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The map label for this gene is cysJ

Identifier: 51595109

GI number: 51595109

Start: 914272

End: 916128

Strand: Direct

Name: cysJ

Synonym: YPTB0759

Alternate gene names: 51595109

Gene position: 914272-916128 (Clockwise)

Preceding gene: 51595107

Following gene: 51595110

Centisome position: 19.27

GC content: 52.07

Gene sequence:

>1857_bases
ATGACGACTCAGGCTCCTCCAACTTCGTTACTTCCGCTATCGCCAGAGCAGTTGGCCCGCTTGCAAGCGGCGGTGGGTGA
ATTCTCCCCAACCCAAATGGCATGGTTGTCGGGTTACTTCTGGGGGATGGTTAACCAGCAGCCCGGCGCTGTTGCTTCCC
CTGCGGTAGCGGCACCCCCACCAGTGACAGTTACCCTGATTTCCGCGTCTCAAACCGGCAACGCCCGGCGTTTAGCTGAA
CAACTGCGCGATGATCTTCTGGCTGCCCAACTTAGTGTCAATCTGGTCAATGCGGGTGATTATAAGTTTAAACAAATTGC
GCAGGAGCGTTTACTGGTGGTGGTCGCATCGACCCAAGGAGAGGGGGAGCCAGCAGAAGAAGCCGTCGCACTGCATAAAT
TCCTGTTTTCTAAAAAAGCCCCAAAATTATCGGAGACCGCATTTGCTGTCTTTGGCTTAGGTGACACCTCTTACGAACAC
TTCTGTCAGGCTGGGAAAGATTTTGATAGCAAGCTGGCAGAACTTGGTGCTCAACGGTTACTGGATCGTGTCGATGCCGA
TGTTGAATATCAGGTACAAGCACAGCAGTGGCGTCAGCAAGTGGTTGCCACGTTGCAGGCTAAGGTTCCAGCACAATCTA
CAGCTCCAACACAATTTATAGCTCCAACACAATTTATAGCTCCAACACAATCTACAACTCCAGCAGCAGCGGCGATAACG
TCTGGTGGTACCACTACCGTTAGTCCGTACAGTAAAACCGCGCCGTTGACCGCACAGTTATCCGTACAGCAAAAAGTGAC
TGGGCGTAATTCAGAAAAAGATGTTCGCCATATTGAAATCGATTTAGGTGATTCAGGACTGCGTTACCAACCAGGCGATG
CTTTGGGTGTCTGGTTTGATAATGATCCTGCTTTGGTCGAAGAACTGCTCGCGCTGTTGTGGCTCAAAGGTGATGAGCCG
GTCAGTATTGACGGACAAAATATGCCACTGGCACAGGCGCTGCTCAGTCACCTTGAACTGACACAAAATACGACGCTGAT
CGTCGATAAATACGCCGCGCTATCACGCGATGAAACCTTGATTGCACTGCTGGCAGATAAACCCGCGTTGCAACTGTACG
CTAAGAATACGCCATTCGTTGATATGGTGCGGCAGGCACCGTCTGATCTTAATGCCGATCAACTGGTGGGGTTATTGCGC
CCCTTAACGCCACGGTTGTACTCCATCGCCTCTTCGCAAGCTGAGACCGAAAATGAGGTGCATATCACCGTGGGTGTGGT
GCGCTATGACATTGATGGCCGAGCGCGCAGTGGCGGTGCTTCCGGCTATCTGGCGGACCGGCTGGAAGTCGATGGCGATA
TCCGTGTCTTTATTGAACACAATGATAATTTCCGTTTGCCTGCTAACCCGGAAACACCCGTCATCATGATCGGGCCAGGC
ACCGGTATCGCGCCGTTCCGCGCCTTTATGCAACAGCGTGAAGTTGATGGGGCCAGTGGCAAGAACTGGTTATTCTTCGG
TAATCCTCATTTCACCGAGGATTTTCTGTATCAGGTTGAATGGCAACGCTATGTCAAAGAAGGCGTGTTGACGCGCATTG
ATCTGGCCTGGTCCCGTGATCAGGCGCATAAAATATACGTACAAGACAAACTGCGCGAACAGGGCGCGGAACTGTGGAAC
TGGATACAACAAGGTGCCCACATTTACGTCTGTGGCGATGCTAACCGCATGGCGAAAGACGTTGAGCAGGTTTTACTGGA
CGTGGTGGCTCTGCACGGTGCAATGGATGCCGAGCAGGCTGATGAATATTTAAGTGAGTTGCGCCAAGCGCGCCGCTATC
AGCGAGATGTGTACTGA

Upstream 100 bases:

>100_bases
ACCTTACAAACTATCAAGACTTTGTGGCACGAATTGTCCTGCCTATATTGACAAATATTTGTGCCAACCACGGCTAAAGC
ATAAGGGAATTCGTACTGCA

Downstream 100 bases:

>100_bases
TGAATGAAAAACACCCAGGGCCACTCGTCGTCAGTGGAAAACTTTCTGATGGCGAGCGCATGAAGTCGGAAAGCAATTTC
TTGCGTGGGACTATTGCGGA

Product: sulfite reductase subunit alpha

Products: NA

Alternate protein names: SiR-FP

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MTTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPPPVTVTLISASQTGNARRLAE
QLRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQGEGEPAEEAVALHKFLFSKKAPKLSETAFAVFGLGDTSYEH
FCQAGKDFDSKLAELGAQRLLDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTAPTQFIAPTQFIAPTQSTTPAAAAIT
SGGTTTVSPYSKTAPLTAQLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFDNDPALVEELLALLWLKGDEP
VSIDGQNMPLAQALLSHLELTQNTTLIVDKYAALSRDETLIALLADKPALQLYAKNTPFVDMVRQAPSDLNADQLVGLLR
PLTPRLYSIASSQAETENEVHITVGVVRYDIDGRARSGGASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPG
TGIAPFRAFMQQREVDGASGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQAHKIYVQDKLREQGAELWN
WIQQGAHIYVCGDANRMAKDVEQVLLDVVALHGAMDAEQADEYLSELRQARRYQRDVY

Sequences:

>Translated_618_residues
MTTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPPPVTVTLISASQTGNARRLAE
QLRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQGEGEPAEEAVALHKFLFSKKAPKLSETAFAVFGLGDTSYEH
FCQAGKDFDSKLAELGAQRLLDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTAPTQFIAPTQFIAPTQSTTPAAAAIT
SGGTTTVSPYSKTAPLTAQLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFDNDPALVEELLALLWLKGDEP
VSIDGQNMPLAQALLSHLELTQNTTLIVDKYAALSRDETLIALLADKPALQLYAKNTPFVDMVRQAPSDLNADQLVGLLR
PLTPRLYSIASSQAETENEVHITVGVVRYDIDGRARSGGASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPG
TGIAPFRAFMQQREVDGASGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQAHKIYVQDKLREQGAELWN
WIQQGAHIYVCGDANRMAKDVEQVLLDVVALHGAMDAEQADEYLSELRQARRYQRDVY
>Mature_617_residues
TTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPPPVTVTLISASQTGNARRLAEQ
LRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQGEGEPAEEAVALHKFLFSKKAPKLSETAFAVFGLGDTSYEHF
CQAGKDFDSKLAELGAQRLLDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTAPTQFIAPTQFIAPTQSTTPAAAAITS
GGTTTVSPYSKTAPLTAQLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFDNDPALVEELLALLWLKGDEPV
SIDGQNMPLAQALLSHLELTQNTTLIVDKYAALSRDETLIALLADKPALQLYAKNTPFVDMVRQAPSDLNADQLVGLLRP
LTPRLYSIASSQAETENEVHITVGVVRYDIDGRARSGGASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPGT
GIAPFRAFMQQREVDGASGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQAHKIYVQDKLREQGAELWNW
IQQGAHIYVCGDANRMAKDVEQVLLDVVALHGAMDAEQADEYLSELRQARRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain

Homologues:

Organism=Homo sapiens, GI127139033, Length=605, Percent_Identity=31.2396694214876, Blast_Score=258, Evalue=2e-68,
Organism=Homo sapiens, GI24041029, Length=598, Percent_Identity=31.9397993311037, Blast_Score=236, Evalue=5e-62,
Organism=Homo sapiens, GI7657393, Length=599, Percent_Identity=29.8831385642738, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI221316709, Length=599, Percent_Identity=29.8831385642738, Blast_Score=212, Evalue=8e-55,
Organism=Homo sapiens, GI221316705, Length=608, Percent_Identity=29.4407894736842, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI10835173, Length=652, Percent_Identity=28.9877300613497, Blast_Score=195, Evalue=9e-50,
Organism=Homo sapiens, GI221316707, Length=545, Percent_Identity=29.1743119266055, Blast_Score=180, Evalue=4e-45,
Organism=Homo sapiens, GI40254422, Length=650, Percent_Identity=27.8461538461538, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI169790958, Length=446, Percent_Identity=27.8026905829596, Blast_Score=155, Evalue=2e-37,
Organism=Homo sapiens, GI169790956, Length=446, Percent_Identity=27.8026905829596, Blast_Score=155, Evalue=2e-37,
Organism=Escherichia coli, GI1789123, Length=618, Percent_Identity=72.4919093851133, Blast_Score=917, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554134, Length=644, Percent_Identity=29.3478260869565, Blast_Score=249, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17566446, Length=577, Percent_Identity=24.9566724436742, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17531441, Length=395, Percent_Identity=26.5822784810127, Blast_Score=99, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321832, Length=639, Percent_Identity=28.0125195618153, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6321143, Length=394, Percent_Identity=31.7258883248731, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325305, Length=627, Percent_Identity=23.2854864433812, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17137192, Length=601, Percent_Identity=28.7853577371048, Blast_Score=223, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24582192, Length=511, Percent_Identity=30.1369863013699, Blast_Score=202, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24583543, Length=529, Percent_Identity=29.4896030245747, Blast_Score=185, Evalue=9e-47,
Organism=Drosophila melanogaster, GI78706876, Length=529, Percent_Identity=29.4896030245747, Blast_Score=185, Evalue=9e-47,
Organism=Drosophila melanogaster, GI78706872, Length=529, Percent_Identity=29.4896030245747, Blast_Score=185, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24660907, Length=604, Percent_Identity=25.8278145695364, Blast_Score=139, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24660903, Length=604, Percent_Identity=25.8278145695364, Blast_Score=139, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSJ_YERPS (Q66ED4)

Other databases:

- EMBL:   BX936398
- RefSeq:   YP_069300.1
- ProteinModelPortal:   Q66ED4
- SMR:   Q66ED4
- GeneID:   2955407
- GenomeReviews:   BX936398_GR
- KEGG:   yps:YPTB0759
- NMPDR:   fig|273123.1.peg.884
- HOGENOM:   HBG736048
- OMA:   HVEIDLG
- ProtClustDB:   PRK10953
- BioCyc:   YPSE273123:YPTB0759-MONOMER
- BRENDA:   1.8.1.2
- GO:   GO:0006810
- HAMAP:   MF_01541
- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938
- Gene3D:   G3DSA:1.20.990.10
- PIRSF:   PIRSF000207
- PRINTS:   PR00369
- PRINTS:   PR00371
- TIGRFAMs:   TIGR01931

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1; SSF63380 Riboflavin_synthase_like_b-brl

EC number: =1.8.1.2

Molecular weight: Translated: 67941; Mature: 67810

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS51384 FAD_FR; PS50902 FLAVODOXIN_LIKE

Important sites: BINDING 508-508

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPP
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCC
PVTVTLISASQTGNARRLAEQLRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQG
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCEEEEEEECCC
EGEPAEEAVALHKFLFSKKAPKLSETAFAVFGLGDTSYEHFCQAGKDFDSKLAELGAQRL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHH
LDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTAPTQFIAPTQFIAPTQSTTPAAAAIT
HHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEECCCCCCCCCHHEEE
SGGTTTVSPYSKTAPLTAQLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFD
CCCCEEECCCCCCCCCEEEEEHHHHHCCCCCCCCCEEEEEEECCCCCEECCCCEEEEEEC
NDPALVEELLALLWLKGDEPVSIDGQNMPLAQALLSHLELTQNTTLIVDKYAALSRDETL
CCHHHHHHHHHHHEECCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECHHHCCCCCEE
IALLADKPALQLYAKNTPFVDMVRQAPSDLNADQLVGLLRPLTPRLYSIASSQAETENEV
EEEEECCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCE
HITVGVVRYDIDGRARSGGASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPG
EEEEEEEEEECCCCCCCCCCCCEEEHHEECCCCEEEEEEECCCEECCCCCCCCEEEEECC
TGIAPFRAFMQQREVDGASGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRD
CCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCC
QAHKIYVQDKLREQGAELWNWIQQGAHIYVCGDANRMAKDVEQVLLDVVALHGAMDAEQA
CCEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHH
DEYLSELRQARRYQRDVY
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPP
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCC
PVTVTLISASQTGNARRLAEQLRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQG
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCEEEEEEECCC
EGEPAEEAVALHKFLFSKKAPKLSETAFAVFGLGDTSYEHFCQAGKDFDSKLAELGAQRL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHH
LDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTAPTQFIAPTQFIAPTQSTTPAAAAIT
HHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCEECCCCCCCCCHHEEE
SGGTTTVSPYSKTAPLTAQLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFD
CCCCEEECCCCCCCCCEEEEEHHHHHCCCCCCCCCEEEEEEECCCCCEECCCCEEEEEEC
NDPALVEELLALLWLKGDEPVSIDGQNMPLAQALLSHLELTQNTTLIVDKYAALSRDETL
CCHHHHHHHHHHHEECCCCCEEECCCCCCHHHHHHHHHHHCCCCEEEEECHHHCCCCCEE
IALLADKPALQLYAKNTPFVDMVRQAPSDLNADQLVGLLRPLTPRLYSIASSQAETENEV
EEEEECCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCE
HITVGVVRYDIDGRARSGGASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPG
EEEEEEEEEECCCCCCCCCCCCEEEHHEECCCCEEEEEEECCCEECCCCCCCCEEEEECC
TGIAPFRAFMQQREVDGASGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRD
CCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCC
QAHKIYVQDKLREQGAELWNWIQQGAHIYVCGDANRMAKDVEQVLLDVVALHGAMDAEQA
CCEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHH
DEYLSELRQARRYQRDVY
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA