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The map label for this gene is miaB [H]

Identifier: 52141716

GI number: 52141716

Start: 3661629

End: 3663158

Strand: Reverse

Name: miaB [H]

Synonym: BCZK3531

Alternate gene names: 52141716

Gene position: 3663158-3661629 (Counterclockwise)

Preceding gene: 52141715

Following gene: 52141718

Centisome position: 69.1

GC content: 36.6

Gene sequence:

>1530_bases
ATGAACGAGCAACAACGATTAGCAAGTCAACAAGTGAATTCCTCTACGAAAAAAGAAGAGAAAGATTATAGCAAATACTT
TGAAAGTGTATACCAACCACCTTCCTTAAAAGAGGCGAAAAAGCGAGGGAAAGAAGAAGTTAAAATTGAACGGGATTTTG
GCTTACCTGAAGAGTTTCGCAATTTCGGTACAGGAAGAAAGTTCTATATTCGTACATATGGTTGTCAAATGAATGAGCAT
GATACAGAAGTAATGGCTGGTATTTTTACTGCACTGGGATATGAACCAACCTTTTCAACTGAAGACGCAGATGTAGTATT
ATTAAATACTTGTGCCATCCGTGAAAATGCTGAAAATAAAGTGTTCGGTGAACTAGGTCATTTAAAGGCACTAAAAAGAC
GAAATCCAGACCTTTTAATTGGTGTATGTGGTTGTATGTCTCAAGAGGAATCTGTTGTAAATAAAATTATGCAAAAAAAT
CAGCATGTAGATATGGTGTTTGGAACGCATAATATTCATAGATTACCGTACATTCTAAAAGATGCAATGTTCTCGAAGGA
GACGGTAGTTGAAGTTTGGTCCAAAGAAGGAGACGTAATTGAAAACCTTCCGAAAGTACGCCGTGGTGATATTAAAGCTT
GGGTAAATATTATGTATGGCTGTGATAAGTTCTGTACATATTGTATCGTACCGTATACACGTGGAAAAGAGCGAAGCAGA
CGTCCAGAAGACATTATCCAAGAGATTCGTCATTTAGCTGCGAATGGTTATAAAGAAATTACGTTACTTGGACAGAACGT
AAATGCATATGGTAAAGACTTTGAAGATATAGAATACGGTCTTGGCGATTTAATGGACGAACTCCGTAAAGTTGATATTG
CCCGTATTCGTTTTACAACGAGCCATCCACGTGATTTCGATGATCACTTAATTGAAGTGCTTGGAAAAGGCGGAAACTTA
GTAGAACATATTCACTTACCAGTTCAATCTGGAAGCACAGAAATGTTAAAAATTATGGCGCGTAAATATTCACGTGAGCA
TTATTTAGAGCTTGTAAGAAAAATTAAAGAAGCAATTCCAAACGCGGTATTAACAACTGATATTATCGTCGGTTTCCCGA
ATGAAACAGATGAGCAATTTGAAGAAACGATGTCGTTATATCGTGAAGTAGGATTTGATACTGCCTTTACCTTTATTTAT
TCTCCGCGTGAAGGTACACCAGCTGCAAAAATGAAAGACAATGTACCGATGGAAGTAAAGAAAGAACGTCTTCAACGCTT
AAATGCATTAGTAAATAAATTGGCAATTGAAAAGAATGATCGTTATAAAGGTCAAATTGTAGAAGTGTTAGTTGATGGGG
AAAGTAAAAATAATCCAGAAGTACTTGCAGGTTATACTCGTACGAATAAACTTGTAAACTTCGTTGCTCCAAAGTCTTTA
ATTGGTCAGCTTGTGAAAGTAAAAGTAACGGATGCAAAAACTTGGTCTCTTAACGGAGAATTAGTTGAAGAGCCGATTGA
GGTGGAATAA

Upstream 100 bases:

>100_bases
CAAGTTATCTCTCAAGCTATCTAACCATTTTTGAGGTGGGTCTAAGCACATTAAAATGGACTATAGAGTGTGTATAGTTA
AGGGTGAAAGGAGATTACCA

Downstream 100 bases:

>100_bases
TAGATGAAAGTATATTCGAAAGATGAAATTGTTGAGCAAGCGAAAGAATTAGCAAAAATGATTTCTGAAACAGAAGAAGT
TGATTTCTTTAAGAAAGCGG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKEAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELGHLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
IGQLVKVKVTDAKTWSLNGELVEEPIEVE

Sequences:

>Translated_509_residues
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKEAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELGHLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
IGQLVKVKVTDAKTWSLNGELVEEPIEVE
>Mature_509_residues
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKEAKKRGKEEVKIERDFGLPEEFRNFGTGRKFYIRTYGCQMNEH
DTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELGHLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKN
QHVDMVFGTHNIHRLPYILKDAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTTSHPRDFDDHLIEVLGKGGNL
VEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIY
SPREGTPAAKMKDNVPMEVKKERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
IGQLVKVKVTDAKTWSLNGELVEEPIEVE

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=483, Percent_Identity=37.4741200828157, Blast_Score=308, Evalue=9e-84,
Organism=Homo sapiens, GI28872784, Length=483, Percent_Identity=37.4741200828157, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI93277076, Length=455, Percent_Identity=29.010989010989, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1786882, Length=440, Percent_Identity=45, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1787057, Length=454, Percent_Identity=27.7533039647577, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=505, Percent_Identity=34.2574257425743, Blast_Score=272, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI71996771, Length=307, Percent_Identity=31.2703583061889, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21356207, Length=484, Percent_Identity=37.1900826446281, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI19922432, Length=457, Percent_Identity=31.0722100656455, Blast_Score=192, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 58342; Mature: 58342

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKEAKKRGKEEVKIERDFGLPEEFR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHEEEECCCCCCHHHH
NFGTGRKFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENK
HCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCCH
VFGELGHLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILK
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
DAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHCHHHHHHHHHCCCCHHHHCHHHHHCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEEC
SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCC
NAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVK
CCEEEEHEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCHHH
IGQLVKVKVTDAKTWSLNGELVEEPIEVE
HHHHHEEEEECCEEEECCCHHHCCCCCCC
>Mature Secondary Structure
MNEQQRLASQQVNSSTKKEEKDYSKYFESVYQPPSLKEAKKRGKEEVKIERDFGLPEEFR
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHEEEECCCCCCHHHH
NFGTGRKFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENK
HCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEHHCCCCCCH
VFGELGHLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILK
HHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
DAMFSKETVVEVWSKEGDVIENLPKVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHCHHHHHHHHHCCCCHHHHCHHHHHCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVDIARIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEEC
SHPRDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIP
CCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCC
NAVLTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVK
CCEEEEHEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNALVNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSL
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCHHH
IGQLVKVKVTDAKTWSLNGELVEEPIEVE
HHHHHEEEEECCEEEECCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA