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The map label for this gene is exoA [H]

Identifier: 52141752

GI number: 52141752

Start: 3621447

End: 3622205

Strand: Reverse

Name: exoA [H]

Synonym: BCZK3496

Alternate gene names: 52141752

Gene position: 3622205-3621447 (Counterclockwise)

Preceding gene: 52141747

Following gene: 52141751

Centisome position: 68.33

GC content: 33.33

Gene sequence:

>759_bases
GTGAAGTTCATTTCGTGGAATGTAAATGGTTTACGTGCAGTTATCGCAAAAGGTGGATTTTTAGAATATCTAGAGGAATC
AAATGCTGATATATTTTGTTTACAAGAGATTAAATTACAAGAGGGGCAAATTGATTTAAATGTAGAGGACTATTATACAT
ACTGGAATTATGCTGTGAAAAAAGGATATTCAGGGACAGCTATTTTTTCGAAAAAAGAACCGCTTTCTGTTACATACGGT
TTAGGCATTGAAGAGCATGATCAAGAAGGACGAGTTATTACTTTAGAGTTCGAAGGTTTTTACATCATTACATTATATAC
ACCAAATGCAAAACGAGGATTAGAACGATTAGATTACAGAATGAAATGGGAAGATGATTTCCGGGCGTATATTAAACGAT
TAGATGAGAAAAAACCAGTTATTTTTTGCGGTGATTTAAACGTTGCCCATAAAGAAATAGATTTGAAAAATCCAAAAAGT
AATCGTAAAAACCCTGGGTTTTCTGATGAAGAAAGAGAGAAGTTTACATGTATTTTAGAAGAAGGATTTATTGATACGTA
TCGTTATCTATATCCTGATCAGGAAGGCGCATATTCGTGGTGGTCGTATCGAATGGGAGCAAGAGCAAAAAATATTGGAT
GGCGTTTAGATTATTTTGTTGTCTCCGAGAGAATAAAGAATCAAATAAAAGATGCGAAAATTAATAGTGAAGTAATGGGA
TCAGACCATTGCCCAGTTGAATTACATATAAATTTCTAA

Upstream 100 bases:

>100_bases
CATAAATGTAAGAGCAAGATTACAAAGGTGAAAATATGCTTGTTTTTCTATTTTGTGATAGCATGAAATTTGGAAAGAAA
CATTAAGAGGAGGACTATAA

Downstream 100 bases:

>100_bases
AAAAGAAGTATTCACCTAACATTAGGTGGATGCTTCTTTTTTATTCAGTTTGTTAAAATTTTTCAAATCTTTCTTGAAAT
AATTGCATATTAAATATATA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKFISWNVNGLRAVIAKGGFLEYLEESNADIFCLQEIKLQEGQIDLNVEDYYTYWNYAVKKGYSGTAIFSKKEPLSVTYG
LGIEEHDQEGRVITLEFEGFYIITLYTPNAKRGLERLDYRMKWEDDFRAYIKRLDEKKPVIFCGDLNVAHKEIDLKNPKS
NRKNPGFSDEEREKFTCILEEGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERIKNQIKDAKINSEVMG
SDHCPVELHINF

Sequences:

>Translated_252_residues
MKFISWNVNGLRAVIAKGGFLEYLEESNADIFCLQEIKLQEGQIDLNVEDYYTYWNYAVKKGYSGTAIFSKKEPLSVTYG
LGIEEHDQEGRVITLEFEGFYIITLYTPNAKRGLERLDYRMKWEDDFRAYIKRLDEKKPVIFCGDLNVAHKEIDLKNPKS
NRKNPGFSDEEREKFTCILEEGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERIKNQIKDAKINSEVMG
SDHCPVELHINF
>Mature_252_residues
MKFISWNVNGLRAVIAKGGFLEYLEESNADIFCLQEIKLQEGQIDLNVEDYYTYWNYAVKKGYSGTAIFSKKEPLSVTYG
LGIEEHDQEGRVITLEFEGFYIITLYTPNAKRGLERLDYRMKWEDDFRAYIKRLDEKKPVIFCGDLNVAHKEIDLKNPKS
NRKNPGFSDEEREKFTCILEEGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERIKNQIKDAKINSEVMG
SDHCPVELHINF

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=258, Percent_Identity=48.4496124031008, Blast_Score=272, Evalue=2e-73,
Organism=Homo sapiens, GI18375503, Length=258, Percent_Identity=48.4496124031008, Blast_Score=272, Evalue=2e-73,
Organism=Homo sapiens, GI18375501, Length=258, Percent_Identity=48.4496124031008, Blast_Score=272, Evalue=2e-73,
Organism=Homo sapiens, GI18375507, Length=313, Percent_Identity=31.629392971246, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1788046, Length=264, Percent_Identity=26.8939393939394, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71989536, Length=256, Percent_Identity=47.65625, Blast_Score=226, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI71989539, Length=145, Percent_Identity=38.6206896551724, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI221330655, Length=255, Percent_Identity=50.1960784313725, Blast_Score=270, Evalue=8e-73,
Organism=Drosophila melanogaster, GI17136678, Length=255, Percent_Identity=50.1960784313725, Blast_Score=270, Evalue=9e-73,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29639; Mature: 29639

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFISWNVNGLRAVIAKGGFLEYLEESNADIFCLQEIKLQEGQIDLNVEDYYTYWNYAVK
CEEEEECCCHHEEEECCCCHHHHHHCCCCCEEEEEEEEECCCEEEEEHHHHEEEEEHHHH
KGYSGTAIFSKKEPLSVTYGLGIEEHDQEGRVITLEFEGFYIITLYTPNAKRGLERLDYR
CCCCCCEEEECCCCCEEEEECCCCCCCCCCCEEEEEECCEEEEEEECCCHHHHHHHCCEE
MKWEDDFRAYIKRLDEKKPVIFCGDLNVAHKEIDLKNPKSNRKNPGFSDEEREKFTCILE
ECCCHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEE
EGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERIKNQIKDAKINSEVMG
CCCHHHHHEECCCCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCHHCC
SDHCPVELHINF
CCCCCEEEEECC
>Mature Secondary Structure
MKFISWNVNGLRAVIAKGGFLEYLEESNADIFCLQEIKLQEGQIDLNVEDYYTYWNYAVK
CEEEEECCCHHEEEECCCCHHHHHHCCCCCEEEEEEEEECCCEEEEEHHHHEEEEEHHHH
KGYSGTAIFSKKEPLSVTYGLGIEEHDQEGRVITLEFEGFYIITLYTPNAKRGLERLDYR
CCCCCCEEEECCCCCEEEEECCCCCCCCCCCEEEEEECCEEEEEEECCCHHHHHHHCCEE
MKWEDDFRAYIKRLDEKKPVIFCGDLNVAHKEIDLKNPKSNRKNPGFSDEEREKFTCILE
ECCCHHHHHHHHHHCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEE
EGFIDTYRYLYPDQEGAYSWWSYRMGARAKNIGWRLDYFVVSERIKNQIKDAKINSEVMG
CCCHHHHHEECCCCCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCHHCC
SDHCPVELHINF
CCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]