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The map label for this gene is pbpB [H]

Identifier: 52142849

GI number: 52142849

Start: 2529235

End: 2531373

Strand: Direct

Name: pbpB [H]

Synonym: BCZK2392

Alternate gene names: 52142849

Gene position: 2529235-2531373 (Clockwise)

Preceding gene: 52142848

Following gene: 52142847

Centisome position: 47.71

GC content: 34.5

Gene sequence:

>2139_bases
ATGCATAAACTTCAAACGAATAAAGGCGCGCGCTACTTTATGATTGCTTTTATTGTTTTGTTTCTTATTATGCTTTTGCG
TTTTTTCTATATTCAAGCAGTAGGGGTCGTGCATAATGTGAATGTAAAGGATCTTGCAACTGAACAACATAATAAAAATG
GTATTTTGGAAGCAAATCGTGGCACAATATATGATCAAGCTGGAAAAGTGTTAGTTCAAGATTCCACAACGTATCGTGTT
GTTGTGAATTTGAAAGGTAATGGCAAATTGAAAAATAAAGATGAAACCGCAGCGCAATTAGCTGATGCGCTTGAAATTAA
TAAAGAAGATGTGTTAAAGAATTTCCATGAAGGGCGTACGCAAGTTGAAATCGGAAAAGTTGGTCGAAATTTAACGAGAG
AAAAGAAAGAACAAATTAGAAAATTAAAAATCCCGGGTGTATCTTTTATGCCTGAGAAAGCAAGGGTGTATCCGAATGAA
GATTTTGCATCTTATATACTTGGTTTTGCTAGACCAGATGATAAAGGAAATACAGAAGGGAAATTTGGACTTGAAAAAAG
TTTGGATAAATATTTGCGCTCGACAAACGGGAATATAGAGTATGTGGGAGGGCGAGGAGGTATCCCGCTTACAAATGATA
TAGGAAAAATAGAACCTGCTAAACATGGAAATAACGTATATCTTACGCTTGATAAACAAATTAATAGTTTTTTAGAAGAA
GCGATGAATAAGGCGCAAGATCACTATGATCCTTCTATGTTATTAGGTATCATAGCGGATCCAAAGACGGGGAAAGTGTT
AGCTATGTCTAGTAAACCTAGTTTTAATCCTAATAAAGGGGATATTGAATACTTTTTAAATGATCCAATCGCCAATGCAT
ATGAACCAGGTTCAACGATGAAAGTATTTACGTTAGCCGCAGCAATTAATGAAGGGGTATATAACGGAAAAGAATATTTC
CAATCTGGAAAATATGCAGTTGGTTCATCTGATATAAAAGATCATAATGGTGGATTTGGATGGGGATCTATTACATTTGA
TGAAGGTTTTGAAAGATCATCAAACGTAGCTTTTTCTATTTTAGAAGATCAGTTATTAAAACCAAATAAATTTAAGCAAT
ATATGAACAAATTTGGTTTCAATCAAAAAACTGGAATAGATTTACCTGGTGAAAGTAAAAATACTTTACTATTAAATACA
CAAATTCAACAAGTAACAACTTCTTTCGGACAAGGTTCTACAGTAACTCCAATTCAATTAATTCAAGCCGCTACTGCTAT
AGCAAATGACGGGAAAATGATGCAACCCTATATCGTTGACAAAGTTGTAAATCCAACAAACAAAGAAGTTATTATGGAAA
ATCAATCGAAAGAAATTGGGAATCCGATAAAAAAAGAGACAGCGGACAAGGTAAGAGACTTAATGGAACGTGTTATTACG
TCTTCAAAAGGTACTGGTACAATGTATAAAGTGGATGGTTACCCAATAGGAGGTAAGACAGGAACAGCTCAAATTCCGAA
TCCTGAAAATGGAAGATACATGGAAGGAAAAGAAAATTATATTTTTTCATTTTTAGGTATGGCTCCGATTGATGACCCGG
AGTTAATTGTATACTTGGCAATTAAACAACCAAAGTTAGAGGGAGACGAGTATGGAGCTCAGCCGCTTGCTGAAATATTT
AAACCAGTTGTGAAAAATAGCCTTGAATATTTAAAGGTGAAACCATACACAGAAAAACAAATGGCCGATGCAACGGAAAA
ATCCCAATTAAAAGTGCAAAACTATGTGAATCAATCCATGGATACGGTAGAGAATAATGTAAAAAAAGAGAGGCTCCAAC
CAATAACTTTAGGAGAAGGAAAAATAATAAAACAATATCCGCAGTCTGGCGAAGTAATGAGTGAAGGAGATCGAATATTT
TTATTAGGAAAAAACGCTACGATGCCCAATTTACAAGGTTGGTCCATGCGTGATGTGATGTATTTTTCTAAACTATTAAA
ATTAGATTTAAAAACTTCAGGTACGGGATATGTAACAAGTCAAAGTATCGAAAAAGGACAAGCTATACAAGAAGACGATG
TATTAGAACTAGAATTAGAGCCACCGTTACAACCGTTAACAGAGGCAAATACAAATTAA

Upstream 100 bases:

>100_bases
ATTCATACGAAAACTTCGAAACATATAGTATTGAAAGTAAAAATTACATATTTTAAAGCGCATCTTTGCATGTATCAATT
AAAAAAGAAAGGAGCGAAAT

Downstream 100 bases:

>100_bases
TTAGGATATGTGAAGGGTCTATATATAATAGACTCTTTTTTTATGGTTCAAGTAATTTCACGCACCGTAATTTTTAGAAA
AAATAATAAATAAGATTTTT

Product: penicillin-binding protein transpeptidase domain-containing protein

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 712; Mature: 712

Protein sequence:

>712_residues
MHKLQTNKGARYFMIAFIVLFLIMLLRFFYIQAVGVVHNVNVKDLATEQHNKNGILEANRGTIYDQAGKVLVQDSTTYRV
VVNLKGNGKLKNKDETAAQLADALEINKEDVLKNFHEGRTQVEIGKVGRNLTREKKEQIRKLKIPGVSFMPEKARVYPNE
DFASYILGFARPDDKGNTEGKFGLEKSLDKYLRSTNGNIEYVGGRGGIPLTNDIGKIEPAKHGNNVYLTLDKQINSFLEE
AMNKAQDHYDPSMLLGIIADPKTGKVLAMSSKPSFNPNKGDIEYFLNDPIANAYEPGSTMKVFTLAAAINEGVYNGKEYF
QSGKYAVGSSDIKDHNGGFGWGSITFDEGFERSSNVAFSILEDQLLKPNKFKQYMNKFGFNQKTGIDLPGESKNTLLLNT
QIQQVTTSFGQGSTVTPIQLIQAATAIANDGKMMQPYIVDKVVNPTNKEVIMENQSKEIGNPIKKETADKVRDLMERVIT
SSKGTGTMYKVDGYPIGGKTGTAQIPNPENGRYMEGKENYIFSFLGMAPIDDPELIVYLAIKQPKLEGDEYGAQPLAEIF
KPVVKNSLEYLKVKPYTEKQMADATEKSQLKVQNYVNQSMDTVENNVKKERLQPITLGEGKIIKQYPQSGEVMSEGDRIF
LLGKNATMPNLQGWSMRDVMYFSKLLKLDLKTSGTGYVTSQSIEKGQAIQEDDVLELELEPPLQPLTEANTN

Sequences:

