Click here to switch to the map view.

The map label for this gene is purA

Identifier: 51594782

GI number: 51594782

Start: 513918

End: 515216

Strand: Direct

Name: purA

Synonym: YPTB0430

Alternate gene names: 51594782

Gene position: 513918-515216 (Clockwise)

Preceding gene: 51594781

Following gene: 51594783

Centisome position: 10.83

GC content: 50.96

Gene sequence:

>1299_bases
ATGGGTAAGAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAAGGTAAGGGCAAGGTCGTTGACCTGCTGACTGAACG
GGCTAAATATGTTGTGCGCTATCAAGGTGGCCATAACGCGGGCCATACTTTGGTTATCAACGGTGAGAAAACCGTTCTTC
ATTTAATTCCCTCAGGTATTCTACGTGAAAACGTAATCAGCATCATCGGCAACGGTGTTGTACTGGCTCCTGACGCGTTG
ATGAAAGAGATGACAGAGCTTGAAGCGCGCGGTGTGCCTGTTCGTGAGCGACTGTTACTCTCCGAAGCATGCCCATTGAT
CCTGCCTTATCATGTGGCTCTGGATAATGCGCGTGAAAAAGCACGTGGTGCAAAAGCTATCGGTACTACCGGTCGTGGTA
TTGGCCCTGCCTATGAAGATAAAGTTGCTCGCCGCGGCCTACGCGTCAGTGACTTGTTCAATAAAGAAACCTTCGCTATC
AAGCTGAAAGAAATTGTGGAATACCACAACTTCCAGTTGGTGCATTACTACAAAGAAGCGGCGGTTGACTACCAAAAAGT
GCTGGATGATGTATTGGCTATCGCCGATATTCTGACGGCCATGGTAGTTGATGTTTCTGAATTGTTGGACAATGCCCGTA
AGCAAGGCGAGTTGATCATGTTCGAAGGCGCACAAGGCACCTTGCTGGACATCGACCACGGTACTTATCCGTATGTCACC
TCATCTAACACCACCGCGGGTGGCGTAGCGACAGGCTCTGGCCTGGGTCCACGTTATGTTGATTACGTGCTGGGTATCGT
AAAAGCCTATTCTACCCGTGTAGGTGCAGGTCCATTCCCTACCGAACTGAACGATGAAACTGGTGAGTTCCTGCGCAAGC
AAGGTAACGAATATGGCGCGACTACCGGCCGTAGCCGTCGTACTGGCTGGTTGGATATCGTAGCCGTTCGCCGTGCAGTG
CAGATCAACTCCCTGTCTGGTTTCTGCATGACCAAACTGGATGTGCTGGATGGTCTGAAAGAAGTGAAGCTCTGTGTTGG
CTACCGTATGCCTGATGGCCGCGAAGTTGATACAACGCCACTGGCTGCAGAAGGCTGGGAAGGCATCGAGCCAATCTACG
AAACCATGCCAGGTTGGTCAGAAACCACCTTTGGTGTGAAGGAACACAGTAAGTTGCCACAGGCAGCCCTGAACTACATT
CAACGTGTAGAAGAGCTGACAGGTGTGCCGATTGATATCATCTCTACCGGCCCTGATCGTGACGAAACCATGATCCTGCG
TGATCCATTTGATGCATAA

Upstream 100 bases:

>100_bases
AAGGTAAGCGCAAAAATTGTTCAGTTATGCGTTAAAAAGTACTGAAAGCCGCCGTATGAGATGTTAGAATCCTTTTTTAA
GCAACCTGGTGATTTTTGAG

Downstream 100 bases:

>100_bases
TTAACTGGCTTACGGCCAAGTTATCATCATTAATGAGATATCAGCATTAAAATGTACGGGGTTAACGCGTATATTTGTGT
TCGAAACAATAAACCGGGCT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MGKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGILRENVISIIGNGVVLAPDAL
MKEMTELEARGVPVRERLLLSEACPLILPYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVSDLFNKETFAI
KLKEIVEYHNFQLVHYYKEAAVDYQKVLDDVLAIADILTAMVVDVSELLDNARKQGELIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVATGSGLGPRYVDYVLGIVKAYSTRVGAGPFPTELNDETGEFLRKQGNEYGATTGRSRRTGWLDIVAVRRAV
QINSLSGFCMTKLDVLDGLKEVKLCVGYRMPDGREVDTTPLAAEGWEGIEPIYETMPGWSETTFGVKEHSKLPQAALNYI
QRVEELTGVPIDIISTGPDRDETMILRDPFDA

Sequences:

>Translated_432_residues
MGKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGILRENVISIIGNGVVLAPDAL
MKEMTELEARGVPVRERLLLSEACPLILPYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVSDLFNKETFAI
KLKEIVEYHNFQLVHYYKEAAVDYQKVLDDVLAIADILTAMVVDVSELLDNARKQGELIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVATGSGLGPRYVDYVLGIVKAYSTRVGAGPFPTELNDETGEFLRKQGNEYGATTGRSRRTGWLDIVAVRRAV
QINSLSGFCMTKLDVLDGLKEVKLCVGYRMPDGREVDTTPLAAEGWEGIEPIYETMPGWSETTFGVKEHSKLPQAALNYI
QRVEELTGVPIDIISTGPDRDETMILRDPFDA
>Mature_431_residues
GKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGILRENVISIIGNGVVLAPDALM
KEMTELEARGVPVRERLLLSEACPLILPYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVSDLFNKETFAIK
LKEIVEYHNFQLVHYYKEAAVDYQKVLDDVLAIADILTAMVVDVSELLDNARKQGELIMFEGAQGTLLDIDHGTYPYVTS
SNTTAGGVATGSGLGPRYVDYVLGIVKAYSTRVGAGPFPTELNDETGEFLRKQGNEYGATTGRSRRTGWLDIVAVRRAVQ
INSLSGFCMTKLDVLDGLKEVKLCVGYRMPDGREVDTTPLAAEGWEGIEPIYETMPGWSETTFGVKEHSKLPQAALNYIQ
RVEELTGVPIDIISTGPDRDETMILRDPFDA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=43.4272300469484, Blast_Score=328, Evalue=5e-90,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=41.8224299065421, Blast_Score=315, Evalue=4e-86,
Organism=Homo sapiens, GI40316944, Length=400, Percent_Identity=41.5, Blast_Score=288, Evalue=7e-78,
Organism=Escherichia coli, GI1790620, Length=432, Percent_Identity=88.6574074074074, Blast_Score=793, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=42.8904428904429, Blast_Score=335, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=42.8904428904429, Blast_Score=334, Evalue=4e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=434, Percent_Identity=41.705069124424, Blast_Score=337, Evalue=2e-93,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=42.7553444180523, Blast_Score=341, Evalue=6e-94,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47279; Mature: 47147

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGI
CCCCEEEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRENVISIIGNGVVLAPDALMKEMTELEARGVPVRERLLLSEACPLILPYHVALDNAREK
HHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHH
ARGAKAIGTTGRGIGPAYEDKVARRGLRVSDLFNKETFAIKLKEIVEYHNFQLVHYYKEA
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCHHEEEHHHHHHHHCEEEEEHHHHH
AVDYQKVLDDVLAIADILTAMVVDVSELLDNARKQGELIMFEGAQGTLLDIDHGTYPYVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGLGPRYVDYVLGIVKAYSTRVGAGPFPTELNDETGEFLRKQGNEYGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCC
TTGRSRRTGWLDIVAVRRAVQINSLSGFCMTKLDVLDGLKEVKLCVGYRMPDGREVDTTP
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LAAEGWEGIEPIYETMPGWSETTFGVKEHSKLPQAALNYIQRVEELTGVPIDIISTGPDR
CCCCCCCCCCHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
DETMILRDPFDA
CCEEEECCCCCC
>Mature Secondary Structure 
GKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGI
CCCEEEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRENVISIIGNGVVLAPDALMKEMTELEARGVPVRERLLLSEACPLILPYHVALDNAREK
HHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHH
ARGAKAIGTTGRGIGPAYEDKVARRGLRVSDLFNKETFAIKLKEIVEYHNFQLVHYYKEA
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCHHEEEHHHHHHHHCEEEEEHHHHH
AVDYQKVLDDVLAIADILTAMVVDVSELLDNARKQGELIMFEGAQGTLLDIDHGTYPYVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGLGPRYVDYVLGIVKAYSTRVGAGPFPTELNDETGEFLRKQGNEYGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCC
TTGRSRRTGWLDIVAVRRAVQINSLSGFCMTKLDVLDGLKEVKLCVGYRMPDGREVDTTP
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LAAEGWEGIEPIYETMPGWSETTFGVKEHSKLPQAALNYIQRVEELTGVPIDIISTGPDR
CCCCCCCCCCHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
DETMILRDPFDA
CCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA