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The map label for this gene is narH [H]

Identifier: 50841993

GI number: 50841993

Start: 557396

End: 558919

Strand: Reverse

Name: narH [H]

Synonym: PPA0509

Alternate gene names: 50841993

Gene position: 558919-557396 (Counterclockwise)

Preceding gene: 50841994

Following gene: 50841992

Centisome position: 21.83

GC content: 58.99

Gene sequence:

>1524_bases
ATGAAAGTCATGGCACAGATCGCGATGGTGATGAACCTTGACAAGTGCATCGGCTGCCACACCTGCTCAGTGACATGCAA
ACAGGCCTGGACAAACCGTGAGGGCACGGAATACATGTGGTTTAACAACGTCGAGACTCGGCCCGGCGTTGGCTACCCGC
GCGGCTGGGAAGATCAGGAACGCTGGAGGGGAGGCTGGAAGCGCACCCGTTCCGGGAAACTGGTCCCCTATCAGGGAGGG
CGTTTGGCCACCTTAGGCAAGATTTTCGCCAATCCGAACCTGCCCGCCAAGAAGGACTACTACGAGCCGTGGACTTACGA
ATACGACCAACTACTGGCGTCCCCATCGACTCAGCACATTCCCACTGCCCGAGCAAAATCAGCCCTCACGGGTGAGCACA
AGGACACCATCGACTGGGGCCCCAACTGGGATGACGATCTTGGTGGCTCCATGGAAACCCTCGATCAGGACCCAGTCCTA
CACCAAATGAACCTCACGGTACGCAAGTCCATTGAGGATTCATTCATGTTCTATCTGCCGCGCATCTGCGAACACTGCCT
CAACCCGACATGTGTTTCTGCCTGCCCATCTGGGGCGATCTACAAGCGTGCCGAGGATGGAATCGTCCTTGTCGACCAGG
ACCATTGTCGCGGCTGGCGCATGTGTGTGTCCGGCTGCCCGTATAAGAAGGTGTTCTTCAACCATCACAGCGGCAAAGCC
GAGAAATGCACACTGTGCTATCCACGGCTGGAAGTCGGACAGCCAACAGTGTGCTCGGAAACTTGCGTTGGACGACTGCG
CTACCTGGGGGTGCTCCTCTACGACGCCGACCACGTCACCTGGGCCGCATCTCAGCCCGACCCACGTACCCTGTACGCCG
CCCAGAGAGACATCCTCCTCAACCCCAACGACCCCGAAATCATCACACAAGCCAAGGCCAACGGCGTCCCACACTCTTGG
ATCAAAGCAGCCCAGGCGTCACCGGTATGGCAGCTCATCAGCCGCTATCAGATTGCCCTACCCCTGCACCCTGAGTACCG
AACACTGCCGATGGTCTGGTACGTACCACCTCTGTCACCAGTCGTCGACGCTGTCAGCGCTTCGGGCAACGACGGGGAAG
ATCACAGAGTGCTGTTGGCCGCGATTTCCCAGATGCGCATTCCGCTTGAGTATCTCGCCGGCCTATTCACTGCTGGAGCC
ACGGCTCCAGTTGAGCTCTCACTTCGTCGTCTGGCTGCAATGCGCTCCTACATGCGAGACATCTTTTTGGGGAATGAAGC
AGACGAAACGATCCCCGAGGCAGTGGACATGACCGGCGAGGACATACAGAGTATGTACCGACTGCTGTCCATCGCCAAGT
ACGACGACCGCTACGTCATTCCCACCACCTGCAGCGGCCTACCCGACGGAATCGCCGACCTGGGTCGCTGCCCCGTGAGT
GGAAGCCTCGAAGCCTTCCACAGCATCTCCTCCCCGGTCAGCGCCCCAGAGGGCACACCCACCTTGCCGCTGGAGGTGAT
CTGA

Upstream 100 bases:

>100_bases
ACGCCCAGCTCAGTTACGCCTTCAACTACTACGGCCCCACCGGCAACCAACGTGACGAAGTGACGCTCATTAGGCGTCGC
GGCCAGGAGGTGACGTTCTG

Downstream 100 bases:

>100_bases
GGTGGGTGTCTTCTTAGGCGCACCACGCATACCAGTGTCGACCCACGTGGATGCCTCTCTTGCCCAGCGCCGCGTGGTTC
ACATGGCGACGAGTCTGCTA

Product: nitrate reductase beta chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKVMAQIAMVMNLDKCIGCHTCSVTCKQAWTNREGTEYMWFNNVETRPGVGYPRGWEDQERWRGGWKRTRSGKLVPYQGG
RLATLGKIFANPNLPAKKDYYEPWTYEYDQLLASPSTQHIPTARAKSALTGEHKDTIDWGPNWDDDLGGSMETLDQDPVL
HQMNLTVRKSIEDSFMFYLPRICEHCLNPTCVSACPSGAIYKRAEDGIVLVDQDHCRGWRMCVSGCPYKKVFFNHHSGKA
EKCTLCYPRLEVGQPTVCSETCVGRLRYLGVLLYDADHVTWAASQPDPRTLYAAQRDILLNPNDPEIITQAKANGVPHSW
IKAAQASPVWQLISRYQIALPLHPEYRTLPMVWYVPPLSPVVDAVSASGNDGEDHRVLLAAISQMRIPLEYLAGLFTAGA
TAPVELSLRRLAAMRSYMRDIFLGNEADETIPEAVDMTGEDIQSMYRLLSIAKYDDRYVIPTTCSGLPDGIADLGRCPVS
GSLEAFHSISSPVSAPEGTPTLPLEVI

Sequences:

>Translated_507_residues
MKVMAQIAMVMNLDKCIGCHTCSVTCKQAWTNREGTEYMWFNNVETRPGVGYPRGWEDQERWRGGWKRTRSGKLVPYQGG
RLATLGKIFANPNLPAKKDYYEPWTYEYDQLLASPSTQHIPTARAKSALTGEHKDTIDWGPNWDDDLGGSMETLDQDPVL
HQMNLTVRKSIEDSFMFYLPRICEHCLNPTCVSACPSGAIYKRAEDGIVLVDQDHCRGWRMCVSGCPYKKVFFNHHSGKA
EKCTLCYPRLEVGQPTVCSETCVGRLRYLGVLLYDADHVTWAASQPDPRTLYAAQRDILLNPNDPEIITQAKANGVPHSW
IKAAQASPVWQLISRYQIALPLHPEYRTLPMVWYVPPLSPVVDAVSASGNDGEDHRVLLAAISQMRIPLEYLAGLFTAGA
TAPVELSLRRLAAMRSYMRDIFLGNEADETIPEAVDMTGEDIQSMYRLLSIAKYDDRYVIPTTCSGLPDGIADLGRCPVS
GSLEAFHSISSPVSAPEGTPTLPLEVI
>Mature_507_residues
MKVMAQIAMVMNLDKCIGCHTCSVTCKQAWTNREGTEYMWFNNVETRPGVGYPRGWEDQERWRGGWKRTRSGKLVPYQGG
RLATLGKIFANPNLPAKKDYYEPWTYEYDQLLASPSTQHIPTARAKSALTGEHKDTIDWGPNWDDDLGGSMETLDQDPVL
HQMNLTVRKSIEDSFMFYLPRICEHCLNPTCVSACPSGAIYKRAEDGIVLVDQDHCRGWRMCVSGCPYKKVFFNHHSGKA
EKCTLCYPRLEVGQPTVCSETCVGRLRYLGVLLYDADHVTWAASQPDPRTLYAAQRDILLNPNDPEIITQAKANGVPHSW
IKAAQASPVWQLISRYQIALPLHPEYRTLPMVWYVPPLSPVVDAVSASGNDGEDHRVLLAAISQMRIPLEYLAGLFTAGA
TAPVELSLRRLAAMRSYMRDIFLGNEADETIPEAVDMTGEDIQSMYRLLSIAKYDDRYVIPTTCSGLPDGIADLGRCPVS
GSLEAFHSISSPVSAPEGTPTLPLEVI

Specific function: The Nitrate Reductase Enzyme Complex Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In The Formation Of Iron-Sulfur Centers. Electrons A

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=466, Percent_Identity=56.2231759656652, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI1787740, Length=478, Percent_Identity=54.18410041841, Blast_Score=569, Evalue=1e-163,
Organism=Escherichia coli, GI1787872, Length=133, Percent_Identity=39.0977443609023, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787122, Length=133, Percent_Identity=38.3458646616541, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789370, Length=102, Percent_Identity=39.2156862745098, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1787749, Length=80, Percent_Identity=38.75, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI2367345, Length=96, Percent_Identity=34.375, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790326, Length=81, Percent_Identity=41.9753086419753, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=36.046511627907, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 56565; Mature: 56565

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMAQIAMVMNLDKCIGCHTCSVTCKQAWTNREGTEYMWFNNVETRPGVGYPRGWEDQE
CHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHH
RWRGGWKRTRSGKLVPYQGGRLATLGKIFANPNLPAKKDYYEPWTYEYDQLLASPSTQHI
HHCCCHHCCCCCCEEECCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCC
PTARAKSALTGEHKDTIDWGPNWDDDLGGSMETLDQDPVLHQMNLTVRKSIEDSFMFYLP
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHCHHHHHHHHHHHHHHHH
RICEHCLNPTCVSACPSGAIYKRAEDGIVLVDQDHCRGWRMCVSGCPYKKVFFNHHSGKA
HHHHHHCCCHHHHHCCCCCEEEECCCCEEEEECHHCCHHHHHHCCCCCHHHEEECCCCCC
EKCTLCYPRLEVGQPTVCSETCVGRLRYLGVLLYDADHVTWAASQPDPRTLYAAQRDILL
CEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCEEEECCCCCCCEEEECCCCEEE
NPNDPEIITQAKANGVPHSWIKAAQASPVWQLISRYQIALPLHPEYRTLPMVWYVPPLSP
CCCCCCEEEEHHCCCCCHHHHHHHCCCHHHHHHHCCEEEEECCCCCCCCCEEEECCCCHH
VVDAVSASGNDGEDHRVLLAAISQMRIPLEYLAGLFTAGATAPVELSLRRLAAMRSYMRD
HHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IFLGNEADETIPEAVDMTGEDIQSMYRLLSIAKYDDRYVIPTTCSGLPDGIADLGRCPVS
HHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHCCCCCC
GSLEAFHSISSPVSAPEGTPTLPLEVI
CCHHHHHHHCCCCCCCCCCCCCCCEEC
>Mature Secondary Structure
MKVMAQIAMVMNLDKCIGCHTCSVTCKQAWTNREGTEYMWFNNVETRPGVGYPRGWEDQE
CHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCHHH
RWRGGWKRTRSGKLVPYQGGRLATLGKIFANPNLPAKKDYYEPWTYEYDQLLASPSTQHI
HHCCCHHCCCCCCEEECCCCCEEHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCC
PTARAKSALTGEHKDTIDWGPNWDDDLGGSMETLDQDPVLHQMNLTVRKSIEDSFMFYLP
CCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHHHCHHHHHHHHHHHHHHHH
RICEHCLNPTCVSACPSGAIYKRAEDGIVLVDQDHCRGWRMCVSGCPYKKVFFNHHSGKA
HHHHHHCCCHHHHHCCCCCEEEECCCCEEEEECHHCCHHHHHHCCCCCHHHEEECCCCCC
EKCTLCYPRLEVGQPTVCSETCVGRLRYLGVLLYDADHVTWAASQPDPRTLYAAQRDILL
CEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCEEEECCCCCCCEEEECCCCEEE
NPNDPEIITQAKANGVPHSWIKAAQASPVWQLISRYQIALPLHPEYRTLPMVWYVPPLSP
CCCCCCEEEEHHCCCCCHHHHHHHCCCHHHHHHHCCEEEEECCCCCCCCCEEEECCCCHH
VVDAVSASGNDGEDHRVLLAAISQMRIPLEYLAGLFTAGATAPVELSLRRLAAMRSYMRD
HHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IFLGNEADETIPEAVDMTGEDIQSMYRLLSIAKYDDRYVIPTTCSGLPDGIADLGRCPVS
HHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHCCCCCC
GSLEAFHSISSPVSAPEGTPTLPLEVI
CCHHHHHHHCCCCCCCCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]