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The map label for this gene is ksgA

Identifier: 52783899

GI number: 52783899

Start: 54918

End: 55796

Strand: Direct

Name: ksgA

Synonym: BLi00055

Alternate gene names: 52783899

Gene position: 54918-55796 (Clockwise)

Preceding gene: 52783898

Following gene: 52783900

Centisome position: 1.3

GC content: 47.67

Gene sequence:

>879_bases
ATGAATAAAGATATCGCCACCCCAATTAGGACTAAGGAAATTCTCAATAAATACGGATTTTCATTCAAAAAAAGCCTGGG
GCAGAACTTCTTGATTGACACGAACATATTGGACAGAATTGTCGACCATGCCGGTGTGACGGAGCGTACAGGCGTGATTG
AAATCGGTCCGGGGATCGGGGCATTGACCGAGCAGCTGGCAAAGCGTGCGAAGAAAGTAACGGCCTTTGAGATCGACCAA
CGGCTTCTGCCGATTTTAGAAGATACGCTTTCTCCGTATGATAATGTGACTGTCATTCACCAGGATGTCCTGAAGGCCGA
TGTCCGAGCGGTAATGGACGAACAGTTTCAAGACTGCGATGAGGTGATGGTTGTCGCCAACCTCCCGTATTACGTCACGA
CTCCGATCATCATGAAGCTTTTAGAAGAAAATCTGCCGCTGAAAGGAATCGTCGTCATGCTTCAAAAAGAGGTGGCGGAC
CGAATGGCTGCAAAACCTTCTTCAAAGGAATACGGGTCCCTTTCCATCGCGGTGCAGTTTTATACGGAGGCCAAAACGGT
CATGAATGTACCAAAGACCGTATTTGTCCCTCAGCCTAACGTAGATTCCGCGGTCATCAGGCTGACGCTGAGGAAAGAGC
CGGCTGTGGCCGTTCAAGATGCTGCCTTTTTCTTTCAAGTCGTAAAAGCAAGCTTTGCACAGCGTCGAAAAACGCTTTTC
AACAACCTCGTCAACAATCTGCCGAATGGCAAAGAGAATAAATCAAAAATTGAAAGAGCGCTTCAGGATTCACATATCGA
CGGAAAACGACGCGGAGAGTCGCTTAGCATTGAAGAGTTCGCCGTCTTATCTGACCGCTTGAGAGAAGTCCTTCTTTAA

Upstream 100 bases:

>100_bases
TATACAAATGGGAAACAGCTTCAAAAGCGGCTGCAAATGTTTCAGATCAAAAAGAGTGATTATTTAAGCGCACTGGATGC
TGTGCTGCGGGAGGAAGAAC

Downstream 100 bases:

>100_bases
TGGAGGGCTTTTTTTATTGATGCCGGGCTTTAAGCCTGATCAGGCTTGTCATCCGTTCACCACTTGAAGGGCAGGCACAT
AGGCTAAAGAAGCACCTTTA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MNKDIATPIRTKEILNKYGFSFKKSLGQNFLIDTNILDRIVDHAGVTERTGVIEIGPGIGALTEQLAKRAKKVTAFEIDQ
RLLPILEDTLSPYDNVTVIHQDVLKADVRAVMDEQFQDCDEVMVVANLPYYVTTPIIMKLLEENLPLKGIVVMLQKEVAD
RMAAKPSSKEYGSLSIAVQFYTEAKTVMNVPKTVFVPQPNVDSAVIRLTLRKEPAVAVQDAAFFFQVVKASFAQRRKTLF
NNLVNNLPNGKENKSKIERALQDSHIDGKRRGESLSIEEFAVLSDRLREVLL

Sequences:

>Translated_292_residues
MNKDIATPIRTKEILNKYGFSFKKSLGQNFLIDTNILDRIVDHAGVTERTGVIEIGPGIGALTEQLAKRAKKVTAFEIDQ
RLLPILEDTLSPYDNVTVIHQDVLKADVRAVMDEQFQDCDEVMVVANLPYYVTTPIIMKLLEENLPLKGIVVMLQKEVAD
RMAAKPSSKEYGSLSIAVQFYTEAKTVMNVPKTVFVPQPNVDSAVIRLTLRKEPAVAVQDAAFFFQVVKASFAQRRKTLF
NNLVNNLPNGKENKSKIERALQDSHIDGKRRGESLSIEEFAVLSDRLREVLL
>Mature_292_residues
MNKDIATPIRTKEILNKYGFSFKKSLGQNFLIDTNILDRIVDHAGVTERTGVIEIGPGIGALTEQLAKRAKKVTAFEIDQ
RLLPILEDTLSPYDNVTVIHQDVLKADVRAVMDEQFQDCDEVMVVANLPYYVTTPIIMKLLEENLPLKGIVVMLQKEVAD
RMAAKPSSKEYGSLSIAVQFYTEAKTVMNVPKTVFVPQPNVDSAVIRLTLRKEPAVAVQDAAFFFQVVKASFAQRRKTLF
NNLVNNLPNGKENKSKIERALQDSHIDGKRRGESLSIEEFAVLSDRLREVLL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=296, Percent_Identity=31.0810810810811, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI7657198, Length=225, Percent_Identity=34.2222222222222, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1786236, Length=271, Percent_Identity=32.4723247232472, Blast_Score=144, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI25141369, Length=300, Percent_Identity=29.6666666666667, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25146882, Length=228, Percent_Identity=32.8947368421053, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324989, Length=224, Percent_Identity=29.4642857142857, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21358017, Length=271, Percent_Identity=32.1033210332103, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21357273, Length=289, Percent_Identity=26.643598615917, Blast_Score=104, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_BACLD (Q65PH9)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_077328.1
- RefSeq:   YP_089728.1
- ProteinModelPortal:   Q65PH9
- SMR:   Q65PH9
- STRING:   Q65PH9
- EnsemblBacteria:   EBBACT00000054262
- EnsemblBacteria:   EBBACT00000060771
- GeneID:   3028653
- GeneID:   3100522
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi00055
- KEGG:   bli:BL00529
- NMPDR:   fig|279010.5.peg.1337
- eggNOG:   COG0030
- GeneTree:   EBGT00050000002781
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- BioCyc:   BLIC279010-1:BLI00055-MONOMER
- BioCyc:   BLIC279010:BL00529-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 32759; Mature: 32759

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 29-29 BINDING 31-31 BINDING 56-56 BINDING 77-77 BINDING 102-102 BINDING 127-127

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDIATPIRTKEILNKYGFSFKKSLGQNFLIDTNILDRIVDHAGVTERTGVIEIGPGIG
CCCCCCCCHHHHHHHHHHCCHHHHHCCCCEEECHHHHHHHHHHCCCCCCCCEEEECCCCH
ALTEQLAKRAKKVTAFEIDQRLLPILEDTLSPYDNVTVIHQDVLKADVRAVMDEQFQDCD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EVMVVANLPYYVTTPIIMKLLEENLPLKGIVVMLQKEVADRMAAKPSSKEYGSLSIAVQF
HEEHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
YTEAKTVMNVPKTVFVPQPNVDSAVIRLTLRKEPAVAVQDAAFFFQVVKASFAQRRKTLF
HHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NNLVNNLPNGKENKSKIERALQDSHIDGKRRGESLSIEEFAVLSDRLREVLL
HHHHHHCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKDIATPIRTKEILNKYGFSFKKSLGQNFLIDTNILDRIVDHAGVTERTGVIEIGPGIG
CCCCCCCCHHHHHHHHHHCCHHHHHCCCCEEECHHHHHHHHHHCCCCCCCCEEEECCCCH
ALTEQLAKRAKKVTAFEIDQRLLPILEDTLSPYDNVTVIHQDVLKADVRAVMDEQFQDCD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EVMVVANLPYYVTTPIIMKLLEENLPLKGIVVMLQKEVADRMAAKPSSKEYGSLSIAVQF
HEEHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
YTEAKTVMNVPKTVFVPQPNVDSAVIRLTLRKEPAVAVQDAAFFFQVVKASFAQRRKTLF
HHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NNLVNNLPNGKENKSKIERALQDSHIDGKRRGESLSIEEFAVLSDRLREVLL
HHHHHHCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA