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The map label for this gene is mscS [H]

Identifier: 51598709

GI number: 51598709

Start: 474840

End: 475682

Strand: Direct

Name: mscS [H]

Synonym: BG0462

Alternate gene names: 51598709

Gene position: 474840-475682 (Clockwise)

Preceding gene: 51598700

Following gene: 51598711

Centisome position: 52.51

GC content: 26.93

Gene sequence:

>843_bases
TTGTTTAAGGAGTTTTTTATATTTCAAGATTATTTTAATTATATTCTTGAGAGTGTGGTAGGTTATGGTTTAAAGGTTTC
GATTGCTATAGCGCTATGGTATTTTTTAAAGTTAATAGTTAAGAAAATAGGTAAAATTTTATTTAAGACTTTGGAAAAGT
CTAAATTAGAGGAGAAGTTAGAGGCTACGGTTTTTAACTTTTTAAAATCTTTTTTCAAAATCTTAACAGATTTTCTTATT
GTTTTAATAATATTGCCATATCTTGGGGTGCCTACAACATCTATTATTGCTGTATTTGGATCATTAGGGCTTGCTATTGG
GCTTGCTGCTCAGGGTATTTTATCTAATTTTGTTAGCGGAATTATTGTTTTGAATTCTAAGTTTTTTAAGTGTGGAGATC
ATATTAAATGTGGCGATGTTGAAGGTTTGGTTGAAAATGTCCAAATTTTTTTTACTACACTTAAAACATTCGACGAAGAA
ATTATTAAAATTCCAAACAGTAAGCTTACATCTAATTTTGTTGTTAATTTTTCAGCAAATCCTAGAAGAAGAGTTGTATT
TTCTTTTCAAGTTCCCCATGATACGAATATTGATTTATTGAAAGATAAAATAGAGGATTTAATAATTTTTAATAATAAGA
AATTTAATGTTGAGATTTGTTCTCCTACTCTTATTGTCAAAAAATATACTCCCTGTTACATAGTCGTGCAGGTTCGATTT
TTTATTAATACAGATGCTTTTTGGACTTTTCAATATTTTATTGGTGAGTCTATTAAAAATGTTTTATTGGATATGAAAAT
TAAGTTTCCAATTTGTTTTATACCTTTTGATAAGCTATATTAA

Upstream 100 bases:

>100_bases
TTACTTGTTGTTTTTACTAGATTTTTTCTATTGAAAAGGTTTGTTTGTTTTTGTGATATAATTCAATTAATTTATGTGAA
TATTAGAAGGAAAGTTTAGT

Downstream 100 bases:

>100_bases
TGATTTTTTCTTGCAATAATTTCTGAGTAATAATTTTCAATTTTTTTTGTAAAAAAATGGCTTGAAAATTTAATAGAGTG
TTTTTTAGCATTTTGTTTAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MFKEFFIFQDYFNYILESVVGYGLKVSIAIALWYFLKLIVKKIGKILFKTLEKSKLEEKLEATVFNFLKSFFKILTDFLI
VLIILPYLGVPTTSIIAVFGSLGLAIGLAAQGILSNFVSGIIVLNSKFFKCGDHIKCGDVEGLVENVQIFFTTLKTFDEE
IIKIPNSKLTSNFVVNFSANPRRRVVFSFQVPHDTNIDLLKDKIEDLIIFNNKKFNVEICSPTLIVKKYTPCYIVVQVRF
FINTDAFWTFQYFIGESIKNVLLDMKIKFPICFIPFDKLY

Sequences:

>Translated_280_residues
MFKEFFIFQDYFNYILESVVGYGLKVSIAIALWYFLKLIVKKIGKILFKTLEKSKLEEKLEATVFNFLKSFFKILTDFLI
VLIILPYLGVPTTSIIAVFGSLGLAIGLAAQGILSNFVSGIIVLNSKFFKCGDHIKCGDVEGLVENVQIFFTTLKTFDEE
IIKIPNSKLTSNFVVNFSANPRRRVVFSFQVPHDTNIDLLKDKIEDLIIFNNKKFNVEICSPTLIVKKYTPCYIVVQVRF
FINTDAFWTFQYFIGESIKNVLLDMKIKFPICFIPFDKLY
>Mature_280_residues
MFKEFFIFQDYFNYILESVVGYGLKVSIAIALWYFLKLIVKKIGKILFKTLEKSKLEEKLEATVFNFLKSFFKILTDFLI
VLIILPYLGVPTTSIIAVFGSLGLAIGLAAQGILSNFVSGIIVLNSKFFKCGDHIKCGDVEGLVENVQIFFTTLKTFDEE
IIKIPNSKLTSNFVVNFSANPRRRVVFSFQVPHDTNIDLLKDKIEDLIIFNNKKFNVEICSPTLIVKKYTPCYIVVQVRF
FINTDAFWTFQYFIGESIKNVLLDMKIKFPICFIPFDKLY

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=233, Percent_Identity=31.3304721030043, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1787591, Length=151, Percent_Identity=28.476821192053, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367355, Length=153, Percent_Identity=24.8366013071895, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 32234; Mature: 32234

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKEFFIFQDYFNYILESVVGYGLKVSIAIALWYFLKLIVKKIGKILFKTLEKSKLEEKL
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EATVFNFLKSFFKILTDFLIVLIILPYLGVPTTSIIAVFGSLGLAIGLAAQGILSNFVSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIVLNSKFFKCGDHIKCGDVEGLVENVQIFFTTLKTFDEEIIKIPNSKLTSNFVVNFSAN
EEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
PRRRVVFSFQVPHDTNIDLLKDKIEDLIIFNNKKFNVEICSPTLIVKKYTPCYIVVQVRF
CCEEEEEEEECCCCCCHHHHHHHHHHEEEECCCEEEEEEECCEEEEEECCCEEEEEEEEE
FINTDAFWTFQYFIGESIKNVLLDMKIKFPICFIPFDKLY
EECCCCCEEHHHHHHHHHHHHHHHEEEECCEEEECHHHCC
>Mature Secondary Structure
MFKEFFIFQDYFNYILESVVGYGLKVSIAIALWYFLKLIVKKIGKILFKTLEKSKLEEKL
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EATVFNFLKSFFKILTDFLIVLIILPYLGVPTTSIIAVFGSLGLAIGLAAQGILSNFVSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIVLNSKFFKCGDHIKCGDVEGLVENVQIFFTTLKTFDEEIIKIPNSKLTSNFVVNFSAN
EEEECCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
PRRRVVFSFQVPHDTNIDLLKDKIEDLIIFNNKKFNVEICSPTLIVKKYTPCYIVVQVRF
CCEEEEEEEECCCCCCHHHHHHHHHHEEEECCCEEEEEEECCEEEEEECCCEEEEEEEEE
FINTDAFWTFQYFIGESIKNVLLDMKIKFPICFIPFDKLY
EECCCCCEEHHHHHHHHHHHHHHHEEEECCEEEECHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]