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The map label for this gene is holB [H]

Identifier: 52783888

GI number: 52783888

Start: 44934

End: 45923

Strand: Direct

Name: holB [H]

Synonym: BLi00044

Alternate gene names: 52783888

Gene position: 44934-45923 (Clockwise)

Preceding gene: 52783887

Following gene: 52783889

Centisome position: 1.06

GC content: 43.23

Gene sequence:

>990_bases
ATGGCTACAACCTGGACAGAAATGCAGAAGCTTCAGCCACGGGTTATGAAGCTTTTGCAAAATAGTATAGAAAGAAATCG
GCTTGCCCACGCCTATTTATTTGAAGGGCGCAAAGGGACGGGCAAATTAGACGCGGCAACCCTTCTGGCAAAAAGCTTTT
TTTGCCTTGAAGATGGAGCAGAGCCATGTGAGTCATGCAGGAACTGCCAGCGGATTGAATCTGGAAACCATCCGGACGTA
CACGTCGTCCATCCGGACGGCCTTTCCATTAAAAAGGGACAAATCCAGGCGCTTCAGGAAGAGTTTTCTAAGACCGGTTT
GGAATCGCATAAAAAGCTGTATATTATTTCCCATGCGGATCAAATGACGGTCAATGCGGCGAACAGCCTTCTAAAGTTTT
TAGAGGAACCGAGCAGCGATACAATCGCTGTTCTTTTAACGGAACAGCCGCAAAAATTGCTCGATACGATCATATCGAGA
TGCCAGGTTTTATCCTTTCAGTCGCTGCAGCCCGAATCGATTGAAAAGGAACTGGTCAAACAAGAGGTCTCACCGAATAT
TGCAGCCCTTTTGTCGAATATGACCAACAATTTATCGGAAGCGCTCGAATTGAGTCGAAATGATCAGTTTGCAGAGTCGC
GAGCGAAAGTGATAAAATTGTATGAAGTCTTACACCAGCGAAAAGGACATGCTTTTTTCTATATTCAAGATCAGTGGATG
CCCTTTTTCAAGGAGAAAGACCACCAAGAAATGGGTCTTGATATGCTTTTATTTATATATCGCGACGTGTTGTCCATTCA
GTTGGGACATGAAGATAAAATACTATTTCAGGACTTATTCCAATCATTAAAACAGCATGCGCTGCAAACAACACAACAAA
GAGTGACAAACCAGATACTTGCTGTTTTAGAAGCAAAAAAACGGCTTTATTCCAATGTGAATGCTCAAGGATTAATGGAG
CACTTGGTTTTAATGCTGCAGGAGGGATAA

Upstream 100 bases:

>100_bases
AGGATCTTATGGATCAAGAAAAATCCTCTATCGAACTTTTGGAACGCATTGGCGAAATAAAGGGTTTACTGATAAACCTT
TACACATAGAGAGTGATGGT

Downstream 100 bases:

>100_bases
GCTTGTACAACGTTATTGGTGTCCGCTTTAAAAAAGCGGGCAAAATATATTATTTCGATCCGAACGGATTTCAGATAGAT
AAAGACAGCTGTGTGATCGT

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MATTWTEMQKLQPRVMKLLQNSIERNRLAHAYLFEGRKGTGKLDAATLLAKSFFCLEDGAEPCESCRNCQRIESGNHPDV
HVVHPDGLSIKKGQIQALQEEFSKTGLESHKKLYIISHADQMTVNAANSLLKFLEEPSSDTIAVLLTEQPQKLLDTIISR
CQVLSFQSLQPESIEKELVKQEVSPNIAALLSNMTNNLSEALELSRNDQFAESRAKVIKLYEVLHQRKGHAFFYIQDQWM
PFFKEKDHQEMGLDMLLFIYRDVLSIQLGHEDKILFQDLFQSLKQHALQTTQQRVTNQILAVLEAKKRLYSNVNAQGLME
HLVLMLQEG

Sequences:

>Translated_329_residues
MATTWTEMQKLQPRVMKLLQNSIERNRLAHAYLFEGRKGTGKLDAATLLAKSFFCLEDGAEPCESCRNCQRIESGNHPDV
HVVHPDGLSIKKGQIQALQEEFSKTGLESHKKLYIISHADQMTVNAANSLLKFLEEPSSDTIAVLLTEQPQKLLDTIISR
CQVLSFQSLQPESIEKELVKQEVSPNIAALLSNMTNNLSEALELSRNDQFAESRAKVIKLYEVLHQRKGHAFFYIQDQWM
PFFKEKDHQEMGLDMLLFIYRDVLSIQLGHEDKILFQDLFQSLKQHALQTTQQRVTNQILAVLEAKKRLYSNVNAQGLME
HLVLMLQEG
>Mature_328_residues
ATTWTEMQKLQPRVMKLLQNSIERNRLAHAYLFEGRKGTGKLDAATLLAKSFFCLEDGAEPCESCRNCQRIESGNHPDVH
VVHPDGLSIKKGQIQALQEEFSKTGLESHKKLYIISHADQMTVNAANSLLKFLEEPSSDTIAVLLTEQPQKLLDTIISRC
QVLSFQSLQPESIEKELVKQEVSPNIAALLSNMTNNLSEALELSRNDQFAESRAKVIKLYEVLHQRKGHAFFYIQDQWMP
FFKEKDHQEMGLDMLLFIYRDVLSIQLGHEDKILFQDLFQSLKQHALQTTQQRVTNQILAVLEAKKRLYSNVNAQGLMEH
LVLMLQEG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=170, Percent_Identity=36.4705882352941, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787341, Length=177, Percent_Identity=30.5084745762712, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37606; Mature: 37475

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTWTEMQKLQPRVMKLLQNSIERNRLAHAYLFEGRKGTGKLDAATLLAKSFFCLEDGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
EPCESCRNCQRIESGNHPDVHVVHPDGLSIKKGQIQALQEEFSKTGLESHKKLYIISHAD
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCC
QMTVNAANSLLKFLEEPSSDTIAVLLTEQPQKLLDTIISRCQVLSFQSLQPESIEKELVK
HHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
QEVSPNIAALLSNMTNNLSEALELSRNDQFAESRAKVIKLYEVLHQRKGHAFFYIQDQWM
HHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
PFFKEKDHQEMGLDMLLFIYRDVLSIQLGHEDKILFQDLFQSLKQHALQTTQQRVTNQIL
CHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLEAKKRLYSNVNAQGLMEHLVLMLQEG
HHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ATTWTEMQKLQPRVMKLLQNSIERNRLAHAYLFEGRKGTGKLDAATLLAKSFFCLEDGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCC
EPCESCRNCQRIESGNHPDVHVVHPDGLSIKKGQIQALQEEFSKTGLESHKKLYIISHAD
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCC
QMTVNAANSLLKFLEEPSSDTIAVLLTEQPQKLLDTIISRCQVLSFQSLQPESIEKELVK
HHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
QEVSPNIAALLSNMTNNLSEALELSRNDQFAESRAKVIKLYEVLHQRKGHAFFYIQDQWM
HHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCC
PFFKEKDHQEMGLDMLLFIYRDVLSIQLGHEDKILFQDLFQSLKQHALQTTQQRVTNQIL
CHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLEAKKRLYSNVNAQGLMEHLVLMLQEG
HHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]