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The map label for this gene is prfC

Identifier: 51594926

GI number: 51594926

Start: 677036

End: 678625

Strand: Direct

Name: prfC

Synonym: YPTB0575

Alternate gene names: 51594926

Gene position: 677036-678625 (Clockwise)

Preceding gene: 51594925

Following gene: 51594927

Centisome position: 14.27

GC content: 49.56

Gene sequence:

>1590_bases
ATGTCTCCTAGTGAATATGCTCTGGAAGTCGCGAAAAGACGTACTTTTGCGATCATTTCCCACCCCGATGCCGGTAAAAC
AACCATTACCGAAAAAGTGTTGCTGTTCGGACATGCAATTCAGACTGCCGGGACAGTAAAAGGCCGTGGTTCAAGCCATC
ATGCCAAATCTGACTGGATGGAAATGGAAAAACAACGCGGTATCTCCATTACCACGTCGGTAATGCAGTTTCCGTATGGC
GGCTGTCTGGTCAATCTGCTTGATACCCCAGGGCATGAAGACTTCTCCGAAGATACGTACCGCACACTGACGGCGGTTGA
CTGCTGTTTGATGGTGATCGATGCGGCCAAAGGGGTAGAAGACCGGACCCGCAAATTGATGGAAGTGACCCGTCTGCGCG
ATACGCCAATTCTGACCTTTATGAACAAATTGGACCGCGAAATTCGTGATCCAATGGAAGTTCTGGATGAAGTCGAGCGT
GAGCTGAACATTGCCTGTTCACCTATCACTTGGCCGATTGGTTGCGGTAAGTCGTTTAAAGGGGTTTATCACCTTCATAA
AGACGAAACTTATCTGTATCAAAGCGGTAAAGGCCATACCATTCAGGAAGTCCGTATCGTTAAAGGGCTGAACAACCCAG
ATCTGGATGTGGCTGTTGGCGAAGACTTGGCCAAGCAGTTCCGTCAGGAGTTGGAACTGGTTCAGGGCGCTTCCCACGAG
TTTGATCATGAGGCTTTCCTGTCCGGTGATTTAACGCCCGTCTTCTTCGGTACTGCGTTGGGTAACTTCGGTGTGGACCA
TATGCTAGATGGCTTGGTCGAATGGGCACCGGCACCGATGCCGCGTAAAACGGATACCCGCGTAGTGGTCGCCTCTGAAG
AGAAATTCACCGGTTTCGTTTTCAAAATTCAGGCCAATATGGATCCGAAACACCGTGACCGTGTGGCCTTTATGCGCGTG
GTGTCTGGCCGTTTTGAGAAAGGGATGAAGCTGCGTCAGGTACGGACCAAGAAAGACGTGGTGATTTCAGACGCGCTGAC
CTTTATGGCCGGTGACCGTTCTCACGTTGAAGAGGCCTATGCTGGTGATATTATCGGCTTGCATAACCATGGCACCATCC
AGATCGGTGACACCTTCACTCAAGGTGAAGATATGAAATTCACCGGTATTCCTAACTTTGCCCCTGAATTGTTCCGCCGC
ATTCGCCTGCGCGATCCATTAAAGCAGAAGCAGTTGTTAAAAGGCTTGGTTCAGTTGTCAGAAGAGGGAGCCGTTCAGGT
ATTCCGCCCACTGAGCAACAACGATTTGATCGTGGGCGCTGTGGGGGTGCTGCAGTTTGAAGTGGTCTCTTCCCGCTTGA
AGAGTGAATACAACGTCGAAGCGGTGTATGAATCAGTTAACGTTTCTACCGCGCGTTGGGTCGAATGTAATGACGTGAAG
AAATTTGAGGAGTTCAAACGTAAGAATGAGCTTAATTTGGCCTTAGACGGTGGTGATAATTTAAGTTACATCGCGCCCAC
GATGGTTAATCTCAATATTACACAAGAGCGTTACCCGGACGTTATTTTCCGCAAAACACGCGAGCACTAA

Upstream 100 bases:

>100_bases
GAAAATATCGCGCAATAATCAATGTCTCGCTTTGCCGGACCCACATAGCAATAAAATGTGAGGCTCAGAGCAGGGCGGCC
TAAAAGTAGAAACTTCAACT

Downstream 100 bases:

>100_bases
GTTTATTGCTAACGAACCTGCTGCAAGCCGCATTCTATCGGGGTATCTATTACCCCGATCCATCAATCTTATTCGATATT
TTCTTGCTAGGATAATTCCT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSPSEYALEVAKRRTFAIISHPDAGKTTITEKVLLFGHAIQTAGTVKGRGSSHHAKSDWMEMEKQRGISITTSVMQFPYG
GCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDREIRDPMEVLDEVER
ELNIACSPITWPIGCGKSFKGVYHLHKDETYLYQSGKGHTIQEVRIVKGLNNPDLDVAVGEDLAKQFRQELELVQGASHE
FDHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVEWAPAPMPRKTDTRVVVASEEKFTGFVFKIQANMDPKHRDRVAFMRV
VSGRFEKGMKLRQVRTKKDVVISDALTFMAGDRSHVEEAYAGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRR
IRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLSNNDLIVGAVGVLQFEVVSSRLKSEYNVEAVYESVNVSTARWVECNDVK
KFEEFKRKNELNLALDGGDNLSYIAPTMVNLNITQERYPDVIFRKTREH

Sequences:

>Translated_529_residues
MSPSEYALEVAKRRTFAIISHPDAGKTTITEKVLLFGHAIQTAGTVKGRGSSHHAKSDWMEMEKQRGISITTSVMQFPYG
GCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDREIRDPMEVLDEVER
ELNIACSPITWPIGCGKSFKGVYHLHKDETYLYQSGKGHTIQEVRIVKGLNNPDLDVAVGEDLAKQFRQELELVQGASHE
FDHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVEWAPAPMPRKTDTRVVVASEEKFTGFVFKIQANMDPKHRDRVAFMRV
VSGRFEKGMKLRQVRTKKDVVISDALTFMAGDRSHVEEAYAGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRR
IRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLSNNDLIVGAVGVLQFEVVSSRLKSEYNVEAVYESVNVSTARWVECNDVK
KFEEFKRKNELNLALDGGDNLSYIAPTMVNLNITQERYPDVIFRKTREH
>Mature_528_residues
SPSEYALEVAKRRTFAIISHPDAGKTTITEKVLLFGHAIQTAGTVKGRGSSHHAKSDWMEMEKQRGISITTSVMQFPYGG
CLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDREIRDPMEVLDEVERE
LNIACSPITWPIGCGKSFKGVYHLHKDETYLYQSGKGHTIQEVRIVKGLNNPDLDVAVGEDLAKQFRQELELVQGASHEF
DHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVEWAPAPMPRKTDTRVVVASEEKFTGFVFKIQANMDPKHRDRVAFMRVV
SGRFEKGMKLRQVRTKKDVVISDALTFMAGDRSHVEEAYAGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRRI
RLRDPLKQKQLLKGLVQLSEEGAVQVFRPLSNNDLIVGAVGVLQFEVVSSRLKSEYNVEAVYESVNVSTARWVECNDVKK
FEEFKRKNELNLALDGGDNLSYIAPTMVNLNITQERYPDVIFRKTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=481, Percent_Identity=27.8586278586279, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI19923640, Length=529, Percent_Identity=25.3308128544423, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=26.9035532994924, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI25306287, Length=501, Percent_Identity=24.3512974051896, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI157426893, Length=169, Percent_Identity=28.9940828402367, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI94966754, Length=142, Percent_Identity=30.2816901408451, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1790835, Length=529, Percent_Identity=89.0359168241966, Blast_Score=987, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=493, Percent_Identity=25.1521298174442, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI48994988, Length=216, Percent_Identity=30.0925925925926, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788922, Length=151, Percent_Identity=33.112582781457, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=500, Percent_Identity=27, Blast_Score=171, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17556745, Length=179, Percent_Identity=31.2849162011173, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=134, Percent_Identity=29.8507462686567, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=485, Percent_Identity=27.2164948453608, Blast_Score=169, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=419, Percent_Identity=26.0143198090692, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=25.8278145695364, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24582462, Length=482, Percent_Identity=26.7634854771784, Blast_Score=169, Evalue=6e-42,
Organism=Drosophila melanogaster, GI221458488, Length=507, Percent_Identity=24.4575936883629, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=31.875, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24585709, Length=156, Percent_Identity=28.8461538461538, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585711, Length=156, Percent_Identity=28.8461538461538, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585713, Length=156, Percent_Identity=28.8461538461538, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_YERP3 (A7FMI1)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402459.1
- ProteinModelPortal:   A7FMI1
- SMR:   A7FMI1
- STRING:   A7FMI1
- GeneID:   5386979
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3504
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- ProtClustDB:   PRK00741
- BioCyc:   YPSE349747:YPSIP31758_3504-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59618; Mature: 59487

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSEYALEVAKRRTFAIISHPDAGKTTITEKVLLFGHAIQTAGTVKGRGSSHHAKSDWM
CCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
EMEKQRGISITTSVMQFPYGGCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVE
HHHHHCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
DRTRKLMEVTRLRDTPILTFMNKLDREIRDPMEVLDEVERELNIACSPITWPIGCGKSFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCEEECCCEECCCCCCCCC
GVYHLHKDETYLYQSGKGHTIQEVRIVKGLNNPDLDVAVGEDLAKQFRQELELVQGASHE
CEEEEECCCEEEEECCCCCCHHHHHEECCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCC
FDHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVEWAPAPMPRKTDTRVVVASEEKFTGFV
CCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEE
FKIQANMDPKHRDRVAFMRVVSGRFEKGMKLRQVRTKKDVVISDALTFMAGDRSHVEEAY
EEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEHHHHHHHHCCCHHHHHHHH
AGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRRIRLRDPLKQKQLLKGLVQLS
CCCEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
EEGAVQVFRPLSNNDLIVGAVGVLQFEVVSSRLKSEYNVEAVYESVNVSTARWVECNDVK
CCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEHHHHH
KFEEFKRKNELNLALDGGDNLSYIAPTMVNLNITQERYPDVIFRKTREH
HHHHHHCCCCCEEEEECCCCCEEECCEEEEEEECHHHCCHHHHHHCCCC
>Mature Secondary Structure 
SPSEYALEVAKRRTFAIISHPDAGKTTITEKVLLFGHAIQTAGTVKGRGSSHHAKSDWM
CCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
EMEKQRGISITTSVMQFPYGGCLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVE
HHHHHCCCEEEHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
DRTRKLMEVTRLRDTPILTFMNKLDREIRDPMEVLDEVERELNIACSPITWPIGCGKSFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCEEECCCEECCCCCCCCC
GVYHLHKDETYLYQSGKGHTIQEVRIVKGLNNPDLDVAVGEDLAKQFRQELELVQGASHE
CEEEEECCCEEEEECCCCCCHHHHHEECCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCC
FDHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVEWAPAPMPRKTDTRVVVASEEKFTGFV
CCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEE
FKIQANMDPKHRDRVAFMRVVSGRFEKGMKLRQVRTKKDVVISDALTFMAGDRSHVEEAY
EEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEHHHHHHHHCCCHHHHHHHH
AGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRRIRLRDPLKQKQLLKGLVQLS
CCCEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
EEGAVQVFRPLSNNDLIVGAVGVLQFEVVSSRLKSEYNVEAVYESVNVSTARWVECNDVK
CCCCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEHHHHH
KFEEFKRKNELNLALDGGDNLSYIAPTMVNLNITQERYPDVIFRKTREH
HHHHHHCCCCCEEEEECCCCCEEECCEEEEEEECHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA