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The map label for this gene is nasA [H]

Identifier: 52784187

GI number: 52784187

Start: 340626

End: 341831

Strand: Reverse

Name: nasA [H]

Synonym: BLi00365

Alternate gene names: 52784187

Gene position: 341831-340626 (Counterclockwise)

Preceding gene: 52784191

Following gene: 52784185

Centisome position: 8.1

GC content: 51.91

Gene sequence:

>1206_bases
TTGAAATTGGCGGATTTGAGGAAGAGCGGACACGCGCCTTCCTTGTTGTCATCTTTTTTATATTTCGATGTCAGCTTTAT
GATTTGGGTGTTGCTGGGGGCGCTTGGTGTTTATGTTACGGCTGATTTTGATTTATCGCCTTCACAGGTCGGATTGATTG
TCGCCATACCTACTTTGGCCGGATCGTTTTTCCGGATTGCGCTCGGCATTTTAACCGACCGTTTCGGACCGAAAAAGACG
GCTGTTGGCGGAATGCTGGTGACGATGATTCCGTTGTTATGGGGATGGCAGTTCGGTTCATCGGTCGCCGAGCTGTATAT
CATCGGAATTTTACTCGGTGTGGCAGGCGCAAGCTTTTCCGCTTCCCTTCCTATGGCGAGCCGCTGGTATCCGCCGCACT
TGCAAGGTCTTGCCATGGGGATCGCCGGGGCGGGAAACAGCGGAACACTGCTTGCTACGCTATTCGGCCCTCGCCTTGCG
GAGCAAGTGGGCTGGAACGGCGTCATGGGACTGGCGCTGATTCCTTTAACCCTCGTGTTATTCAGCTATTTCCTTATGGC
TAAAGAACCTCCGAATCAGCCGGCTCCTAAACCGCTCAAAGACTATTTTGCCGTTTTCAAAGAAAGGGACACTTGGTATT
TTTGCCTTCTCTACAGCGTGACATTCGGGGGTTTTGTCGGCCTGTCCAACTTTTTAAGCTACTTTTTTGCATCCCAGTAT
GCCGTTCCGGCGGTAAGAGCCGGCGAATTTGTCACGCTTGTCGTTGCATCCGGAAGCTGCCTCCGCCCTGTCGGCGGATT
GATTGCCGATAAAATCGGCGGCGTGCGTTTGCTTCAATTCCTCTTTATCGGAGTGGCTCTGTGCATGTTCTCGGTCAGTA
TGCTCCCGTCTCTAGGCGTTGTCACAGCCGCATTATTTATCGGCATGGGCTGCCTCGGAATGGGGAACGGCGCGATATTC
CAGTTTGTACCGCAGCGATTTCAACAAGAGATCGGGATGGTGACGGGGATCGTCGGCGCTGCGGGGGGAATCGGCGGATT
CTTCCTTCCGAATATCCTCGGAACGCTGAAGCAAATTACAGGTACGTATTCAAGCGGGTTCCTAACCTTTACCGTTATTG
CATTTTTAGCGCTCGGTCTGCTTTTGTTTGCTCAGGCCGGATGGAAAAAACAGTGGAAAGCCGAGGAGAAATCAATCCGA
ATTTAA

Upstream 100 bases:

>100_bases
CAATCTTTTAGTTTGTTAGTAAAGCTTACAAAAATACTGCCAACCCTATTCGATTTTGTCATGATAAATTCTAGAATCTA
TCGGATGGGGAGTGTTGCAA

Downstream 100 bases:

