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The map label for this gene is miaA [H]

Identifier: 51599072

GI number: 51599072

Start: 868573

End: 869493

Strand: Direct

Name: miaA [H]

Synonym: BG0846

Alternate gene names: 51599072

Gene position: 868573-869493 (Clockwise)

Preceding gene: 51599071

Following gene: 51599074

Centisome position: 96.05

GC content: 27.25

Gene sequence:

>921_bases
TTGAAGGAAGATAGAATAGTTTTTATTTTTGGCCCTACAGCTGTGGGCAAAAGCAATATTTTGTTTCATTTTCCAAAAAA
TAAGGCGGAAATTATTAATGTTGACTCTATTCAAGTATATAAAGAGTTCAATATAGCTTCTTCAAAGCCAAGTAAAAATT
TAATGAAACACATAAAGCACCATTTAGTAGATTTTTTAGATCCCGAAAAAGAGTATACTCTTGGAATTTTTTACGAGCAA
GCTTTAAGAATAGTAAAAGAAATAAGACAGAAAAAAAAAATTCCTATATTTGTAGGGGGCACTGCTTTTTATTTTAAACA
TTTAAAGGATGGATTTCCTTCGACACCTTTGGTTACTTCTGAAATAAGAATTTATGTTAATAATCTTTTATATCTTAAAG
GCAAATCCTATCTTTTAAAAGAATTAAAAAATGTAGATCCCATCAGATTTAATATGTTAAATAAGAATGATATTTATCGT
ATTAAAAGATCGCTTGAAGTTTATTACCAAACAGGAATTCCTATTAGCCAATTTGAAAAAAAACAGAATAGCGAATTTAA
AAATATTCTGATTATAGGTCTTAAAAGATCTTTTGAAGATTTGAAAACTAGAATATCAATAAGAATTAATGAAATGCTCA
ATAGCGGCCTACTTTCCGAGATTAAAGGCTTGTTTAGTAAAGGTTATAATGAAAATACGCCGGCTTTTAAAGGGATAGGC
TATAATGAGTTTTTATTATGGAAAAGTAGACCTTGTTATAGTTTAAATGATATAATAAGTTTGATAAATAAAAATTCGTT
TTTATATGCAAAAAGGCAAATGACCTTTTTTGCCAAAATTTCTAATGTTTTATGGTTTCATCCAGAGGATGATTTAGTTA
GCATTTTGAATTTAATTTTTAACGTTGATAAGGAGATTTAG

Upstream 100 bases:

>100_bases
ATCATAGACAATATTTCTTTAGCAGAACATGCTGTTTTTGATGATAAAATATTAGGACAAGCTTTTAATGACGTTTTGAC
TGGTAAATTTAGCTATTCAA

Downstream 100 bases:

>100_bases
GGAGTGCCTTGCGGAAGAAAAAGAAAGTTAAAAAAAATTTCTACTCATAAAAGGAAGAAAAAAAGAAGGAAAAATAGACA
TAAGAAAAAAAATAAGTAAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKEDRIVFIFGPTAVGKSNILFHFPKNKAEIINVDSIQVYKEFNIASSKPSKNLMKHIKHHLVDFLDPEKEYTLGIFYEQ
ALRIVKEIRQKKKIPIFVGGTAFYFKHLKDGFPSTPLVTSEIRIYVNNLLYLKGKSYLLKELKNVDPIRFNMLNKNDIYR
IKRSLEVYYQTGIPISQFEKKQNSEFKNILIIGLKRSFEDLKTRISIRINEMLNSGLLSEIKGLFSKGYNENTPAFKGIG
YNEFLLWKSRPCYSLNDIISLINKNSFLYAKRQMTFFAKISNVLWFHPEDDLVSILNLIFNVDKEI

Sequences:

>Translated_306_residues
MKEDRIVFIFGPTAVGKSNILFHFPKNKAEIINVDSIQVYKEFNIASSKPSKNLMKHIKHHLVDFLDPEKEYTLGIFYEQ
ALRIVKEIRQKKKIPIFVGGTAFYFKHLKDGFPSTPLVTSEIRIYVNNLLYLKGKSYLLKELKNVDPIRFNMLNKNDIYR
IKRSLEVYYQTGIPISQFEKKQNSEFKNILIIGLKRSFEDLKTRISIRINEMLNSGLLSEIKGLFSKGYNENTPAFKGIG
YNEFLLWKSRPCYSLNDIISLINKNSFLYAKRQMTFFAKISNVLWFHPEDDLVSILNLIFNVDKEI
>Mature_306_residues
MKEDRIVFIFGPTAVGKSNILFHFPKNKAEIINVDSIQVYKEFNIASSKPSKNLMKHIKHHLVDFLDPEKEYTLGIFYEQ
ALRIVKEIRQKKKIPIFVGGTAFYFKHLKDGFPSTPLVTSEIRIYVNNLLYLKGKSYLLKELKNVDPIRFNMLNKNDIYR
IKRSLEVYYQTGIPISQFEKKQNSEFKNILIIGLKRSFEDLKTRISIRINEMLNSGLLSEIKGLFSKGYNENTPAFKGIG
YNEFLLWKSRPCYSLNDIISLINKNSFLYAKRQMTFFAKISNVLWFHPEDDLVSILNLIFNVDKEI

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=265, Percent_Identity=29.811320754717, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1790613, Length=310, Percent_Identity=27.4193548387097, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI25144712, Length=292, Percent_Identity=29.1095890410959, Blast_Score=117, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6324848, Length=290, Percent_Identity=30, Blast_Score=118, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24649830, Length=270, Percent_Identity=27.4074074074074, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 35800; Mature: 35800

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEDRIVFIFGPTAVGKSNILFHFPKNKAEIINVDSIQVYKEFNIASSKPSKNLMKHIKH
CCCCCEEEEECCCCCCCCCEEEEECCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHH
HLVDFLDPEKEYTLGIFYEQALRIVKEIRQKKKIPIFVGGTAFYFKHLKDGFPSTPLVTS
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCEEHH
EIRIYVNNLLYLKGKSYLLKELKNVDPIRFNMLNKNDIYRIKRSLEVYYQTGIPISQFEK
HHHEEEEEEEEECCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHEEEECCCCHHHHHH
KQNSEFKNILIIGLKRSFEDLKTRISIRINEMLNSGLLSEIKGLFSKGYNENTPAFKGIG
HCCCCCCEEEEEEEHHHHHHHHHHHHEEEHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCC
YNEFLLWKSRPCYSLNDIISLINKNSFLYAKRQMTFFAKISNVLWFHPEDDLVSILNLIF
CCEEEEECCCCCCCHHHHHHHHCCCCEEEEHHHHHHHEECCCEEEECCCHHHHHHHHHHH
NVDKEI
HCCCCC
>Mature Secondary Structure
MKEDRIVFIFGPTAVGKSNILFHFPKNKAEIINVDSIQVYKEFNIASSKPSKNLMKHIKH
CCCCCEEEEECCCCCCCCCEEEEECCCCCEEEECCCEEEEEECCCCCCCCHHHHHHHHHH
HLVDFLDPEKEYTLGIFYEQALRIVKEIRQKKKIPIFVGGTAFYFKHLKDGFPSTPLVTS
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCEEHH
EIRIYVNNLLYLKGKSYLLKELKNVDPIRFNMLNKNDIYRIKRSLEVYYQTGIPISQFEK
HHHEEEEEEEEECCCHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHEEEECCCCHHHHHH
KQNSEFKNILIIGLKRSFEDLKTRISIRINEMLNSGLLSEIKGLFSKGYNENTPAFKGIG
HCCCCCCEEEEEEEHHHHHHHHHHHHEEEHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCC
YNEFLLWKSRPCYSLNDIISLINKNSFLYAKRQMTFFAKISNVLWFHPEDDLVSILNLIF
CCEEEEECCCCCCCHHHHHHHHCCCCEEEEHHHHHHHEECCCEEEECCCHHHHHHHHHHH
NVDKEI
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]