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The map label for this gene is mltD [C]

Identifier: 51598878

GI number: 51598878

Start: 656749

End: 658836

Strand: Direct

Name: mltD [C]

Synonym: BG0644

Alternate gene names: 51598878

Gene position: 656749-658836 (Clockwise)

Preceding gene: 51598877

Following gene: 51598882

Centisome position: 72.63

GC content: 28.83

Gene sequence:

>2088_bases
TTGTTCAAAATACTGGTATTAAGGAAAAGTAAAACTCTTGGTGTTTTACAAATTATAGGACTTATTATTTTATTTTATTT
TTCAATAATAAGTCCTACTAATCTTAATGCTGATTTTGAGTATAAGGTTGTCAAAGGAGATACTCTTTTTTCAATTGCAA
TTAAATATAAAGCTAAATTAAATGATCTTAAAAGGATTAATAAACTTAATGTTGACAATATTAGGGTGGGTCAAATATTA
ATTATTCCAAGTGATTCTAATTTAGATCAGAACATTACTCATAAAGTCAATAATTCTTTTAATTCGTTAGAATCTGTTAA
GAAAGGGGTAATTCTTTATACCACAAAAGAGAATGACACTGTTGAGAGCGTTTCAAAGCTTGTTGGAATAAGCCAAGAAG
AGATAATAGCTTGGAATGATTTACGATCTAAAGACCTTAAAGTTGGAATGAAGCTTGTATTAACTGAGCCTGATTTTTTA
AAGCCTTATGTAGTTAAAAAAGGCGATTCACTTTCAAAACTTGCTCAGGATTTTGATATTAGTTCTAAGGATATTTTAAA
ATTTAATTTTCTTAATGATGATAAATTAAAGATTGGCCAACGTCTTTTTTTAAAGAAAGCGACCGAGAGTGTTAATTTTC
ATTATGTTAAAAAAGGAGAAACTCTTGGTAGAATAGCTTATATCTATAGCCTTACTGCTAGGGATCTTGTAGCTCTTAAT
GGCAATCGAGCTGTTAATCTTAAAGCAGGCTCATTGCTAAATGTTTTAAAGATTGTAAATAATGATTTAGAAAAGCCTTT
TGAGAAAGATTTAAAAATTGAGGAAAAAACAGGTAATCAGTTTATCTATCATTCAGTTGCTGTGGGGGAAACTTTATATA
GCATTGCTAGGCACTATGGTGTTTTAATTGAGGATCTTAAAAATTGGAATAATTTAAGTAGTAACAATATTATGCACGAT
CAAAAATTGAAAATTTTTGATAAAAAACTTTTACTTGATTCTTCTATAATTAAATCTAAAAACGATTCAGATATTAAAAA
CAAAGTTGATTCTAGCGCTAATTCTTCTAATAAAGTTCAAACAATAGTTAATTTTCCCTCAAGCAAAAACAAAAAGCTAA
ATTTAAATACTTTTGGAACTACTGTCAATCAAGATCTTTTTGATATTAGTTCTTTGGTTATTTTAGACTCCAAAATACCA
ATATTTGAAGTTGTTGGTGATTTCTATTATTGGTATAGACCTAAAAAGATAAGTCAGCCAAGTGAGTTTTATTCAGAAGA
TTGGCATTCTCCTTTAAATTCTTATAAAAAGGCGACTCAACTTTTCAAAAGTTTTGAAAAGTTGGTAAATTCTAGGTTTA
ATAAAGGATCAAGACTTAAAGATAAGTTAATAATTCTTGATCCCGGACACGGGGGCCTTGATCCTGGAGCTATTGTTAAA
TCTAGAGATGGTCTTGGAAACGAAGTTTTTGTTGTTGAAGATGAATATGTTTATGATATTGCTTTAAGGCTTTATGTATA
CCTTAAACAAGAAGGGGCCAATGTTGAGCTTACTATTTTAGCCCCCGATCATTTAATTAGGAATAGTGTTTTTGCTAACA
ATACTTTTGTTAATGTTAAAAATGAGATTTACAATGATTATGACCTAAACAAAAATGATACTGTTGATTCCTGGATAAGC
GGTACTCCATCGGGCCTTAAAAAAAGATTGGTCGTTGTTAAAAACATTGTTAGTAAATATAAAAATATTAAAGATAAGGA
TATAGCCTTTTTTAGTTTACATGCAGATAATAGTGTTGGTGCGCCTAAGAGTATGGGGTTTTATTATCAAAAAGATGACG
AAAAAAAATATGATATTCATTCAAAATCTGTAGCAGAAAAAATTACAGAAGGAATGAAAAGAAGTTTTTATATTAAGGGT
CAAAATCTCCATGTTTTAAGAAATAATATAGTAATGACGAAGCTTTTAATAGAAGTAAGAAATTTAGCATTTCCAGAGGA
AGCTTGGTCTATTAGGTCTTCTAAGCTTAGAGATCAAGATTCCAAAATTCTTGCTAATGGGATTTTAAAAATATTAGAAA
ATAATTGA

Upstream 100 bases:

>100_bases
ATTGCTGATTTATATTTTGACGATGGGCATTTTTATATTTCCAAAAGATATACTTTTTTCTTCAAAAAATATGATTATTA
TTGGATAATTTATGACTACA

Downstream 100 bases:

>100_bases
CAAAAATTTTTAAATTTAAGATAATATTCCTAGTGTTCAATTTCCCCTATAGCTCAGTGGTAGAGCGGGTGGCTGTTAAC
CACTAGGTCGGAGGTTCAAG

Product: N-acetylmuramoyl-L-alanine amidase, putative

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Beta-glycosidase; Peptidoglycan hydrolase [H]

Number of amino acids: Translated: 695; Mature: 695

Protein sequence:

>695_residues
MFKILVLRKSKTLGVLQIIGLIILFYFSIISPTNLNADFEYKVVKGDTLFSIAIKYKAKLNDLKRINKLNVDNIRVGQIL
IIPSDSNLDQNITHKVNNSFNSLESVKKGVILYTTKENDTVESVSKLVGISQEEIIAWNDLRSKDLKVGMKLVLTEPDFL
KPYVVKKGDSLSKLAQDFDISSKDILKFNFLNDDKLKIGQRLFLKKATESVNFHYVKKGETLGRIAYIYSLTARDLVALN
GNRAVNLKAGSLLNVLKIVNNDLEKPFEKDLKIEEKTGNQFIYHSVAVGETLYSIARHYGVLIEDLKNWNNLSSNNIMHD
QKLKIFDKKLLLDSSIIKSKNDSDIKNKVDSSANSSNKVQTIVNFPSSKNKKLNLNTFGTTVNQDLFDISSLVILDSKIP
IFEVVGDFYYWYRPKKISQPSEFYSEDWHSPLNSYKKATQLFKSFEKLVNSRFNKGSRLKDKLIILDPGHGGLDPGAIVK
SRDGLGNEVFVVEDEYVYDIALRLYVYLKQEGANVELTILAPDHLIRNSVFANNTFVNVKNEIYNDYDLNKNDTVDSWIS
GTPSGLKKRLVVVKNIVSKYKNIKDKDIAFFSLHADNSVGAPKSMGFYYQKDDEKKYDIHSKSVAEKITEGMKRSFYIKG
QNLHVLRNNIVMTKLLIEVRNLAFPEEAWSIRSSKLRDQDSKILANGILKILENN

Sequences:

>Translated_695_residues
MFKILVLRKSKTLGVLQIIGLIILFYFSIISPTNLNADFEYKVVKGDTLFSIAIKYKAKLNDLKRINKLNVDNIRVGQIL
IIPSDSNLDQNITHKVNNSFNSLESVKKGVILYTTKENDTVESVSKLVGISQEEIIAWNDLRSKDLKVGMKLVLTEPDFL
KPYVVKKGDSLSKLAQDFDISSKDILKFNFLNDDKLKIGQRLFLKKATESVNFHYVKKGETLGRIAYIYSLTARDLVALN
GNRAVNLKAGSLLNVLKIVNNDLEKPFEKDLKIEEKTGNQFIYHSVAVGETLYSIARHYGVLIEDLKNWNNLSSNNIMHD
QKLKIFDKKLLLDSSIIKSKNDSDIKNKVDSSANSSNKVQTIVNFPSSKNKKLNLNTFGTTVNQDLFDISSLVILDSKIP
IFEVVGDFYYWYRPKKISQPSEFYSEDWHSPLNSYKKATQLFKSFEKLVNSRFNKGSRLKDKLIILDPGHGGLDPGAIVK
SRDGLGNEVFVVEDEYVYDIALRLYVYLKQEGANVELTILAPDHLIRNSVFANNTFVNVKNEIYNDYDLNKNDTVDSWIS
GTPSGLKKRLVVVKNIVSKYKNIKDKDIAFFSLHADNSVGAPKSMGFYYQKDDEKKYDIHSKSVAEKITEGMKRSFYIKG
QNLHVLRNNIVMTKLLIEVRNLAFPEEAWSIRSSKLRDQDSKILANGILKILENN
>Mature_695_residues
MFKILVLRKSKTLGVLQIIGLIILFYFSIISPTNLNADFEYKVVKGDTLFSIAIKYKAKLNDLKRINKLNVDNIRVGQIL
IIPSDSNLDQNITHKVNNSFNSLESVKKGVILYTTKENDTVESVSKLVGISQEEIIAWNDLRSKDLKVGMKLVLTEPDFL
KPYVVKKGDSLSKLAQDFDISSKDILKFNFLNDDKLKIGQRLFLKKATESVNFHYVKKGETLGRIAYIYSLTARDLVALN
GNRAVNLKAGSLLNVLKIVNNDLEKPFEKDLKIEEKTGNQFIYHSVAVGETLYSIARHYGVLIEDLKNWNNLSSNNIMHD
QKLKIFDKKLLLDSSIIKSKNDSDIKNKVDSSANSSNKVQTIVNFPSSKNKKLNLNTFGTTVNQDLFDISSLVILDSKIP
IFEVVGDFYYWYRPKKISQPSEFYSEDWHSPLNSYKKATQLFKSFEKLVNSRFNKGSRLKDKLIILDPGHGGLDPGAIVK
SRDGLGNEVFVVEDEYVYDIALRLYVYLKQEGANVELTILAPDHLIRNSVFANNTFVNVKNEIYNDYDLNKNDTVDSWIS
GTPSGLKKRLVVVKNIVSKYKNIKDKDIAFFSLHADNSVGAPKSMGFYYQKDDEKKYDIHSKSVAEKITEGMKRSFYIKG
QNLHVLRNNIVMTKLLIEVRNLAFPEEAWSIRSSKLRDQDSKILANGILKILENN

Specific function: Hydrolyzes the cell wall of E.faecalis and M.lysodeikticus. May play an important role in cell wall growth and cell separation [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 LysM repeats [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013338
- InterPro:   IPR002901
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF01476 LysM [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 79084; Mature: 79084

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKILVLRKSKTLGVLQIIGLIILFYFSIISPTNLNADFEYKVVKGDTLFSIAIKYKAKL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEEEEEHHHH
NDLKRINKLNVDNIRVGQILIIPSDSNLDQNITHKVNNSFNSLESVKKGVILYTTKENDT
HHHHHHHCCCCCCEEEEEEEEEECCCCCCCHHHHHHCCCHHHHHHHHCCEEEEEECCCCH
VESVSKLVGISQEEIIAWNDLRSKDLKVGMKLVLTEPDFLKPYVVKKGDSLSKLAQDFDI
HHHHHHHHCCCHHHEEEECCCCCCHHHCCEEEEEECCCCCCCEEEECCCHHHHHHHHCCC
SSKDILKFNFLNDDKLKIGQRLFLKKATESVNFHYVKKGETLGRIAYIYSLTARDLVALN
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHEEEEEEEEHHHHEEEEC
GNRAVNLKAGSLLNVLKIVNNDLEKPFEKDLKIEEKTGNQFIYHSVAVGETLYSIARHYG
CCEEEEEECHHHHHHHHHHHHHHCCHHHHCCEEEECCCCEEEEEEHHHHHHHHHHHHHHH
VLIEDLKNWNNLSSNNIMHDQKLKIFDKKLLLDSSIIKSKNDSDIKNKVDSSANSSNKVQ
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEE
TIVNFPSSKNKKLNLNTFGTTVNQDLFDISSLVILDSKIPIFEVVGDFYYWYRPKKISQP
EEEECCCCCCCEEEEEECCCCCCCHHHHHHEEEEEECCCCHHHHHHHHHHEECCCCCCCC
SEFYSEDWHSPLNSYKKATQLFKSFEKLVNSRFNKGSRLKDKLIILDPGHGGLDPGAIVK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEE
SRDGLGNEVFVVEDEYVYDIALRLYVYLKQEGANVELTILAPDHLIRNSVFANNTFVNVK
CCCCCCCEEEEEECCHHHHHHHHHEEEEEECCCCEEEEEECCHHHHHCCEECCCCEEEEH
NEIYNDYDLNKNDTVDSWISGTPSGLKKRLVVVKNIVSKYKNIKDKDIAFFSLHADNSVG
HHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
APKSMGFYYQKDDEKKYDIHSKSVAEKITEGMKRSFYIKGQNLHVLRNNIVMTKLLIEVR
CCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCEEEEHHHHHHHHHHHHHH
NLAFPEEAWSIRSSKLRDQDSKILANGILKILENN
HCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKILVLRKSKTLGVLQIIGLIILFYFSIISPTNLNADFEYKVVKGDTLFSIAIKYKAKL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEEEEEHHHH
NDLKRINKLNVDNIRVGQILIIPSDSNLDQNITHKVNNSFNSLESVKKGVILYTTKENDT
HHHHHHHCCCCCCEEEEEEEEEECCCCCCCHHHHHHCCCHHHHHHHHCCEEEEEECCCCH
VESVSKLVGISQEEIIAWNDLRSKDLKVGMKLVLTEPDFLKPYVVKKGDSLSKLAQDFDI
HHHHHHHHCCCHHHEEEECCCCCCHHHCCEEEEEECCCCCCCEEEECCCHHHHHHHHCCC
SSKDILKFNFLNDDKLKIGQRLFLKKATESVNFHYVKKGETLGRIAYIYSLTARDLVALN
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHEEEEEEEEHHHHEEEEC
GNRAVNLKAGSLLNVLKIVNNDLEKPFEKDLKIEEKTGNQFIYHSVAVGETLYSIARHYG
CCEEEEEECHHHHHHHHHHHHHHCCHHHHCCEEEECCCCEEEEEEHHHHHHHHHHHHHHH
VLIEDLKNWNNLSSNNIMHDQKLKIFDKKLLLDSSIIKSKNDSDIKNKVDSSANSSNKVQ
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEE
TIVNFPSSKNKKLNLNTFGTTVNQDLFDISSLVILDSKIPIFEVVGDFYYWYRPKKISQP
EEEECCCCCCCEEEEEECCCCCCCHHHHHHEEEEEECCCCHHHHHHHHHHEECCCCCCCC
SEFYSEDWHSPLNSYKKATQLFKSFEKLVNSRFNKGSRLKDKLIILDPGHGGLDPGAIVK
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEE
SRDGLGNEVFVVEDEYVYDIALRLYVYLKQEGANVELTILAPDHLIRNSVFANNTFVNVK
CCCCCCCEEEEEECCHHHHHHHHHEEEEEECCCCEEEEEECCHHHHHCCEECCCCEEEEH
NEIYNDYDLNKNDTVDSWISGTPSGLKKRLVVVKNIVSKYKNIKDKDIAFFSLHADNSVG
HHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCC
APKSMGFYYQKDDEKKYDIHSKSVAEKITEGMKRSFYIKGQNLHVLRNNIVMTKLLIEVR
CCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCEEEEHHHHHHHHHHHHHH
NLAFPEEAWSIRSSKLRDQDSKILANGILKILENN
HCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1679432; 12663927 [H]