>100_bases
GGGGTAGCCGCTCGATTCTTTTCGGGCGGTTTTCTATGCGGCATATCCTGTTTGATCTTTTGGTTATCCGGCCCGATCAT
GACAATTGGATGCTCATCAT

Product: NasA

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKLADLRKSGHAPSLLSSFLYFDVSFMIWVLLGALGVYVTADFDLSPSQVGLIVAIPTLAGSFFRIALGILTDRFGPKKT
AVGGMLVTMIPLLWGWQFGSSVAELYIIGILLGVAGASFSASLPMASRWYPPHLQGLAMGIAGAGNSGTLLATLFGPRLA
EQVGWNGVMGLALIPLTLVLFSYFLMAKEPPNQPAPKPLKDYFAVFKERDTWYFCLLYSVTFGGFVGLSNFLSYFFASQY
AVPAVRAGEFVTLVVASGSCLRPVGGLIADKIGGVRLLQFLFIGVALCMFSVSMLPSLGVVTAALFIGMGCLGMGNGAIF
QFVPQRFQQEIGMVTGIVGAAGGIGGFFLPNILGTLKQITGTYSSGFLTFTVIAFLALGLLLFAQAGWKKQWKAEEKSIR
I

Sequences:

>Translated_401_residues
MKLADLRKSGHAPSLLSSFLYFDVSFMIWVLLGALGVYVTADFDLSPSQVGLIVAIPTLAGSFFRIALGILTDRFGPKKT
AVGGMLVTMIPLLWGWQFGSSVAELYIIGILLGVAGASFSASLPMASRWYPPHLQGLAMGIAGAGNSGTLLATLFGPRLA
EQVGWNGVMGLALIPLTLVLFSYFLMAKEPPNQPAPKPLKDYFAVFKERDTWYFCLLYSVTFGGFVGLSNFLSYFFASQY
AVPAVRAGEFVTLVVASGSCLRPVGGLIADKIGGVRLLQFLFIGVALCMFSVSMLPSLGVVTAALFIGMGCLGMGNGAIF
QFVPQRFQQEIGMVTGIVGAAGGIGGFFLPNILGTLKQITGTYSSGFLTFTVIAFLALGLLLFAQAGWKKQWKAEEKSIR
I
>Mature_401_residues
MKLADLRKSGHAPSLLSSFLYFDVSFMIWVLLGALGVYVTADFDLSPSQVGLIVAIPTLAGSFFRIALGILTDRFGPKKT
AVGGMLVTMIPLLWGWQFGSSVAELYIIGILLGVAGASFSASLPMASRWYPPHLQGLAMGIAGAGNSGTLLATLFGPRLA
EQVGWNGVMGLALIPLTLVLFSYFLMAKEPPNQPAPKPLKDYFAVFKERDTWYFCLLYSVTFGGFVGLSNFLSYFFASQY
AVPAVRAGEFVTLVVASGSCLRPVGGLIADKIGGVRLLQFLFIGVALCMFSVSMLPSLGVVTAALFIGMGCLGMGNGAIF
QFVPQRFQQEIGMVTGIVGAAGGIGGFFLPNILGTLKQITGTYSSGFLTFTVIAFLALGLLLFAQAGWKKQWKAEEKSIR
I

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=396, Percent_Identity=27.2727272727273, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787475, Length=401, Percent_Identity=23.4413965087282, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42765; Mature: 42765

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLADLRKSGHAPSLLSSFLYFDVSFMIWVLLGALGVYVTADFDLSPSQVGLIVAIPTLA
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEEHHHH
GSFFRIALGILTDRFGPKKTAVGGMLVTMIPLLWGWQFGSSVAELYIIGILLGVAGASFS
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ASLPMASRWYPPHLQGLAMGIAGAGNSGTLLATLFGPRLAEQVGWNGVMGLALIPLTLVL
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
FSYFLMAKEPPNQPAPKPLKDYFAVFKERDTWYFCLLYSVTFGGFVGLSNFLSYFFASQY
HHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
AVPAVRAGEFVTLVVASGSCLRPVGGLIADKIGGVRLLQFLFIGVALCMFSVSMLPSLGV
CCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
VTAALFIGMGCLGMGNGAIFQFVPQRFQQEIGMVTGIVGAAGGIGGFFLPNILGTLKQIT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
GTYSSGFLTFTVIAFLALGLLLFAQAGWKKQWKAEEKSIRI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCC
>Mature Secondary Structure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CCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]