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The map label for this gene is wecC [H]

Identifier: 52140294

GI number: 52140294

Start: 5061552

End: 5062835

Strand: Reverse

Name: wecC [H]

Synonym: BCZK4967

Alternate gene names: 52140294

Gene position: 5062835-5061552 (Counterclockwise)

Preceding gene: 52140293

Following gene: 52140295

Centisome position: 95.51

GC content: 35.59

Gene sequence:

>1284_bases
ATGAAAATTTGTACAATGGGATTAGGTTATATTGGATTACCAACATCAGCAATGTTTGCAAAATATGGTACAGAAGTAGT
AGGTGTAGACATTCATCAAAAAGTAGTGGACAAGCTAAACAATGGTGAAATTCATATTGAAGAGCCAGGTCTTGGAGAAG
TTGTAAAAGAAGTAGTTGAAAATGGAAAATTTCGTGCATCTTTAACACCAGAAGAAGCAGATGCATTTATTATTTCTGTT
CCAACACCAAATAATGATGATGAACATAAATCTTGTGATTTAACGTATGTTTTAACAGCTACGAAAAAAGTATTGCCATT
TGTAAAAAAAGGAAATGTAATTATTGTTGAGTCTACAATTGCGCCACGTAGTATGGATGACCATGTAAAACCATTAGTAG
AAGAAGCTGGATTTACAGTTGGAGAAGATATTTATCTTGTTCACTGTCCAGAGCGCGTTCTTCCAGGACAAATTTTACAC
GAATTAGTACATAATAACCGTATTGTTGGTGGTATTACTCCTGCTTGCGCAGATGCTGGCGCTCGTGTATATGGTGTATT
TGTACAAGGTGAAATTATTAAAACAAATGCAAAAACAGCAGAAATGTCGAAGCTTATGGAAAATACATTCCGTGATGTAA
ATATTGCGCTTGCAAACGAGTTAACAAAAGTTTGTAATTCATTAGACATTAATGTATTAGATGTGATTGAAATGGCTAAC
AAGCATCCACGTGTAAACTTGCATTATCCAGGACCTGGTGTAGGTGGGCATTGCTTAGCAGTTGATCCATACTTTATCGT
GGCTAAAGCACCTGAATTGGCAAATATTATTAAACTTTCCCGTGAAACAAATGTTTCTATGCCACATTATGTGACTGAAA
AAGTAAAAGAATTACTTGCAGGTGTAGAAAATCCAAAAGTAGCGGCATTTGGTGTGACATATAAAGGTAATGTAGATGAC
ATGCGTGAAAGCCCAGCAATGGATGTAATTGACTTAATCCGTAACGAAGGGATTACAGTTTCAGCACATGATCCGCATGT
AGAATCTGACTTCTTTAAATTACATTCTGCAAAAGAAGCTGTTAAGGATGCGCATTTAATTCTTGTATTAACAGATCATG
ATGAATTTAAAACAATGAATTATGAAGCATTAGCACCACATATGGCGAAGGCCGTTGTTCTTGATACACGCAATTGTGTA
CAAGCTGTTTCAACTGCTGAAATGAAAGTCGTTAACTTTGGAACTTTATATGAGGCATTATCTGAAAACGTGATTACGGT
ATGA

Upstream 100 bases:

>100_bases
CAAAATCTATAGATTGTAGGCTAAATTAAATAAATTTTGGTTATATAAATCTATTGGATTATAGATAATAAATTTTTAGA
GAGACAGGAGAGGCATTAGA

Downstream 100 bases:

>100_bases
GCGCTATAAAAACTATTCTTAAGGAACAATATGAGAATATATATTTAATTCATAGGTTATCTCTTTATGAATTAAAACAA
GCATATGCAGGTAACTTATT

Product: N-acylmannosamine 1-dehydrogenase (UDP-N-acetyl-D-mannosamine dehydrogenase); capsular polysaccharide synthesis enzyme

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: UDP-ManNAc 6-dehydrogenase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKICTMGLGYIGLPTSAMFAKYGTEVVGVDIHQKVVDKLNNGEIHIEEPGLGEVVKEVVENGKFRASLTPEEADAFIISV
PTPNNDDEHKSCDLTYVLTATKKVLPFVKKGNVIIVESTIAPRSMDDHVKPLVEEAGFTVGEDIYLVHCPERVLPGQILH
ELVHNNRIVGGITPACADAGARVYGVFVQGEIIKTNAKTAEMSKLMENTFRDVNIALANELTKVCNSLDINVLDVIEMAN
KHPRVNLHYPGPGVGGHCLAVDPYFIVAKAPELANIIKLSRETNVSMPHYVTEKVKELLAGVENPKVAAFGVTYKGNVDD
MRESPAMDVIDLIRNEGITVSAHDPHVESDFFKLHSAKEAVKDAHLILVLTDHDEFKTMNYEALAPHMAKAVVLDTRNCV
QAVSTAEMKVVNFGTLYEALSENVITV

Sequences:

>Translated_427_residues
MKICTMGLGYIGLPTSAMFAKYGTEVVGVDIHQKVVDKLNNGEIHIEEPGLGEVVKEVVENGKFRASLTPEEADAFIISV
PTPNNDDEHKSCDLTYVLTATKKVLPFVKKGNVIIVESTIAPRSMDDHVKPLVEEAGFTVGEDIYLVHCPERVLPGQILH
ELVHNNRIVGGITPACADAGARVYGVFVQGEIIKTNAKTAEMSKLMENTFRDVNIALANELTKVCNSLDINVLDVIEMAN
KHPRVNLHYPGPGVGGHCLAVDPYFIVAKAPELANIIKLSRETNVSMPHYVTEKVKELLAGVENPKVAAFGVTYKGNVDD
MRESPAMDVIDLIRNEGITVSAHDPHVESDFFKLHSAKEAVKDAHLILVLTDHDEFKTMNYEALAPHMAKAVVLDTRNCV
QAVSTAEMKVVNFGTLYEALSENVITV
>Mature_427_residues
MKICTMGLGYIGLPTSAMFAKYGTEVVGVDIHQKVVDKLNNGEIHIEEPGLGEVVKEVVENGKFRASLTPEEADAFIISV
PTPNNDDEHKSCDLTYVLTATKKVLPFVKKGNVIIVESTIAPRSMDDHVKPLVEEAGFTVGEDIYLVHCPERVLPGQILH
ELVHNNRIVGGITPACADAGARVYGVFVQGEIIKTNAKTAEMSKLMENTFRDVNIALANELTKVCNSLDINVLDVIEMAN
KHPRVNLHYPGPGVGGHCLAVDPYFIVAKAPELANIIKLSRETNVSMPHYVTEKVKELLAGVENPKVAAFGVTYKGNVDD
MRESPAMDVIDLIRNEGITVSAHDPHVESDFFKLHSAKEAVKDAHLILVLTDHDEFKTMNYEALAPHMAKAVVLDTRNCV
QAVSTAEMKVVNFGTLYEALSENVITV

Specific function: Catalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA) [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4507813, Length=455, Percent_Identity=24.1758241758242, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI296040443, Length=444, Percent_Identity=23.6486486486486, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI296040438, Length=359, Percent_Identity=22.2841225626741, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI48994968, Length=400, Percent_Identity=46.25, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1788340, Length=373, Percent_Identity=25.201072386059, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560350, Length=439, Percent_Identity=27.5626423690205, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17136908, Length=441, Percent_Identity=25.3968253968254, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 46670; Mature: 46670

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKICTMGLGYIGLPTSAMFAKYGTEVVGVDIHQKVVDKLNNGEIHIEEPGLGEVVKEVVE
CEEEEECCCCCCCCHHHHHHHHCCEEEEECHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
NGKFRASLTPEEADAFIISVPTPNNDDEHKSCDLTYVLTATKKVLPFVKKGNVIIVESTI
CCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHEECCCEEEEECCC
APRSMDDHVKPLVEEAGFTVGEDIYLVHCPERVLPGQILHELVHNNRIVGGITPACADAG
CCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEECCCHHHHCCC
ARVYGVFVQGEIIKTNAKTAEMSKLMENTFRDVNIALANELTKVCNSLDINVLDVIEMAN
CEEEEEEEECEEEECCCCHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCCCHHHHHHHHHC
KHPRVNLHYPGPGVGGHCLAVDPYFIVAKAPELANIIKLSRETNVSMPHYVTEKVKELLA
CCCEEEEECCCCCCCCEEEEECCEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
GVENPKVAAFGVTYKGNVDDMRESPAMDVIDLIRNEGITVSAHDPHVESDFFKLHSAKEA
CCCCCCEEEEEEEEECCHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHH
VKDAHLILVLTDHDEFKTMNYEALAPHMAKAVVLDTRNCVQAVSTAEMKVVNFGTLYEAL
HCCCEEEEEEECCCCCEECCHHHHCHHHHHEEEEECHHHHHHHHHHHEEEEEHHHHHHHH
SENVITV
CCCCCCC
>Mature Secondary Structure
MKICTMGLGYIGLPTSAMFAKYGTEVVGVDIHQKVVDKLNNGEIHIEEPGLGEVVKEVVE
CEEEEECCCCCCCCHHHHHHHHCCEEEEECHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
NGKFRASLTPEEADAFIISVPTPNNDDEHKSCDLTYVLTATKKVLPFVKKGNVIIVESTI
CCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEECHHHHHHHEECCCEEEEECCC
APRSMDDHVKPLVEEAGFTVGEDIYLVHCPERVLPGQILHELVHNNRIVGGITPACADAG
CCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEECCCHHHHCCC
ARVYGVFVQGEIIKTNAKTAEMSKLMENTFRDVNIALANELTKVCNSLDINVLDVIEMAN
CEEEEEEEECEEEECCCCHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCCCHHHHHHHHHC
KHPRVNLHYPGPGVGGHCLAVDPYFIVAKAPELANIIKLSRETNVSMPHYVTEKVKELLA
CCCEEEEECCCCCCCCEEEEECCEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
GVENPKVAAFGVTYKGNVDDMRESPAMDVIDLIRNEGITVSAHDPHVESDFFKLHSAKEA
CCCCCCEEEEEEEEECCHHHHHCCCCHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHH
VKDAHLILVLTDHDEFKTMNYEALAPHMAKAVVLDTRNCVQAVSTAEMKVVNFGTLYEAL
HCCCEEEEEEECCCCCEECCHHHHCHHHHHEEEEECHHHHHHHHHHHEEEEEHHHHHHHH
SENVITV
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA