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The map label for this gene is pstA

Identifier: 51594896

GI number: 51594896

Start: 642999

End: 645539

Strand: Direct

Name: pstA

Synonym: YPTB0545

Alternate gene names: 51594896

Gene position: 642999-645539 (Clockwise)

Preceding gene: 51594892

Following gene: 51594904

Centisome position: 13.55

GC content: 52.74

Gene sequence:

>2541_bases
ATGACCAAGCAAGTGACATTTATCTGCGGGCTTCCTAATGGCGTTCATGCCCGACCCGCGAGCCATATAGAAAGGGTGTG
CAACCGCTTCCAGTCGCGTTTCCACTGGTATAATCCGCGTTCTGGCATCGTGGGGGATGGCAAGAGCGTACTTTCGCTGA
TCGCGACCAATATCCTGTTAGGGGATGAATGTCAGGTCACCATTGAAGGCGAAGATGAGCAGGCTGCTTTTGAACGCCTG
AGTATTTTTATTGAGCACGAGCTTCCACACGCTGACCCCATCTTGCCCAAGAGGGAGGAGAGTGCAGAATGGGAGCCGCT
CCCCGCCTCGTTAGCCCACTTGCACCCCACCCTGTTACGCGCCCGTTCAGTCAGTCCAGGTACCGCCTGCGGGAAGTTGC
TGTCTCTGATAAGAGCCGATCTCAATGCATTGGGTGATTTACCCGTGGCGCAAGGTATCGAGAGGGAGCAGCAGATGCTT
GCCGATGGGGTGGCGCAGCTAGGTAAAGCGTGGGAGAGCTTATTAGTCGCCAATAGCAGTACGACAGAAAATAACAGTAC
GACAGAAAATAACAGCACGACGCGAGCGATACGGGAAGTACACCGCTCGCTGTTGCGTGATGGCACATTTCGTCAACGGC
TCTTGAGTCACATCGTAGCAGGTGAAAGCTGTGCGACAGCCATCGTCGCTACTGCGGCCTATTTCAGCCAGCAGTTGGCG
CTTGCTGCTAACACTTATCTACGTGAACGTGAGTTGGATATTCGAGACGTCAGTTTTCAATTGTTACAGCAGATTTATGG
TGAGCAACGTTTTCCCTCCCAGCAAGCATTGAGCGAAGACAGTCTCTGCATTGCCGATGAGTTAACCCCGAGCCAATTCC
TGGCGCTGGATAAACGTTATCTAAAAGGGTTGCTATTGGGCCGTGGTGGCAGTACTTCACATACGGTGATTCTGGCGCGT
TCATTCAATATTCCGACGCTCGTGGGTGTCGATGCTACGGCACTCCAGCCGTATCTCAATCAATCGCTGCAGATTGATGG
TGAGCTTGGGCTGGTGGTCTGTCTTTTGGATGAGCCGGTGCGCCGTTACTATCGGCAGGAGCAGTGGCTACACGACCAAC
TGCGTGAGCAGCAGAGCAGATACCAGAATATGCCTGGGCGTACTCTTGATGGTGTCAGAATGGTGGTGGCGGCTAACATC
ACCCATGCGGTAGAGGTGGAGGGTGCTTTCAATCAAGGTGCCGAATCCATCGGCTTGTTTCGTACTGAAATGTTGTATAT
GGATCGCGCAGCCGCGCCTTCAGAAGAGGAGCTTTATACCCTTTACGCTCAGGCCCTTGGCGCTGCCAAGGGCAAACCGA
TGATTATTCGCACCATCGATATTGGCGGTGATAAACCGGTGAGTTATCTGAATATTCCTGCGGAGAGCAACCCGTTTCTG
GGCTACCGCGCGGTACGTATTTATCACGAGTTTCTGTCGCTATTTCATACTCAATTGCGGGCGATCCTGCGGGCGTCAAT
GCATGGCCCGCTCAAAATCATGATCCCGATGATCTCCTCAATGGAGGAGATCCTGTGGGTGAAAGATCAACTGGCAGAGG
TGAAACAATCACTGCGTATCAATCATCTTCAGTTCGACGAAACTGTCCCCTTGGGTATGATGCTGGAAGTGCCTTCAGTG
ATGTTCATCATCGATCAATGCTGTGAGGAAATGGATTTTTTGAGTATTGGCAGTAACGACCTAACACAGTACCTGCTGGC
TGTTGATCGCGATAATGCCAAGGTGAGTGAGCACTATCACTGCTTGTCCCCGGCACTCCTACGCGCTCTCGATTACGCAG
TGTGCGAAGTACATCGCCACGGTAAGTGGATTGGCTTATGTGGTGAACTGGCGGCAAAAGACTCGGTATTACCTCTGTTG
GTAGCGATGGGGCTAGATGAGATCAGCATGAGTGCCTCTTTTATTGGCGCGACTAAAGCTCGCCTTGCCAAACTTGATCG
CGGCGAGTGCCGCCTGCTGCTCAACCGGGCGATGGCTTGCCGCACATCACGGGAAGTAGAACATCTGCTGGTGCAGTACG
ACGGGGAACAAGGTGATGAACCACTGATTATTCCTGAATGTATTACTCTCGGTGTGGATTGGCGTAGCAAAGAAGAGGTG
ATCAAAGGGATGGTGGATAACCTGCTGCTGGCTGGACGTTGCCGGTATCCGCGCAAACTGGCTGCGGATGTGTGGGCGCG
AGAAGCCCTTTTTTCCACCGGATTGGGCTTTGGGTTTGCCATTCCGCATGCTCAATCTGAGCATATTGAGCAGTCGACGA
TTAGCGTGGCCAAGTTGGCACAACCGGTGAATTGGGGAGAGGAAGACGCACTGTTTGTGATGATGTTAACCCTGAAAAAA
CAGGCTTCGGGTGACCAGCATATGCGTATTTTTTCCAAACTGGCGCGTCGGATCATGCACGAGGGTTTCCGCGAGGCGCT
GGTTAGCGCTGGGACGGCCCAGCAAATAGAAACGCTGCTGAAACACGAGCTCGAACTGTGA

Upstream 100 bases:

>100_bases
TGTGATTACATTTGTCCAGTATTTGACATTTTTATCTCATAGCCGCGAGGGGATAGCCGCTTTTATGCTGGTTACCAGCC
AGCGAGGGGAGGAAAGCCCA

Downstream 100 bases:

>100_bases
TTCTTTCATTTCAACATGACGGGTGCCGCGTCTGAGGTGGCACCCAGTGACCCTTATCGGATTACTTCAATAAGAATGAC
TTTCCTTGCTTAAGCACTAA

Product: PTS family enzyme I phosphohistidine subunit

Products: NA

Alternate protein names: MTP 2; Phosphoenolpyruvate-protein phosphotransferase; Enzyme I-Ani; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-like phosphotransferase enzyme IIA component; PTS system fructose-like EIIA component [H]

Number of amino acids: Translated: 846; Mature: 845

Protein sequence:

>846_residues
MTKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILLGDECQVTIEGEDEQAAFERL
SIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQML
ADGVAQLGKAWESLLVANSSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIVAGESCATAIVATAAYFSQQLA
LAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRYLKGLLLGRGGSTSHTVILAR
SFNIPTLVGVDATALQPYLNQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRMVVAANI
THAVEVEGAFNQGAESIGLFRTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIGGDKPVSYLNIPAESNPFL
GYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRINHLQFDETVPLGMMLEVPSV
MFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLSPALLRALDYAVCEVHRHGKWIGLCGELAAKDSVLPLL
VAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPLIIPECITLGVDWRSKEEV
IKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAQPVNWGEEDALFVMMLTLKK
QASGDQHMRIFSKLARRIMHEGFREALVSAGTAQQIETLLKHELEL

Sequences:

>Translated_846_residues
MTKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILLGDECQVTIEGEDEQAAFERL
SIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQML
ADGVAQLGKAWESLLVANSSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIVAGESCATAIVATAAYFSQQLA
LAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRYLKGLLLGRGGSTSHTVILAR
SFNIPTLVGVDATALQPYLNQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRMVVAANI
THAVEVEGAFNQGAESIGLFRTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIGGDKPVSYLNIPAESNPFL
GYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRINHLQFDETVPLGMMLEVPSV
MFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLSPALLRALDYAVCEVHRHGKWIGLCGELAAKDSVLPLL
VAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPLIIPECITLGVDWRSKEEV
IKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAQPVNWGEEDALFVMMLTLKK
QASGDQHMRIFSKLARRIMHEGFREALVSAGTAQQIETLLKHELEL
>Mature_845_residues
TKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILLGDECQVTIEGEDEQAAFERLS
IFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQMLA
DGVAQLGKAWESLLVANSSTTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIVAGESCATAIVATAAYFSQQLAL
AANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRYLKGLLLGRGGSTSHTVILARS
FNIPTLVGVDATALQPYLNQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRMVVAANIT
HAVEVEGAFNQGAESIGLFRTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIGGDKPVSYLNIPAESNPFLG
YRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRINHLQFDETVPLGMMLEVPSVM
FIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLSPALLRALDYAVCEVHRHGKWIGLCGELAAKDSVLPLLV
AMGLDEISMSASFIGATKARLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPLIIPECITLGVDWRSKEEVI
KGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAQPVNWGEEDALFVMMLTLKKQ
ASGDQHMRIFSKLARRIMHEGFREALVSAGTAQQIETLLKHELEL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI48994992, Length=842, Percent_Identity=63.4204275534442, Blast_Score=1070, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=846, Percent_Identity=42.1985815602837, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=453, Percent_Identity=38.6313465783664, Blast_Score=325, Evalue=1e-89,
Organism=Escherichia coli, GI1789193, Length=519, Percent_Identity=32.5626204238921, Blast_Score=251, Evalue=2e-67,
Organism=Escherichia coli, GI1787994, Length=412, Percent_Identity=27.1844660194175, Blast_Score=97, Evalue=5e-21,
Organism=Escherichia coli, GI1786951, Length=139, Percent_Identity=26.6187050359712, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 94294; Mature: 94162

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00369 PTS_HPR_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILL
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEE
GDECQVTIEGEDEQAAFERLSIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLR
CCCEEEEECCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQMLADGVAQLGKAWESLLVANSS
HCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIVAGESCATAIVATAAYFSQQLA
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRY
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCCCHHHHHHHHHHH
LKGLLLGRGGSTSHTVILARSFNIPTLVGVDATALQPYLNQSLQIDGELGLVVCLLDEPV
HHHHHCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHCCCEEECCCCCEEEEECCHHH
RRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRMVVAANITHAVEVEGAFNQGAESIGLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEECCCEEEEECCCHHCCHHHHHHH
RTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIGGDKPVSYLNIPAESNPFL
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCC
GYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
NHLQFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYH
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
CLSPALLRALDYAVCEVHRHGKWIGLCGELAAKDSVLPLLVAMGLDEISMSASFIGATKA
HHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
RLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPLIIPECITLGVDWRSKEEV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEHHHHEECCCCCCHHHH
IKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHH
QPVNWGEEDALFVMMLTLKKQASGDQHMRIFSKLARRIMHEGFREALVSAGTAQQIETLL
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KHELEL
HHHCCC
>Mature Secondary Structure 
TKQVTFICGLPNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIATNILL
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEE
GDECQVTIEGEDEQAAFERLSIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLR
CCCEEEEECCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ARSVSPGTACGKLLSLIRADLNALGDLPVAQGIEREQQMLADGVAQLGKAWESLLVANSS
HCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
TTENNSTTENNSTTRAIREVHRSLLRDGTFRQRLLSHIVAGESCATAIVATAAYFSQQLA
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELTPSQFLALDKRY
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHCCCCCEEEECCCCHHHHHHHHHHH
LKGLLLGRGGSTSHTVILARSFNIPTLVGVDATALQPYLNQSLQIDGELGLVVCLLDEPV
HHHHHCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHCCCEEECCCCCEEEEECCHHH
RRYYRQEQWLHDQLREQQSRYQNMPGRTLDGVRMVVAANITHAVEVEGAFNQGAESIGLF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHEECCCEEEEECCCHHCCHHHHHHH
RTEMLYMDRAAAPSEEELYTLYAQALGAAKGKPMIIRTIDIGGDKPVSYLNIPAESNPFL
HHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEEEECCCCCCCCC
GYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKDQLAEVKQSLRI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCC
NHLQFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYH
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
CLSPALLRALDYAVCEVHRHGKWIGLCGELAAKDSVLPLLVAMGLDEISMSASFIGATKA
HHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
RLAKLDRGECRLLLNRAMACRTSREVEHLLVQYDGEQGDEPLIIPECITLGVDWRSKEEV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEHHHHEECCCCCCHHHH
IKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHIEQSTISVAKLA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHHHH
QPVNWGEEDALFVMMLTLKKQASGDQHMRIFSKLARRIMHEGFREALVSAGTAQQIETLL
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
KHELEL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]