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The map label for this gene is cysS

Identifier: 52783950

GI number: 52783950

Start: 112207

End: 113610

Strand: Direct

Name: cysS

Synonym: BLi00112

Alternate gene names: 52783950

Gene position: 112207-113610 (Clockwise)

Preceding gene: 52783949

Following gene: 52783951

Centisome position: 2.66

GC content: 45.01

Gene sequence:

>1404_bases
GTGACACTAACATTGTATAATACTCTCACACGAAAAAAAGAGCCGTTTGTGCCTCTTGAAGAAGGCAAGGTCAAAATGTA
TGTGTGCGGTCCGACAGTATATAACTACATCCATATCGGAAACGCAAGGCCGGCTATCGTGTTTGACACTGTCAGAAAGT
ATTTAGAGTATAAAGGCTATGAAGTCCAATATGTCTCCAATTTTACAGATGTCGATGACAAATTAATTAAAGCGGCAAAT
GAACTTGGAGAGGAAGTGCCGGTCATCGCCGAACGCTTTATTAAGGCATACTTCGAAGATGTCGGTTCACTGGGCTGCAC
AAAAGCTGACTGCCATCCGCGCGTCACTGAAAACATGGATGAGATCATCGACTTTATTCAGACGCTGATCGATAAGGGCT
ATGCCTATGAGGCGGACGGAGATGTTTATTATAAAACAAGATCGTTTGAAGGCTATGGAAAGCTGTCGCATCAATCGGTC
GATGAGCTGAGATCAGGCGCCCGAATTCAAGTGGGCGAAAAGAAAGAAGATGCGCTCGATTTTACACTATGGAAAGCGGC
TAAAGATAACGAAATCTCCTGGGATAGCCCTTGGGGAAAAGGCCGTCCCGGCTGGCATATCGAGTGCTCTGCGATGGCGA
GAAAATATCTGGGCGATTCGATCGACATTCACGCCGGCGGTCAGGATCTGGCCTTCCCGCATCATGAAAACGAAATTGCC
CAATCGGAAGCATTAACAGGGAAGCCTTTCGCGAAATATTGGCTTCATAACGGCTACATTAATATCGATAATGAGAAAAT
GTCAAAATCGCTCGGCAACTTTGTTCTTGTGCATGACATTATTAAAGAGCATGACCCCCAAGTGCTGCGGTTTTTCATGC
TGTCTGTTCATTACAGACATCCGATAAACTATTCGGTCGAACTTCTCGAGAACACAAAAAACGCTTTTGGACGTCTAAAA
ACAGCTTATAGCAATTTGCAGCATCGCCTAAAAAGCAGTACAAATTTAACAGAGGGCGATTCAGAATGGCTTGAAAAAAT
CGAAGAGCAGCGAAAAGCCTTCCAAACGGCTATGGATGATGATTTCAATACAGCGAATGCCATTTCCGTCCTGTTTGACC
TGGCCAAACATGCCAACTACTATTTGCAGGAAAAGAATACTGCAGAACATGTCATTCAGGCGTTTATACAGCTGTTTGAC
GAGATCTGCTCAGTGCTCGGCTTCTCCCTCAAGGAACAGGAACTTTTGGATAAAGACATTGAAGAACTGATCGAGAAGCG
GAATGAGGCCCGGAGAAACCGCGACTTTGCAACATCTGATCAGATTCGCGACCAGTTGAAAAGCATGAATATTATCTTGG
AGGACACTCCGCAGGGCACGCGCTGGAAAAGAGGAGAATCATAA

Upstream 100 bases:

>100_bases
AGGATTTGCCGGATCCGGTGGCTGACCGCTTCAGAGAATTGGAAAACGAAATCAGGCAGCTGAAACAAGAGCTCCGGAGA
AAAGAAAGGGAAGATGAGCT

Downstream 100 bases:

>100_bases
ACATGAATTTGGAAACCATTAAAGACGCCAAACAGCTGAATGGTTTGGCGCTTGCTTACATGGGTGATGCCGTTTTGGAG
GTATATGTGAGGCATCACCT

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTLTLYNTLTRKKEPFVPLEEGKVKMYVCGPTVYNYIHIGNARPAIVFDTVRKYLEYKGYEVQYVSNFTDVDDKLIKAAN
ELGEEVPVIAERFIKAYFEDVGSLGCTKADCHPRVTENMDEIIDFIQTLIDKGYAYEADGDVYYKTRSFEGYGKLSHQSV
DELRSGARIQVGEKKEDALDFTLWKAAKDNEISWDSPWGKGRPGWHIECSAMARKYLGDSIDIHAGGQDLAFPHHENEIA
QSEALTGKPFAKYWLHNGYINIDNEKMSKSLGNFVLVHDIIKEHDPQVLRFFMLSVHYRHPINYSVELLENTKNAFGRLK
TAYSNLQHRLKSSTNLTEGDSEWLEKIEEQRKAFQTAMDDDFNTANAISVLFDLAKHANYYLQEKNTAEHVIQAFIQLFD
EICSVLGFSLKEQELLDKDIEELIEKRNEARRNRDFATSDQIRDQLKSMNIILEDTPQGTRWKRGES

Sequences:

>Translated_467_residues
MTLTLYNTLTRKKEPFVPLEEGKVKMYVCGPTVYNYIHIGNARPAIVFDTVRKYLEYKGYEVQYVSNFTDVDDKLIKAAN
ELGEEVPVIAERFIKAYFEDVGSLGCTKADCHPRVTENMDEIIDFIQTLIDKGYAYEADGDVYYKTRSFEGYGKLSHQSV
DELRSGARIQVGEKKEDALDFTLWKAAKDNEISWDSPWGKGRPGWHIECSAMARKYLGDSIDIHAGGQDLAFPHHENEIA
QSEALTGKPFAKYWLHNGYINIDNEKMSKSLGNFVLVHDIIKEHDPQVLRFFMLSVHYRHPINYSVELLENTKNAFGRLK
TAYSNLQHRLKSSTNLTEGDSEWLEKIEEQRKAFQTAMDDDFNTANAISVLFDLAKHANYYLQEKNTAEHVIQAFIQLFD
EICSVLGFSLKEQELLDKDIEELIEKRNEARRNRDFATSDQIRDQLKSMNIILEDTPQGTRWKRGES
>Mature_466_residues
TLTLYNTLTRKKEPFVPLEEGKVKMYVCGPTVYNYIHIGNARPAIVFDTVRKYLEYKGYEVQYVSNFTDVDDKLIKAANE
LGEEVPVIAERFIKAYFEDVGSLGCTKADCHPRVTENMDEIIDFIQTLIDKGYAYEADGDVYYKTRSFEGYGKLSHQSVD
ELRSGARIQVGEKKEDALDFTLWKAAKDNEISWDSPWGKGRPGWHIECSAMARKYLGDSIDIHAGGQDLAFPHHENEIAQ
SEALTGKPFAKYWLHNGYINIDNEKMSKSLGNFVLVHDIIKEHDPQVLRFFMLSVHYRHPINYSVELLENTKNAFGRLKT
AYSNLQHRLKSSTNLTEGDSEWLEKIEEQRKAFQTAMDDDFNTANAISVLFDLAKHANYYLQEKNTAEHVIQAFIQLFDE
ICSVLGFSLKEQELLDKDIEELIEKRNEARRNRDFATSDQIRDQLKSMNIILEDTPQGTRWKRGES

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=477, Percent_Identity=35.6394129979036, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI303304987, Length=313, Percent_Identity=35.7827476038339, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI62240992, Length=310, Percent_Identity=36.1290322580645, Blast_Score=202, Evalue=8e-52,
Organism=Homo sapiens, GI10835051, Length=310, Percent_Identity=36.1290322580645, Blast_Score=202, Evalue=8e-52,
Organism=Homo sapiens, GI21269877, Length=313, Percent_Identity=35.7827476038339, Blast_Score=201, Evalue=8e-52,
Organism=Escherichia coli, GI1786737, Length=474, Percent_Identity=44.5147679324894, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17509797, Length=337, Percent_Identity=32.9376854599407, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17509795, Length=337, Percent_Identity=32.9376854599407, Blast_Score=180, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324082, Length=367, Percent_Identity=35.9673024523161, Blast_Score=203, Evalue=6e-53,
Organism=Drosophila melanogaster, GI19922218, Length=501, Percent_Identity=31.936127744511, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI19922364, Length=317, Percent_Identity=35.6466876971609, Blast_Score=188, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_BACLD (Q65PC8)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077380.1
- RefSeq:   YP_089779.1
- ProteinModelPortal:   Q65PC8
- STRING:   Q65PC8
- EnsemblBacteria:   EBBACT00000054570
- EnsemblBacteria:   EBBACT00000059958
- GeneID:   3028867
- GeneID:   3099023
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00112
- KEGG:   bli:BL03269
- NMPDR:   fig|279010.5.peg.1489
- eggNOG:   COG0215
- GeneTree:   EBGT00050000000801
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   BLIC279010-1:BLI00112-MONOMER
- BioCyc:   BLIC279010:BL03269-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 53808; Mature: 53677

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 269-269

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTLYNTLTRKKEPFVPLEEGKVKMYVCGPTVYNYIHIGNARPAIVFDTVRKYLEYKGY
CEEEEEEHHHCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCC
EVQYVSNFTDVDDKLIKAANELGEEVPVIAERFIKAYFEDVGSLGCTKADCHPRVTENMD
EEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHH
EIIDFIQTLIDKGYAYEADGDVYYKTRSFEGYGKLSHQSVDELRSGARIQVGEKKEDALD
HHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHH
FTLWKAAKDNEISWDSPWGKGRPGWHIECSAMARKYLGDSIDIHAGGQDLAFPHHENEIA
HHHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHH
QSEALTGKPFAKYWLHNGYINIDNEKMSKSLGNFVLVHDIIKEHDPQVLRFFMLSVHYRH
HHHCCCCCHHHHHHHCCCEEEECHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
PINYSVELLENTKNAFGRLKTAYSNLQHRLKSSTNLTEGDSEWLEKIEEQRKAFQTAMDD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
DFNTANAISVLFDLAKHANYYLQEKNTAEHVIQAFIQLFDEICSVLGFSLKEQELLDKDI
CCCHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EELIEKRNEARRNRDFATSDQIRDQLKSMNIILEDTPQGTRWKRGES
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TLTLYNTLTRKKEPFVPLEEGKVKMYVCGPTVYNYIHIGNARPAIVFDTVRKYLEYKGY
EEEEEEHHHCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCC
EVQYVSNFTDVDDKLIKAANELGEEVPVIAERFIKAYFEDVGSLGCTKADCHPRVTENMD
EEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCHH
EIIDFIQTLIDKGYAYEADGDVYYKTRSFEGYGKLSHQSVDELRSGARIQVGEKKEDALD
HHHHHHHHHHHCCCEEECCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHH
FTLWKAAKDNEISWDSPWGKGRPGWHIECSAMARKYLGDSIDIHAGGQDLAFPHHENEIA
HHHHHCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHH
QSEALTGKPFAKYWLHNGYINIDNEKMSKSLGNFVLVHDIIKEHDPQVLRFFMLSVHYRH
HHHCCCCCHHHHHHHCCCEEEECHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
PINYSVELLENTKNAFGRLKTAYSNLQHRLKSSTNLTEGDSEWLEKIEEQRKAFQTAMDD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
DFNTANAISVLFDLAKHANYYLQEKNTAEHVIQAFIQLFDEICSVLGFSLKEQELLDKDI
CCCHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
EELIEKRNEARRNRDFATSDQIRDQLKSMNIILEDTPQGTRWKRGES
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA