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The map label for this gene is rep [H]

Identifier: 51598860

GI number: 51598860

Start: 634435

End: 636411

Strand: Direct

Name: rep [H]

Synonym: BG0620

Alternate gene names: 51598860

Gene position: 634435-636411 (Clockwise)

Preceding gene: 51598859

Following gene: 51598861

Centisome position: 70.16

GC content: 27.42

Gene sequence:

>1977_bases
TTGCCTGATGATTTTTTTAAAAATTCTCTAAATCAATTTCAATATGAAGCGGTTACCACTATTGAGGGTGCTCTTTTAAT
TATTGCTGGTGCTGGCAGTGGAAAAACAAGGGTTGTTACTCATAGAATAGCTTATTTGCTTCTAAAAGGCATTGCTCAGA
AGGAAATTTTAGCCTTAACTTTTACTAATAAGGCGGCTAATGAAATGAAAGATAGAATTAAAAAAATTTTGAAAAATCCT
CTTAGCAATCTTATGATCTCAACTTTTCATGCTTTTGGACTTTTCTTTTTGAAAGAAAATTATAAGTTGTTAGGATATAA
AAAAAACTTTAGCATTTATGATGATAATGATAGAATTTCTCTTCTTAAAGAGATTTTACTTGATGAAGGTCTTTTTAATA
AAAAAGTTTCTTTAAGTTCGCTTAGCAATGCTATTTCACTTTTAAAAAATGGTATTCTCACTTTTGATGATTTAAAGGAA
GAAGATATACATATTTTTAGACTTTATGAAGATCGATTAAGGCTTTATAATTCTTTTGATTTTGACGATTTAATTTTAAA
GCCAAAAGAATTGTTAAGTAATAATTCTGACATTAGAAGTAAATATTCTAAAAGATATAAGTATGTTTTGATTGACGAGT
TTCAAGATACTTCTTTGCTTCAATATAATTTTATTCGCCTTTTAATAAATCATAGTAATTTATGTTGCGTTGGGGATGAT
GATCAATCGATATATTCTTGGCGTGGGGCTAGCTATAACAATATTTTGCAATTTGAAAAAGATTACAGTGCTAAAGAAAT
AAAGCTTGAACAAAATTATCGTTCTGCAAAAAATATTTTAGATGTTGCCAATTCTGTGATTTTAAATAATAAAAATCGAA
AGGAAAAGACCTTATGGTCTTCAAAGATGTGTAGCAAAGTTATAGATGTTTTTATGTTTGATGATGAGATTCAAGAATCT
GAGTTTGTTGCAAGTCAAATAATAGAGCTTTCAAGGATAGAAGGTTTTAAATCTAAAAAAATAGGAGTTATTATGAGAAC
TAATGCTCTTTTTAGAAACGTTGAGATGATTTTTAAAAGACAGGGCATAAAATATAAGGTTTCAGGGGGAACATCTTTTT
TCCAGAGAAAAGAAATAAAAGATATTATTTCTTATTTAAATGTAATAATAAATCCTAAAAGTGATTATGATTTGCTTAGA
ATTATAAATGTTCCAAGAAGGGGAATTGGCAAGGAATATTTGAAAAAAATTAGAGATATAGCAGATAAGAAAGGTTGCTG
TATTTATGATGCTCTTTGTGATATTGTTTTTTCTTTTACAAGCACTTCTAGTTACGACAAAGCTTTAAATAAGCAGGTCA
TTGAAAGTATAGAAGATTTTGTGTCTTTTATTGAAGAGTATCAATATAAATTTAGTATAACAAAAAACACTTATTCTAAT
ATAATCAAAGAAATGATAGAAAGTATTGAATATTGGGGATTTCTAGTTAATGAAAATCCCAATTCAATTAAAGTGGCTGA
GTATAAATATCAAAATATAGAAGGGTTTTTAAGTATAATTAAAAATTGGGAATCCAAGCAATTTGGCGAATTGAGAGATT
TAAGCAGTTTTTTAAACTATATACTTCTTCAATCTAATGAAGTTAGTGAGGAATCTGAAAATATTAATATTAATTTAATG
ACAGTGCATTCTGCTAAAGGTTTAGAATTTGATTATGTATTTTTTATTGCTGTTGAAGACAATATTATTCCGCATCATAG
AATAATTGAAGAGAGCGAGATTGGTTTAGAGGAGGAAAGGCGAGTTTTTTATGTTGCATTAACTCGTGCAAAAGATTCTC
TTATTATTACTATGGCCAATAAGCGAAAAAAAGATAGGCAAATTTTTGATCAGCTGCCTTCAAGATTTATTTCAGAAATT
CCTAAAGAATTTTTAAATTTTAACTATTATGCAGATTTTTCTAAAAATAAAGCTTGA

Upstream 100 bases:

>100_bases
CTAAAGATTTTGATCAATCTAAGTCACGAAAGATTTTTGCTTTTCCTGAAAACTTTAAAGTTATTGTGGAATATCCAGAT
GGAACAAAGGTTTTTTAATT

Downstream 100 bases:

>100_bases
TTAGTATTATTGAATAAATTTTAATATTGATTTAGTATATTATATTATTAATTATAATTAAGTCTAAGTATTTTTATGGA
TTTAATATAATCACATAATT

Product: rep helicase, single-stranded DNA-dependent ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MPDDFFKNSLNQFQYEAVTTIEGALLIIAGAGSGKTRVVTHRIAYLLLKGIAQKEILALTFTNKAANEMKDRIKKILKNP
LSNLMISTFHAFGLFFLKENYKLLGYKKNFSIYDDNDRISLLKEILLDEGLFNKKVSLSSLSNAISLLKNGILTFDDLKE
EDIHIFRLYEDRLRLYNSFDFDDLILKPKELLSNNSDIRSKYSKRYKYVLIDEFQDTSLLQYNFIRLLINHSNLCCVGDD
DQSIYSWRGASYNNILQFEKDYSAKEIKLEQNYRSAKNILDVANSVILNNKNRKEKTLWSSKMCSKVIDVFMFDDEIQES
EFVASQIIELSRIEGFKSKKIGVIMRTNALFRNVEMIFKRQGIKYKVSGGTSFFQRKEIKDIISYLNVIINPKSDYDLLR
IINVPRRGIGKEYLKKIRDIADKKGCCIYDALCDIVFSFTSTSSYDKALNKQVIESIEDFVSFIEEYQYKFSITKNTYSN
IIKEMIESIEYWGFLVNENPNSIKVAEYKYQNIEGFLSIIKNWESKQFGELRDLSSFLNYILLQSNEVSEESENININLM
TVHSAKGLEFDYVFFIAVEDNIIPHHRIIEESEIGLEEERRVFYVALTRAKDSLIITMANKRKKDRQIFDQLPSRFISEI
PKEFLNFNYYADFSKNKA

Sequences:

>Translated_658_residues
MPDDFFKNSLNQFQYEAVTTIEGALLIIAGAGSGKTRVVTHRIAYLLLKGIAQKEILALTFTNKAANEMKDRIKKILKNP
LSNLMISTFHAFGLFFLKENYKLLGYKKNFSIYDDNDRISLLKEILLDEGLFNKKVSLSSLSNAISLLKNGILTFDDLKE
EDIHIFRLYEDRLRLYNSFDFDDLILKPKELLSNNSDIRSKYSKRYKYVLIDEFQDTSLLQYNFIRLLINHSNLCCVGDD
DQSIYSWRGASYNNILQFEKDYSAKEIKLEQNYRSAKNILDVANSVILNNKNRKEKTLWSSKMCSKVIDVFMFDDEIQES
EFVASQIIELSRIEGFKSKKIGVIMRTNALFRNVEMIFKRQGIKYKVSGGTSFFQRKEIKDIISYLNVIINPKSDYDLLR
IINVPRRGIGKEYLKKIRDIADKKGCCIYDALCDIVFSFTSTSSYDKALNKQVIESIEDFVSFIEEYQYKFSITKNTYSN
IIKEMIESIEYWGFLVNENPNSIKVAEYKYQNIEGFLSIIKNWESKQFGELRDLSSFLNYILLQSNEVSEESENININLM
TVHSAKGLEFDYVFFIAVEDNIIPHHRIIEESEIGLEEERRVFYVALTRAKDSLIITMANKRKKDRQIFDQLPSRFISEI
PKEFLNFNYYADFSKNKA
>Mature_657_residues
PDDFFKNSLNQFQYEAVTTIEGALLIIAGAGSGKTRVVTHRIAYLLLKGIAQKEILALTFTNKAANEMKDRIKKILKNPL
SNLMISTFHAFGLFFLKENYKLLGYKKNFSIYDDNDRISLLKEILLDEGLFNKKVSLSSLSNAISLLKNGILTFDDLKEE
DIHIFRLYEDRLRLYNSFDFDDLILKPKELLSNNSDIRSKYSKRYKYVLIDEFQDTSLLQYNFIRLLINHSNLCCVGDDD
QSIYSWRGASYNNILQFEKDYSAKEIKLEQNYRSAKNILDVANSVILNNKNRKEKTLWSSKMCSKVIDVFMFDDEIQESE
FVASQIIELSRIEGFKSKKIGVIMRTNALFRNVEMIFKRQGIKYKVSGGTSFFQRKEIKDIISYLNVIINPKSDYDLLRI
INVPRRGIGKEYLKKIRDIADKKGCCIYDALCDIVFSFTSTSSYDKALNKQVIESIEDFVSFIEEYQYKFSITKNTYSNI
IKEMIESIEYWGFLVNENPNSIKVAEYKYQNIEGFLSIIKNWESKQFGELRDLSSFLNYILLQSNEVSEESENININLMT
VHSAKGLEFDYVFFIAVEDNIIPHHRIIEESEIGLEEERRVFYVALTRAKDSLIITMANKRKKDRQIFDQLPSRFISEIP
KEFLNFNYYADFSKNKA

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=652, Percent_Identity=37.7300613496933, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI2367296, Length=651, Percent_Identity=32.258064516129, Blast_Score=344, Evalue=1e-95,
Organism=Escherichia coli, GI1787196, Length=348, Percent_Identity=28.735632183908, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=715, Percent_Identity=27.1328671328671, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6324477, Length=678, Percent_Identity=23.7463126843658, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76776; Mature: 76645

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDDFFKNSLNQFQYEAVTTIEGALLIIAGAGSGKTRVVTHRIAYLLLKGIAQKEILALT
CCCHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
FTNKAANEMKDRIKKILKNPLSNLMISTFHAFGLFFLKENYKLLGYKKNFSIYDDNDRIS
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCEECCCCHHH
LLKEILLDEGLFNKKVSLSSLSNAISLLKNGILTFDDLKEEDIHIFRLYEDRLRLYNSFD
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCC
FDDLILKPKELLSNNSDIRSKYSKRYKYVLIDEFQDTSLLQYNFIRLLINHSNLCCVGDD
CHHHHCCCHHHHCCCCHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHEECCCCEEEECCC
DQSIYSWRGASYNNILQFEKDYSAKEIKLEQNYRSAKNILDVANSVILNNKNRKEKTLWS
CCCHHEECCCCCCCCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SKMCSKVIDVFMFDDEIQESEFVASQIIELSRIEGFKSKKIGVIMRTNALFRNVEMIFKR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHH
QGIKYKVSGGTSFFQRKEIKDIISYLNVIINPKSDYDLLRIINVPRRGIGKEYLKKIRDI
CCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCHHHEECCCCCCCCHHHHHHHHHH
ADKKGCCIYDALCDIVFSFTSTSSYDKALNKQVIESIEDFVSFIEEYQYKFSITKNTYSN
HCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHH
IIKEMIESIEYWGFLVNENPNSIKVAEYKYQNIEGFLSIIKNWESKQFGELRDLSSFLNY
HHHHHHHHHHHCEEEEECCCCEEEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ILLQSNEVSEESENININLMTVHSAKGLEFDYVFFIAVEDNIIPHHRIIEESEIGLEEER
HHCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEEEEEEECCCCCCHHHCCHHHCCCCCCC
RVFYVALTRAKDSLIITMANKRKKDRQIFDQLPSRFISEIPKEFLNFNYYADFSKNKA
EEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC
>Mature Secondary Structure 
PDDFFKNSLNQFQYEAVTTIEGALLIIAGAGSGKTRVVTHRIAYLLLKGIAQKEILALT
CCHHHHHHHHHHHHHHHHEECCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
FTNKAANEMKDRIKKILKNPLSNLMISTFHAFGLFFLKENYKLLGYKKNFSIYDDNDRIS
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCEECCCCHHH
LLKEILLDEGLFNKKVSLSSLSNAISLLKNGILTFDDLKEEDIHIFRLYEDRLRLYNSFD
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEHHHHHHHCCCC
FDDLILKPKELLSNNSDIRSKYSKRYKYVLIDEFQDTSLLQYNFIRLLINHSNLCCVGDD
CHHHHCCCHHHHCCCCHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHEECCCCEEEECCC
DQSIYSWRGASYNNILQFEKDYSAKEIKLEQNYRSAKNILDVANSVILNNKNRKEKTLWS
CCCHHEECCCCCCCCEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SKMCSKVIDVFMFDDEIQESEFVASQIIELSRIEGFKSKKIGVIMRTNALFRNVEMIFKR
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHH
QGIKYKVSGGTSFFQRKEIKDIISYLNVIINPKSDYDLLRIINVPRRGIGKEYLKKIRDI
CCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCHHHEECCCCCCCCHHHHHHHHHH
ADKKGCCIYDALCDIVFSFTSTSSYDKALNKQVIESIEDFVSFIEEYQYKFSITKNTYSN
HCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHH
IIKEMIESIEYWGFLVNENPNSIKVAEYKYQNIEGFLSIIKNWESKQFGELRDLSSFLNY
HHHHHHHHHHHCEEEEECCCCEEEEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
ILLQSNEVSEESENININLMTVHSAKGLEFDYVFFIAVEDNIIPHHRIIEESEIGLEEER
HHCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEEEEEEECCCCCCHHHCCHHHCCCCCCC
RVFYVALTRAKDSLIITMANKRKKDRQIFDQLPSRFISEIPKEFLNFNYYADFSKNKA
EEEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]