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The map label for this gene is wcaJ [H]

Identifier: 51893854

GI number: 51893854

Start: 2914055

End: 2915431

Strand: Reverse

Name: wcaJ [H]

Synonym: STH2716

Alternate gene names: 51893854

Gene position: 2915431-2914055 (Counterclockwise)

Preceding gene: 51893855

Following gene: 51893853

Centisome position: 81.75

GC content: 55.56

Gene sequence:

>1377_bases
GTGCCTGGACAAGGTGGTTCATGGCTCTGGGGTGCCCTTGTAGACATCATGCTGCTTGTCGCAGCTCTCGCAGGAGCTAT
GGTCTGGCGAGTTGGGTGGCCTCCACCTGAGACGAACCTGCAGTCCCTGTTAGTCGTCGCACCTATAACGTTCATGGCTT
TACCATTTGTTTTTTTCGGGTTCGGTCTATATCTTAGGAAGCCTGCTCGATCCATTGTGCGGTCAGCGCTATTCGCAGAG
AGTGCGTTGTTTCTCGTGGCACTTGCGGCTGCGTTCTGGTTTAGGGGTTTCTCGTCCCCTAGGTCGGTCTTGGTTGTAGG
ATTCTTGATTCATTTTATCGCGGTGATCGGGTGGCGGTGGGGACTGCAGAAAACCAAGCATGCCCGGGCGTCGCGGGTCA
CGGTGGTTGCGACTAGGGACGAAGCCGTAGCGGTTGTTGAGAAGCTACTATATGAGCCTTCGGGCTGGTATGAAATCGTC
CGTGTCATAGAGCCGGGCCAACTCCTTGCAGAAGGGGATGTTGCCCTGGTTGGGTCTGATATGGTGGTGGCTGGGCCGTC
TATTGTGGGGAGAACGAGGCTTGTGTTGTTGGAGACGGCTGTGCGCGCTGGGGCCAGGGTATTCATTATGCCCGAGCTGA
GTGACATTCTCATGGTAGGGTCGGTTGCCGGCCAGATCGGAGATGCTCCGGTGTTCGAGATACGGCCTCTTATGTTAACC
GGTCGTCAACTGGTCGGAAAGCGCGTCCTTGACCTTTCCCTGTCCATCCCCTTGCTCATCATGTTACTGCCCGTGATGGC
AGCGGTGGCTCTTGCGATAAAGCTTTTCTCTCCCGGGCCGGTCTTCTTCACCCAGAAGCGCGTCGGGCTCAATGGCAAGG
AGTTTGTCTTGTACAAGTTCCGTACCATGGTGGTTGACGCCGAAAAGCGAACGGGACCAGTTCTGGCTACGGCCAACGAT
CCTCGGATCACTCCGATCGGGGAATTCTTGCGGGCAACTCGTTTGGATGAGCTACCTCAACTGATCAACGTCGTGCGGGG
CGAGATGAGTTTGGTAGGTCCGCGACCAGAACGTCCCTATTTCGTAGATCAATTTTGCCGTGAACTGCCAGATTATGATC
TGCGCCACCTGACTCCTCCCGGGATTACGGGGCTAGCGCAAGTAATGGGCAGGTACTATACGAGCGCGCAGGACAAGCTG
CGATTCGATCTGTATTATATACGCCACTATTCGATTTGGCTCGACCTTAAGATCCTGTTGTTGACGCTACAAGCCGTCAT
TTCTAGGGAAAGTGCAACTGGAGTTGAGGCTAGCCCAACGCCACAGCGGATGAGAGCCGAGCGACTGGTGTTGGACCGTT
CGGGGCGTGTACCGTGA

Upstream 100 bases:

>100_bases
CGGATATTACGTTGATGGCAGGAAGTGGCTGGACAACTGGGTGGTTCACAGTCAGTGGCTCTTAGGCAAGTTTCTTCTTT
GTTGAACGGGAGTGACGGCT

Downstream 100 bases:

>100_bases
TTGTCAAGTATGGTCTCGGTATGGGGTGTGTGATGAGGACTGTTCTTATTACAGGCGGAGCTGGGTTCGTTGGTTCACAT
GTAGTTGAGCGGTTTCTAGC

Product: lipopolysaccharide synthesis sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MPGQGGSWLWGALVDIMLLVAALAGAMVWRVGWPPPETNLQSLLVVAPITFMALPFVFFGFGLYLRKPARSIVRSALFAE
SALFLVALAAAFWFRGFSSPRSVLVVGFLIHFIAVIGWRWGLQKTKHARASRVTVVATRDEAVAVVEKLLYEPSGWYEIV
RVIEPGQLLAEGDVALVGSDMVVAGPSIVGRTRLVLLETAVRAGARVFIMPELSDILMVGSVAGQIGDAPVFEIRPLMLT
GRQLVGKRVLDLSLSIPLLIMLLPVMAAVALAIKLFSPGPVFFTQKRVGLNGKEFVLYKFRTMVVDAEKRTGPVLATAND
PRITPIGEFLRATRLDELPQLINVVRGEMSLVGPRPERPYFVDQFCRELPDYDLRHLTPPGITGLAQVMGRYYTSAQDKL
RFDLYYIRHYSIWLDLKILLLTLQAVISRESATGVEASPTPQRMRAERLVLDRSGRVP

Sequences:

>Translated_458_residues
MPGQGGSWLWGALVDIMLLVAALAGAMVWRVGWPPPETNLQSLLVVAPITFMALPFVFFGFGLYLRKPARSIVRSALFAE
SALFLVALAAAFWFRGFSSPRSVLVVGFLIHFIAVIGWRWGLQKTKHARASRVTVVATRDEAVAVVEKLLYEPSGWYEIV
RVIEPGQLLAEGDVALVGSDMVVAGPSIVGRTRLVLLETAVRAGARVFIMPELSDILMVGSVAGQIGDAPVFEIRPLMLT
GRQLVGKRVLDLSLSIPLLIMLLPVMAAVALAIKLFSPGPVFFTQKRVGLNGKEFVLYKFRTMVVDAEKRTGPVLATAND
PRITPIGEFLRATRLDELPQLINVVRGEMSLVGPRPERPYFVDQFCRELPDYDLRHLTPPGITGLAQVMGRYYTSAQDKL
RFDLYYIRHYSIWLDLKILLLTLQAVISRESATGVEASPTPQRMRAERLVLDRSGRVP
>Mature_457_residues
PGQGGSWLWGALVDIMLLVAALAGAMVWRVGWPPPETNLQSLLVVAPITFMALPFVFFGFGLYLRKPARSIVRSALFAES
ALFLVALAAAFWFRGFSSPRSVLVVGFLIHFIAVIGWRWGLQKTKHARASRVTVVATRDEAVAVVEKLLYEPSGWYEIVR
VIEPGQLLAEGDVALVGSDMVVAGPSIVGRTRLVLLETAVRAGARVFIMPELSDILMVGSVAGQIGDAPVFEIRPLMLTG
RQLVGKRVLDLSLSIPLLIMLLPVMAAVALAIKLFSPGPVFFTQKRVGLNGKEFVLYKFRTMVVDAEKRTGPVLATANDP
RITPIGEFLRATRLDELPQLINVVRGEMSLVGPRPERPYFVDQFCRELPDYDLRHLTPPGITGLAQVMGRYYTSAQDKLR
FDLYYIRHYSIWLDLKILLLTLQAVISRESATGVEASPTPQRMRAERLVLDRSGRVP

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=224, Percent_Identity=41.9642857142857, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 50647; Mature: 50515

Theoretical pI: Translated: 10.31; Mature: 10.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGQGGSWLWGALVDIMLLVAALAGAMVWRVGWPPPETNLQSLLVVAPITFMALPFVFFG
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FGLYLRKPARSIVRSALFAESALFLVALAAAFWFRGFSSPRSVLVVGFLIHFIAVIGWRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GLQKTKHARASRVTVVATRDEAVAVVEKLLYEPSGWYEIVRVIEPGQLLAEGDVALVGSD
CCHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEECCCEEEEECC
MVVAGPSIVGRTRLVLLETAVRAGARVFIMPELSDILMVGSVAGQIGDAPVFEIRPLMLT
EEEECCCHHCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCHHEECHHHHH
GRQLVGKRVLDLSLSIPLLIMLLPVMAAVALAIKLFSPGPVFFTQKRVGLNGKEFVLYKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEE
RTMVVDAEKRTGPVLATANDPRITPIGEFLRATRLDELPQLINVVRGEMSLVGPRPERPY
EEHEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCC
FVDQFCRELPDYDLRHLTPPGITGLAQVMGRYYTSAQDKLRFDLYYIRHYSIWLDLKILL
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEEEEEEEHHHHHHHHHHHH
LTLQAVISRESATGVEASPTPQRMRAERLVLDRSGRVP
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PGQGGSWLWGALVDIMLLVAALAGAMVWRVGWPPPETNLQSLLVVAPITFMALPFVFFG
CCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FGLYLRKPARSIVRSALFAESALFLVALAAAFWFRGFSSPRSVLVVGFLIHFIAVIGWRW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GLQKTKHARASRVTVVATRDEAVAVVEKLLYEPSGWYEIVRVIEPGQLLAEGDVALVGSD
CCHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEECCCEEEEECC
MVVAGPSIVGRTRLVLLETAVRAGARVFIMPELSDILMVGSVAGQIGDAPVFEIRPLMLT
EEEECCCHHCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCCHHEECHHHHH
GRQLVGKRVLDLSLSIPLLIMLLPVMAAVALAIKLFSPGPVFFTQKRVGLNGKEFVLYKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEE
RTMVVDAEKRTGPVLATANDPRITPIGEFLRATRLDELPQLINVVRGEMSLVGPRPERPY
EEHEEECCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCC
FVDQFCRELPDYDLRHLTPPGITGLAQVMGRYYTSAQDKLRFDLYYIRHYSIWLDLKILL
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEEEEEEEHHHHHHHHHHHH
LTLQAVISRESATGVEASPTPQRMRAERLVLDRSGRVP
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]