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The map label for this gene is capI [H]

Identifier: 51598699

GI number: 51598699

Start: 465031

End: 466098

Strand: Direct

Name: capI [H]

Synonym: BG0452

Alternate gene names: 51598699

Gene position: 465031-466098 (Clockwise)

Preceding gene: 51598698

Following gene: 51598700

Centisome position: 51.43

GC content: 29.59

Gene sequence:

>1068_bases
ATGAAAATTTTTTTAACCGGAATTGCAGGGTTTATTGGATTTCATGTAGCTAAAAAGCTTGTAGAAGAAGGACATGAAGT
TTTAGGTGTAGACATATTAAATGATTATTATGAACTTAAATTTAAAAATGAAAGATTAGAGGCTTTAGGTTTTTGTCCCA
AGGATGTCAAAACTCATAAGATTATTAAGAGTAAAAAATATAATAATTTATCCTTTGCCTACCTTGATATACTAAATAAG
GACAAACTACTGAAACTTTTTAAAGAGTATAAGTTTACACATGTTTGTCATTTGGCTGCTCAAGCGGGCATTAGAGATAG
TCTTGAAAATCCTGATAGCTATGTTTCAATAAATATTGTTGGATTTTTTAATGTTTTAGATGTATGTAGGGTTTATAAAG
AAAACATCAAACATTTTGTTTATGCTTCAACGTCATCAGTTTATGGTATAAATGAGAATATTCCATCTAGTGAGGATTCT
ATTACGGATCATCCTTTAAATTTATATGCAGCTAGTAAAAAATCTAATGAGATGATAGCTCATGCTTATAGTGCATCTTT
TAATATTCCTACAACAGGGCTTAGATTTTTTACAGTTTATGGAACATATGGAAGACCTGACATGGCTTTATATTTATTTT
CAGATGGAATTAAAAATTGTGAGCCTATTAATATTTTTAACAATGGGAATATGGCTAGAGATTTTACATATGTAAGTGAT
ATTGCAAACGGCGTTTACAAAGTGTTGAAAAATCCAGCTAAGAGTGATTGTAATTTTGACGTTAAAAATCCAAATTCGTC
AACATCTTCTTTTCCTTACAGAATATATAATATAGGGACTGGACATGCAACTAAATTGTTAGATTTTATTAGAGAGCTTG
AGGCTAATTTTGATAAAAAGGCCTTAAAAAATTATATGCCCATGCAAAAAGCAGATGTTGTGGAAAGCTGTTGTGATATT
GTAAAGCTTAAAAATGATGTGGGATATGAGGCTAAAATTTCTATTAAAGAAGGAATAAAAGAATTTTCACAGTGGTATAA
AATGTTAGAGTCTACTGAGAAGGTTTAA

Upstream 100 bases:

>100_bases
TAGTAACAATAATTCTCGCAGTTATCAAAAAAAAGATCTTGGTTTTAAAAAATAAATTGATGGCGTTAGTTTGTATTGAA
TTTTAAAGGGGAATGATAAT

Downstream 100 bases:

>100_bases
TTTTAAGCTTTTTGATACAGCATAATAAATTTTATGTTTATTTTTAGTTTAATAATTAATTATTTATTTAAATTCAATTC
TATTTCTTAGGTGTATAATT

Product: nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MKIFLTGIAGFIGFHVAKKLVEEGHEVLGVDILNDYYELKFKNERLEALGFCPKDVKTHKIIKSKKYNNLSFAYLDILNK
DKLLKLFKEYKFTHVCHLAAQAGIRDSLENPDSYVSINIVGFFNVLDVCRVYKENIKHFVYASTSSVYGINENIPSSEDS
ITDHPLNLYAASKKSNEMIAHAYSASFNIPTTGLRFFTVYGTYGRPDMALYLFSDGIKNCEPINIFNNGNMARDFTYVSD
IANGVYKVLKNPAKSDCNFDVKNPNSSTSSFPYRIYNIGTGHATKLLDFIRELEANFDKKALKNYMPMQKADVVESCCDI
VKLKNDVGYEAKISIKEGIKEFSQWYKMLESTEKV

Sequences:

>Translated_355_residues
MKIFLTGIAGFIGFHVAKKLVEEGHEVLGVDILNDYYELKFKNERLEALGFCPKDVKTHKIIKSKKYNNLSFAYLDILNK
DKLLKLFKEYKFTHVCHLAAQAGIRDSLENPDSYVSINIVGFFNVLDVCRVYKENIKHFVYASTSSVYGINENIPSSEDS
ITDHPLNLYAASKKSNEMIAHAYSASFNIPTTGLRFFTVYGTYGRPDMALYLFSDGIKNCEPINIFNNGNMARDFTYVSD
IANGVYKVLKNPAKSDCNFDVKNPNSSTSSFPYRIYNIGTGHATKLLDFIRELEANFDKKALKNYMPMQKADVVESCCDI
VKLKNDVGYEAKISIKEGIKEFSQWYKMLESTEKV
>Mature_355_residues
MKIFLTGIAGFIGFHVAKKLVEEGHEVLGVDILNDYYELKFKNERLEALGFCPKDVKTHKIIKSKKYNNLSFAYLDILNK
DKLLKLFKEYKFTHVCHLAAQAGIRDSLENPDSYVSINIVGFFNVLDVCRVYKENIKHFVYASTSSVYGINENIPSSEDS
ITDHPLNLYAASKKSNEMIAHAYSASFNIPTTGLRFFTVYGTYGRPDMALYLFSDGIKNCEPINIFNNGNMARDFTYVSD
IANGVYKVLKNPAKSDCNFDVKNPNSSTSSFPYRIYNIGTGHATKLLDFIRELEANFDKKALKNYMPMQKADVVESCCDI
VKLKNDVGYEAKISIKEGIKEFSQWYKMLESTEKV

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI56237023, Length=364, Percent_Identity=24.7252747252747, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI56118217, Length=364, Percent_Identity=24.7252747252747, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI189083684, Length=364, Percent_Identity=24.7252747252747, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI7657641, Length=353, Percent_Identity=26.0623229461756, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI42516563, Length=358, Percent_Identity=25.9776536312849, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI48994969, Length=376, Percent_Identity=23.6702127659574, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788353, Length=374, Percent_Identity=25.1336898395722, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786974, Length=363, Percent_Identity=21.4876033057851, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=372, Percent_Identity=25.8064516129032, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=372, Percent_Identity=25.8064516129032, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17539532, Length=357, Percent_Identity=24.6498599439776, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI133901786, Length=365, Percent_Identity=24.9315068493151, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=365, Percent_Identity=25.2054794520548, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=365, Percent_Identity=25.2054794520548, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=365, Percent_Identity=25.2054794520548, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=364, Percent_Identity=24.7252747252747, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115532424, Length=275, Percent_Identity=24.3636363636364, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=359, Percent_Identity=24.2339832869081, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=370, Percent_Identity=23.5135135135135, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19923002, Length=361, Percent_Identity=23.2686980609418, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21356223, Length=357, Percent_Identity=24.0896358543417, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 40343; Mature: 40343

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFLTGIAGFIGFHVAKKLVEEGHEVLGVDILNDYYELKFKNERLEALGFCPKDVKTHK
CEEEEEHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHEEECCCHHHHHCCCCCCHHHHH
IIKSKKYNNLSFAYLDILNKDKLLKLFKEYKFTHVCHLAAQAGIRDSLENPDSYVSINIV
HHHHCCCCCCEEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEE
GFFNVLDVCRVYKENIKHFVYASTSSVYGINENIPSSEDSITDHPLNLYAASKKSNEMIA
EHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCEE
HAYSASFNIPTTGLRFFTVYGTYGRPDMALYLFSDGIKNCEPINIFNNGNMARDFTYVSD
EEECCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHH
IANGVYKVLKNPAKSDCNFDVKNPNSSTSSFPYRIYNIGTGHATKLLDFIRELEANFDKK
HHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHH
ALKNYMPMQKADVVESCCDIVKLKNDVGYEAKISIKEGIKEFSQWYKMLESTEKV
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIFLTGIAGFIGFHVAKKLVEEGHEVLGVDILNDYYELKFKNERLEALGFCPKDVKTHK
CEEEEEHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHEEECCCHHHHHCCCCCCHHHHH
IIKSKKYNNLSFAYLDILNKDKLLKLFKEYKFTHVCHLAAQAGIRDSLENPDSYVSINIV
HHHHCCCCCCEEEEEEHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEE
GFFNVLDVCRVYKENIKHFVYASTSSVYGINENIPSSEDSITDHPLNLYAASKKSNEMIA
EHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCEE
HAYSASFNIPTTGLRFFTVYGTYGRPDMALYLFSDGIKNCEPINIFNNGNMARDFTYVSD
EEECCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHH
IANGVYKVLKNPAKSDCNFDVKNPNSSTSSFPYRIYNIGTGHATKLLDFIRELEANFDKK
HHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHH
ALKNYMPMQKADVVESCCDIVKLKNDVGYEAKISIKEGIKEFSQWYKMLESTEKV
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]