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The map label for this gene is amiB [H]

Identifier: 51598921

GI number: 51598921

Start: 705866

End: 706873

Strand: Direct

Name: amiB [H]

Synonym: BG0689

Alternate gene names: 51598921

Gene position: 705866-706873 (Clockwise)

Preceding gene: 51598920

Following gene: 51598922

Centisome position: 78.06

GC content: 25.99

Gene sequence:

>1008_bases
ATGATATTATTGATTAATTTGATTTTGTTTTCATATTTAAGCTTATATTCCAAGACCCTTGATTACTTAAATGTTCTTGA
TTTTTTTGACACCGATGTTTTTAAGTTTGATTTAAACATTGAAAATGATGTTTTTACAATTGAAAATGATAAGGGATATT
TAAATTTTAGGTTAGGTTTTGAATATGCGCTTACATCTTCTGGTTATTATATGTTTGTAGACCCAATTATTGACATTCGT
GGAGAAATTTTAATAAGCCAAAAGGTCTTAAAACAAATTGAAAATTATTTCAATTCTCTTAAAAGTTATAATAAACCCAG
AATTACTTCAATAATCATTGATCCTGGACATGGAGGGCATGATGCTGGTGCGGTTGTGACTTTAAAGATAAATGGTTACG
ATGTTGTGCTTCAAGAAAAAGATTTTGCATTAACTTATTCTATATATTTGTCTAAAATTTTAAGTAATTATTTTATAAAT
AAAAATATTTTGTTAACTCGTGTAAATGACGTTTTTTTAACTTTAAAAGAGCGGTCGGAATTTGCAAATGCAATAAAGCC
AAATTTTCCAAATAATGTCATATTTTTGTCCATACATGCTAATGATGCTCCAAATAATGAAGCTAGAGGAGTTGAGTTTT
GGTATCTTCCTAAGGATTCAAAAAGAGAGGTTATTAAACATTTTAAGGGATATGATATTAAAGGTAATAGATACTTAAGT
GAGCTTAATGATATACTAGATATTAAATATAAATATGAATCTAAAAGATTGGCTGAAATTTTGTATAAAGTATTTAAAAA
TGAGTTAAACGAAACTAATATTAGGCCAATTAGAGAAGAGCAGTGGTTTGTAATAAAAAATAGCAGTATGCCTGCTGTAT
TGATTGAAATGGGTTTTTTGTCCAATATTTTAGATGCTAAATTAATTTTGGATTATAATTACATGAGCAAGTTTAATATC
CTAATACTTAAATCTTTGGTAAAATTTATAAATTTTTATGAAAAATAA

Upstream 100 bases:

>100_bases
TTTCTCTTTCAAAAAGAGTTTAAAGAAATAAAAGGTGAAATTGCTAAAAATAGCTTAAGATTCAAAGGAGACTTAAAGAG
TGCCATCGAGCTTTGGGAAA

Downstream 100 bases:

>100_bases
TATTTCGGATGTATTTTTTAAATATAATACAGTGATTTATAATTTTTTAAATTCAAAAAAAGAGCATACGGTTAGATTGC
TTTTAGTGATTTTGGCAGCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MILLINLILFSYLSLYSKTLDYLNVLDFFDTDVFKFDLNIENDVFTIENDKGYLNFRLGFEYALTSSGYYMFVDPIIDIR
GEILISQKVLKQIENYFNSLKSYNKPRITSIIIDPGHGGHDAGAVVTLKINGYDVVLQEKDFALTYSIYLSKILSNYFIN
KNILLTRVNDVFLTLKERSEFANAIKPNFPNNVIFLSIHANDAPNNEARGVEFWYLPKDSKREVIKHFKGYDIKGNRYLS
ELNDILDIKYKYESKRLAEILYKVFKNELNETNIRPIREEQWFVIKNSSMPAVLIEMGFLSNILDAKLILDYNYMSKFNI
LILKSLVKFINFYEK

