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The map label for this gene is wciJ [H]

Identifier: 52140288

GI number: 52140288

Start: 5071828

End: 5073069

Strand: Reverse

Name: wciJ [H]

Synonym: BCZK4973

Alternate gene names: 52140288

Gene position: 5073069-5071828 (Counterclockwise)

Preceding gene: 52140287

Following gene: 52140290

Centisome position: 95.7

GC content: 31.96

Gene sequence:

>1242_bases
ATGAAGAAAATTTTAATAGTAACGCAAAACTTTTATCCGGAAATAGGCAGTGCTGCTAATAGGATGAAAAGTGTATACTT
AGAATTAAAAGAAAGAGGTTATGACGTCACGGTATTAACTTCTGATCCCAGCTATCCAAATCGTAATTTATACAAAGATC
CAAATTATTGGTATGATGAGGATATAGAAAAAGATACAGAACGTATTCATCCGAAAACACGGAAGTATACAAGGAATCTT
TTTAATAGATTGATACTATACATAGAAGTTGCTCTCAGATTAATTTTAGCAATATGTAAAGACAAAAAGAAATATGATTA
TGTATTTGTTTCCACACCATCAATTTTTATTGCTGTAGCGGGTATGTTTGCTAAAAGAAAAATGAAAACGAAATTAATTT
TAGATGTACGGGATTTGTGGCCAGAATCATTGGTTGGCATAGGTTTTTTTGATAGAAAATGGATATTGAAGTTCGCTTAT
AAATTAGAGCGTAAAATATATCACTCAGCCGATAAAATTATTATTAATAGCCACGGATTTCTTCCTTATATTACCTCTAA
AGGCATTCCTATGAATCGTATCTCATTTATGCCTAATTCTTTAACAGAAGAAGAGCTATCTATAGTTCAAAAGGAGAACA
GTAGTAATCAGTTAAGGGTTATTTATACGGGAAATATAGGATTGGCACAGGATATAAAAAAATTAATTTCGATTGCAGAG
TACTTAAAAGACTATGAGAATATCTCATTTAAAATTATTGGATATGGTTATCAGAGAAAAGGTATTGAGGAGAGCATTAA
AGCAAAATGTCTTTCAAATATTCAATTGATTAGACCAAAAAATCGTAAAGATACGCTTGCTGAAATTGCTGCTGCGGATA
TAGCTTATGTTAGTCTTGTAGAAAACGAAGTATTCAAAAAGGTTCTCCCAGGGAAAGTTATGGATTACATGGGAATGAGA
AAACCGATTGTTGCAGATGTTGATGGCTATGCAAAAAAAGTTATTGAAGAGGCACAGTGCGGTTTTGTTGCTAAAAACAG
AACGGTTATTGAACTAGGAAATTATATTATCAAATTGGGTCAAAATAGTCAGTTGCGAGATCAACTAGGTAAAAATGGAT
ATCAATATGCATTTCAAACATTACGTTGGAAAACAAATATTGAAACTTTAGTACGGATACTGGAGGAAAGAGATGTCACA
GAAGAAAGTTTGCATGTTTGTATGGAATCACTTCACAAATGA

Upstream 100 bases:

>100_bases
TTACTTTTTATATATTTTTTTTAGATTTAATATTGGGGTATAAATAATAGAGATTATTAATATGATGTTAACATCATATT
TGGGCGGAGATTAGTTTAGT

Downstream 100 bases:

>100_bases
TGCACGTGTATTAAGAGAATGTACGGCTTTAGGGGAATCTGGATACGAAGTGGATTTAATTTGTATTCATGATTGGAAGC
AAGAAGATCTTCCTATGTGG

Product: glycosyl transferase, group 1; capsular polysaccharide biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKKILIVTQNFYPEIGSAANRMKSVYLELKERGYDVTVLTSDPSYPNRNLYKDPNYWYDEDIEKDTERIHPKTRKYTRNL
FNRLILYIEVALRLILAICKDKKKYDYVFVSTPSIFIAVAGMFAKRKMKTKLILDVRDLWPESLVGIGFFDRKWILKFAY
KLERKIYHSADKIIINSHGFLPYITSKGIPMNRISFMPNSLTEEELSIVQKENSSNQLRVIYTGNIGLAQDIKKLISIAE
YLKDYENISFKIIGYGYQRKGIEESIKAKCLSNIQLIRPKNRKDTLAEIAAADIAYVSLVENEVFKKVLPGKVMDYMGMR
KPIVADVDGYAKKVIEEAQCGFVAKNRTVIELGNYIIKLGQNSQLRDQLGKNGYQYAFQTLRWKTNIETLVRILEERDVT
EESLHVCMESLHK

Sequences:

>Translated_413_residues
MKKILIVTQNFYPEIGSAANRMKSVYLELKERGYDVTVLTSDPSYPNRNLYKDPNYWYDEDIEKDTERIHPKTRKYTRNL
FNRLILYIEVALRLILAICKDKKKYDYVFVSTPSIFIAVAGMFAKRKMKTKLILDVRDLWPESLVGIGFFDRKWILKFAY
KLERKIYHSADKIIINSHGFLPYITSKGIPMNRISFMPNSLTEEELSIVQKENSSNQLRVIYTGNIGLAQDIKKLISIAE
YLKDYENISFKIIGYGYQRKGIEESIKAKCLSNIQLIRPKNRKDTLAEIAAADIAYVSLVENEVFKKVLPGKVMDYMGMR
KPIVADVDGYAKKVIEEAQCGFVAKNRTVIELGNYIIKLGQNSQLRDQLGKNGYQYAFQTLRWKTNIETLVRILEERDVT
EESLHVCMESLHK
>Mature_413_residues
MKKILIVTQNFYPEIGSAANRMKSVYLELKERGYDVTVLTSDPSYPNRNLYKDPNYWYDEDIEKDTERIHPKTRKYTRNL
FNRLILYIEVALRLILAICKDKKKYDYVFVSTPSIFIAVAGMFAKRKMKTKLILDVRDLWPESLVGIGFFDRKWILKFAY
KLERKIYHSADKIIINSHGFLPYITSKGIPMNRISFMPNSLTEEELSIVQKENSSNQLRVIYTGNIGLAQDIKKLISIAE
YLKDYENISFKIIGYGYQRKGIEESIKAKCLSNIQLIRPKNRKDTLAEIAAADIAYVSLVENEVFKKVLPGKVMDYMGMR
KPIVADVDGYAKKVIEEAQCGFVAKNRTVIELGNYIIKLGQNSQLRDQLGKNGYQYAFQTLRWKTNIETLVRILEERDVT
EESLHVCMESLHK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=345, Percent_Identity=20.2898550724638, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 47960; Mature: 47960

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILIVTQNFYPEIGSAANRMKSVYLELKERGYDVTVLTSDPSYPNRNLYKDPNYWYDE
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCC
DIEKDTERIHPKTRKYTRNLFNRLILYIEVALRLILAICKDKKKYDYVFVSTPSIFIAVA
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHH
GMFAKRKMKTKLILDVRDLWPESLVGIGFFDRKWILKFAYKLERKIYHSADKIIINSHGF
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
LPYITSKGIPMNRISFMPNSLTEEELSIVQKENSSNQLRVIYTGNIGLAQDIKKLISIAE
CEEECCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
YLKDYENISFKIIGYGYQRKGIEESIKAKCLSNIQLIRPKNRKDTLAEIAAADIAYVSLV
HHHHHCCCEEEEEEECHHHCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHH
ENEVFKKVLPGKVMDYMGMRKPIVADVDGYAKKVIEEAQCGFVAKNRTVIELGNYIIKLG
HHHHHHHHCCHHHHHHHCCCCCCEECCCHHHHHHHHHHHCCEEECCCCCEEECCCCEEEC
QNSQLRDQLGKNGYQYAFQTLRWKTNIETLVRILEERDVTEESLHVCMESLHK
CCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKILIVTQNFYPEIGSAANRMKSVYLELKERGYDVTVLTSDPSYPNRNLYKDPNYWYDE
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCC
DIEKDTERIHPKTRKYTRNLFNRLILYIEVALRLILAICKDKKKYDYVFVSTPSIFIAVA
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHH
GMFAKRKMKTKLILDVRDLWPESLVGIGFFDRKWILKFAYKLERKIYHSADKIIINSHGF
HHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
LPYITSKGIPMNRISFMPNSLTEEELSIVQKENSSNQLRVIYTGNIGLAQDIKKLISIAE
CEEECCCCCCCHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
YLKDYENISFKIIGYGYQRKGIEESIKAKCLSNIQLIRPKNRKDTLAEIAAADIAYVSLV
HHHHHCCCEEEEEEECHHHCCHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHH
ENEVFKKVLPGKVMDYMGMRKPIVADVDGYAKKVIEEAQCGFVAKNRTVIELGNYIIKLG
HHHHHHHHCCHHHHHHHCCCCCCEECCCHHHHHHHHHHHCCEEECCCCCEEECCCCEEEC
QNSQLRDQLGKNGYQYAFQTLRWKTNIETLVRILEERDVTEESLHVCMESLHK
CCCHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]