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The map label for this gene is fruB [H]

Identifier: 52141769

GI number: 52141769

Start: 3602503

End: 3603414

Strand: Reverse

Name: fruB [H]

Synonym: BCZK3477

Alternate gene names: 52141769

Gene position: 3603414-3602503 (Counterclockwise)

Preceding gene: 52141770

Following gene: 52141771

Centisome position: 67.98

GC content: 35.53

Gene sequence:

>912_bases
ATGATCTATACAGTAACTTTAAACCCATCTATTGATTATATAGTACAAGTTAATTCCTTAGAGTTAGGCGCAGTAAATCG
AGCAGAAAAAGATATGAAGTTTCCTGGAGGAAAAGGGATTAATGTTTCTCGTGTTCTTCATCGTTTAGGTGTTAAAAATG
TAGCGCTAGGATTTACTGGTGGATTTACCGGTCGATTTATTAAAGATGTATTACAGACGGAAGGTGTAATAACAAACTTC
ATCCAAGTAAATGAAGATACTCGAATTAATGTGAAAATAAAAGGGGAAGAAGAAACAGAATTAAATGGGCAAGGACCTAG
TGTGACCAATGAACAATTTGAACAATTAATGAAACAAATTGAAAGTATGCAAAAGGGAGATTGTGTTGTACTAGCTGGAA
GTGTACCAGCGTCTATTCCAACTACTTTTTATGAATCAATCGCAGCGTTCGGAGCTGAAAAAGGTATTCGTGTAGTAGTA
GATGCGAGCGGAAGTGCCTTGCAGCATGTAGTTAAAAATAAACCATTTTTAATAAAACCAAATCATCATGAACTTGGTGA
GTTATTTGGAGTAGAAATTTCAACAGCAGAAGATATTTTACCGTATGGAAGAAAATTAATTGAACAAGGTGTAGAGCATG
TTATCGTATCAATGGCTGGAGATGGAGCTTTATTATTTACGACAGAAGGTATATATGAAGCAACTGTTCCAAAAGGTGTT
GTAATTAATTCGGTTGGGGCTGGAGATTCTCTCGTTGCGGGATTTGTAGGTAAATATGAACAGACAAAAGATATTGAAAA
GGCATTTCAATATGGCGTTGCTACAGGGAGTGCAACAGCATTTTCAGCTGATTTATGTGAAAAAGAAAAAGTAGAAGAAT
TATTGTCGCAAGTAATTGTAACTAAGCGATAG

Upstream 100 bases:

>100_bases
AAATTGCCAATGTTGAAGAGGCAAATATTATTACAAACCATTTAGAAATTGATTTAGAAAAATATAAAAGACAAACCAAT
GTAATTGAGGCTGATAAACA

Downstream 100 bases:

>100_bases
AGGGGAAGCAACGTATGAAAATTACAGATCTATTAAAAAGGGATACAGTTATTATGAATTTGACAGCTTCAAATAAAGAA
GCTGTCATAGATGAATTAGT

Product: 1-phosphofructokinase

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIYTVTLNPSIDYIVQVNSLELGAVNRAEKDMKFPGGKGINVSRVLHRLGVKNVALGFTGGFTGRFIKDVLQTEGVITNF
IQVNEDTRINVKIKGEEETELNGQGPSVTNEQFEQLMKQIESMQKGDCVVLAGSVPASIPTTFYESIAAFGAEKGIRVVV
DASGSALQHVVKNKPFLIKPNHHELGELFGVEISTAEDILPYGRKLIEQGVEHVIVSMAGDGALLFTTEGIYEATVPKGV
VINSVGAGDSLVAGFVGKYEQTKDIEKAFQYGVATGSATAFSADLCEKEKVEELLSQVIVTKR

Sequences:

>Translated_303_residues
MIYTVTLNPSIDYIVQVNSLELGAVNRAEKDMKFPGGKGINVSRVLHRLGVKNVALGFTGGFTGRFIKDVLQTEGVITNF
IQVNEDTRINVKIKGEEETELNGQGPSVTNEQFEQLMKQIESMQKGDCVVLAGSVPASIPTTFYESIAAFGAEKGIRVVV
DASGSALQHVVKNKPFLIKPNHHELGELFGVEISTAEDILPYGRKLIEQGVEHVIVSMAGDGALLFTTEGIYEATVPKGV
VINSVGAGDSLVAGFVGKYEQTKDIEKAFQYGVATGSATAFSADLCEKEKVEELLSQVIVTKR
>Mature_303_residues
MIYTVTLNPSIDYIVQVNSLELGAVNRAEKDMKFPGGKGINVSRVLHRLGVKNVALGFTGGFTGRFIKDVLQTEGVITNF
IQVNEDTRINVKIKGEEETELNGQGPSVTNEQFEQLMKQIESMQKGDCVVLAGSVPASIPTTFYESIAAFGAEKGIRVVV
DASGSALQHVVKNKPFLIKPNHHELGELFGVEISTAEDILPYGRKLIEQGVEHVIVSMAGDGALLFTTEGIYEATVPKGV
VINSVGAGDSLVAGFVGKYEQTKDIEKAFQYGVATGSATAFSADLCEKEKVEELLSQVIVTKR

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=300, Percent_Identity=28.6666666666667, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1788493, Length=259, Percent_Identity=32.046332046332, Blast_Score=128, Evalue=5e-31,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 32654; Mature: 32654

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTVTLNPSIDYIVQVNSLELGAVNRAEKDMKFPGGKGINVSRVLHRLGVKNVALGFTG
CEEEEEECCCEEEEEEECCEEECCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCEEEEECC
GFTGRFIKDVLQTEGVITNFIQVNEDTRINVKIKGEEETELNGQGPSVTNEQFEQLMKQI
CCHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
ESMQKGDCVVLAGSVPASIPTTFYESIAAFGAEKGIRVVVDASGSALQHVVKNKPFLIKP
HHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCCEEECC
NHHELGELFGVEISTAEDILPYGRKLIEQGVEHVIVSMAGDGALLFTTEGIYEATVPKGV
CCHHHHHHHCCEECCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCEECCCCCCE
VINSVGAGDSLVAGFVGKYEQTKDIEKAFQYGVATGSATAFSADLCEKEKVEELLSQVIV
EEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TKR
CCC
>Mature Secondary Structure
MIYTVTLNPSIDYIVQVNSLELGAVNRAEKDMKFPGGKGINVSRVLHRLGVKNVALGFTG
CEEEEEECCCEEEEEEECCEEECCCCCCHHHCCCCCCCCCCHHHHHHHHCCCCEEEEECC
GFTGRFIKDVLQTEGVITNFIQVNEDTRINVKIKGEEETELNGQGPSVTNEQFEQLMKQI
CCHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
ESMQKGDCVVLAGSVPASIPTTFYESIAAFGAEKGIRVVVDASGSALQHVVKNKPFLIKP
HHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCCEEECC
NHHELGELFGVEISTAEDILPYGRKLIEQGVEHVIVSMAGDGALLFTTEGIYEATVPKGV
CCHHHHHHHCCEECCHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCEECCCCCCE
VINSVGAGDSLVAGFVGKYEQTKDIEKAFQYGVATGSATAFSADLCEKEKVEELLSQVIV
EEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TKR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]