Click here to switch to the map view.

The map label for this gene is prpB [C]

Identifier: 52141722

GI number: 52141722

Start: 3654350

End: 3655174

Strand: Reverse

Name: prpB [C]

Synonym: BCZK3525

Alternate gene names: 52141722

Gene position: 3655174-3654350 (Counterclockwise)

Preceding gene: 52141721

Following gene: 52141727

Centisome position: 68.95

GC content: 32.73

Gene sequence:

>825_bases
ATGAATGACATTTTAATGAAGTCAAAGATATTTAATGATTATCATCACGATTCTTCCATATTAATTTTACCAAACGCTTG
GGATGTTATGAGTGCAAAGATATATGAAGACTTAAACTATAGAGCTATTGGAACAACTAGTGCAGGAATTGCGGCATCTT
TAGGTTATAGTGATGGTGAGCAACTGCCATTTGAATCCATGCTTGATGTTATTGAGAAAATTACCCAATCTGTAAATATC
CCTGTCAGTGCAGATATAGAATCTGGATATGGAGAAACAATTGAAGAAGTATTAGAGAATGTAAGAAAAATTATAGCTAG
CGGTGTTGTAGGGATAAACTTAGAAGATAGTAAGAAAAATCATGTATATTCCTTATACGATACGGCATATCAGCAAAAGA
AAATTGAGAGTATTAAAAATGTGTCTATTTCAGAAGGGGTTCCTCTCTTTATAAATGCACGTACAGATGCATACCTACTT
AACAATAATAGATTTGAAGAGACGATGAAGCGTGCACAAGCATACAAAGATGCTGGCGCAGATGGAATTTTCATCCCTGG
ATTAGCTAAGAAAGAACATATTCACATGTTTACTGAAAAAATAAATTTACCACTTAATGTTTTGGTTAATAACTCTACAC
CATCTATTCTAGATTTAGAATATTTAAATGTTAGTAGAATAAGTTTTGGATCTGGAGCATATAGAGCTACTATTTCTACT
TTACGAAAACTTGCATCGGAAGTAATTACGAAAGGGCAGTATGAAACCATGACTAATGAAGTTATGTCCTATGAAAATAT
GGTGGGATTTCTTCATAAAAACTAA

Upstream 100 bases:

>100_bases
TCATTTTTCACACTCCTAATAAAATAAGTATATTTCCATGCTAATCTAATTTTCAAAATGGTAAAATTGTTACAAGATAT
TATTTTAGGGGGAAGATAAA

Downstream 100 bases:

>100_bases
GTGATATTTAATTTAAGTGTTTATATTTTTAGTTGAGTAGGCTGTTGCAAAGCAGTTAGTTAAACAAAAACCGATTTATC
CCTAGGTAAATCGGTTTTTG

Product: carboxyphosphonoenolpyruvate phosphonomutase

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNDILMKSKIFNDYHHDSSILILPNAWDVMSAKIYEDLNYRAIGTTSAGIAASLGYSDGEQLPFESMLDVIEKITQSVNI
PVSADIESGYGETIEEVLENVRKIIASGVVGINLEDSKKNHVYSLYDTAYQQKKIESIKNVSISEGVPLFINARTDAYLL
NNNRFEETMKRAQAYKDAGADGIFIPGLAKKEHIHMFTEKINLPLNVLVNNSTPSILDLEYLNVSRISFGSGAYRATIST
LRKLASEVITKGQYETMTNEVMSYENMVGFLHKN

Sequences:

>Translated_274_residues
MNDILMKSKIFNDYHHDSSILILPNAWDVMSAKIYEDLNYRAIGTTSAGIAASLGYSDGEQLPFESMLDVIEKITQSVNI
PVSADIESGYGETIEEVLENVRKIIASGVVGINLEDSKKNHVYSLYDTAYQQKKIESIKNVSISEGVPLFINARTDAYLL
NNNRFEETMKRAQAYKDAGADGIFIPGLAKKEHIHMFTEKINLPLNVLVNNSTPSILDLEYLNVSRISFGSGAYRATIST
LRKLASEVITKGQYETMTNEVMSYENMVGFLHKN
>Mature_274_residues
MNDILMKSKIFNDYHHDSSILILPNAWDVMSAKIYEDLNYRAIGTTSAGIAASLGYSDGEQLPFESMLDVIEKITQSVNI
PVSADIESGYGETIEEVLENVRKIIASGVVGINLEDSKKNHVYSLYDTAYQQKKIESIKNVSISEGVPLFINARTDAYLL
NNNRFEETMKRAQAYKDAGADGIFIPGLAKKEHIHMFTEKINLPLNVLVNNSTPSILDLEYLNVSRISFGSGAYRATIST
LRKLASEVITKGQYETMTNEVMSYENMVGFLHKN

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=214, Percent_Identity=25.7009345794392, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 30593; Mature: 30593

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDILMKSKIFNDYHHDSSILILPNAWDVMSAKIYEDLNYRAIGTTSAGIAASLGYSDGE
CCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEECCCCCCCEEECCCCCCC
QLPFESMLDVIEKITQSVNIPVSADIESGYGETIEEVLENVRKIIASGVVGINLEDSKKN
CCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCC
HVYSLYDTAYQQKKIESIKNVSISEGVPLFINARTDAYLLNNNRFEETMKRAQAYKDAGA
CEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHCCC
DGIFIPGLAKKEHIHMFTEKINLPLNVLVNNSTPSILDLEYLNVSRISFGSGAYRATIST
CCEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCEEEEECCCCHHHHHHHH
LRKLASEVITKGQYETMTNEVMSYENMVGFLHKN
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDILMKSKIFNDYHHDSSILILPNAWDVMSAKIYEDLNYRAIGTTSAGIAASLGYSDGE
CCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCCCEEECCCCCCCEEECCCCCCC
QLPFESMLDVIEKITQSVNIPVSADIESGYGETIEEVLENVRKIIASGVVGINLEDSKKN
CCCHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCC
HVYSLYDTAYQQKKIESIKNVSISEGVPLFINARTDAYLLNNNRFEETMKRAQAYKDAGA
CEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEECCCHHHHHHHHHHHHHHCCC
DGIFIPGLAKKEHIHMFTEKINLPLNVLVNNSTPSILDLEYLNVSRISFGSGAYRATIST
CCEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCEEEEECCCCHHHHHHHH
LRKLASEVITKGQYETMTNEVMSYENMVGFLHKN
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]