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The map label for this gene is mdh

Identifier: 51594812

GI number: 51594812

Start: 546394

End: 547332

Strand: Direct

Name: mdh

Synonym: YPTB0460

Alternate gene names: 51594812

Gene position: 546394-547332 (Clockwise)

Preceding gene: 51594809

Following gene: 51594814

Centisome position: 11.52

GC content: 47.71

Gene sequence:

>939_bases
ATGAAAGTTGCAGTTCTCGGTGCCGCTGGTGGGATTGGCCAGGCCCTCGCTCTTCTACTCAAGACCCAGCTTCCTTCAGG
TTCAGACCTCTCTTTATATGATATCGCGCCAGTAACGCCTGGTGTTGCTGTTGATCTGAGCCATATCCCTACAGCCGTTA
ACATTAAAGGCTTCAGCGGCGAAGATGCTACACCAGCCCTTCAAGGGGCAGATATTGTGCTGATTTCTGCTGGTGTCGCA
CGTAAACCTGGTATGGATCGTTCGGATCTGTTCAACGTCAATGCGGGCATTGTTCGTAATCTGGTTGAGCAAATTGCACG
TACTTGCCCGAACGCATTGATTGGTATCATCACTAACCCAGTCAACACAACCGTCGCTATTGCGGCTGAAGTGCTAAAAA
AAGCAGGCGTTTATGACAAGAATAAGCTGTTTGGTATTACCACTCTGGATACCATTCGCTCCAATACCTTTGTTGCCGAA
TTAAAAGGTAAGCAACCCCAGGATATCGAAGTGCCGGTTATTGGTGGCCACTCTGGTGTGACTATCTTGCCATTGCTATC
ACAGATCCCTGGCGTTAGCTTTACAGAACAAGAAGTTGCAGATTTGACCAAACGTATCCAGAACGCGGGTACCGAGGTTG
TAGAAGCTAAAGCCGGTGGCGGGTCCGCGACGCTGTCTATGGGGCAAGCTGCGGCACGTTTTGGTCTTTCTTTGGTACGT
GCCCTGCAAGGTGAAAGCAATGTTGTTGAATGCTCTTATGTTGAAGGCGATGGCAAATATGCCCGCTTCTTTGCTCAGCC
TATTCTACTGGGTAAAAACGGTGTCGCTGAACGTAAAGATATCGGTAAACTGAGTGCTTTTGAGCAACAAGCGCTGGAAA
ATATGCTTGATGTTTTACACAAAGATATCGAGTTAGGTGAGAAGTTCGTTAATCAATAA

Upstream 100 bases:

>100_bases
TCTAGCTGTTAATTTATTGTGTTTAAAATCACAATAAATTAAGGTGCATAACTAGATGAATTCACGGCAAATTTAAATTA
AACATAATAAGGAGTATAGG

Downstream 100 bases:

>100_bases
TATTCCCTTCGGCCTTGACGTTGCAGGGGGTTAGCTGCAAGCGTTACTCGGCCCATCCATGGGCCTCGCTTCTGCGAGGC
CGCTGCAAGCCGCGTTCAAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKVAVLGAAGGIGQALALLLKTQLPSGSDLSLYDIAPVTPGVAVDLSHIPTAVNIKGFSGEDATPALQGADIVLISAGVA
RKPGMDRSDLFNVNAGIVRNLVEQIARTCPNALIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGITTLDTIRSNTFVAE
LKGKQPQDIEVPVIGGHSGVTILPLLSQIPGVSFTEQEVADLTKRIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVR
ALQGESNVVECSYVEGDGKYARFFAQPILLGKNGVAERKDIGKLSAFEQQALENMLDVLHKDIELGEKFVNQ

Sequences:

>Translated_312_residues
MKVAVLGAAGGIGQALALLLKTQLPSGSDLSLYDIAPVTPGVAVDLSHIPTAVNIKGFSGEDATPALQGADIVLISAGVA
RKPGMDRSDLFNVNAGIVRNLVEQIARTCPNALIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGITTLDTIRSNTFVAE
LKGKQPQDIEVPVIGGHSGVTILPLLSQIPGVSFTEQEVADLTKRIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVR
ALQGESNVVECSYVEGDGKYARFFAQPILLGKNGVAERKDIGKLSAFEQQALENMLDVLHKDIELGEKFVNQ
>Mature_312_residues
MKVAVLGAAGGIGQALALLLKTQLPSGSDLSLYDIAPVTPGVAVDLSHIPTAVNIKGFSGEDATPALQGADIVLISAGVA
RKPGMDRSDLFNVNAGIVRNLVEQIARTCPNALIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGITTLDTIRSNTFVAE
LKGKQPQDIEVPVIGGHSGVTILPLLSQIPGVSFTEQEVADLTKRIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVR
ALQGESNVVECSYVEGDGKYARFFAQPILLGKNGVAERKDIGKLSAFEQQALENMLDVLHKDIELGEKFVNQ

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 1 family [H]

Homologues:

Organism=Homo sapiens, GI21735621, Length=311, Percent_Identity=56.2700964630225, Blast_Score=334, Evalue=6e-92,
Organism=Homo sapiens, GI47059044, Length=317, Percent_Identity=23.9747634069401, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136809, Length=317, Percent_Identity=23.9747634069401, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI5031857, Length=319, Percent_Identity=24.4514106583072, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI260099723, Length=319, Percent_Identity=24.4514106583072, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789632, Length=312, Percent_Identity=89.7435897435898, Blast_Score=568, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI17554310, Length=312, Percent_Identity=55.7692307692308, Blast_Score=325, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6322765, Length=315, Percent_Identity=48.5714285714286, Blast_Score=263, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6320125, Length=339, Percent_Identity=40.117994100295, Blast_Score=196, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6324446, Length=361, Percent_Identity=36.5650969529086, Blast_Score=179, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24647881, Length=312, Percent_Identity=56.7307692307692, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI24663599, Length=318, Percent_Identity=49.685534591195, Blast_Score=280, Evalue=6e-76,
Organism=Drosophila melanogaster, GI24663595, Length=298, Percent_Identity=43.9597315436242, Blast_Score=244, Evalue=6e-65,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010097
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 32625; Mature: 32625

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00068 MDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPSGSDLSLYDIAPVTPGVAVDLSHIPTAVNIKGFSG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEHHHCCCEEEEECCCC
EDATPALQGADIVLISAGVARKPGMDRSDLFNVNAGIVRNLVEQIARTCPNALIGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHCCHHEEEEECCC
VNTTVAIAAEVLKKAGVYDKNKLFGITTLDTIRSNTFVAELKGKQPQDIEVPVIGGHSGV
CHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCCCEEEEEECCCCCCCCEEEEECCCCCC
TILPLLSQIPGVSFTEQEVADLTKRIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
ALQGESNVVECSYVEGDGKYARFFAQPILLGKNGVAERKDIGKLSAFEQQALENMLDVLH
HHCCCCCEEEEEEEECCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KDIELGEKFVNQ
HHHHHHHHHHCC
>Mature Secondary Structure
MKVAVLGAAGGIGQALALLLKTQLPSGSDLSLYDIAPVTPGVAVDLSHIPTAVNIKGFSG
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEHHHCCCEEEEECCCC
EDATPALQGADIVLISAGVARKPGMDRSDLFNVNAGIVRNLVEQIARTCPNALIGIITNP
CCCCCCCCCCCEEEEECCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHCCHHEEEEECCC
VNTTVAIAAEVLKKAGVYDKNKLFGITTLDTIRSNTFVAELKGKQPQDIEVPVIGGHSGV
CHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHCCCEEEEEECCCCCCCCEEEEECCCCCC
TILPLLSQIPGVSFTEQEVADLTKRIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
ALQGESNVVECSYVEGDGKYARFFAQPILLGKNGVAERKDIGKLSAFEQQALENMLDVLH
HHCCCCCEEEEEEEECCCHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KDIELGEKFVNQ
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA