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The map label for this gene is mdeA [H]

Identifier: 51893819

GI number: 51893819

Start: 2869119

End: 2870426

Strand: Reverse

Name: mdeA [H]

Synonym: STH2681

Alternate gene names: 51893819

Gene position: 2870426-2869119 (Counterclockwise)

Preceding gene: 51893820

Following gene: 51893818

Centisome position: 80.49

GC content: 70.49

Gene sequence:

>1308_bases
ATGCCTGACCGCAGCGACGCGGCCTCCGACCGGTACCGGCCCGCGACCCTGGTGGTTCACGCGGGCTGGCAGCCGGATCC
GCTCACGGGCGCGCAGGCGGTGCCCATCTACCAGACCACCGCCTATGCCTTCCAATCGACGGAGCACGCTCGGCAGCTGT
TCTCCCTGGAGGCCGAGGGCAACATCTACACCCGGATTAACAACCCCACCACGGCTGCATTCGAGGCCCGCGTCGCGGCG
CTGGAGGGCGGGATCGGGGCCGTCGCCTTCGCCTCAGGCCACGCCGCGCTGGCGGCGACCGTGCTGGCCATCTGCAACGC
CGGCGACGAGATCGTCACCTCCTCGAGCCTCTACGGCGGCACGTATAACCTCTTCGCGACCACCCTGCCCAAGTGGGGCA
TCACGGCCCGCTTCGTCGACGCGCATGACCTGGCGGGCTTCCACCGGGCCATCACGCCGCGGACCCGCCTGATCTTCGGC
GAGGTGATCGGCAACCCGCGGCTGGACGTGTTCGACATCGCCGGGGTGGCCGCCATCGCGCACGAGCACGGGCTGCCGCT
GGTCATCGACAACACCTTCGCCACGCCCATGCTGTGCCGGCCCATCGAGCACGGGGCCGACCTGGTCATCCACTCGGCCA
CCAAGTGGCTGGGCGGCCACGGCGTGGCCATGGGCGGCGTGGTGGTGGACTCGGGCAACTTCGACTGGAACTCGCCCCGC
TTCCCCGGCTTCACCGAGCCGGACCCGTCGTATCACGGCGTGCGGTACGCCGTCGATTTCGGCCGCGCCGGATTCATCAC
CAAGGTCCGGGCCCAGATGCTGCGGGACCTGGGCGGCTGCCAGTCGCCCTTCAACGCCTTCCTGCTGCTCCTGGGACTGG
ACACCCTGGCCCTGCGCATGCGGGCGATCAGCGAGAACGCCCTGGAGATCGCACGGTACCTGTCGGAGCACCCGGCGGTG
GCCTGGGTGCGGTACCCCGGCCTGCCCAGCCATCCGGATCACGCCCTCGCGCAGCGCTACCTGACCGGAGGCTTCGGCGG
CATGCTGGTCTTCGGCCTGAAGGGAGGCCTGGAGGCGGGCGCCCGCTTCATCGACTCCGTGCGCCTCTTCTCCCATGTGG
CCAACGTGGGCGACGCCCGGTCGCTGGTCATTCACCCCGCGTCCACGACCCACAGCCAGCTGACCCCGGCCCAGCGGGAG
GCCGCCGGCGTGGGCGATGACCTGATCCGGGTGTCCGTGGGGTTCGAGGACGTGGCGGATCTGATTGCCGATCTGGAGCA
GGCCCTGGAGAAGGCGGCGGGTGCGTGA

Upstream 100 bases:

>100_bases
GCGCTGCGGGCCAATCGGCTCGGGGCCGAGGTGGTGACCCGGGTGGGCGCCCGGCCGGCGCTTTCGCCCGAGATCATGCG
GCAGCTGGAGGTGGAACGGG

Downstream 100 bases:

>100_bases
GCCCCTGACGCCGCCATTTCGGCGCGGTCGGCGCCGGCGTCCAAAATATTGAGGTAGAACCAAGGGGGCGATTGGCGTGG
CCGTGAATGACCCGAAGACG

Product: O-acetylhomoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MPDRSDAASDRYRPATLVVHAGWQPDPLTGAQAVPIYQTTAYAFQSTEHARQLFSLEAEGNIYTRINNPTTAAFEARVAA
LEGGIGAVAFASGHAALAATVLAICNAGDEIVTSSSLYGGTYNLFATTLPKWGITARFVDAHDLAGFHRAITPRTRLIFG
EVIGNPRLDVFDIAGVAAIAHEHGLPLVIDNTFATPMLCRPIEHGADLVIHSATKWLGGHGVAMGGVVVDSGNFDWNSPR
FPGFTEPDPSYHGVRYAVDFGRAGFITKVRAQMLRDLGGCQSPFNAFLLLLGLDTLALRMRAISENALEIARYLSEHPAV
AWVRYPGLPSHPDHALAQRYLTGGFGGMLVFGLKGGLEAGARFIDSVRLFSHVANVGDARSLVIHPASTTHSQLTPAQRE
AAGVGDDLIRVSVGFEDVADLIADLEQALEKAAGA

Sequences:

>Translated_435_residues
MPDRSDAASDRYRPATLVVHAGWQPDPLTGAQAVPIYQTTAYAFQSTEHARQLFSLEAEGNIYTRINNPTTAAFEARVAA
LEGGIGAVAFASGHAALAATVLAICNAGDEIVTSSSLYGGTYNLFATTLPKWGITARFVDAHDLAGFHRAITPRTRLIFG
EVIGNPRLDVFDIAGVAAIAHEHGLPLVIDNTFATPMLCRPIEHGADLVIHSATKWLGGHGVAMGGVVVDSGNFDWNSPR
FPGFTEPDPSYHGVRYAVDFGRAGFITKVRAQMLRDLGGCQSPFNAFLLLLGLDTLALRMRAISENALEIARYLSEHPAV
AWVRYPGLPSHPDHALAQRYLTGGFGGMLVFGLKGGLEAGARFIDSVRLFSHVANVGDARSLVIHPASTTHSQLTPAQRE
AAGVGDDLIRVSVGFEDVADLIADLEQALEKAAGA
>Mature_434_residues
PDRSDAASDRYRPATLVVHAGWQPDPLTGAQAVPIYQTTAYAFQSTEHARQLFSLEAEGNIYTRINNPTTAAFEARVAAL
EGGIGAVAFASGHAALAATVLAICNAGDEIVTSSSLYGGTYNLFATTLPKWGITARFVDAHDLAGFHRAITPRTRLIFGE
VIGNPRLDVFDIAGVAAIAHEHGLPLVIDNTFATPMLCRPIEHGADLVIHSATKWLGGHGVAMGGVVVDSGNFDWNSPRF
PGFTEPDPSYHGVRYAVDFGRAGFITKVRAQMLRDLGGCQSPFNAFLLLLGLDTLALRMRAISENALEIARYLSEHPAVA
WVRYPGLPSHPDHALAQRYLTGGFGGMLVFGLKGGLEAGARFIDSVRLFSHVANVGDARSLVIHPASTTHSQLTPAQREA
AGVGDDLIRVSVGFEDVADLIADLEQALEKAAGA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=438, Percent_Identity=35.8447488584475, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI299473759, Length=438, Percent_Identity=32.648401826484, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI25453487, Length=437, Percent_Identity=31.350114416476, Blast_Score=169, Evalue=6e-42,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=33.5714285714286, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789383, Length=417, Percent_Identity=29.7362110311751, Blast_Score=153, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71980708, Length=413, Percent_Identity=34.1404358353511, Blast_Score=232, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI17560056, Length=419, Percent_Identity=36.5155131264916, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17538055, Length=422, Percent_Identity=35.0710900473934, Blast_Score=217, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=47.3317865429234, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6319307, Length=433, Percent_Identity=34.6420323325635, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321254, Length=444, Percent_Identity=25.9009009009009, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6321170, Length=357, Percent_Identity=26.890756302521, Blast_Score=117, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321904, Length=434, Percent_Identity=24.4239631336406, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922554, Length=436, Percent_Identity=34.6330275229358, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46330; Mature: 46199

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDRSDAASDRYRPATLVVHAGWQPDPLTGAQAVPIYQTTAYAFQSTEHARQLFSLEAEG
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHEEECCC
NIYTRINNPTTAAFEARVAALEGGIGAVAFASGHAALAATVLAICNAGDEIVTSSSLYGG
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCHHEECCCCCCC
TYNLFATTLPKWGITARFVDAHDLAGFHRAITPRTRLIFGEVIGNPRLDVFDIAGVAAIA
EEEHHHHCCCCCCCEEEEECHHHHHHHHHHCCCCHHEEEHHHCCCCCCEEEHHHHHHHHH
HEHGLPLVIDNTFATPMLCRPIEHGADLVIHSATKWLGGHGVAMGGVVVDSGNFDWNSPR
HHCCCCEEECCCCCCCHHHCCHHCCCCEEEECHHHHCCCCCEEECCEEEECCCCCCCCCC
FPGFTEPDPSYHGVRYAVDFGRAGFITKVRAQMLRDLGGCQSPFNAFLLLLGLDTLALRM
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RAISENALEIARYLSEHPAVAWVRYPGLPSHPDHALAQRYLTGGFGGMLVFGLKGGLEAG
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCHHHH
ARFIDSVRLFSHVANVGDARSLVIHPASTTHSQLTPAQREAAGVGDDLIRVSVGFEDVAD
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHCCCCCCEEEEEECHHHHHH
LIADLEQALEKAAGA
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDRSDAASDRYRPATLVVHAGWQPDPLTGAQAVPIYQTTAYAFQSTEHARQLFSLEAEG
CCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHEEECCC
NIYTRINNPTTAAFEARVAALEGGIGAVAFASGHAALAATVLAICNAGDEIVTSSSLYGG
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCHHEECCCCCCC
TYNLFATTLPKWGITARFVDAHDLAGFHRAITPRTRLIFGEVIGNPRLDVFDIAGVAAIA
EEEHHHHCCCCCCCEEEEECHHHHHHHHHHCCCCHHEEEHHHCCCCCCEEEHHHHHHHHH
HEHGLPLVIDNTFATPMLCRPIEHGADLVIHSATKWLGGHGVAMGGVVVDSGNFDWNSPR
HHCCCCEEECCCCCCCHHHCCHHCCCCEEEECHHHHCCCCCEEECCEEEECCCCCCCCCC
FPGFTEPDPSYHGVRYAVDFGRAGFITKVRAQMLRDLGGCQSPFNAFLLLLGLDTLALRM
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RAISENALEIARYLSEHPAVAWVRYPGLPSHPDHALAQRYLTGGFGGMLVFGLKGGLEAG
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCHHHH
ARFIDSVRLFSHVANVGDARSLVIHPASTTHSQLTPAQREAAGVGDDLIRVSVGFEDVAD
HHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHCCCCCCEEEEEECHHHHHH
LIADLEQALEKAAGA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]