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The map label for this gene is ascG [H]

Identifier: 51598026

GI number: 51598026

Start: 4432454

End: 4433440

Strand: Direct

Name: ascG [H]

Synonym: YPTB3736

Alternate gene names: 51598026

Gene position: 4432454-4433440 (Clockwise)

Preceding gene: 51598025

Following gene: 51598028

Centisome position: 93.42

GC content: 51.77

Gene sequence:

>987_bases
ATGGTTACACTGGAAGACGTCGCGGTATTAGCCGGGGTATCCCGTGCTACGGTTTCCCGCGTGGTCAACGGTGATACCAA
TGTAAAGGCCCTTACACGTGAAAAGGTTGAGCAGGCTGTCGCCGTTCTCGGTTATACCCCGCACCCTGCCGCACGGGCAC
TGGCATCCAGCCAAAGTAATACGCTGGGGTTGGTGACCACCTCATACCGAGGGGGCTTTTTTGGTGCCTTAATGGATTTT
GTGCAAACAGAAACAGAATCCCAAGGTAAACAACTGTTGGTCACTCAGGGACGAGATAATGCAGATAAAGAATGGTTAGC
CATTCAGCGGCTGTATAACTTACGCTGCGATGGGTTAATCCTGCATGTGCGCTTTCTCAGCGACGAAAGGCTGCGTCAGT
TAGCTGCAGCAAACCGGCCTTTTGTATTATTGGATAGGTTGGTTCCTGATCTTGAGGCTCGCTGTGTGACCTTTGATCAC
CATCATGCCAGCCAAATGGCAACGCAGGTGTTGATTGATGCGGGCCATCGCGACATCGCCTGTATCAGCGGCTCTTCACA
ACGCCAATCCAGTGTTTTACGCATTCAGGGCTTTCTTGATGCCATGCAGTTGGCGGGCTTAGAACCCGTGGCTTGTTTGG
AGGGCGTTTACGATCTGGAAAGTGGCTACCTGCGGGCTGACGAAATCCTCAAGCAACCCCGGCTACCGACGGCTATCTAT
TGCTGTAATGAAGAGATGGCCATTGGAGCATTACTGGCGATAAATAAACACGGTTTACAGGTGCCGCAAGATATTTCGCT
GATTTGCTATGACAGTGGCGAGCGCGCTCCTTTTGTCTGCCCAGCCTTGACCAGCGTACACTTTCCAATCGTGGAAATGG
CGCAATATGCTACACAGTTGCTGATTGACCCGCTGATCCCTGCCGTCAATTTCCCACCCGCGATTATCATGCGTGATTCT
GTTATGCCGCCGAAAACAGCGTGTTAA

Upstream 100 bases:

>100_bases
AGGCTGCGAATACCGCAGCAGCGTCAAGGACGAAAGGTATAAACTAACGATTATGGCGGCCATATATAGGGTCGCCGTTT
CACCTTAACAGGAGCAAAAG

Downstream 100 bases:

>100_bases
TTGGTGTTGGTATTGGTATTAATTGTTATTGGTATTAATTGTTATTGGTATTAATTGTTATTAACGTGGCAAGCCATGGC
CTGCCACGTTCTGCTAGCGC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MVTLEDVAVLAGVSRATVSRVVNGDTNVKALTREKVEQAVAVLGYTPHPAARALASSQSNTLGLVTTSYRGGFFGALMDF
VQTETESQGKQLLVTQGRDNADKEWLAIQRLYNLRCDGLILHVRFLSDERLRQLAAANRPFVLLDRLVPDLEARCVTFDH
HHASQMATQVLIDAGHRDIACISGSSQRQSSVLRIQGFLDAMQLAGLEPVACLEGVYDLESGYLRADEILKQPRLPTAIY
CCNEEMAIGALLAINKHGLQVPQDISLICYDSGERAPFVCPALTSVHFPIVEMAQYATQLLIDPLIPAVNFPPAIIMRDS
VMPPKTAC

Sequences:

>Translated_328_residues
MVTLEDVAVLAGVSRATVSRVVNGDTNVKALTREKVEQAVAVLGYTPHPAARALASSQSNTLGLVTTSYRGGFFGALMDF
VQTETESQGKQLLVTQGRDNADKEWLAIQRLYNLRCDGLILHVRFLSDERLRQLAAANRPFVLLDRLVPDLEARCVTFDH
HHASQMATQVLIDAGHRDIACISGSSQRQSSVLRIQGFLDAMQLAGLEPVACLEGVYDLESGYLRADEILKQPRLPTAIY
CCNEEMAIGALLAINKHGLQVPQDISLICYDSGERAPFVCPALTSVHFPIVEMAQYATQLLIDPLIPAVNFPPAIIMRDS
VMPPKTAC
>Mature_328_residues
MVTLEDVAVLAGVSRATVSRVVNGDTNVKALTREKVEQAVAVLGYTPHPAARALASSQSNTLGLVTTSYRGGFFGALMDF
VQTETESQGKQLLVTQGRDNADKEWLAIQRLYNLRCDGLILHVRFLSDERLRQLAAANRPFVLLDRLVPDLEARCVTFDH
HHASQMATQVLIDAGHRDIACISGSSQRQSSVLRIQGFLDAMQLAGLEPVACLEGVYDLESGYLRADEILKQPRLPTAIY
CCNEEMAIGALLAINKHGLQVPQDISLICYDSGERAPFVCPALTSVHFPIVEMAQYATQLLIDPLIPAVNFPPAIIMRDS
VMPPKTAC

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=36.5269461077844, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1788474, Length=337, Percent_Identity=36.2017804154303, Blast_Score=190, Evalue=9e-50,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=36.036036036036, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=32.6283987915408, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1790194, Length=307, Percent_Identity=33.5504885993485, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1790369, Length=307, Percent_Identity=30.2931596091205, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1786540, Length=332, Percent_Identity=30.421686746988, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI48994940, Length=304, Percent_Identity=27.3026315789474, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=27.6527331189711, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787580, Length=309, Percent_Identity=25.5663430420712, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787906, Length=264, Percent_Identity=28.4090909090909, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786268, Length=288, Percent_Identity=23.6111111111111, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790689, Length=257, Percent_Identity=27.2373540856031, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35841; Mature: 35841

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTLEDVAVLAGVSRATVSRVVNGDTNVKALTREKVEQAVAVLGYTPHPAARALASSQSN
CCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
TLGLVTTSYRGGFFGALMDFVQTETESQGKQLLVTQGRDNADKEWLAIQRLYNLRCDGLI
EEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEE
LHVRFLSDERLRQLAAANRPFVLLDRLVPDLEARCVTFDHHHASQMATQVLIDAGHRDIA
EEEEECCHHHHHHHHHCCCCEEEHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEE
CISGSSQRQSSVLRIQGFLDAMQLAGLEPVACLEGVYDLESGYLRADEILKQPRLPTAIY
EECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEE
CCNEEMAIGALLAINKHGLQVPQDISLICYDSGERAPFVCPALTSVHFPIVEMAQYATQL
EECCCHHHHEEEEECCCCCCCCCCCEEEEECCCCCCCEECHHHHHCCCHHHHHHHHHHHH
LIDPLIPAVNFPPAIIMRDSVMPPKTAC
HHHHHCCCCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MVTLEDVAVLAGVSRATVSRVVNGDTNVKALTREKVEQAVAVLGYTPHPAARALASSQSN
CCCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
TLGLVTTSYRGGFFGALMDFVQTETESQGKQLLVTQGRDNADKEWLAIQRLYNLRCDGLI
EEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEE
LHVRFLSDERLRQLAAANRPFVLLDRLVPDLEARCVTFDHHHASQMATQVLIDAGHRDIA
EEEEECCHHHHHHHHHCCCCEEEHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEE
CISGSSQRQSSVLRIQGFLDAMQLAGLEPVACLEGVYDLESGYLRADEILKQPRLPTAIY
EECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEE
CCNEEMAIGALLAINKHGLQVPQDISLICYDSGERAPFVCPALTSVHFPIVEMAQYATQL
EECCCHHHHEEEEECCCCCCCCCCCEEEEECCCCCCCEECHHHHHCCCHHHHHHHHHHHH
LIDPLIPAVNFPPAIIMRDSVMPPKTAC
HHHHHCCCCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]