Sequences:

>Translated_335_residues
MILLINLILFSYLSLYSKTLDYLNVLDFFDTDVFKFDLNIENDVFTIENDKGYLNFRLGFEYALTSSGYYMFVDPIIDIR
GEILISQKVLKQIENYFNSLKSYNKPRITSIIIDPGHGGHDAGAVVTLKINGYDVVLQEKDFALTYSIYLSKILSNYFIN
KNILLTRVNDVFLTLKERSEFANAIKPNFPNNVIFLSIHANDAPNNEARGVEFWYLPKDSKREVIKHFKGYDIKGNRYLS
ELNDILDIKYKYESKRLAEILYKVFKNELNETNIRPIREEQWFVIKNSSMPAVLIEMGFLSNILDAKLILDYNYMSKFNI
LILKSLVKFINFYEK
>Mature_335_residues
MILLINLILFSYLSLYSKTLDYLNVLDFFDTDVFKFDLNIENDVFTIENDKGYLNFRLGFEYALTSSGYYMFVDPIIDIR
GEILISQKVLKQIENYFNSLKSYNKPRITSIIIDPGHGGHDAGAVVTLKINGYDVVLQEKDFALTYSIYLSKILSNYFIN
KNILLTRVNDVFLTLKERSEFANAIKPNFPNNVIFLSIHANDAPNNEARGVEFWYLPKDSKREVIKHFKGYDIKGNRYLS
ELNDILDIKYKYESKRLAEILYKVFKNELNETNIRPIREEQWFVIKNSSMPAVLIEMGFLSNILDAKLILDYNYMSKFNI
LILKSLVKFINFYEK

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=234, Percent_Identity=26.0683760683761, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788776, Length=278, Percent_Identity=24.4604316546763, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01520 Amidase_3; PF01476 LysM [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 39208; Mature: 39208

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLINLILFSYLSLYSKTLDYLNVLDFFDTDVFKFDLNIENDVFTIENDKGYLNFRLGF
CEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCEEEEEEEE
EYALTSSGYYMFVDPIIDIRGEILISQKVLKQIENYFNSLKSYNKPRITSIIIDPGHGGH
EEEEECCCEEEEECCEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
DAGAVVTLKINGYDVVLQEKDFALTYSIYLSKILSNYFINKNILLTRVNDVFLTLKERSE
CCCEEEEEEECCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCEEEEEECEEEEEEHHHHH
FANAIKPNFPNNVIFLSIHANDAPNNEARGVEFWYLPKDSKREVIKHFKGYDIKGNRYLS
HHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHH
ELNDILDIKYKYESKRLAEILYKVFKNELNETNIRPIREEQWFVIKNSSMPAVLIEMGFL
HHHHHHEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEHHHHH
SNILDAKLILDYNYMSKFNILILKSLVKFINFYEK
HHHHCEEEEEEECHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILLINLILFSYLSLYSKTLDYLNVLDFFDTDVFKFDLNIENDVFTIENDKGYLNFRLGF
CEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEECCCCEEEEEEEE
EYALTSSGYYMFVDPIIDIRGEILISQKVLKQIENYFNSLKSYNKPRITSIIIDPGHGGH
EEEEECCCEEEEECCEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
DAGAVVTLKINGYDVVLQEKDFALTYSIYLSKILSNYFINKNILLTRVNDVFLTLKERSE
CCCEEEEEEECCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCEEEEEECEEEEEEHHHHH
FANAIKPNFPNNVIFLSIHANDAPNNEARGVEFWYLPKDSKREVIKHFKGYDIKGNRYLS
HHHHCCCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHH
ELNDILDIKYKYESKRLAEILYKVFKNELNETNIRPIREEQWFVIKNSSMPAVLIEMGFL
HHHHHHEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEHHHHH
SNILDAKLILDYNYMSKFNILILKSLVKFINFYEK
HHHHCEEEEEEECHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]