPAIVSNPKEIRE
LTYREMRELSYAGFSVFHDEALIPAFRARIPVQIKNTNNPDAEGTRVVATRDNTNGPVVGIAGDSGFCSIYISKYLMNRE
IGFGRRVLQILEDEGLTYEHIPSGIDDITIILRQNQMDQAAEKRMIKRIKEELDADEVAIEHNIALIMVVGEAMRHNVGT
TARASKALSEAGVNIEMINQGSSEVSMMFGVKEAQEKQAVRALYQEFFAKVLV

Sequences:

>Translated_453_residues
MKVVKFGGSSLASGAQLEKVFQIVTSDPERRAVVVSAPGKRHPEDTKVTDLLIECAQQYLLSKEATGLVEAIVGRYADIA
LELGLADDVINRIRADLLQLLEGDKTNPERFIDAIKASGEDNNAKLVAAYFRHRGVEAHYVSPKDAGLFVTSEPGNAQVL
PESYDNLFQLRQQKGIIIFPGFFGFSQEGEVMTFSRSGSDITGSILANGLKAELYENFTDVDAVYSVNPAIVSNPKEIRE
LTYREMRELSYAGFSVFHDEALIPAFRARIPVQIKNTNNPDAEGTRVVATRDNTNGPVVGIAGDSGFCSIYISKYLMNRE
IGFGRRVLQILEDEGLTYEHIPSGIDDITIILRQNQMDQAAEKRMIKRIKEELDADEVAIEHNIALIMVVGEAMRHNVGT
TARASKALSEAGVNIEMINQGSSEVSMMFGVKEAQEKQAVRALYQEFFAKVLV
>Mature_453_residues
MKVVKFGGSSLASGAQLEKVFQIVTSDPERRAVVVSAPGKRHPEDTKVTDLLIECAQQYLLSKEATGLVEAIVGRYADIA
LELGLADDVINRIRADLLQLLEGDKTNPERFIDAIKASGEDNNAKLVAAYFRHRGVEAHYVSPKDAGLFVTSEPGNAQVL
PESYDNLFQLRQQKGIIIFPGFFGFSQEGEVMTFSRSGSDITGSILANGLKAELYENFTDVDAVYSVNPAIVSNPKEIRE
LTYREMRELSYAGFSVFHDEALIPAFRARIPVQIKNTNNPDAEGTRVVATRDNTNGPVVGIAGDSGFCSIYISKYLMNRE
IGFGRRVLQILEDEGLTYEHIPSGIDDITIILRQNQMDQAAEKRMIKRIKEELDADEVAIEHNIALIMVVGEAMRHNVGT
TARASKALSEAGVNIEMINQGSSEVSMMFGVKEAQEKQAVRALYQEFFAKVLV

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=474, Percent_Identity=27.6371308016878, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790455, Length=465, Percent_Identity=26.4516129032258, Blast_Score=106, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320893, Length=498, Percent_Identity=22.6907630522088, Blast_Score=109, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 49943; Mature: 49943

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVKFGGSSLASGAQLEKVFQIVTSDPERRAVVVSAPGKRHPEDTKVTDLLIECAQQYL
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHH
LSKEATGLVEAIVGRYADIALELGLADDVINRIRADLLQLLEGDKTNPERFIDAIKASGE
HHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
DNNAKLVAAYFRHRGVEAHYVSPKDAGLFVTSEPGNAQVLPESYDNLFQLRQQKGIIIFP
CCCCEEEEEHHHHCCCEEEEECCCCCEEEEECCCCCCEECCHHHHHHHHHHHHCCEEEEC
GFFGFSQEGEVMTFSRSGSDITGSILANGLKAELYENFTDVDAVYSVNPAIVSNPKEIRE
CCCCCCCCCCEEEEECCCCCCCHHHHHCCHHHHHHHCCCCCCHHEECCCHHHCCHHHHHH
LTYREMRELSYAGFSVFHDEALIPAFRARIPVQIKNTNNPDAEGTRVVATRDNTNGPVVG
HHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCEEE
IAGDSGFCSIYISKYLMNREIGFGRRVLQILEDEGLTYEHIPSGIDDITIILRQNQMDQA
EECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCCHHHH
AEKRMIKRIKEELDADEVAIEHNIALIMVVGEAMRHNVGTTARASKALSEAGVNIEMINQ
HHHHHHHHHHHHCCHHHEEEECCEEEEEEECHHHHCCCCCHHHHHHHHHHCCCEEEEECC
GSSEVSMMFGVKEAQEKQAVRALYQEFFAKVLV
CCCCEEHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVVKFGGSSLASGAQLEKVFQIVTSDPERRAVVVSAPGKRHPEDTKVTDLLIECAQQYL
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHH
LSKEATGLVEAIVGRYADIALELGLADDVINRIRADLLQLLEGDKTNPERFIDAIKASGE
HHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
DNNAKLVAAYFRHRGVEAHYVSPKDAGLFVTSEPGNAQVLPESYDNLFQLRQQKGIIIFP
CCCCEEEEEHHHHCCCEEEEECCCCCEEEEECCCCCCEECCHHHHHHHHHHHHCCEEEEC
GFFGFSQEGEVMTFSRSGSDITGSILANGLKAELYENFTDVDAVYSVNPAIVSNPKEIRE
CCCCCCCCCCEEEEECCCCCCCHHHHHCCHHHHHHHCCCCCCHHEECCCHHHCCHHHHHH
LTYREMRELSYAGFSVFHDEALIPAFRARIPVQIKNTNNPDAEGTRVVATRDNTNGPVVG
HHHHHHHHHHHCCHHHHCCCHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCCCCEEE
IAGDSGFCSIYISKYLMNREIGFGRRVLQILEDEGLTYEHIPSGIDDITIILRQNQMDQA
EECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCCHHHH
AEKRMIKRIKEELDADEVAIEHNIALIMVVGEAMRHNVGTTARASKALSEAGVNIEMINQ
HHHHHHHHHHHHCCHHHEEEECCEEEEEEECHHHHCCCCCHHHHHHHHHHCCCEEEEECC
GSSEVSMMFGVKEAQEKQAVRALYQEFFAKVLV
CCCCEEHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]