>Translated_712_residues
MHKLQTNKGARYFMIAFIVLFLIMLLRFFYIQAVGVVHNVNVKDLATEQHNKNGILEANRGTIYDQAGKVLVQDSTTYRV
VVNLKGNGKLKNKDETAAQLADALEINKEDVLKNFHEGRTQVEIGKVGRNLTREKKEQIRKLKIPGVSFMPEKARVYPNE
DFASYILGFARPDDKGNTEGKFGLEKSLDKYLRSTNGNIEYVGGRGGIPLTNDIGKIEPAKHGNNVYLTLDKQINSFLEE
AMNKAQDHYDPSMLLGIIADPKTGKVLAMSSKPSFNPNKGDIEYFLNDPIANAYEPGSTMKVFTLAAAINEGVYNGKEYF
QSGKYAVGSSDIKDHNGGFGWGSITFDEGFERSSNVAFSILEDQLLKPNKFKQYMNKFGFNQKTGIDLPGESKNTLLLNT
QIQQVTTSFGQGSTVTPIQLIQAATAIANDGKMMQPYIVDKVVNPTNKEVIMENQSKEIGNPIKKETADKVRDLMERVIT
SSKGTGTMYKVDGYPIGGKTGTAQIPNPENGRYMEGKENYIFSFLGMAPIDDPELIVYLAIKQPKLEGDEYGAQPLAEIF
KPVVKNSLEYLKVKPYTEKQMADATEKSQLKVQNYVNQSMDTVENNVKKERLQPITLGEGKIIKQYPQSGEVMSEGDRIF
LLGKNATMPNLQGWSMRDVMYFSKLLKLDLKTSGTGYVTSQSIEKGQAIQEDDVLELELEPPLQPLTEANTN
>Mature_712_residues
MHKLQTNKGARYFMIAFIVLFLIMLLRFFYIQAVGVVHNVNVKDLATEQHNKNGILEANRGTIYDQAGKVLVQDSTTYRV
VVNLKGNGKLKNKDETAAQLADALEINKEDVLKNFHEGRTQVEIGKVGRNLTREKKEQIRKLKIPGVSFMPEKARVYPNE
DFASYILGFARPDDKGNTEGKFGLEKSLDKYLRSTNGNIEYVGGRGGIPLTNDIGKIEPAKHGNNVYLTLDKQINSFLEE
AMNKAQDHYDPSMLLGIIADPKTGKVLAMSSKPSFNPNKGDIEYFLNDPIANAYEPGSTMKVFTLAAAINEGVYNGKEYF
QSGKYAVGSSDIKDHNGGFGWGSITFDEGFERSSNVAFSILEDQLLKPNKFKQYMNKFGFNQKTGIDLPGESKNTLLLNT
QIQQVTTSFGQGSTVTPIQLIQAATAIANDGKMMQPYIVDKVVNPTNKEVIMENQSKEIGNPIKKETADKVRDLMERVIT
SSKGTGTMYKVDGYPIGGKTGTAQIPNPENGRYMEGKENYIFSFLGMAPIDDPELIVYLAIKQPKLEGDEYGAQPLAEIF
KPVVKNSLEYLKVKPYTEKQMADATEKSQLKVQNYVNQSMDTVENNVKKERLQPITLGEGKIIKQYPQSGEVMSEGDRIF
LLGKNATMPNLQGWSMRDVMYFSKLLKLDLKTSGTGYVTSQSIEKGQAIQEDDVLELELEPPLQPLTEANTN

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=577, Percent_Identity=26.6897746967071, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1786854, Length=560, Percent_Identity=24.1071428571429, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 79312; Mature: 79312

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKLQTNKGARYFMIAFIVLFLIMLLRFFYIQAVGVVHNVNVKDLATEQHNKNGILEANR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCC
GTIYDQAGKVLVQDSTTYRVVVNLKGNGKLKNKDETAAQLADALEINKEDVLKNFHEGRT
CEEECCCCCEEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCC
QVEIGKVGRNLTREKKEQIRKLKIPGVSFMPEKARVYPNEDFASYILGFARPDDKGNTEG
EEEHHHHCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
KFGLEKSLDKYLRSTNGNIEYVGGRGGIPLTNDIGKIEPAKHGNNVYLTLDKQINSFLEE
CCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHH
AMNKAQDHYDPSMLLGIIADPKTGKVLAMSSKPSFNPNKGDIEYFLNDPIANAYEPGSTM
HHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCE
KVFTLAAAINEGVYNGKEYFQSGKYAVGSSDIKDHNGGFGWGSITFDEGFERSSNVAFSI
EHHHHHHHHHCCCCCCHHHHHCCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEE
LEDQLLKPNKFKQYMNKFGFNQKTGIDLPGESKNTLLLNTQIQQVTTSFGQGSTVTPIQL
HHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCCCCHHHH
IQAATAIANDGKMMQPYIVDKVVNPTNKEVIMENQSKEIGNPIKKETADKVRDLMERVIT
HHHHHHHCCCCCEECCHHHHHHCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHH
SSKGTGTMYKVDGYPIGGKTGTAQIPNPENGRYMEGKENYIFSFLGMAPIDDPELIVYLA
CCCCCCEEEEECCEECCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCCCEEEEEE
IKQPKLEGDEYGAQPLAEIFKPVVKNSLEYLKVKPYTEKQMADATEKSQLKVQNYVNQSM
EECCCCCCCCCCCHHHHHHHHHHHHCCHHEEEECCCCHHHHHCCHHHHHHHHHHHHHHHH
DTVENNVKKERLQPITLGEGKIIKQYPQSGEVMSEGDRIFLLGKNATMPNLQGWSMRDVM
HHHHHHHHHHHCCCEECCCCCHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHHH
YFSKLLKLDLKTSGTGYVTSQSIEKGQAIQEDDVLELELEPPLQPLTEANTN
HHHHHHHHCCCCCCCCEEECCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MHKLQTNKGARYFMIAFIVLFLIMLLRFFYIQAVGVVHNVNVKDLATEQHNKNGILEANR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCC
GTIYDQAGKVLVQDSTTYRVVVNLKGNGKLKNKDETAAQLADALEINKEDVLKNFHEGRT
CEEECCCCCEEEECCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCC
QVEIGKVGRNLTREKKEQIRKLKIPGVSFMPEKARVYPNEDFASYILGFARPDDKGNTEG
EEEHHHHCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
KFGLEKSLDKYLRSTNGNIEYVGGRGGIPLTNDIGKIEPAKHGNNVYLTLDKQINSFLEE
CCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHH
AMNKAQDHYDPSMLLGIIADPKTGKVLAMSSKPSFNPNKGDIEYFLNDPIANAYEPGSTM
HHHHHHHCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCE
KVFTLAAAINEGVYNGKEYFQSGKYAVGSSDIKDHNGGFGWGSITFDEGFERSSNVAFSI
EHHHHHHHHHCCCCCCHHHHHCCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEE
LEDQLLKPNKFKQYMNKFGFNQKTGIDLPGESKNTLLLNTQIQQVTTSFGQGSTVTPIQL
HHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCCCCHHHH
IQAATAIANDGKMMQPYIVDKVVNPTNKEVIMENQSKEIGNPIKKETADKVRDLMERVIT
HHHHHHHCCCCCEECCHHHHHHCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHH
SSKGTGTMYKVDGYPIGGKTGTAQIPNPENGRYMEGKENYIFSFLGMAPIDDPELIVYLA
CCCCCCEEEEECCEECCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCCCEEEEEE
IKQPKLEGDEYGAQPLAEIFKPVVKNSLEYLKVKPYTEKQMADATEKSQLKVQNYVNQSM
EECCCCCCCCCCCHHHHHHHHHHHHCCHHEEEECCCCHHHHHCCHHHHHHHHHHHHHHHH
DTVENNVKKERLQPITLGEGKIIKQYPQSGEVMSEGDRIFLLGKNATMPNLQGWSMRDVM
HHHHHHHHHHHCCCEECCCCCHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCHHHHH
YFSKLLKLDLKTSGTGYVTSQSIEKGQAIQEDDVLELELEPPLQPLTEANTN
HHHHHHHHCCCCCCCCEEECCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]