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The map label for this gene is pbpE [H]

Identifier: 52142846

GI number: 52142846

Start: 2533362

End: 2534384

Strand: Direct

Name: pbpE [H]

Synonym: BCZK2394

Alternate gene names: 52142846

Gene position: 2533362-2534384 (Clockwise)

Preceding gene: 52142847

Following gene: 52142845

Centisome position: 47.79

GC content: 32.55

Gene sequence:

>1023_bases
ATGAAAACAAAAGATCAAATAGATAACATTGTAAAAGAAACATATCGTCATATAGATTTCTCTGGGGTAATTTTAGTGAA
AGAAAAAAAGGGCATAGTTTATGAAGAAGCGTTTGGTTATGCAAATCGAAATGAGTGTATAAACAATACGCTACAAACTA
GGTTTGGAATTGCCTCTGGGTGTAAAATATTCACAGCTATCGGTGTCTGTCAACTTGTTGAAAATGGGGTAATTACTTTT
CAAACAAAGTTAAAAGAGTGTTTAAGTGTTAACTTTCCAAACTTTAATGAAGATATTACAATACATCACCTTTTGACGCA
TAGTTCGGGTATTCCAGATTATTTTGATGAAAGTATTATGGATAACTTCGAAGATCTTTGGAAACAAACACCTATGTATT
TATTAAAAAGTTTAAAAGACTTTTTACCACTATTTCAAAACAGAGATATGATGTATGCACCAGGAAGTAAGTTTCACTAC
AACAATGCAGGTTTTATCATACTGGGATTAATAATCGAAGAACAGACAGGACTTAAATTTACGGAGTATATAGAAAAAAA
CATATTTAATCCAATTGGTATGAATCATTCGGGCTATTTTTCTTTAAATAGATTACCAAGGCAGACAGCTCTCGGATATA
TAAAAGATGAAATTAGCCAGACATGGAGAACAAATGCTTACTCTATTCCGATAAAAGGTGGTTCGGATGGTGGAGCTTTT
ATTACTGCGTCAGACATGCTGAAGTTTTGGGAAGCGTTATTTAACTATGAAATTATAAGCCAAGAATATACAAAAATACT
TTTAACTCCTCACATCCAAGTAAATAATAATCAATCTTATGGCTATGGAATATGGATTGAAACAAGAGAAAACAAGGTAT
TCAAATATCATGTAATGGGATATGACCCAGGTGTCAGTTTTCGCTCGGCCGTATATCCAGACTTAGGAATTACATTAGTT
ATTCCGTCAAATAAAGGAGCAGGACCCGAAAAATTAATGATAGAAATAGAAGGAGCTTTTTAA

Upstream 100 bases:

>100_bases
TTGATTTAAATCTTACGATGTTCAATAGGAGGGTTATCCCTCCTATATATGGAAATATAAGAAAGGAGAACAATATTGAT
AAACGAGGGTGATTTTTGTA

Downstream 100 bases:

>100_bases
TTATATGGATATAGAAATTAGAGCGTATAGAAAAGAGGATGAAACCGGTTGGGTACGTTGCCGTGTGTTATCTTTTTTAG
ATACAGCTTATTACGATAAT

Product: penicillin-binding protein

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTLQTRFGIASGCKIFTAIGVCQLVENGVITF
QTKLKECLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYAPGSKFHY
NNAGFIILGLIIEEQTGLKFTEYIEKNIFNPIGMNHSGYFSLNRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSDGGAF
ITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKVFKYHVMGYDPGVSFRSAVYPDLGITLV
IPSNKGAGPEKLMIEIEGAF

Sequences:

>Translated_340_residues
MKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTLQTRFGIASGCKIFTAIGVCQLVENGVITF
QTKLKECLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYAPGSKFHY
NNAGFIILGLIIEEQTGLKFTEYIEKNIFNPIGMNHSGYFSLNRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSDGGAF
ITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKVFKYHVMGYDPGVSFRSAVYPDLGITLV
IPSNKGAGPEKLMIEIEGAF
>Mature_340_residues
MKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTLQTRFGIASGCKIFTAIGVCQLVENGVITF
QTKLKECLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIMDNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYAPGSKFHY
NNAGFIILGLIIEEQTGLKFTEYIEKNIFNPIGMNHSGYFSLNRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSDGGAF
ITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKVFKYHVMGYDPGVSFRSAVYPDLGITLV
IPSNKGAGPEKLMIEIEGAF

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI115533026, Length=324, Percent_Identity=25.6172839506173, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17550934, Length=180, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17565630, Length=289, Percent_Identity=23.8754325259516, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 38833; Mature: 38833

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTLQTRFGIASG
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEHHHCCCCCCHHHHHHHHHHHHCCCCC
CKIFTAIGVCQLVENGVITFQTKLKECLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIM
CHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHH
DNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYAPGSKFHYNNAGFIILGLIIEEQTGLKF
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCEEEEEEEEECCCCCHH
TEYIEKNIFNPIGMNHSGYFSLNRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSDGGAF
HHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCE
ITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKVFKYHVMG
EEHHHHHHHHHHHHCHHHHCCCCEEEEEECEEEECCCCCCEEEEEEEECCCEEEEEEEEE
YDPGVSFRSAVYPDLGITLVIPSNKGAGPEKLMIEIEGAF
ECCCCCHHHHCCCCCCEEEEEECCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MKTKDQIDNIVKETYRHIDFSGVILVKEKKGIVYEEAFGYANRNECINNTLQTRFGIASG
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEHHHCCCCCCHHHHHHHHHHHHCCCCC
CKIFTAIGVCQLVENGVITFQTKLKECLSVNFPNFNEDITIHHLLTHSSGIPDYFDESIM
CHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHH
DNFEDLWKQTPMYLLKSLKDFLPLFQNRDMMYAPGSKFHYNNAGFIILGLIIEEQTGLKF
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCEEEEEEEEECCCCCHH
TEYIEKNIFNPIGMNHSGYFSLNRLPRQTALGYIKDEISQTWRTNAYSIPIKGGSDGGAF
HHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCE
ITASDMLKFWEALFNYEIISQEYTKILLTPHIQVNNNQSYGYGIWIETRENKVFKYHVMG
EEHHHHHHHHHHHHCHHHHCCCCEEEEEECEEEECCCCCCEEEEEEEECCCEEEEEEEEE
YDPGVSFRSAVYPDLGITLVIPSNKGAGPEKLMIEIEGAF
ECCCCCHHHHCCCCCCEEEEEECCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]