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The map label for this gene is glyA [H]

Identifier: 52140246

GI number: 52140246

Start: 5115700

End: 5116944

Strand: Reverse

Name: glyA [H]

Synonym: BCZK5015

Alternate gene names: 52140246

Gene position: 5116944-5115700 (Counterclockwise)

Preceding gene: 52140244

Following gene: 52140248

Centisome position: 96.53

GC content: 39.76

Gene sequence:

>1245_bases
TTGGTGGATCATTTAAAACGTCAAGATGAAAAGGTATTTGCTGCAATTGAGGCAGAACTAGGAAGACAGCGTTCGAAGAT
TGAGTTAATTGCTTCGGAAAACTTCGTAAGTGAAGCGGTAATGGAGGCGCAAGGTTCTGTTTTAACGAATAAGTATGCTG
AAGGATATCCTGGGAAACGTTACTATGGTGGTTGTGAACACGTAGACGTAGTAGAAGATATCGCACGTGATCGTGTAAAA
GAAATTTTCGGCGCAGAGCATGTAAATGTTCAACCTCATTCTGGTGCACAAGCGAACATGGCAGTATACTTCACGATTTT
AGAGCAAGGCGATACAGTACTTGGTATGAATTTATCTCATGGTGGTCACTTAACACACGGAAGCCCTGTTAACTTCAGTG
GAGTACAATATAATTTCGTAGAATATGGCGTGGATGCTGAATCTCACCGTATTAATTACGATGATGTATTAGCAAAAGCG
AAAGAACATAAACCAAAATTAATCGTTGCAGGTGCAAGTGCATACCCTCGTGTTATCGATTTCAAACGATTCCGTGAGAT
TGCAGATGAAGTGGGCGCATACTTAATGGTTGATATGGCACATATCGCTGGTCTAGTAGCTGCTGGTTTACATCCAAACC
CAGTACCACATGCACATTTCGTTACAACGACAACACATAAAACGTTACGTGGCCCACGTGGTGGTATGATTTTATGTGAA
GAACAATTTGCAAAACAAATTGATAAATCAATCTTCCCTGGTATTCAAGGTGGTCCACTGATGCACGTAATTGCTGCAAA
AGCTGTTGCGTTTGGTGAGGCACTTCAAGATGATTTCAAAACATATGCACAAAATATCATTAACAATGCGAACCGCTTAG
CTGAAGGTCTTCAAAAAGAAGGACTTACACTTGTTTCTGGTGGAACAGACAATCACTTAATCTTAATTGATGTTCGTAAC
TTAGAAATCACAGGTAAAGTAGCAGAGCATGTATTAGATGAAGTTGGTATTACAGTGAACAAAAATACAATTCCATTTGA
AACAGCAAGCCCATTTGTAACAAGTGGTGTACGTATCGGTACGGCAGCTGTAACATCTCGTGGTTTCGGTTTAGAAGAAA
TGGATGAAATTGCGTCACTTATTGCTTACACATTAAAAAATCATGAAAATGAAGCTGCATTAGAAGAAGCACGTAAGCGT
GTAGAAGCGTTAACGAGCAAATTCCCAATGTACACAGATCTATAA

Upstream 100 bases:

>100_bases
ATGAAGGTTCGAAAGAATTACAACCTGAATTTTCGTTTTTTCTGAATAAATAAGAAAAAACTGTTGGGAATCGATGTAAT
TCGTTCAGAAAAGGGGGATT

Downstream 100 bases:

>100_bases
TAGATTGAAGAAGACTGCTGAAACGTAATTGTTTTGGCAGTCTTTTTTGTGGACATATATTGTTCTTTAAGTATGTATAC
AAATGATGAATAAATTTTGG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCEHVDVVEDIARDRVK
EIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVEYGVDAESHRINYDDVLAKA
KEHKPKLIVAGASAYPRVIDFKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKEGLTLVSGGTDNHLILIDVRN
LEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFGLEEMDEIASLIAYTLKNHENEAALEEARKR
VEALTSKFPMYTDL

Sequences:

>Translated_414_residues
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCEHVDVVEDIARDRVK
EIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVEYGVDAESHRINYDDVLAKA
KEHKPKLIVAGASAYPRVIDFKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKEGLTLVSGGTDNHLILIDVRN
LEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFGLEEMDEIASLIAYTLKNHENEAALEEARKR
VEALTSKFPMYTDL
>Mature_414_residues
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCEHVDVVEDIARDRVK
EIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVEYGVDAESHRINYDDVLAKA
KEHKPKLIVAGASAYPRVIDFKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKEGLTLVSGGTDNHLILIDVRN
LEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFGLEEMDEIASLIAYTLKNHENEAALEEARKR
VEALTSKFPMYTDL

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=453, Percent_Identity=41.7218543046358, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI261862352, Length=453, Percent_Identity=41.7218543046358, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI261862350, Length=453, Percent_Identity=41.7218543046358, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI261862348, Length=453, Percent_Identity=41.7218543046358, Blast_Score=342, Evalue=4e-94,
Organism=Homo sapiens, GI261862346, Length=448, Percent_Identity=41.7410714285714, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI22547186, Length=457, Percent_Identity=41.5754923413567, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI22547189, Length=438, Percent_Identity=41.0958904109589, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=58.0335731414868, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI25144732, Length=453, Percent_Identity=42.3841059602649, Blast_Score=354, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI25144729, Length=453, Percent_Identity=42.3841059602649, Blast_Score=354, Evalue=5e-98,
Organism=Saccharomyces cerevisiae, GI6319739, Length=422, Percent_Identity=45.9715639810426, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323087, Length=405, Percent_Identity=43.4567901234568, Blast_Score=319, Evalue=5e-88,
Organism=Drosophila melanogaster, GI24640005, Length=452, Percent_Identity=41.8141592920354, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=452, Percent_Identity=41.8141592920354, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45278; Mature: 45278

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKR
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCC
YYGGCEHVDVVEDIARDRVKEIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSH
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECC
GGHLTHGSPVNFSGVQYNFVEYGVDAESHRINYDDVLAKAKEHKPKLIVAGASAYPRVID
CCCCCCCCCCCCCCEEEEHEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHH
FKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHCCCCCCEEEEH
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTLVSGGTDNHLILIDVRNLEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIG
CCEEEECCCCCEEEEEEECCEEECHHHHHHHHHHCCCEECCCCCCCCCCCCHHHCCEEEC
TAAVTSRGFGLEEMDEIASLIAYTLKNHENEAALEEARKRVEALTSKFPMYTDL
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKR
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCC
YYGGCEHVDVVEDIARDRVKEIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSH
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECC
GGHLTHGSPVNFSGVQYNFVEYGVDAESHRINYDDVLAKAKEHKPKLIVAGASAYPRVID
CCCCCCCCCCCCCCEEEEHEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHH
FKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHCCCCCCEEEEH
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTLVSGGTDNHLILIDVRNLEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIG
CCEEEECCCCCEEEEEEECCEEECHHHHHHHHHHCCCEECCCCCCCCCCCCHHHCCEEEC
TAAVTSRGFGLEEMDEIASLIAYTLKNHENEAALEEARKRVEALTSKFPMYTDL
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA