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The map label for this gene is nuoJ [H]

Identifier: 52140267

GI number: 52140267

Start: 5097018

End: 5097542

Strand: Reverse

Name: nuoJ [H]

Synonym: BCZK4994

Alternate gene names: 52140267

Gene position: 5097542-5097018 (Counterclockwise)

Preceding gene: 52140266

Following gene: 52140268

Centisome position: 96.16

GC content: 36.38

Gene sequence:

>525_bases
ATGAATGGCGAGTTTATAGCATTCTTTATACTATCCTTGTCTGCAATTATCGGTGGCGTTCTTATGTTGAATTTAACGAA
AGTCATGCATATGATGCTAGCTCTCGTTCTGACATTTCTCAGCATTGCGGGTTTGTACTTTCTTTTATCAGCTGAATTTA
TAGGGGTTGCACAAATTTTACTTTACTCGGGTGCGATCACAATTATTATGATTTTTGGCATTATGTTAACGAAACATAAC
GCGGAAAATGAATCACGTCTTACTCTTCGAAAATGGATTATCTTCTTTGCGGTTGTAGCATTTGGGGCAGTTATGTATTT
CGCTGTTAACAATATTGATTTTGCAAATGAGAGCGCACAAGGAAGTTTACCTCTCCATGAAAAAAACACACTTCAAATCG
GTACACTTCTCTATTCAAAATATATTATTCCATTTGAGTTAACCTCAGTTATTTTACTTGTAGCACTTGTTGGCGCAATT
ATTCTTGCGAAGAAGGATGAGAAAGAGGAGGATTCCAATGAGTAG

Upstream 100 bases:

>100_bases
CAAATAACTTTGAACTTGCGGAGTATTCACGGGATGACTTATTTAAAAATTTGCAGTGGCTTGATGAAAACGACGAAAAC
GTCAGGAAGGAGAATAAAGC

Downstream 100 bases:

>100_bases
CGTACCTGCTTCTGCATATTTAACGCTTGCGATTATTTTGTTTTGCATCGGTTTGTTTGGAGCTTTAACGAAGCGAAATA
CAGTGATTGTATTAGTTTGT

Product: NADH dehydrogenase subunit J

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 6; NDH-1, chain 6 [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MNGEFIAFFILSLSAIIGGVLMLNLTKVMHMMLALVLTFLSIAGLYFLLSAEFIGVAQILLYSGAITIIMIFGIMLTKHN
AENESRLTLRKWIIFFAVVAFGAVMYFAVNNIDFANESAQGSLPLHEKNTLQIGTLLYSKYIIPFELTSVILLVALVGAI
ILAKKDEKEEDSNE

Sequences:

>Translated_174_residues
MNGEFIAFFILSLSAIIGGVLMLNLTKVMHMMLALVLTFLSIAGLYFLLSAEFIGVAQILLYSGAITIIMIFGIMLTKHN
AENESRLTLRKWIIFFAVVAFGAVMYFAVNNIDFANESAQGSLPLHEKNTLQIGTLLYSKYIIPFELTSVILLVALVGAI
ILAKKDEKEEDSNE
>Mature_174_residues
MNGEFIAFFILSLSAIIGGVLMLNLTKVMHMMLALVLTFLSIAGLYFLLSAEFIGVAQILLYSGAITIIMIFGIMLTKHN
AENESRLTLRKWIIFFAVVAFGAVMYFAVNNIDFANESAQGSLPLHEKNTLQIGTLLYSKYIIPFELTSVILLVALVGAI
ILAKKDEKEEDSNE

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG0839

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 6 (chain J)

Gene ontology:

Cell location: Membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 6 family [H]

Homologues:

Organism=Escherichia coli, GI1788616, Length=169, Percent_Identity=28.9940828402367, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001457 [H]

Pfam domain/function: PF00499 Oxidored_q3 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 19228; Mature: 19228

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGEFIAFFILSLSAIIGGVLMLNLTKVMHMMLALVLTFLSIAGLYFLLSAEFIGVAQIL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYSGAITIIMIFGIMLTKHNAENESRLTLRKWIIFFAVVAFGAVMYFAVNNIDFANESAQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GSLPLHEKNTLQIGTLLYSKYIIPFELTSVILLVALVGAIILAKKDEKEEDSNE
CCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNGEFIAFFILSLSAIIGGVLMLNLTKVMHMMLALVLTFLSIAGLYFLLSAEFIGVAQIL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYSGAITIIMIFGIMLTKHNAENESRLTLRKWIIFFAVVAFGAVMYFAVNNIDFANESAQ
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GSLPLHEKNTLQIGTLLYSKYIIPFELTSVILLVALVGAIILAKKDEKEEDSNE
CCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1463844; 8590279; 8905231 [H]