Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 51893825

GI number: 51893825

Start: 2876778

End: 2878349

Strand: Reverse

Name: leuA [H]

Synonym: STH2687

Alternate gene names: 51893825

Gene position: 2878349-2876778 (Counterclockwise)

Preceding gene: 161899037

Following gene: 51893824

Centisome position: 80.71

GC content: 69.85

Gene sequence:

>1572_bases
GTGACGCGGCGGATTCGGATCTTCGACACCACCCTGCGGGATGGTGAGCAGGCTCCTGGGTTCGGCATGACGGCGGAGGC
CAAGCTGGAGGTGGCCCGCCAGCTTAGCAAGCTGGGCGTCGACGTCATTGAGGCCGGTTTTCCCGCCGCCTCGCCCGGCG
ACTTCGAGGCCGTGCGGACGATCGCCGAGACCATCACCGGGCCCACCATCGCCGGTCTGGCCCGGGCCAACGAGGACGAC
ATCCGGCGCTGTTACGAGGCGGTGAAGGGCGCGCAGAAGCCCCGCATCCACACCTTCATCGCCACCTCGCCCATTCACAT
GGAGTACAAGCTGCGCAAGAAGCCGGAAGAGGTGGTGCGGGCGGCCCGGGAAGCCGTCGCCCTGGCCCGGTCGCTGGGGC
CCGAGGTCGAGTTCTCCGCTGAAGACGCGACCCGGTCCGACTGGAACTTCCTGGTCCAGATCTTCACCGTGGCTGCCCGG
GCGGGCGCGACGATCCTCAACGTGCCCGACACCGTGGGGTACACCACGCCGAAGGAGTACTACGACCTGATCCGGTACCT
CATCGAGCACGTGGACGCCCCCGAGGGGGTCCAGTTCTCCGTCCACTGCCACAACGACCTGGGCCTGGCGGTGGCCAACA
CCCTGGCCGCCATCGAGGCCGGCGCCACGCAGGTGGAGTGCACCATCAACGGCATCGGCGAGCGGGCGGGCAACGCGGCC
CTGGAGGAGATCGTGATGGCCCTGCGGGTCCGGCGGGACCAGTGGGGCGCCACGACCGCCATCAACACCCGCGAGATCTA
CCGCACCTCCCGGCTGCTGCAGAGCGTCACGGGCATCCAGGTGCAGCCCAACAAGGCCATCGTCGGCGCCAACGCCTTCG
CCCACGAGTCCGGCATCCACCAGGACGGCATGCTGAAGCACCGGGACACCTACGAGATCATGAAGCCCGAAGACGTGGGC
GTCCCCGAGTCGTCCCTGGTGCTGGGCAAGCACTCCGGGCGGGCGGCCTTCCGGGCCCGGCTGGAGCACCTGGGTTACCA
GCTCACCGACGCCGAGATCGACTCCGCCTTCCGGCGGTTCAAGGAGCTGGCCGACCGCAAGCGGACGATCACGGACCACG
ACCTGATGGCGATCGTCGGCGACGAGCAGGCGCACCGGACGGTGACCGAGGTCTGCCGGCTCGAGTCCTTCCAGGTGGTC
GCGGGCAACGAGCGCATTGCGACCGCGTCGGTCCGGCTGGTGCGGGAAGGCGAGGTGGTCCAGGAGGCGGCCTCGGGCGA
CGGGCCCATCAGCGCCCTCTACCACGCCATTGACCGGGCGGCCCGCTTCGAGGGGCAGCTGCTGGATTACCGGCTGAACG
CCGTGACGGAAGGCGAGGATGCGCTGGGCGAGGTGACGGTGCGGGTGCGCGCGGGCGACCGGGTCGCCTCGGGGCGGGGC
ACCTCCACGGACGTCATCGAGGCGTCTGCCAGGGCCTACATGAACGCGATGAACAAGATCCTCTCCGGGGCCGCGCTGGC
CGTGTCGGACGATCCGGCGCCGGGCTGGCAGATGCGGGCCTACGGGGACTGA

Upstream 100 bases:

>100_bases
AGGCGCGGACCCACACGAATGCGAACAGCACGGATGCGCTGGGGACACACCCCCTTTTGGGGGCCCCGATAATTCACGAG
CGAAAAGGATGGTGACGGCG

Downstream 100 bases:

>100_bases
GGGGGGGCGTTGGCGGTGTCCATCCAGGTCTACGTGGACGGCGAGTGGTACTCCCGGGACGAGGCCAAGGTGTCCGTGTT
TGACCACGGCTTCCTGTACG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MTRRIRIFDTTLRDGEQAPGFGMTAEAKLEVARQLSKLGVDVIEAGFPAASPGDFEAVRTIAETITGPTIAGLARANEDD
IRRCYEAVKGAQKPRIHTFIATSPIHMEYKLRKKPEEVVRAAREAVALARSLGPEVEFSAEDATRSDWNFLVQIFTVAAR
AGATILNVPDTVGYTTPKEYYDLIRYLIEHVDAPEGVQFSVHCHNDLGLAVANTLAAIEAGATQVECTINGIGERAGNAA
LEEIVMALRVRRDQWGATTAINTREIYRTSRLLQSVTGIQVQPNKAIVGANAFAHESGIHQDGMLKHRDTYEIMKPEDVG
VPESSLVLGKHSGRAAFRARLEHLGYQLTDAEIDSAFRRFKELADRKRTITDHDLMAIVGDEQAHRTVTEVCRLESFQVV
AGNERIATASVRLVREGEVVQEAASGDGPISALYHAIDRAARFEGQLLDYRLNAVTEGEDALGEVTVRVRAGDRVASGRG
TSTDVIEASARAYMNAMNKILSGAALAVSDDPAPGWQMRAYGD

Sequences:

>Translated_523_residues
MTRRIRIFDTTLRDGEQAPGFGMTAEAKLEVARQLSKLGVDVIEAGFPAASPGDFEAVRTIAETITGPTIAGLARANEDD
IRRCYEAVKGAQKPRIHTFIATSPIHMEYKLRKKPEEVVRAAREAVALARSLGPEVEFSAEDATRSDWNFLVQIFTVAAR
AGATILNVPDTVGYTTPKEYYDLIRYLIEHVDAPEGVQFSVHCHNDLGLAVANTLAAIEAGATQVECTINGIGERAGNAA
LEEIVMALRVRRDQWGATTAINTREIYRTSRLLQSVTGIQVQPNKAIVGANAFAHESGIHQDGMLKHRDTYEIMKPEDVG
VPESSLVLGKHSGRAAFRARLEHLGYQLTDAEIDSAFRRFKELADRKRTITDHDLMAIVGDEQAHRTVTEVCRLESFQVV
AGNERIATASVRLVREGEVVQEAASGDGPISALYHAIDRAARFEGQLLDYRLNAVTEGEDALGEVTVRVRAGDRVASGRG
TSTDVIEASARAYMNAMNKILSGAALAVSDDPAPGWQMRAYGD
>Mature_522_residues
TRRIRIFDTTLRDGEQAPGFGMTAEAKLEVARQLSKLGVDVIEAGFPAASPGDFEAVRTIAETITGPTIAGLARANEDDI
RRCYEAVKGAQKPRIHTFIATSPIHMEYKLRKKPEEVVRAAREAVALARSLGPEVEFSAEDATRSDWNFLVQIFTVAARA
GATILNVPDTVGYTTPKEYYDLIRYLIEHVDAPEGVQFSVHCHNDLGLAVANTLAAIEAGATQVECTINGIGERAGNAAL
EEIVMALRVRRDQWGATTAINTREIYRTSRLLQSVTGIQVQPNKAIVGANAFAHESGIHQDGMLKHRDTYEIMKPEDVGV
PESSLVLGKHSGRAAFRARLEHLGYQLTDAEIDSAFRRFKELADRKRTITDHDLMAIVGDEQAHRTVTEVCRLESFQVVA
GNERIATASVRLVREGEVVQEAASGDGPISALYHAIDRAARFEGQLLDYRLNAVTEGEDALGEVTVRVRAGDRVASGRGT
STDVIEASARAYMNAMNKILSGAALAVSDDPAPGWQMRAYGD

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=506, Percent_Identity=46.4426877470356, Blast_Score=449, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320071, Length=383, Percent_Identity=30.2872062663185, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6320019, Length=390, Percent_Identity=29.7435897435897, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6324225, Length=565, Percent_Identity=27.9646017699115, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6324682, Length=553, Percent_Identity=27.124773960217, Blast_Score=142, Evalue=9e-35,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56995; Mature: 56864

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRIRIFDTTLRDGEQAPGFGMTAEAKLEVARQLSKLGVDVIEAGFPAASPGDFEAVRT
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHH
IAETITGPTIAGLARANEDDIRRCYEAVKGAQKPRIHTFIATSPIHMEYKLRKKPEEVVR
HHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCHHHHHH
AAREAVALARSLGPEVEFSAEDATRSDWNFLVQIFTVAARAGATILNVPDTVGYTTPKEY
HHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
YDLIRYLIEHVDAPEGVQFSVHCHNDLGLAVANTLAAIEAGATQVECTINGIGERAGNAA
HHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHH
LEEIVMALRVRRDQWGATTAINTREIYRTSRLLQSVTGIQVQPNKAIVGANAFAHESGIH
HHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCEECCCCEEEECCHHHHCCCCC
QDGMLKHRDTYEIMKPEDVGVPESSLVLGKHSGRAAFRARLEHLGYQLTDAEIDSAFRRF
CCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEECHHHHHHHHHHH
KELADRKRTITDHDLMAIVGDEQAHRTVTEVCRLESFQVVAGNERIATASVRLVREGEVV
HHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCEEEEHHHEEECCCHHH
QEAASGDGPISALYHAIDRAARFEGQLLDYRLNAVTEGEDALGEVTVRVRAGDRVASGRG
HHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEEECCCEECCCCC
TSTDVIEASARAYMNAMNKILSGAALAVSDDPAPGWQMRAYGD
CCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEECCC
>Mature Secondary Structure 
TRRIRIFDTTLRDGEQAPGFGMTAEAKLEVARQLSKLGVDVIEAGFPAASPGDFEAVRT
CCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHH
IAETITGPTIAGLARANEDDIRRCYEAVKGAQKPRIHTFIATSPIHMEYKLRKKPEEVVR
HHHHHCCCHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEECCCCHHHHHH
AAREAVALARSLGPEVEFSAEDATRSDWNFLVQIFTVAARAGATILNVPDTVGYTTPKEY
HHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
YDLIRYLIEHVDAPEGVQFSVHCHNDLGLAVANTLAAIEAGATQVECTINGIGERAGNAA
HHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHH
LEEIVMALRVRRDQWGATTAINTREIYRTSRLLQSVTGIQVQPNKAIVGANAFAHESGIH
HHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHCCEECCCCEEEECCHHHHCCCCC
QDGMLKHRDTYEIMKPEDVGVPESSLVLGKHSGRAAFRARLEHLGYQLTDAEIDSAFRRF
CCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEECHHHHHHHHHHH
KELADRKRTITDHDLMAIVGDEQAHRTVTEVCRLESFQVVAGNERIATASVRLVREGEVV
HHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECCCEEEEHHHEEECCCHHH
QEAASGDGPISALYHAIDRAARFEGQLLDYRLNAVTEGEDALGEVTVRVRAGDRVASGRG
HHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEEEECCCEECCCCC
TSTDVIEASARAYMNAMNKILSGAALAVSDDPAPGWQMRAYGD
CCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA