Click here to switch to the map view.

The map label for this gene is dacA [H]

Identifier: 51598858

GI number: 51598858

Start: 632719

End: 633924

Strand: Direct

Name: dacA [H]

Synonym: BG0618

Alternate gene names: 51598858

Gene position: 632719-633924 (Clockwise)

Preceding gene: 51598855

Following gene: 51598859

Centisome position: 69.97

GC content: 28.36

Gene sequence:

>1206_bases
ATGAATAGTATATGTGTTATTAAAAAATTGCTATTAACTTTATTTTTAATTTTTTTCCTGCTTTTTGATGATCTTTTTGC
AGCTAATCTAGCTGAGATTAATGAATTATCAAAGCATGCAAAATCAATAGTTTTGATGGATTTTGACACTAAGCGGATAC
TTTATTCTAAGAATCCCAATTTGGTTTTTCCTCCTGCATCGCTTACAAAGATTGTTACAATTTATACGGCTTTAATTGAA
GCTGAAAAGCGAAATATAAAGTTAAAAAGCATAGTCCCTATTAGTGATTCTGCTTCATATTATAATGCGCCTTTAAATTC
TTCTTTAATGTTTTTAGAAAAAGGTCAAATTGTTAATTTTGAAGAGATTTTAAAGGGGCTTTCAGTTTCGTCAGGCAATG
ATGCTTCTATTGCAATTGCTGAGTTTGTAGTAGGCGATTTAAATAGTTTTGTTAATTTGATGAATATTAATGTTTTAAAT
TTAGGACTTTTTAATATGCATTTTGTTGAACCTTCTGGATATAGTAATGAGAATAAAATTACAGCACTAGATATGGCTTT
TTTTGTAAAATCTTATATTGAAAAGTTTAAATTTATGCTTAATATCCATTCTTTAAAGTATTTTGTTTACCCAAAGAGTA
AAAATTTAGGAACTACTTTATCATCAAAGTTTTTAAACTTAAAACAAAAAAATGCTAATTTATTAATATCTGATTATCCT
TATTCAGATGGCCTTAAAACGGGATATATTAAGGAATCAGGCTTAAATCTTGTTGCTACTGCCCAAAAGGATGGAAGAAG
ATTAATAGCAGTTGTATTGGGGGTTAAGAAAGGAATTAATGGGCTTGGAGAGAAGATGAGAGCTTTGATTGCAAAAGATT
TATTTGAGTACGGATTTAATGAATACTCCAAATTCCCTTTAATAGTAAAATTAAAAGAAAAAGTCTATAATGGTACAGTA
GATACAGTTGCTCTTTTTTCTAAAGAGCCTTTTTATTATGTTTTAACAAAAGATGAATTTGATAAACTTAAGATAAGTTA
TACTGTTGATAAGTTGGTTGCTCCCCTTAGTGGGGATATGCCTGTTGGGAGGGCTATGATTTTTTTAGAAAATGAAAAAG
TAGGGGATGTTGCTTTGTTTAGTAGTAAAGTAAATAAATTAGGGTTTTGGCAAGGTCTTTATAAAAGTTTTATAAATTTT
TTTTAA

Upstream 100 bases:

>100_bases
AAAGCTATTATAAAATCATAAGAATTCATAAAAAATGACTCCTTGTTAATTATATTATGATTAACAATCAATAAATAATA
TATAATTTTAATATTACAAC

Downstream 100 bases:

>100_bases
GAGAGTATTAAGTTTTATGTTAAATCATTTTAATTTCAAATTGAAACGAGATGTTACAATAATAGTTCCTGGTGAAGCTT
TTGTTTCAAATAAAAGAGTT

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6b; PBP-6b [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNSICVIKKLLLTLFLIFFLLFDDLFAANLAEINELSKHAKSIVLMDFDTKRILYSKNPNLVFPPASLTKIVTIYTALIE
AEKRNIKLKSIVPISDSASYYNAPLNSSLMFLEKGQIVNFEEILKGLSVSSGNDASIAIAEFVVGDLNSFVNLMNINVLN
LGLFNMHFVEPSGYSNENKITALDMAFFVKSYIEKFKFMLNIHSLKYFVYPKSKNLGTTLSSKFLNLKQKNANLLISDYP
YSDGLKTGYIKESGLNLVATAQKDGRRLIAVVLGVKKGINGLGEKMRALIAKDLFEYGFNEYSKFPLIVKLKEKVYNGTV
DTVALFSKEPFYYVLTKDEFDKLKISYTVDKLVAPLSGDMPVGRAMIFLENEKVGDVALFSSKVNKLGFWQGLYKSFINF
F

Sequences:

>Translated_401_residues
MNSICVIKKLLLTLFLIFFLLFDDLFAANLAEINELSKHAKSIVLMDFDTKRILYSKNPNLVFPPASLTKIVTIYTALIE
AEKRNIKLKSIVPISDSASYYNAPLNSSLMFLEKGQIVNFEEILKGLSVSSGNDASIAIAEFVVGDLNSFVNLMNINVLN
LGLFNMHFVEPSGYSNENKITALDMAFFVKSYIEKFKFMLNIHSLKYFVYPKSKNLGTTLSSKFLNLKQKNANLLISDYP
YSDGLKTGYIKESGLNLVATAQKDGRRLIAVVLGVKKGINGLGEKMRALIAKDLFEYGFNEYSKFPLIVKLKEKVYNGTV
DTVALFSKEPFYYVLTKDEFDKLKISYTVDKLVAPLSGDMPVGRAMIFLENEKVGDVALFSSKVNKLGFWQGLYKSFINF
F
>Mature_401_residues
MNSICVIKKLLLTLFLIFFLLFDDLFAANLAEINELSKHAKSIVLMDFDTKRILYSKNPNLVFPPASLTKIVTIYTALIE
AEKRNIKLKSIVPISDSASYYNAPLNSSLMFLEKGQIVNFEEILKGLSVSSGNDASIAIAEFVVGDLNSFVNLMNINVLN
LGLFNMHFVEPSGYSNENKITALDMAFFVKSYIEKFKFMLNIHSLKYFVYPKSKNLGTTLSSKFLNLKQKNANLLISDYP
YSDGLKTGYIKESGLNLVATAQKDGRRLIAVVLGVKKGINGLGEKMRALIAKDLFEYGFNEYSKFPLIVKLKEKVYNGTV
DTVALFSKEPFYYVLTKDEFDKLKISYTVDKLVAPLSGDMPVGRAMIFLENEKVGDVALFSSKVNKLGFWQGLYKSFINF
F

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=385, Percent_Identity=29.6103896103896, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1787062, Length=381, Percent_Identity=31.496062992126, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786851, Length=334, Percent_Identity=31.7365269461078, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI87082054, Length=148, Percent_Identity=33.1081081081081, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 45123; Mature: 45123

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSICVIKKLLLTLFLIFFLLFDDLFAANLAEINELSKHAKSIVLMDFDTKRILYSKNPN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEECCCC
LVFPPASLTKIVTIYTALIEAEKRNIKLKSIVPISDSASYYNAPLNSSLMFLEKGQIVNF
EEECCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCEECH
EEILKGLSVSSGNDASIAIAEFVVGDLNSFVNLMNINVLNLGLFNMHFVEPSGYSNENKI
HHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCCCCCCCE
TALDMAFFVKSYIEKFKFMLNIHSLKYFVYPKSKNLGTTLSSKFLNLKQKNANLLISDYP
EEHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCC
YSDGLKTGYIKESGLNLVATAQKDGRRLIAVVLGVKKGINGLGEKMRALIAKDLFEYGFN
CCCCCCCCCEECCCCEEEEECCCCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
EYSKFPLIVKLKEKVYNGTVDTVALFSKEPFYYVLTKDEFDKLKISYTVDKLVAPLSGDM
HHCCCCEEEEEHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEHHHHHHCCCCCC
PVGRAMIFLENEKVGDVALFSSKVNKLGFWQGLYKSFINFF
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSICVIKKLLLTLFLIFFLLFDDLFAANLAEINELSKHAKSIVLMDFDTKRILYSKNPN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEECCCC
LVFPPASLTKIVTIYTALIEAEKRNIKLKSIVPISDSASYYNAPLNSSLMFLEKGQIVNF
EEECCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCEECH
EEILKGLSVSSGNDASIAIAEFVVGDLNSFVNLMNINVLNLGLFNMHFVEPSGYSNENKI
HHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCCCCCCCE
TALDMAFFVKSYIEKFKFMLNIHSLKYFVYPKSKNLGTTLSSKFLNLKQKNANLLISDYP
EEHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCC
YSDGLKTGYIKESGLNLVATAQKDGRRLIAVVLGVKKGINGLGEKMRALIAKDLFEYGFN
CCCCCCCCCEECCCCEEEEECCCCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
EYSKFPLIVKLKEKVYNGTVDTVALFSKEPFYYVLTKDEFDKLKISYTVDKLVAPLSGDM
HHCCCCEEEEEHHHHHCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEHHHHHHCCCCCC
PVGRAMIFLENEKVGDVALFSSKVNKLGFWQGLYKSFINFF
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8955390; 9097040; 9278503 [H]