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The map label for this gene is colA [H]

Identifier: 52141733

GI number: 52141733

Start: 3638153

End: 3639994

Strand: Reverse

Name: colA [H]

Synonym: BCZK3514

Alternate gene names: 52141733

Gene position: 3639994-3638153 (Counterclockwise)

Preceding gene: 52141729

Following gene: 52141734

Centisome position: 68.67

GC content: 34.69

Gene sequence:

>1842_bases
ATGAAAATATTTCAAAAAATGTGTGCGTCAACTGTAATCATTACAACACTTTTAGGAATAGGAGGTACTGTCAATAGTAT
ACATGCTGATTCTATAGAACAGCAGAGTAAATTAGGAATAGACCAAGAAACAGATAAGCAAATCATTGTAAAATTTAAAG
CGGGTTTGAAATTTCCATATGAAGATGGTATTGAAAAACAAATACAATCTCAAACGAACGACAAAGTACTAGAAGATCTT
TTGGTAGAATATCCAGATCTAACATTCACGCGTTTATTCACATCTGTAAGTCCAGAGGAGATAGAAGACCTTGAAGTGAA
AGCACCCAATAATGTATCTACAAGTTTATTAAACTACTATATTCTTCAAACTCAAAATGGTGAGCAGAAAGAAGAACTAG
TAGATAGGTTAAAGAAATCTTCTTTAATAGAAGATGTGTATATGAAGAAACAAGAAAAAATAACGCCACCAGAAGCGCAA
CCATCAAGTGTATCTCTTAGTCCTAATAACAATCCTAGATTTAAACAACAAGGTTATTTAGAAGCAGCACCATACGGTAT
TAATGCGCCCTTCGCCTGGGAAGTTCAAGGCGGTAAAGGAAGTGGTATAACTTTTGTTGATATGGAATATGGATGGTTAT
TAAATCATGAGGATTTAGTACATCAAAATATTGAATTAATGTCTGGAATAAATATAGATCAACATGTCGGTCATGGGACG
TCTGTACTAGGAATTGTATCCTCTGAGGACAATGAAGTTGGGAATATTGGGATTGCGCCAAAAGCGAAAGCAAAGGTGAT
TTCTCAAATAAGAGATAACGGACAGTATAATACAGCTGATGCAATATTAAGTGCTGTGAATCAGTTAGAAGCTGGGGATG
TTTTATTATTAGAGGCACAAGCTTCCTTTGATGGATATGGTGATAAATATTTACCTGTTGAGGTACAACCAGATATCTTT
GATGCAATTCGTGCCGGTACAGATAAAGGTGTTGTCATTATAGAAGCTGGAGCAAATGGTTGGAATGATTTAGACCAATT
TAAAGATAGAAATGGTAAACAAATTTTAAATCGTAATAGTAAAGATTTTAAAGATTCAGGTGCAATTATGGTAGGAGCGG
GCTCTTCATCTTTCCCTCATGAGCGTATGTGGTTTTCGAATTATGGCAGCCGTATTGATGTGTATGGATGGGGAGAAAAC
GTAGATACAACTACAGCAGAGCAAAGTCGAAGTGCTGTAAATCTTTATACATCTAGCTTTAGTGGCACGTCGAGTGCGTC
ACCTATTATTGCCGGAGCAGCGACTTTAGTACAAAGCATTGCGAAAGAAAATTTAGGACAACCATATAGACCGAGTGAAC
TAAGAGCAATATTAAGCAACTACAACACAGGAACAAAATCTAAAGATCCATATGCAGATAAAATTGGTGTTTTACCAGAT
TTGAAGTCTATACTAGTTAACTTAGGGTATGGACAAAGAAAACCAAACGGTGGGAATGAGTTACAAGTAACAGAAAATGA
ACCAAATAATGAACGTATACAGGCAAATAAAGTTAACTTTCATACACCGGTGAAAGGAACGTTACATAATAGTGATAGAG
TAGATGTATTTACTTTCCAAATTGACTCTATGGAAAATATTAGCATATCCCTACTAAATGAACAAAATATCGGAATGACA
TGGGTACTTCATCATGAATCAGATTTAAATAACTATGTAGCATATGGTGAAAATGAAGGAAATGTAGTGAAAGGAACTTA
TAATGCAAAGCCAGGTAAATATTATTTATACGTCTATAAGTATGAGAATAAAGATGGTTCATATGTATTGAATATAAAGT
AA

Upstream 100 bases:

>100_bases
ATTACTATAATCTATTTAGTAGAAAGGGAATATGTTATACAAACAAAATCATTATAAATTAATATAATGATTAATTGTTG
TTAATAGGAGGATGGATGTA

Downstream 100 bases:

>100_bases
ACGAAAGGAGCTTCAGATGAAGTTCCTTTAAATTTTTCTAATTCAGGATATATGAATGTTTTTTAACTTTATTTCTATGC
AATATTTCATACTTATGAAA

Product: serine protease

Products: NA

Alternate protein names: 120 kDa collagenase [H]

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MKIFQKMCASTVIITTLLGIGGTVNSIHADSIEQQSKLGIDQETDKQIIVKFKAGLKFPYEDGIEKQIQSQTNDKVLEDL
LVEYPDLTFTRLFTSVSPEEIEDLEVKAPNNVSTSLLNYYILQTQNGEQKEELVDRLKKSSLIEDVYMKKQEKITPPEAQ
PSSVSLSPNNNPRFKQQGYLEAAPYGINAPFAWEVQGGKGSGITFVDMEYGWLLNHEDLVHQNIELMSGINIDQHVGHGT
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQASFDGYGDKYLPVEVQPDIF
DAIRAGTDKGVVIIEAGANGWNDLDQFKDRNGKQILNRNSKDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGEN
VDTTTAEQSRSAVNLYTSSFSGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNYNTGTKSKDPYADKIGVLPD
LKSILVNLGYGQRKPNGGNELQVTENEPNNERIQANKVNFHTPVKGTLHNSDRVDVFTFQIDSMENISISLLNEQNIGMT
WVLHHESDLNNYVAYGENEGNVVKGTYNAKPGKYYLYVYKYENKDGSYVLNIK

Sequences:

>Translated_613_residues
MKIFQKMCASTVIITTLLGIGGTVNSIHADSIEQQSKLGIDQETDKQIIVKFKAGLKFPYEDGIEKQIQSQTNDKVLEDL
LVEYPDLTFTRLFTSVSPEEIEDLEVKAPNNVSTSLLNYYILQTQNGEQKEELVDRLKKSSLIEDVYMKKQEKITPPEAQ
PSSVSLSPNNNPRFKQQGYLEAAPYGINAPFAWEVQGGKGSGITFVDMEYGWLLNHEDLVHQNIELMSGINIDQHVGHGT
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQASFDGYGDKYLPVEVQPDIF
DAIRAGTDKGVVIIEAGANGWNDLDQFKDRNGKQILNRNSKDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGEN
VDTTTAEQSRSAVNLYTSSFSGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNYNTGTKSKDPYADKIGVLPD
LKSILVNLGYGQRKPNGGNELQVTENEPNNERIQANKVNFHTPVKGTLHNSDRVDVFTFQIDSMENISISLLNEQNIGMT
WVLHHESDLNNYVAYGENEGNVVKGTYNAKPGKYYLYVYKYENKDGSYVLNIK
>Mature_613_residues
MKIFQKMCASTVIITTLLGIGGTVNSIHADSIEQQSKLGIDQETDKQIIVKFKAGLKFPYEDGIEKQIQSQTNDKVLEDL
LVEYPDLTFTRLFTSVSPEEIEDLEVKAPNNVSTSLLNYYILQTQNGEQKEELVDRLKKSSLIEDVYMKKQEKITPPEAQ
PSSVSLSPNNNPRFKQQGYLEAAPYGINAPFAWEVQGGKGSGITFVDMEYGWLLNHEDLVHQNIELMSGINIDQHVGHGT
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQASFDGYGDKYLPVEVQPDIF
DAIRAGTDKGVVIIEAGANGWNDLDQFKDRNGKQILNRNSKDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGEN
VDTTTAEQSRSAVNLYTSSFSGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNYNTGTKSKDPYADKIGVLPD
LKSILVNLGYGQRKPNGGNELQVTENEPNNERIQANKVNFHTPVKGTLHNSDRVDVFTFQIDSMENISISLLNEQNIGMT
WVLHHESDLNNYVAYGENEGNVVKGTYNAKPGKYYLYVYKYENKDGSYVLNIK

Specific function: Unknown

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PKD domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013510
- InterPro:   IPR007280
- InterPro:   IPR002169
- InterPro:   IPR013661
- InterPro:   IPR022409
- InterPro:   IPR000601 [H]

Pfam domain/function: PF01752 Peptidase_M9; PF08453 Peptidase_M9_N; PF00801 PKD; PF04151 PPC [H]

EC number: =3.4.24.3 [H]

Molecular weight: Translated: 67716; Mature: 67716

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFQKMCASTVIITTLLGIGGTVNSIHADSIEQQSKLGIDQETDKQIIVKFKAGLKFPY
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCH
EDGIEKQIQSQTNDKVLEDLLVEYPDLTFTRLFTSVSPEEIEDLEVKAPNNVSTSLLNYY
HCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCEEEECCCCHHHHEEEEE
ILQTQNGEQKEELVDRLKKSSLIEDVYMKKQEKITPPEAQPSSVSLSPNNNPRFKQQGYL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCE
EAAPYGINAPFAWEVQGGKGSGITFVDMEYGWLLNHEDLVHQNIELMSGINIDQHVGHGT
EECCCCCCCCEEEEEECCCCCCEEEEEECCCEEECCHHHHHHCHHHHHCCCHHHHCCCCC
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQ
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEE
ASFDGYGDKYLPVEVQPDIFDAIRAGTDKGVVIIEAGANGWNDLDQFKDRNGKQILNRNS
CCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCCHHHHCCCC
KDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGENVDTTTAEQSRSAVNLYTSSF
CCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCEEEEEECCC
SGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNYNTGTKSKDPYADKIGVLPD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCC
LKSILVNLGYGQRKPNGGNELQVTENEPNNERIQANKVNFHTPVKGTLHNSDRVDVFTFQ
HHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCEECCCCCEEEEEEE
IDSMENISISLLNEQNIGMTWVLHHESDLNNYVAYGENEGNVVKGTYNAKPGKYYLYVYK
ECCCCCCEEEEEECCCCCEEEEEEECCCCCCEEEECCCCCCEEEEEECCCCCEEEEEEEE
YENKDGSYVLNIK
EECCCCCEEEEEC
>Mature Secondary Structure
MKIFQKMCASTVIITTLLGIGGTVNSIHADSIEQQSKLGIDQETDKQIIVKFKAGLKFPY
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCH
EDGIEKQIQSQTNDKVLEDLLVEYPDLTFTRLFTSVSPEEIEDLEVKAPNNVSTSLLNYY
HCCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHCCEEEECCCCHHHHEEEEE
ILQTQNGEQKEELVDRLKKSSLIEDVYMKKQEKITPPEAQPSSVSLSPNNNPRFKQQGYL
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCE
EAAPYGINAPFAWEVQGGKGSGITFVDMEYGWLLNHEDLVHQNIELMSGINIDQHVGHGT
EECCCCCCCCEEEEEECCCCCCEEEEEECCCEEECCHHHHHHCHHHHHCCCHHHHCCCCC
SVLGIVSSEDNEVGNIGIAPKAKAKVISQIRDNGQYNTADAILSAVNQLEAGDVLLLEAQ
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEE
ASFDGYGDKYLPVEVQPDIFDAIRAGTDKGVVIIEAGANGWNDLDQFKDRNGKQILNRNS
CCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHCCCCHHHHCCCC
KDFKDSGAIMVGAGSSSFPHERMWFSNYGSRIDVYGWGENVDTTTAEQSRSAVNLYTSSF
CCCCCCCCEEEECCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCHHHCCCEEEEEECCC
SGTSSASPIIAGAATLVQSIAKENLGQPYRPSELRAILSNYNTGTKSKDPYADKIGVLPD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHCCCCCC
LKSILVNLGYGQRKPNGGNELQVTENEPNNERIQANKVNFHTPVKGTLHNSDRVDVFTFQ
HHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCEECCCCCEEEEEEE
IDSMENISISLLNEQNIGMTWVLHHESDLNNYVAYGENEGNVVKGTYNAKPGKYYLYVYK
ECCCCCCEEEEEECCCCCEEEEEEECCCCCCEEEECCCCCCEEEEEECCCCCEEEEEEEE
YENKDGSYVLNIK
EECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8282691; 11792842 [H]